{"title":"COMUNIDAD DE HELMINTOS DE POLLOS CASEROS (GALLUS GALLUS DOMESTICUS LINNAEUS, 1758) EN SEROPÉDICA, RIO DE JANEIRO, BRASIL","authors":"R. O. Simões, Valéria da Silva Carneiro, J. Luque","doi":"10.24039/RNH20211521196","DOIUrl":"https://doi.org/10.24039/RNH20211521196","url":null,"abstract":"The domestic chicken, Gallus gallus domesticus Linnaeus, 1758, is an important component of the agribusiness segment, and Brazil is one of the world’s largest broiler producers and exporters. The present study aimed to characterize the composition and structure of the helminth community of backyard chickens, G. g. domesticus, in Seropedica, Rio de Janeiro, Brazil. Fifty-five adult chickens were studied. The overall helminth species richness was 12. The nematodes Capillaria sp. and Heterakis gallinarum (Schrank, 1788), recovered from small intestine and cecum, respectively, presented the highest prevalence and mean abundance and were considered central species. In addition, these species presented the highest values of frequency of dominance and Berger. The pairs of helminth species Amoebotaenia cuneata (von Linstow, 1872) – Raillietina tetragona (Molin, 1958); Davainea proglottina (Davaine, 1860) – A. cuneata; and H. gallinarum – Capillaria sp. showed significant positive correlation between their abundance and prevalence. Gongylonema ingluvicola Ransom, 1904 and H. gallinarum showed significant correlation between host sex and helminth abundance, while there was no correlation between host sex and helminths prevalence. The knowledge of helminth community structure in free-range chickens is important to adopt better measures for control and prevention of helminth infections.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125406259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bruna Cavaco, L. Carvalho, Lais Modolo Conti, Max Rondon Werneck
{"title":"RELATO DE CHARAXICEPHALOIDES POLYORCHIS GROSCHAFT & TENORA 1978 (DIGENEA: PRONOCEPHALIDAE) DE UNA TORTUGA VERDE- CHELONIA MYDAS (LINNAEUS, 1758) EN BRASIL","authors":"Bruna Cavaco, L. Carvalho, Lais Modolo Conti, Max Rondon Werneck","doi":"10.24039/RNH20211521199","DOIUrl":"https://doi.org/10.24039/RNH20211521199","url":null,"abstract":"This note paper report the occurrence of Charaxicephaloides polyorchis Groschaft &Tenora 1978 collected from a green sea turtle - Chelonia mydas (Linnaeus, 1758) from Brazil. Our specimen is larger than the original description and the following descriptions, providing a new information on this species. This represents the first record with morphometric data of C. polyorchis in a green turtle from Brazil and the third record with morphometric data of this trematode.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134416671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alberto Frango-Ramos, R. Delgado, Emily Catalano-Donaire, Jhon Jesus Arzapalo, Renzo Nino Incani-Cotognini, M. Perteguer-Prieto, Elizabeth Ferrer-Jesús
{"title":"ESTANDARIZACIÓN DE LA TÉCNICA DE PCR PARA LA DETECCIÓN DE LA SECUENCIA ITS1 DE NECATOR AMERICANUS (STILES, 1902) Y CLONACIÓN DEL PRODUCTO PARA SU USO COMO CONTROL","authors":"Alberto Frango-Ramos, R. Delgado, Emily Catalano-Donaire, Jhon Jesus Arzapalo, Renzo Nino Incani-Cotognini, M. Perteguer-Prieto, Elizabeth Ferrer-Jesús","doi":"10.24039/RNH20211521197","DOIUrl":"https://doi.org/10.24039/RNH20211521197","url":null,"abstract":"Hookworm is generally caused by Necator americanus (Stiles, 1902), causing digestive symptoms and anemia. The diagnosis by coprology can have low sensitivity, especially in light parasite loads. The Polymerase Chain Reaction (PCR) is a sensitive and specific technique, which must be adapted to laboratory conditions and positive controls are necessary. The objective of this work was the standardization of the PCR technique for the amplification of the ITS-1 sequence of N. americanus in stool samples and its cloning for its use as a control. Three DNA extraction protocols were standardized (Phenol/ chloroform, Saline Precipitation, and Chelex® 100 Resin). Optimal reagent concentrations (MgCl2, BSA, dNTP, primers, and Taq polymerase) were determined, as well as the hybridization temperature and number of cycles. The analytical sensitivity and specificity of the technique were determined. For cloning, the ITS-1 sequence amplified by PCR was purified and ligated with the vector pGEM-T-Easy. Competent E. coli XL1Blue MRF` cells were transformed with the ligation mixture (pGEM-T-easy-Na-ITS1), recombinant colonies were identified and plasmid DNA was extracted from them. The best DNA extraction protocol was phenol / chloroform, the optimal conditions for PCR were; 1.5 mM MgCl2, 0.5 mg/mL BSA, 100 µM dNTP, 0.6 µM primers, and 1 U Taq polymerase, 56 °C hybridization temperature and 40 cycles. The optimal amounts of reagents were less than the amounts used by other authors, allowing saving of reagents. 