Research and Practical Medicine Journal最新文献

{"title":"Buccal ureteroplasty options, techniques, long-term results","authors":"A. Volkov, N. Budnik, O. Zuban, I. D. Mustapaev, M. Abdulaev, A. V. Muziev","doi":"10.17709/2410-1893-2022-9-2-7","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-2-7","url":null,"abstract":"Purpose of the study. Surgical treatment of extended strictures and obliterations of the ureter is still a complicated problem of modern urology. The aim of the study was to analyze our own experience of ureteral replacement plastic surgery with buccal graft, i.e buccal ureteroplasty (BU) with its extended strictures/obliterations.Patients and methods. We’ve observed 25 patients who underwent BU. The lower third of the ureter was affected in 3 (12.0 %) cases, the lower third and the mouth of the ureter was affected in 7 people (28.0 %), the middle third in 2 (8.0 %), the upper third in 5 patients (20.0 %), the combined lesion of the upper third of the ureter and the pelvic‑ureteral segment (PUS) was in 8 (32.0 %) patients. All patients underwent repeated operations on a ureter, all revealed a significant comorbid background. The surgery was performed with a tubularized buccal graft in 13 (52.0 %), the onlay technique was applied in 12 (48.0 %). Laparoscopic surgery was performed in 10 (40.0 %) patients, surgical aid was carried out in an open way in 15 (60 %).Results. Severe complications according to the Clavien‑Dindo classification, requiring hospitalization of patients in the intensive care unit with organ dysfunction, as well as lethal outcomes were absent. The follow‑up period of patients ranged from 1 to 57 months (an average of 14.7 months). One patient after laparoscopic BU with a tubular graft had a short (1 mm) stenosis in the anastomosis area for 6 months of follow‑up, which was successfully eliminated by laser endoureterotomy. During the entire follow‑up period, residual hydronephrosis was recorded in 4 patients (16.0 %) against the background of complete patency of the anastomosis. A control flexible ureteroscopy performed in 16 (64.0 %) patients did not reveal rejection of the buccal graft. There are still 20 patients under our supervision.Conclusion. Our experience shows that the implementation of BU is possible on any part of the VMP using various techniques. This operation can be regarded as the \"second\" line of surgical treatment of strictures and obliterations of the ureter, especially after unsuccessful attempts of other reconstructions in a group of patients with severe concomitant pathology.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129354040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of regulatory documents regulating the fight against COVID-19 and other healthcare tools used to manage the COVID-19 pandemic in Kazakhstan","authors":"V. Benberin, B. T. Kaikenov, Y. Samsonov","doi":"10.17709/2410-1893-2022-9-2-14","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-2-14","url":null,"abstract":"Purpose of the study. Explore organizational tools for managing health system resources to fight the COVID‑19 pandemic in Kazakhstan.Materials and methods. The analysis of regulatory documents (5 decrees of the chief sanitary doctors and 2 orders of the Ministry of Health of the Republic of Kazakhstan) regulating measures for the COVID‑19 pandemic was carried out. These legal acts were approved in the first months after the declaration of the state of emergency in the country after the detection of the first cases of coronavirus infection in Kazakhstan. These laws and regulations governed the provisions and measures to combat COVID‑19. And also the analysis of measures for the introduction of quarantine or restrictions based on the monitoring of the R number of the virus was carried out.Results. The article discusses the issues of resource provision to contain the spread of COVID‑19 in the country. The analysis of the regulatory legal acts regarding the types of assistance provided to the population to prevent the spread of infection, as well as the treatment strategy for patients suffering coronavirus infection. These laws regulate the introduction, weakening/strengthening or cancellation of restrictive measures in the regions, mainly related to the activities of organizations and public places where there is a high concentration of the population, as well as the movement of masses of people.Conclusion. The study showed what types of assistance are provided to the population to halt the COVID‑19 pandemic, ranging from tactics at the outpatient level to the deployment of care at the inpatient level. In addition, the monitoring of the R value of the virus can be a working tool for the authorities in the introduction of quarantine measures.