Research and Practical Medicine Journal最新文献

{"title":"Correction of thrombohemorrhagic syndrome in patients with widespread soft tissue phlegmon and sepsis","authors":"E. A. Tseimakh, V. Zinchenko, O. Lavrinenko, V. Chernenko, A. Kalashnikov, E. Shalabod, M. E. M. Tseimakh","doi":"10.17709/2410-1893-2022-9-1-7","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-7","url":null,"abstract":"Purpose of the study. Analysis of the effectiveness of cryoprecipitate – antienzymatic with common soft tissue phlegmon and sepsis.Patients and methods. To achieve the intended goal, a study of the results of treatment of 92 patients with a diagnosis of advanced soft tissue phlegmon and sepsis was performed. All patients underwent a comprehensive diagnostic study with an in-depth study of the indicators of the hemostasis system. For a comparative assessment, 2 groups of patients were identified. Group 1 (46 (50 %) patients – the main one) – received cryoprecipitate-antienzymatic therapy as part of complex treatment, group 2 (46 (50 %) patients – comparisons) – received only the generally accepted basic treatment.Results. An in-depth study of the hemostasis system in patients with widespread soft tissue phlegmon and sepsis indicated the development of thrombohemorrhagic syndrome, which was manifested by depletion of both the coagulation and anticoagulation systems of the blood, a drop in AT III, an increase in thrombinemia and fibrinolysis depression. In order to correct thrombohemorrhagic syndrome, a cryoprecipitate-antienzymatic complex was included in the complex treatment of patients with widespread soft tissue phlegmons and sepsis, aimed at unblocking microcirculation in inflammatory foci and parenchymal organs, to improve the penetration of antibacterial drugs and other drugs into these foci, thereby stopping inflammation and preventing the increase of multiple organ failure. The effectiveness of the cryoprecipitate-antienzymatic complex was evaluated based on clinical data of the course of the purulent – inflammatory process and the results of indicators of the hemocoagulation and fibrinolysis system on 8–10 days from the start of therapy.Conclusion. The use of cryoprecipitate-antienzymatic complex, which includes freshly frozen plasma (FFP), heparin, proteinase inhibitors, in patients with widespread soft tissue phlegmon and sepsis effectively eliminates thrombohemorrhagic syndrome, which improves microcirculation in purulent – inflammatory foci and affected parenchymal organs, improving the flow of antibacterial drugs into these foci. At the same time, there is an improvement in the course of the wound process, so the wounds are cleaned faster from necrotic masses and filled with healthy granulation tissue, which makes it possible to perform plastic closure of the wound defect 1.6 times faster. Mortality is reduced by 2.2 times and the number of thrombotic and thromboembolic complications in the structure of deaths is reduced.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122783074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of diabetes mellitus on levels of sex hormones and their receptors in tumor tissues in female rats with Guerin’s carcinoma","authors":"E. Frantsiyants, V. Bandovkina, I. Kaplieva, E. Surikova, Y. Pogorelova, N. Cheryarina, I. Kotieva, M. Morozova, A. Shikhlyarova","doi":"10.17709/2410-1893-2022-9-1-2","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-2","url":null,"abstract":"Purpose of the study. Analysis of the effect of DM on levels of sex hormones and their receptors in tumor and perifocal tissues in outbred white female rats.Materials and methods. Outbred white female rats were divided into 2 groups, 18 rats each: control group – with transplantable Guerin’s carcinoma, main group – with transplantable Guerin’s carcinoma growing in presence of DM. DM was reproduces in animals by the single intraperitoneal alloxan injection (150 mg/kg body weight). Animals were killed after 10 days of the tumor growth. Levels of hormones (estradiol, testosterone, progesterone and prolactin) and their receptors (RЕα, RЕβ, RA and RP4) were measured by ELISA in tumor and perifocal tissues in animals of the control and main groups.Results. Female rats with Guerin’s carcinoma growing in presence of DM showed an increase in glucose in tumor and perifocal tissues by 1.8 times (р < 0.05) and 7.6 times, respectively, compared to the levels in the control group. Levels of E2 were increased, respectively, by 2.4 and 9.5 times; P4 – by 2.1 and 3.0 times; PRL – by 2.7 and 4.4 times. T was increased in perifocal tissues by 2.2 times and was unchanged in tumor tissues. RЕα and RP4 were elevated by 3.9 and 3.0 times, respectively, only in tumor tissues, and RA by 3.9 times only in perifocal tissues. The REα/RЕβ ratio was increased only in the tumor by 3.2 times. The Е2/RЕβ ratio was increased in tumor and perifocal