Turkiye Tarimsal Arastirmalar Dergisi最新文献

{"title":"Alleviation of Salt Stress with Chitosan Foliar Application and Its Effects on Growth and Development in Tomato (Solanum lycopersicum L.)","authors":"N. Özkurt, Y. Bektas","doi":"10.19159/tutad.1168393","DOIUrl":"https://doi.org/10.19159/tutad.1168393","url":null,"abstract":"Environmental and climatic fluctuations as well as abiotic stress factors affect agricultural production and cause a loss in quality and yield. It is important to find alternative solutions for the sustainability of agricultural production to feed the increasing population. Salt stress is one of the most devastating abiotic stress factors and tomato production is also affected by salt stress since it needs extensive irrigation for high yield. The exogenous application of some plant inducers showed promising results in the induction and improvement of plant tolerance to stress factors. Chitosan (2-amino-2-deoxy-b-D-glucosamine), one of the organic compounds, is getting significant attention in agriculture with its potential. Here, we evaluated the potential of chitosan application for salt stress tolerance on tomato. 0.03% and 0.05% chitosan solutions were applied as a foliar spray to the plant and salt tolerance improvement were investigated under untreated (0 mM NaCl) and 100 mM NaCl conditions. The growth-related (root and shoot diameters, above and below-ground biomass, number of leaves and branches, and plant height), photosynthetic parameters (chlorophyll a, b, total carotenoid content), and ion leakage were investigated. According to the results, chitosan application improves plant development in both untreated and salt-stress conditions and improved plant growth. Also, photosynthetic parameters showed that the application of chitosan increased chlorophyll contents under untreated conditions. Our result suggests that the application of chitosan may have a promising effect on salt stress tolerance and further research may shed light on its molecular mechanisms.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48786864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Purification of Xanthine Oxidase and Investigation of Its Immobilization with Glutaraldehyde","authors":"Yeşim Kaya, S. Işık, S. Uzunoğlu, M. Kaya","doi":"10.19159/tutad.1084383","DOIUrl":"https://doi.org/10.19159/tutad.1084383","url":null,"abstract":"Xanthine oxidase (XO), in purine metabolism is a flavoprotein containing molybdenum with a key role. It has biological functions such as regeneration of NAD+, iron absorption and mobilization, reduction of nitrates. In this study, xanthine oxidase enzyme was purified by Sepharose-4B-L-tyrosine-4-aminobenzamidine dihydrochloride gel according to affinity chromatography technique and immobilization on glutaraldehyde was investigated. XO purified by ammonium sulfate precipitation and affinity chromatography was obtained with an 11.5 % yield and 694.04 degrees of purity. The purity of XO was confirmed by SDS-PAGE and a single band of around 150 kDa was observed. Kinetic constants (KM and VMax) of the enzyme were determined 1.67x10-4 M and 0.56 U/mL.min respectively by using xanthine compound as a substrate. The in vitro effects of NH4F, NH4Cl, CaCl2, ZnCl2, HgCl2, Hg(NO3)2.H2O compounds and commercially named colchicum dispert, commonly used in the treatment of gout disease in the clinic, were investigated. The IC50 values of compounds showing inhibition effect were determined. Afterward XO was immobilized on glutaraldehyde, which was used as a solid support material. The highest XO activity was observed in the sample of the immobilized enzyme at a rate of 6 % glutaraldehyde. The kinetic constants (KM and VMax) of the immobilized enzyme were determined as 5.18x10-4 M and 0.73 U/mL.min respectively. These values revealed that the catalytic activity of the free enzyme was higher than the immobilized enzyme.