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"290 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113992356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of biological activity of 2-quinoline-2-yl-derivative 1,3-tropolone in experiment","authors":"E. Lukbanova, E. Dzhenkova, A. Goncharova, A. Maksimov, E. F. Komarova, V. Minkin, Y. A. Sayapin, E. A. Gusakov, L. Z. Kurbanova, A. Kiblitskaya, E. Zaikina, M. V. Mindar, M. V. Voloshin, A. Shaposhnikov, I. Lysenko, N. Nikolaeva","doi":"10.17709/2410-1893-2022-9-2-4","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-2-4","url":null,"abstract":"Purpose of the study. Was to reveal the antitumor effect of 2‑(6,8‑dimethyl‑5‑nitro‑4‑chloroquinoline‑2‑yl)‑5,6,7‑trichloro‑1,3‑tropolone in subcutaneous PDX models of human lung cancer.Material and methods. The studied tropolone was synthesized using a method of expanding the o‑quinone cycle. Assess to it’s toxic effects was given by the survival and changes in the health status of female Balb/c Nude mice. Antitumor tropolone effects were studied in subcutaneous patient‑derived xenograft (PDX) models of human squamous cell lung cancer in Balb/c Nude mice. The average volumes of tumor nodes and tumor growth inhibition (TGI %) rate were taken into account. Biochemical blood tests and histological analysis of the tumor material were performed in recipient mice.Results. An analysis of acute tropolone toxic effects did not reveal the lethal dose. The maximal TGI was observed on day 36 of the experiment in group 5 which have received 2.75 mg/g tropolone and accounted 73.5 % for females and 74.4 % for males. The average tumor volumes in females of this group were 431.3 ± 1,1 mm3 on day 33 of the experiment, in males – 428.9 ± 1,7 mm3 on day 30, and then the tumor volumes declined. The biochemical analysis of blood and histological examination of the tumor tissue of recipient mice reflect the severity of the antitumor effect on the dose of the studied tropolone.Conclusion. The research demonstrated the antitumor activity of 2‑(6,8‑dimethyl‑5‑nitro‑4‑chloroquinoline‑2‑yl)‑5,6,7‑trichloro‑1,3‑tropolone against subcutaneous PDX models of human NSCLC. The revealed tendencies can be used to search for effective modes of the compound application in clinical practice.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123913034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of apoptosis factors in mitochondria of brain cortex cells in female С57ВL/6 mice in dynamics of B16/F10 melanoma growth combined with comorbidity","authors":"E. Frantsiyants, I. Neskubina, N. Cheryarina, E. Surikova, A. Shikhlyarova, V. Bandovkina, L. Nemashkalova, I. Kaplieva, L. Trepitaki, P. Kachesova","doi":"10.17709/2410-1893-2022-9-2-1","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-2-1","url":null,"abstract":"Purpose of the study. To analyze the apoptosis indicators in mitochondria of brain cortex cells in female С57ВL/6 mice in the dynamics of B16/F10 melanoma growth alone and in combination with comorbidity, i.e. chronic neurogenic pain.Materials and methods. Female С57ВL/6 mice (n = 168) were used in the experiment. Groups accounted: intact group (n = 21); control group (n = 21) with a model of chronic neurogenic pain (CNP); comparison group (n = 63) with B16/F10 melanoma transplanted subcutaneously; main group (CNP + B16/F10) (n = 63). Levels of cytochrome C (ng/mg protein), caspase 9 (ng/mg protein), Bcl‑2 (ng/mg protein), AIF (ng/mg protein), calcium (Ca 2+) (mMol/g protein) were measured by ELISA in mitochondrial samples. Statistical analysis was performed using the Statistica 10.0 program.Results. In a week of the tumor growth in presence of comorbidity, i.e. CNP, levels of calcium in murine brain cortex mitochondria were 1.4 times higher (p < 0.05) than in the comparison group at the same time; in 2 weeks the levels declined by 80.1 times and after 3 weeks declined by 37.7 times. Compared to the values in the comparison group AIF levels in animals with CNP+B16/F10 were lower by 25 and 1.8 times (p < 0.05) at weeks 1 and 3, respectively. Higher levels of Вcl‑2 in the group with CNP + B16/F10 were registered at weeks 2 and 3 by 2 and 1.4 times (p < 0.05), respectively. Levels of cytochrome C were decreased in animals with CNP+B16/F10 at weeks 1–3 by 3.2, 1.5 (p < 0.05) and 2.8 times, respectively. Caspase 9 in CNP+B16/F10 after 3 weeks exceeded the values in the comparison group by 2.6 times.Conclusions. Combination of CNP and melanoma at an early stage in the animal body promotes the accumulation of calcium and suppression of AIF and cytochrome C in mitochondria of the brain cortex. By the terminal stage of tumor growth in presence of comorbidity (CNP), suppression of most units of the respiratory chain of mitochondria of brain cortex cells is formed.