tissues by 2.0 and 9.6 times, respectively. The T/RA ratio was decreased in the tumor and its perifocal area by 1.4 (р < 0.05) and 2.0 times, respectively. The opposite changes were observed in the Е2/RЕα and P4/RP4 ratios: a decrease in the tumor by 1.6 and 1.4 times, respectively (р < 0.05), and an increase in the perifocal area by 9.8 and 2.5 times, respectively.Conclusion. Female rats with Guerin’s carcinoma growing in presence of DM demonstrated local hyperglycemia, changes in the levels of sex steroids and a misbalance in their receptors in tumor and perifocal tissues causing active metastasis and reduced average survival of animals. ","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133670369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inflammatory myofibroblastic liver tumor","authors":"A. Kaprin, S. A. Ivanov, A. Nevolskikh, L. Petrov, A. Isaeva, R. F. Zibirov, A. Izmailov","doi":"10.17709/2410-1893-2022-9-1-11","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-11","url":null,"abstract":"Inflammatory myofibroblastic tumors (inflammatory myofibroblastic tumors) IMT in the clinical practice of an oncologist are very rare diagnostic findings. Currently, the bulk of scientific publications about IMT are devoted to reviews of clinical cases. If initially IMT were considered as pseudo-tumors, now they are classified as intermediate fibroblastic/myofibroblastic tumors according to the WHO histological classification. Management of patients with hepatic IMT are debatable today. Hepatic IMT are mostly benign lesions and characterized by spontaneous regression without any treatment. However, sometimes therapeutic and surgical treatment of these lesions is necessary. Many authors recommend surgical treatment, as with conservative treatment, some patients develop relapses. This rare observation demonstrates our experience of liver resection for inflammatory myofibroblastic liver tumor in 76 year-old patient. ","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125274191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological support in the perioperative period of implantation of a polypropylene mesh prosthesis (experimental study)","authors":"E. Lukoyanychev, S. G. Izmajlov, A. E. Leontev, A. A. Mironov, V. O. Nikolskij, D. A. Evsjukov, V. Emelyanov","doi":"10.17709/2410-1893-2022-9-1-8","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-8","url":null,"abstract":"Purpose of the study. was to improve the technology of prosthetic repair of the anterior abdominal wall patients with hernias by means of pharmacological regulation of the local and systemic inflammatory response and stimulation of reparative regeneration.Materials and methods. The study was carried out on 52 pure line rats in vivarium. All animals have on-lay implantation of a polypropylene mesh imlantant. Group I have no specific pharmacological treatment receive (0.9 % sodium chloride solution); group II – receive solution of xymedon, group III – potassium orotate, group IV – methyluracil.Results. Identified that all investigated drugs of the pyrimidine series (xymedon, potassium orotate, methyluracil) have a significant effect on the local and systemic inflammatory process. The growth of IL 10 and TNF-a are associated with an increase in the area of liquid inclusions. The use of potassium orotate and methyluracil in the postoperative period is inappropriate, as it is associated with an increased risk of hemorrhage and acute paraprosthetic fluid accumulations. Taking xymedon after surgery is associated with an accelerated growth of blood vessels and granulation tissue, a decrease in fluid inclusions, but is associated with an insignificant increase in the risk of tissue hemorrhage.Conclusion. Pharmacological regulation of inflammatory and regenerative processes with xymedon in the perioperative period of prosthetic repair could guide the development of surgical treatment of patients with abdominal hernias, which requires further clinical study.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121629576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of growth of colorectal cancer patient-derived subcutaneous xenografts using combined Wnt signaling pathway inhibitor and 5‑fluorouracil","authors":"A. Goncharova, A. V. Galina, D. Khodakova, G. Y. Egorov, A. Maksimov, E. Kolesnikov, E. F. Komarova, A. Kiblitskaya, E. Zaikina, L. Z. Kurbanova, M. V. Mindar","doi":"10.17709/2410-1893-2022-9-1-3","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-3","url":null,"abstract":"Purpose of the study. Was to analyze antitumor efficacy of the XAV 939 Wnt signaling pathway inhibitor and its combination with 5 fluorouracil in subcutaneous xenografts derived from patients with colorectal cancer.Materials and methods. Antitumor efficacy of the agents and their combination was studied in xenografts derived from patients with colorectal