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67783362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Farklı Gibberellik Asit (GA3) Uygulamalarının Farklı Tuz Yoğunluklarında Sorgum Bitkisinin (Sorghum Bicolor (L.) Moench) Çimlenme ve Fide Gelişimi Üzerine Etkileri","authors":"Cebrail Yildirim, Merve Başak, Bilal Aydinoğlu","doi":"10.19159/tutad.1128902","DOIUrl":"https://doi.org/10.19159/tutad.1128902","url":null,"abstract":"Bu çalışma, sorgum tohumlarının GA3 ile muamale edilmesinin tuzlu koşullarda çimlenme ve fide gelişimine etkilerini belirlemek amacıyla çimlendirme dolabında, petri kapları içerisinde yürütülmüştür. Çalışmada, tohumlar 4 farklı GA3 dozunda (saf su, 100, 200, 300 ppm GA3) 24 saat bekletilmişler ve daha sonra 5 farklı tuz dozunda (saf su, 2500, 5000, 7500, 10000 ppm NaCl) çimlendirilmiştir. Çalışma bölünmüş parseller deneme deseninde 3 tekrarlamalı olarak kurulmuştur. Çalışmada, çimlenme oranı, sürgün uzunluğu, kök uzunluğu, sürgün ve kök yaş ağırlıkları ile sürgün ve kök kuru ağırlıkları incelenmiştir. Tuz ve GA3 uygulamalarının çimlenme oranı dışında incelenen diğer tüm özellikler üzerindeki etkileri istatistiki açıdan önemli bulunmuştur. Tuz*GA3 interaksiyon etkisi önemsiz bulunmakla beraber yapılan çoklu karşılaştırmalarda (Tukey) ortalamalar arasında önemli farklar ortaya çıkmıştır. Artan tuz dozunun çimlenme oranı dışındaki tüm özellikleri olumsuz etkilediği belirlenmiştir. GA3 uygulamalarıda, 200 ve 300 ppm GA3 dozlarının sürgün uzunluğunda tuzluluğun neden olduğu zararı hafiflettiği, ancak diğer özelliklerde bu etkiyi göstermediği tespit edilmiştir.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Şanlıurfa Şartlarında Farklı Sorgum (Sorghum Bicolor var. saccharatum (L.) Mohlenbr.) Genotiplerinin Posasından Yapılan Peletlerin Bazı Fiziksel Özelliklerinin Belirlenmesi","authors":"Mahmut Dok, Mine Aksoy, Ayşegül Çeli̇k, C. Yücel","doi":"10.19159/tutad.1127371","DOIUrl":"https://doi.org/10.19159/tutad.1127371","url":null,"abstract":"Bu çalışmada, Şanlıurfa ekolojik şartlarında 2. ürün olarak yetiştirilen tatlı sorgum (Sorghum Bicolor var. saccharatum (L.) Mohlenbr.) genotiplerinin saplarının özsuyu alındıktan sonraya geriye kalan küspesinden elde edilen peletlerin bazı fiziksel özellikleri saptanmıştır. Çalışmada, hasat edilen bitkilerin sapları sıkılarak suyu alındıktan sonra geriye kalan küspe kurutularak pelet yapılmıştır. Elde edilen peletlerde, pelet kalitesini etkileyen nem içeriği, dayanıklılık direnci, yığın yoğunluğu, parça yoğunluğu, sertlik ve nem alma direnci gibi fiziksel özellikler belirlenmiştir. Çalışmanın iki yıllık ortalama sonuçlarına göre, nem içeriğinin % 3.44-9.48, dayanıklılık direncinin % 94.6-98.9, yığın yoğunluğunun 488-724 kg m-3, parça yoğunluğunun 1039-1305 kg m-3 ve nem alma direncinin % 9.98-16.60 değerleri arasında değiştiği belirlenmiştir. Sertlik direnci ise, genotiplere göre farklılık göstermiş olup 2016 yılında ortalama 3770 N, 2017 yılında ise ortalama 1908 N olarak belirlenmiştir. Elde edilen verilere bakıldığında, tatlı sorgum küspesinden yapılan peletler, ortam koşullarından etkilenmeyen, taşınması ve depolaması kolay yenilenebilir bir enerji kaynağı olarak değerlendirilebilir.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Warming beehives with solar energy stored in water","authors":"M. AL-RAJHİ","doi":"10.19159/tutad.1126564","DOIUrl":"https://doi.org/10.19159/tutad.1126564","url":null,"abstract":"This study presents the possibility of utilizing a passive solar system in sunny places, which collects solar energy by using polyethylene sheet to trap the long-wave thermal radiation and store it in water for warming the internal environment of beehives and its effect on nest temperatures, honey area, pollen area, sealed brood area, and the number of occupied frames. A number of six Langstroth hives containing honeybee colonies of equal strength from the species of hybrid Carniolan are divided into two groups as follows: a control group (untreated hive), and modified beehives that have been treated with the solar energy storage system. The solar energy storage system consists of an insulated wooden drawer located under the beehive's bottom board, containing sealed water bags, and is covered with polyethylene sheet. Solar energy stored in water is used to reduce the variation of inside air temperatures between daylight and night time. The highest values of the honey area, pollen area, sealed brood area, and the number of occupied frames (916 cm2, 842 cm2, 3688 cm2, and 9 frames, respectively) were shown for the modified beehives at the end of March, while the lowest values (98 cm2, 219 cm2, 911 cm2, and 3 frames, respectively) were recorded for the control groups of beehives at January, respectively. In modified beehives treated with a solar energy storage system, there was a significant rise in hive temperature, honey area, pollen area, sealed brood area, and bee population. So, it is recommended to use the new modification for warming beehives.