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124633269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Possibilities of using design thinking to improve the process of medical care","authors":"A. Popsuyko, Y. Danilchenko, E. A. Batsina, D. V. Kilizhekova, D. Karas, G. V. Artamonova","doi":"10.17709/2410-1893-2022-9-1-12","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-12","url":null,"abstract":"Purpose of the study. To describe the theoretical foundations and practical application of design thinking technology in the activities of a medical organization as a tool for managing interaction with patients to improve the provision of medical care for cardiovascular diseases.Materials and methods. The work was carried out on the basis of the Research Institute for Complex Issues of Cardiovascular Diseases (Kemerovo). When using methods of sociological research (questioning, content analysis). In the period from 2015 to 2020, 43865 patients were treated at the institution, of which 16886 were questioned. The analysis of domestic and foreign literature, as well as regulatory documents was carried out for the period from 2015 to 2020 using the keywords: \"design thinking\", \"Service design\", \"design thinking in medicine.\"Results. The possibility of adapting the technology of design thinking to medical and diagnostic activities in research institutes, namely to the process of assessing patient satisfaction, is shown. Due to the presence of an effective mechanism for managing interaction with patients, in the research institute in the period from 2011 to 2020, there was a decrease in justified complaints (more than 2 times), and the level of patient satisfaction reaches target values.Conclusion. Design Thinking is part of the patient management process as an initial step in assessing patient satisfaction. The described technology can be used by any medical organization.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132159606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of multispiral computed tomography in detecting and the assessment of the degree of damage of esophago-gastric junction cancer","authors":"V. Diomidova, A. Dmitrieva","doi":"10.17709/2410-1893-2022-9-1-6","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-6","url":null,"abstract":"Purpose of the study. To find out the abilities of multispiral computed tomography (MSCT) using intravenous bolus injection in detecting and preoperative esophago-gastric junction cancer staging in order to improve preoperative diagnosis of this pathological condition.Materials and methods. The patients, diagnosed esophago-gastric junction cancer (n1 = 76), esophagus (n2 = 27) and medically fit people’s (n3 = 30) findings of investigating have been analyzed. All patients went through computed tomography on Light Speed VEX Plus and Light Speed RT 16 (\"GE\", The USA). The findings of MSCT have been compared with anatomical data, which were got after histomorphologic study of post-operation and endoscopic recruitment material.Results. Characteristic MSCT signs of the wall of the esophageal-gastric junction (EGJ) were revealed in healthy individuals, with esophagitis and cancer. The thickness of esophagus paries in normal conditions and in case of esophagitis and EGJ cancer is: 5.4 ± 1.01 mm, 10.36 ± 1.85 mm, 22.53 ± 8.19 mm (p < 0.001). The external diameter of the abdominal part of esophagus in the normal condition is 14.2 ± 1.68 mm, in case of esophagitis is 17.96 ± 3.7 mm, EGJ cancer is 27.9 ± 9.48 mm (p < 0.001). The main statistically significant (p < 0.001) qualitative character of esophago-gastric junction canser were: non-visualization of mucic in afflicted areas (96.8 %), bosselated upper and lower contours of narrowing (75.2 %), dissymmetric parries pachymenia (70.4 %), blunt-edged outer contours (58.4 %), cotyloid suprastenotic esophageal distensibility (45.6 %). The delicacy depended on the degree of damage delicacy and was at the stage Т1 – 66.7 %, Т2 – 76.5 %, Т3 – 77.8 %, Т4а – 86.2 %, Т4b –100.0 %, in general, diagnostic accuracy of the method in EGJ cancer Т-staging was 81.6 %.Conclusion. The results of the work showed that the inclusion of MSCT with intravenous bolus contrast in the algorithm of the study of patients with suspected tumor pathology of the EGJ will improve the diagnosis of cancer of the EGJ.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126959373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Gene Expression Omnibus high-throughput sequencing data for the determination of microribonucleic acids in the blood plasma of patients with glioblastomas","authors":"A. Pushkin, D. Gvaldin, N. Timoshkina, E. Rostorguev, L. Vladimirova, E. A. Dzenkova","doi":"10.17709/2410-1893-2022-9-1-5","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-5","url":null,"abstract":"Purpose of the study. This work is devoted to the study of blood plasma miRNA patterns in blood plasma using high-throughput sequencing of the Omnibus Gene Expression base and the search for candidate miRNA molecules for the development of a minimally