cancer and subcutaneously implanted in immunodeficient Balb/c Nude mice. All animals with tumors were divided into 4 groups (n = 5): group 1 received 5 fluorouracil 25 mg/kg, group 2 – XAV 939 25 mg/kg, group 3–5 fluorouracil and XAV 939 combination at the same dosages, group 4 was control. Criteria for the efficacy of the tested agents and their combination included tumor growth rate and tumor growth inhibition rate (TGI %).Results. The mean volumes of xenografts and tumor growth rate in the group receiving a combination of 5 fluorouracil and XAV 939 were 335.2 ± 40.7 mm3 , being lower than the averages of xenografts in controls – 609.3 ± 69.5 mm3 (p < 0.05). The mean volumes of xenografts in the group receiving 5 fluorouracil monotherapy were 601.9 ± 45.5 mm3 , in the group with the XAV 939 monotherapy – 527.9 ± 258.6 mm3 . The highest TGI (44.99 %) was registered in the group receiving a combination of 5 fluorouracil and XAV 939.Conclusion. The study revealed the ability of combined XAV 939 Wnt signaling pathway inhibitor and 5 fluorouracil to inhibit the growth of subcutaneous xenografts derived from patients with colorectal cancer.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131866634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mast cell hyperactivity as the factor in the pathogenesis of male infertility","authors":"N. Kulchenko","doi":"10.17709/2410-1893-2022-9-1-10","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-10","url":null,"abstract":"The proportion of men with impaired sperm fertility is increasing every year, which is one of the factors in the decline in fertility and is becoming both a medical and social problem. Modern diagnostic methods make it possible to recognize many factors of male infertility: genetic, endocrine, infectious, extra-genital, etc. However, despite all modern biomedical advances, 1/3 of patients remain with an unrecognized cause (idiopathic) of male infertility. At the same time, we must not forget that most patients from this category do not want to resort to assisted reproductive technologies to realize paternity, and they strive to achieve pregnancy in a natural way. Therefore, the search for the causes of male infertility remains an urgent issue in modern urology field. This article reviews the literature on the role of mast cells in the formation of fibrosis in tissues, including the testis. Mast cells affect the proliferation, functioning and phenotype of fibroblasts put under hypoxic conditions. Fibroblast activation enhances collagen fibrillogenesis. Studies by Russian and foreign scientists have shown that with sperm pathology, the number of mast cells in the connective tissue of the testicle increases sharply. Against the background of an increase in the number of mast cells in the interstitium of the testis, fibrosis of the wall of the convoluted seminiferous tubules increases. Moreover, in severe spermatogenesis disorders (Sertoli cell-only syndrome, Germ cell aplasia), mast cells were found both in the peritubular space and in the lumen of the convoluted seminiferous tubules. Most infertile men have significant amounts of significant amounts of mast cells in their ejaculate. There are sporadic data on the negative correlation between the presence of mast cells in seminal plasma and the concentration and motility of spermatozoa. Conclusion. The negative effect of mast cells on spermatogenesis remains unknown to the end. Mast cells have a high ability to migrate to connective tissue, which levels increase during inflammation, and the production of many mediators, proteases and histamine, cytokines, which can be both a trigger in the formation of sperm pathology and the cause of the formation of fibrosis in the testicle.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121064065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolated chemohyperthermal perfusion of the liver with melphalan in the treatment of unresectable liver metastases with uveal melanoma","authors":"A. Kaprin, S. A. Ivanov, V. Unguryan, L. Petrov, E. Kruglov, Y. Pobedintseva, E. V. Filimonov, A. Isaeva","doi":"10.17709/2410-1893-2021-8-4-8","DOIUrl":"https://doi.org/10.17709/2410-1893-2021-8-4-8","url":null,"abstract":"Uveal melanoma belongs to rare malignant neoplasms, and the biological peculiarity of this tumor determines the high rate of distant metastasis, which reaches 60 %. Most frequently, uveal melanoma metastases are localized in the liver and have an isolated character. At the same time, despite the achievements of modern drug therapy, the treatment results of this category of patients remain unsatisfactory. Among the regional methods of treatment of metastatic uveal melanoma, surgical treatment is considered to be the most effective. Median survival rate in the group of radically operated patients (R0) is 27 months. At present, in the vast majority of