100% specificity and an analytical sensitivity of 10 pg of DNA (extracted from stool samples), and 10 fg of the plasmid (with the cloned sequence) were obtained, which worked very well as positive control.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115240620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ACTUALIZACIÓN EN FASCIOLOSIS HUMANA EN EL PERÚ: DIAGNÓSTICO, TRATAMIENTO Y PROPUESTA DE CLASIFICACIÓN CLÍNICA","authors":"L. Marcos, Angélica Terashima","doi":"10.24039/rnh2007121156","DOIUrl":"https://doi.org/10.24039/rnh2007121156","url":null,"abstract":"La infección humana por Fasciola hepatica en el Perú es una enfermedad infecciosa emergente. En estarevisión describimos la situación de la fasciolosis en el Perú, en base a los estudios mas recientes sobreepidemiología, métodos de diagnóstico y tratamiento. Proponemos una nueva clasificación clínica en baseal estado de la enfermedad. Estudios recientes han destacado la variabilidad clínica de esta infección quepuede comprender desde una infección indolente a una severa que puede comprometer la vida del paciente. Un incremento en el número de casos humanos ha sido reportado en todo el mundo, y en especial en la Región Andina de Latinoamérica. Las manifestaciones clínicas más comunes en la fase aguda son: hepatomegalia, fiebre, pérdida de peso y eosinofilia. En la fase crónica, esta puede ser leve e inespecífica, pero también severa como obstrucción biliar, colangitis, quistes calcificados hepáticos, cálculos vesiculares y fibrosis hepática. La Técnica de Sedimentación Rápida descrita por Lumbreras debe ser aplicada para el diagnóstico de la fase crónica y para estudios epidemiológicos en zonas endémicas. El examen directo solo detecta 2 % de los casos. El nuevo examen diagnostico ELISA Fas2 (catepsina L1) tiene una sensibilidad del 92,4 % y especificidad del 83,6 %, y esto fue observado en 634 sujetos peruanos en áreas endémicas y es útil tanto para la fase aguda como la crónica. Las anormalidades radiológicas más comunes en la fase aguda son: lesiones hipodensas hepáticas en forma de “camino”, abscesos hepáticos y hematoma subcapsular. La ecografía abdominal tiene una baja sensibilidad en casos crónicos y no es recomendada para tamizaje. El triclabendazol es el tratamiento de elección con una dosis única (tasa de curación ≥ 90 %) para ambas fases, pero casos de resistencia a la droga es ahora una preocupación aunque solo en animales. El nuevo arsenal de información disponible puede ser aplicado a los programas de prevención y control en el Perú.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"174 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122041055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NEOTROPICAL HELMINTHOLOGY: DESAFÍOS Y OPORTUNIDADES DE UNA REVISTA ELECTRÓNICA ARBITRADA","authors":"Rufino Cabrera","doi":"10.24039/rnh2007111146","DOIUrl":"https://doi.org/10.24039/rnh2007111146","url":null,"abstract":"La Asociación Peruana de Helmintología e Invertebrados Afines (APHIA), fundada en la ciudad de Lima, Perú, el 12 de febrero de 2005, tiene como fin incentivar y desarrollar el estudio de los helmintos e invertebrados afines con énfasis en la conservación, biodiversidad, sanidad vegetal, salud humana y animal.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122300513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EQUINOCOCOSIS QUÍSTICA COMO UN PROBLEMA DE SALUD PÚBLICA EN AMÉRICA LATINA Y NUEVOS ENFOQUES PARA SU CONTROL","authors":"Ciro Ferreira, Pilar Irabedra","doi":"10.24039/rnh2007121153","DOIUrl":"https://doi.org/10.24039/rnh2007121153","url":null,"abstract":"Cystic echinococcosis is a zoonosis caused by a cestode, Echinococcus granulosus, of the Taeniidae family. The adult form of the tapeworm affects canids, particularly dogs and foxes, while the larva is harbored by sheep, cattle, goats and other mammals.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128120354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abelardo Morales B, Gerardo Campos A, David Fernández, Francisco García, V. Bermúdez