invasive diagnostic panel.Materials and methods. Basing on the open dataset of Omnibus Expression of the NCBI GSE150956 Gene, groups of samples with glioblastoma and conventionally healthy donors were formed. For each sample, information on the levels of miRNA expression was extracted. Determination of significant miRNAs using machine learning algorithms of the R 4.0.4 project. For significant miRNAs, target genes have been performed, an analysis of the improvement of functional characteristics and interactome analysis of target genes of miRNA were performed.Results. The study analyzed the data of 131 samples, where 35 samples with glioblastoma and 96 samples of the conditionally healthy group. Differential expression data were obtained for 945 miRNA. Two panels were obtained using machine learning methods, common miRNA – hsa-miR 3180, hsa-miR 3180-3p, hsa-miR 6782-5p, hsa-miR 182-5p, hsa-miR 133b and hsa-miR 670-3p. For significant miRNAs, information was obtained on experimentally confirmed target genes, a gene ontology demonstrating their participation in enzyme binding, participation in the regulation of primary cellular metabolic processes, and the development of glioblastomas and cancer in general.Conclusion. As a result of layer-by-layer filtering and application of machine learning algorithms, significant miRNAs were identified that are candidates for a diagnostic panel of a minimally invasive method of high-grade glial tumors.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126535356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lung cancers biomarkers","authors":"D. Kharagezov, Y. Lazutin, E. Mirzoyan, A. Milakin, O. Stateshny, I. A. Leiman, K. D. Iozefi","doi":"10.17709/2410-1893-2022-9-1-9","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-9","url":null,"abstract":"More than 1.8 million of new cases of lung cancer (LC) are registered each year worldwide. LC is the leading cause of cancer death in both developing and developed countries, and the 5 years survival rate is as low as 19 %. Many factors explain such unsatisfactory outcomes, including the LC diagnosis at an advanced stage, when the currently available treatments can rarely provide cure. Biomarkers are used to assess the development risks, screening, diagnosis, monitoring, and prognosis, and to personalize the LC treatment. Clinical use of biomarkers is essential for the identification of a high-risk group for screening for LC and differentiating early LC from benign pulmonary lesions. Current trends in the development of LC biomarkers involve the integration of molecular biomarkers with clinical and radiological characteristics using artificial intelligence for the development of imaging biomarkers, and using highly sensitive technologies such as next-generation sequencing for molecular research. LC biomarkers are now at all stages of development, from discovery to clinical trials requiring high-quality clinical validation. Reliable biomarkers are especially needed to differentiate malignant and benign lesions in the lung tissue and to identify those at greatest risk of developing lung cancer. Scientific advances in understanding LC have led to the development of biomarkers that demonstrate sufficient accuracy in clinical validation studies. Promising trends in the development of LC biomarkers include highly sensitive and increasingly accessible NGS and radiomics technologies, along with the use of easily collected biomaterials, which in combination with other tumor characteristics contribute to the development of biomarkers for assessing the LC development risks, diagnosis, monitoring, prognosis and personalized therapy. This review focuses on the development, current application, and future trends in the use of LC biomarkers.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130711403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytopathological characteristics for provoked lesions of squamous epithelia of digestive system in animal models","authors":"V. Shestakova, L. Nigmatullina, I. Atadzhanov, S. Donskov, Yu. Kozlovskaya, I. Dolgopolov, M. Rykov","doi":"10.17709/2410-1893-2022-9-1-4","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-4","url":null,"abstract":"Purpose of the study. Investigation of morphological characteristics of changes in the epithelium of foregut in experimental animals put under conditions of provoked carcinogenesis.Materials and methods. The method of chronic experiment on animals is applied: 40 female non-linear white rats, which are divided into 4 equal groups (n = 10). The first (I) and second (II) control groups of animals were exposed to mechanical trauma of the oral mucosa with additional application of 0.9 % NaCl solution and 1 % aqueous dimethyl sulfoxide (DMSO) solution with a frequency of 2 times a week. The third group of animals, i.e. the main group (4-NQO), was subjected to