cases, surgical treatment is impossible because of multiple bilobar metastasis and advanced cancer process. Median life expectancy of patients with liver metastases is only 9 months. A promising method of regional treatment of inoperable metastatic uveal melanoma is isolated liver chemoperfusion. Multidisciplinary team of Radiology Scientific Research Center and Kostroma Oncologic Dispensary for the first time in Russia presents a clinical case of a patient with isolated inoperable uveal melanoma liver metastases using an innovative method - isolated high-dose chemo hyperthermic liver perfusion with melphalan. The article describes in detail the method of the procedure, estimates immediate (partial response in 1 month after the procedure) and long-term results of the method (stabilization of the condition against the background of immunotherapy in 9 months after surgery). Based on the presented clinical observation, isolated liver chemoperfusion with melphalan for this category of patients is reasonable. However, despite the encouraging immediate results, clinical experience needs to be accumulated in order to be further evaluated in clinical trials.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"212 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133258150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human resources in a healthcare institution as the management efficacy improvement factor","authors":"A. V. Kukurika","doi":"10.17709/2410-1893-2021-8-4-11","DOIUrl":"https://doi.org/10.17709/2410-1893-2021-8-4-11","url":null,"abstract":"The article analyzes the current HR of the local healthcare organization. There was carried a SWOT-analysis of personnel management has been out, disclosed the main problems of the management system of a medical institution. Development strategies and an algorithm for improving personnel policy are formulated in order to increase the efficiency of management and the quality of medical care to the population.Purpose of the study. Is to analyze the current personnel policy, SWOT-analysis of personnel management, develop a strategies and set of practical recommendations for improving the management system of a public health institution while ensuring high quality medical services to the population.Materials and methods. An analysis of the personnel policy in a phthisiatric institution (Makeevka Municipal TB Dispensary, Donetsk region) was carried out, the heads of the main structural divisions were questioned. The analysis results are structured using the SWOT scale.Results. Based on the results of the sociological survey, the main advantages and disadvantages of personnel policy were identified, a SWOT analysis matrix was drawn up, and strategies for the development of a budgetary healthcare institution were developed. The breakthrough strategy is based on improving the material and technical base, attracting funding and investment, increasing the speed and quality of TB care at the regional level using information technology, while the survival strategy is based on increasing the income of health workers, social protection, improving working conditions and developing personnel motivation systems. The main recommendations include increasing the number of employees, creating a strategy for the formation of qualified personnel, improving the quality of the working environment, creating a favorable psycho-emotional climate, and developing an effective system of incentive payments.Conclusion. The medical industry is in dire need of modernization in order to improve personnel processes, financial, economic and regulatory support. The conducted research and developed recommendations will have a beneficial effect on the achievement of the main strategic goal - improving the health and quality of life of the population of the state.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125061941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Urolithiasis complicated by chronic obstructive pyelonephritis in patients with concomitant diseases of the cardiovascular system","authors":"R. Royuk, S. Yarovoy, R. A. Khomov","doi":"10.17709/2410-1893-2021-8-4-7","DOIUrl":"https://doi.org/10.17709/2410-1893-2021-8-4-7","url":null,"abstract":"Purpose of the study. To study the features of chronic obstructive pyelonephritis in patients with recurrent nephrolithiasis and concomitant diseases of the cardiovascular system.Materials and methods. The study included a total of 618 patients suffering from recurrent nephrolithiasis complicated by ChOP. The 3 groups were formed: the first (I) of them included 42 patients with ChOP and uncomplicated coronary heart disease (CHD); the second (II)- 38 patients with CHD complicated by chronic heart failure (CHF); III (control) group consisted of 24 respondents with recurrent urolithiasis.Results. The sample was dominated by men (78 %). In total, there were 