{"title":"CARCINOMA EPIDERMOIDE ASOCIADO A LA INFESTACIÓN CRÓNICA POR HABRONEMA MUSCAE EN UN CABALLO DE RAZA PINTO: REGISTRO DE UN CASO EN VENEZUELA","authors":"Abelardo Morales B, Gerardo Campos A, David Fernández, Francisco García, V. Bermúdez","doi":"10.24039/rnh2008221139","DOIUrl":"https://doi.org/10.24039/rnh2008221139","url":null,"abstract":"Se remite un caballo (Equus caballus), sexo macho, de raza Pinto y 8 años de edad. Con historia de balanopostitis nodular exudativa crónica complicada con infección bacteriana secundaria data de 4 años. Emaciación crónica severa. Oliguria, estranguria y polidipsia. Linfadenomegalia de nódulos linfáticos inguinales y femurales preescapulares. Se le practicó eutanasia. Posteriormente se le prácticó necropsia por las técnicas sistemáticas descritas para equinos. Se tomaron secciones de tejido para estudio histopatológico y fueron procesadas por los métodos convencionales de procesamiento histológico. Fueron tomadas muestras de heces para estudios parasitológicos. El examen macroscópico reveló emaciación crónica severa. Balanopostitis exudativa crónica severa con infección bacteriana secundaria. Se evidencio hidroperitoneo marcado. Linfadenomegalia de nódulos mesentéricos. Los cortes histológicos evidenciaron: Balanopostitis crónica severa, con focos de proliferación de células escamosas espinocelulares con abundante acantosis, pleomorfismo, hipercromasia nuclear, anaplasia, figuras mitóticas típicas y atípicas constantes, presencia de perlas corneas abundantes. En algunas zonas se observaron granulomas con formas parasitarias tipo Habronema. Los resultados del estudio coprológico fueron consistentes con huevos de Habronema muscae. En conclusión: la historia clínica, los hallazgos macroscópicos e histológicos evidencian un síndrome de balanopostitis crónica y carcinoma epidermoide metastásico.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132798242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"NEW APPROACH ABOUT NEOTROPICAL HELMINTHOLOGY","authors":"J. Cárdenas-Callirgos, J. I. Oliver","doi":"10.24039/rnh2008221135","DOIUrl":"https://doi.org/10.24039/rnh2008221135","url":null,"abstract":"Helminths are a group of invertebrates of major scientific interest due to their high impact on public health and the diverse evolutionary histories that some of its members have followed in order to adapt to the parasitic lifestyle. In the Neotropical region, though, helminth research has been traditionally associated with their effect on public health. This topic awaits more reflection and political support for its beneficial implementation, especially in rural zones where the prevalence of infection by helminth parasites in humans can be very high (Cabrera, 2003).","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128434004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"DISTRIBUCIÓN GEOGRÁFICA Y HOSPEDEROS DE ANCYLOSTOMA PLURIDENTATUM (ALESSANDRINI, 1905) (NEMATODA, ANCYLOSTOMATIDAE) EN LAS AMÉRICAS","authors":"Dely Noronha, Magda Sanches, Roberto Magalhães Pinto","doi":"10.24039/rnh2008211132","DOIUrl":"https://doi.org/10.24039/rnh2008211132","url":null,"abstract":"Hasta el momento no hay registros de la historia de Ancylostoma pluridentatum que fue descripta en Italia con base en especimenes brasileños en 1905. Solo dos referencias autóctonas son disponibles, una de 1910 y otra de 2008, sin datos históricos durante los 98 años en que la especie ha sido ignorada en Brasil. En la presente nota la ocurrencia de A. pluridentatum en las Américas es compendiada con brevedad en lo que se refiere a su distribución geográfica y huéspedes. En América del Sur, A. pluridentatum es citada en las regiones Sudeste (Mata Atlántica) y Norte (Floresta Amazónica) de Brasil, en Argentina (provincia de Formosa) y en Colombia (Choco, Nariño); en América Central es referida en Panamá (Achiote, Colon) y en América del Norte en Norte, Sudeste y Sur de Florida.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124676430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Brandão, Salvatore Siciliano, Marcia Chame, R. Machado, J. L. Luque
{"title":"HUEVOS DE TRICHINELLOIDE (NEMATODA) EN UN PETREL GIGANTE (MACRONECTES GIGANTEUS, PROCELLARIFORMES: PROCELLARIIDAE) DEL SUR DE BRASIL","authors":"M. Brandão, Salvatore Siciliano, Marcia Chame, R. Machado, J. L. Luque","doi":"10.24039/rnh2008211131","DOIUrl":"https://doi.org/10.24039/rnh2008211131","url":null,"abstract":"Durante el invierno de 2003 un ejemplar del petrel gigante (Macronectes giganteus) fue encontrado en la playa Atlântida Sul, en la costa norte del Estado de Rio Grande do Sul, Brasil. Muestras fecales frescas fueron colectadas en el centro de rehabilitación del CECLIMAR (Centro de Estudos Costeiros, Limnológicose Marinhos da Universidade Federal do Rio Grande do Sul) y mantenidas en solución de Raillet &Henry. Usando una técnica de sedimentación espontánea de los huevos de helmintos fueron separadas 10submuestras. Todos los huevos encontrados pertenecieron a la superfamilia Trichinelloidea (n=82). Estoshuevos constituyen un nuevo registro de helminto parásito del pretel gigante.","PeriodicalId":328487,"journal":{"name":"Neotropical helminthology","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133311146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}