mechanical traumatization of the oral mucosa with additional application of 1 % DMSO aqueous solution containing 0.1 mg/ml of 4 nitroquinoline-N-oxide, with a frequency of 2 times a week. The fourth group of animals was intact. On the 1st, 7th, 10th, 14th day from the beginning of the experiment, and then every 14 days, cytological material was taken from the oral mucosa and stained with Papanicolaou stain. To compare the emerging changes, the histological picture of the organs of the foregut was studied.Results. Animals from the main group (4-NQO) showed a lag in body weight gain over the standard variance in the control (I and II) and intact groups. Atypical cells of indeterminate significance appeared in smears starting from 42 days of the experiment. Starting from the 56th day of the experiment, atypical cells (1–2 in the field of view), described in Bethesda terms as low-grade intraepithelial lesions, were detected in smears obtained from the main group of animals (4-NQO). Parabasal cells of the squamous epithelium with atypical large (more than 3 times compared to the reference) nuclei, anisonucleosis, with variable contours of the nuclear membrane within one cell, coarse chromatin were recognized as diagnostically significant.Conclusion. The severity of morphological changes in foregut epithelium in the direction of the precancerous state is significantly higher in the main group with prolonged exposure to carcinogenic factor, compared with the control and intact groups (р < 0.05). Thus, the effectiveness of modeling the conditions of provocation of carcinogenesis of the epithelium of the upper digestive tube in experimental animals was studied and proved by mechanical traumatization of the oral mucosa with additional application of carcinogen (4-NQO). This model of provocation of carcinogenesis will be used in the next study of neoplastic processes of the female reproductive system.","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131204663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in levels of urokinase receptor and other components of fibrinolytic system in brain tissues in urokinase gene-knockout mice with B16/F10 melanoma growing together with chronic neurogenic pain","authors":"E. Frantsiyants, V. Bandovkina, I. Kaplieva, N. Cheryarina, E. Surikova, I. Neskubina, Y. Pogorelova, L. Nemashkalova","doi":"10.17709/2410-1893-2022-9-1-1","DOIUrl":"https://doi.org/10.17709/2410-1893-2022-9-1-1","url":null,"abstract":"Purpose of the study. An analysis of the changes in components of the urokinase system in the brain of urokinase gene-knockout mice (uPA-/-) with B16/F10 melanoma growing alone and together with chronic neurogenic pain (CNP).Materials and methods. The study included male and female C57BL/6-PlautmI.IBug-ThisPlau6FDhu/GFDhu mice (uPA-/-) (n = 48) and C57BL/6 mice (uPA+/+) (n = 80) with transplanted B16/F10 melanoma growing solitarily and together with CNP. Levels of the urokinase receptor (uPAR) and plasmin (PAP) and activity and levels of the PAI-I inhibitor were measured in the brain of animals by ELISA.Results. Levels of uPAR, PAI-I and PAP in the brain differed only in intact uPA-/- males, being on average 1.6 times higher (p < 0.05) than in uPA+/+ mice. Among animals with CNP, uPA-/- males showed increased PAI-I by 1.3 times (p < 0.05) and decreased PAP by 2.6 times (p < 0.05), while in uPA+/+ males, changes in PAI-I and PAP were opposite; in uPA-/- females, levels of all indicators increased by 1.6–2.1 times (p < 0.05), unlike uPA+/+ females. Among animals with melanoma only, changes in the levels of uPAR, PAI-I and PAP in the brain tissues in uPA-/- males differed from the group with CNP and from uPA+/+ males; in uPA+/+ females, levels of uPAR and PAP increased by 1.7 and 3.0 times (p < 0.05), and only PAP increased in uPA-/- females by 3.2 times (p < 0.05). Combination of CNP with melanoma in uPA-/- mice, regardless of their gender, down-regulated levels of uPAR and PAI-I on the average by 1.5 and 2.0 times, respectively (p < 0.05), and up-regulated PAP on the average by 2.2 times (p < 0.05) compared to the levels in animals with CNP; in uPA+/+ animals, similar decline of uPAR by 3.7 times (p < 0.05) was registered only in males, and an increase of PAI-I by 2.0 times (p < 0.05) was noted in all mice.Conclusion. Changes in the studied parameters in the brain tissue of urokinase gene-knockout animals in response to stress factors indicate the role of the brain urokinase system in the response to both CNP and melanoma growth, and the gender specificity of these changes may be another factor that conditions gender differences in the risk of occurrence and course of cutaneous melanoma. ","PeriodicalId":334809,"journal":{"name":"Research and Practical Medicine Journal","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122884472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}