474 (76.7 %) patients with intact cardiovascular system. Cardiovascular disease (CVD) suffered from 144 (23.3 %) patients, 38 (26.4 %) of whom had CHF of varying severity. Exacerbations of ChOP in patients of group I developed 1.5 times more often (once in 2.8-3 years) than in patients from the control group (on average after 18 months versus 28), and in group II almost annually (1 time in 13 months). In the entire cohort (n = 618), pyelonephritis was transformed into purulent-destructive in 19 (3.1 %) cases; with concomitant CVD, this complication developed in 7 (4.9 % of 144) cases, the remaining 12 (2.5 % of 474) respondents did not suffer from CVD. In group II, the purulent-destructive form occurred in 4 (10.5 %) patients; their share in the general group of patients with CVD was 2.8 % (the differences were significant - p < 0.05). Of the 19 patients with purulent-destructive pyelonephritis on the background of nephrolithiasis, more than a third (7 people - 36.8 %) had concomitant CHD, and of the 7 patients with this terrible complication that developed on the background of concomitant CHD, more than half (4 people - 57.1 %) had CHF of varying severity. The chemical composition of the urine samples was determined using a NICOLET iS10 spectral analyzer (Thermo scientific, USA). The stages of CHF were determined in accordance with the Strazhesco-Vasilenko classification [N. D. Strazhesco, V. H. Vasilenko, 1935]. Statistical data processing was carried out using the SPSS program 19. Absolute and relative indicators were calculated, as well as the exact Fisher criterion, taking into account the Bonferroni correction; the differences were considered reliable at p < 0.05.Conclusion. in patients with recurrent nephrolithiasis and concomitant CVD (especially with complicated CHF), exacerbations of obstructive pyelonephritis develop more often, the inflammatory process often turns into a purulent-destructive form.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124761141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum HIF-1α and TGFβ1 levels depending on the clinical and morphological characteristics of breast cancer and sensitivity of tumor to neoadjuvant chemotherapy","authors":"E. Zubareva, M. Senchukova, E. V. Virich, M. R. Zubarev, M. A. Goncharova","doi":"10.17709/2410-1893-2021-8-4-5","DOIUrl":"https://doi.org/10.17709/2410-1893-2021-8-4-5","url":null,"abstract":"Purpose of the study. To assess the serum levels of HIF-1 α and TGF-β1 in patients with invasive breast cancer (BC) depending on the clinical and morphological characteristics, molecular biological subtypes and the degree of pathological response (PR) of the tumor and metastases in the regional lymph nodes.Materials and methods. 65 patients with newly diagnosed invasive BC, of whom 32 received from 6 to 8 courses of neoadjuvant chemotherapy (neo-A-CT) were included in this pilot study. The levels of TGF-β1 and HIF-1α were determined by enzyme-linked immunosorbent assay. Statistical analysis was performed using the Statistica 12.0 software.Results. It was found that a high level of TGF-[31 was significantly more often observed in HER2-positive and I-IIa stages of luminal A and triple-negative BC (p = 0.01). HIF-1a levels were significantly higher in the presence of distant metastases (p = 0.04) and slightly higher in the presence of microcalcifications on mammograms (p = 0.07).The analysis showed that patients with grade III-IV of PR in tumor were significantly younger (p = 0.047). Grade III-IV of PR in tumor was significantly more often observed in G3 (p = 0.05), in Her2-positive and triple negative BC (p = 0.01), in the absence of perineural (p = 0.002) and lymphovascular invasion (LVI) (p = 0.045), in the presence of lymphoid infiltration (p = 0.011) and microcalcifications in the tumor (p = 0.043), and slightly higher in ductal BC (p = 0.08). No significant correlations were found between the levels of TGF-p1 and HIF-1a and tumor PR (p = 0.6 and p = 0.9, respectively). However, in patients with grade III-IV of PR in regional metastases, the level of TGFb1 was significantly lower than in patients with grade I-II (p = 0.03).Conclusions. Thus, these data indicate the presence of correlations between the levels of HIF-1 α and TGF-β1 in the blood serum and a number of clinical characteristics of BC. The highest levels of HIF-1α are observed in the presence of distant metastases, and the highest levels of TGF-β1 are noted in HER2-positive and I-IIa stages of luminal A and triple-negative breast cancer. Given the presence of significant correlations between the level of TGF-β1 and the degree of PR in regional lymph nodes, its determination may be useful for assessing the sensitivity of metastases to regional lymph nodes to the neo-A-CT.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126901483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}