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of Turkish Common Bean Varieties for Resistance Sources to Angular Leaf Spot Disease","authors":"G. Palacıoğlu, G. Özer, M. Yeken, E. Ören, V. Çiftçi, H. Bayraktar","doi":"10.19159/tutad.1126620","DOIUrl":"https://doi.org/10.19159/tutad.1126620","url":null,"abstract":"Angular leaf spot caused by Pseudocercospora griseola is a common disease in all common bean production areas of the world. The breeding and use of resistant varieties are effective and sustainable methods of combating this pathogen. In this context, different resistance genes against the pathogen were explored, and many molecular markers associated with these genes were widely used in breeding studies. This research investigated resistance sources to angular leaf spot on 64 bean varieties widely grown in Turkey using SN02, SH13, and g2303 molecular markers. The findings revealed that the most common gene among the varieties was Phg-2, followed by Phg-ON and Phg-1 genes, respectively. Concerning different gene combinations, it was determined that 17 cultivars had 1 gene, 41 cultivars had 2 genes, and 5 cultivars (Yalova-17, Boncuk, Aslan, Helda, Akman-98) had all three resistance genes. The determined resistance genes may contribute to the breeding studies to be carried out on angular leaf spot.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48872104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fasulyede Tuz ve Kuraklık Stresi Altında PIF Gen Ailesinin Genom Çapında Analizi ve Karakterizasyonu","authors":"Ahmed Sidar Aygören, Recep Aydinyurt, Sümeyra Uçar, Ayşe Gül Kasapoğlu, E. Yaprak, Burak Öner, Selman Muslu, Murat Isiyel, Emre Ilhan, M. Aydın, M. Turan","doi":"10.19159/tutad.1109558","DOIUrl":"https://doi.org/10.19159/tutad.1109558","url":null,"abstract":"Plant growth and development are regulated by light, which is a significant environmental component. It is involved in seedling de-etiolation, phototropism, shadow escaping, seed germination, circadian rhythms, and blooming timing, among other reactions in the plant life cycle (collectively termed photomorphogenesis). These light responses are controlled by phytochromes, which interact with a variety of partner proteins. The purpose of this study was to identify and describe members of the phytochrome-interacting factors (PIFs) gene family including the basic helix loop helix (bHLH) binding site in Phaseolus vulgaris plants, as well as to investigate their responses to salt and drought stress. Various tools in silico approaches were used to identify five Pvul-PIF gene families in the P. vulgaris genome. This gene family contained 324 to 726 amino acids and has molecular weights ranging from 35.11 kDa to 77.67 kDa. The theoretical isoelectric points range from 6.03 (Pvul-PIF-3.3) to 8.30 (Pvul-PIF-3.2). Pvul-PIF proteins were shown to be clustered in three main groups with Arabidopsis thaliana, Populus trichocarpa, Solanum lycopersicum, Zea mays, Arachis hypogaea L., Oryza sativa, Vitis vinifera, Glycine max, and Phaseolus vulgaris species as a result of the phylogenetic study. Segmental duplication was detected between Pvul-PIF-3.2, Pvul-PIF-3.3 and Pvul-PIF-3.1 genes, Pvul-PIF-4.1 and Pvul-PIF-4.2 genes and Pvul-PIF-3.3 and Pvul-PIF-3.1 genes. When the expression patterns of the Pvul-PIF genes were examined, it was observed that they had different levels of expression under salt and drought stress and that they may be involved in specific biological and molecular processes in response to different abiotic and biotic stresses. The results of this research, which were established for the first time in the response to salt and drought stress in P. vulgaris of the PIF gene family, will be a valuable source of knowledge and additional information in the fields of plant biotechnology, agricultural biotechnology, and molecular biology.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel viewpoint by headspace/GC–MS: Volatile Oil of Lavandula angustifolia origin of Denizli Provience","authors":"Oğuzhan Özdemir, Nurten Yilmaz, Mesut Gok, M. Kaya","doi":"10.19159/tutad.1099620","DOIUrl":"https://doi.org/10.19159/tutad.1099620","url":null,"abstract":"This study aims to evaluate the volatile oil components by headspace gas chromatography/mass spectroscopy (headspace/GC-MS) analysis, the antimicrobial effect on some gram-negative/positive pathogenic microorganisms and lactic acid bacteria (by agar well diffusion, MIC test) and antioxidant capacity (DPPH, metal chelating, FRAP) of the oil extracted from Lavandula angustifolia (LA) by the clevenger method. As a result of the analysis, it was determined by headspace/GC-MS analysis that eucalyptol was the highest with the value of 22.58%, α-linalol was the second with the value of 19.44% and linalyl acetate was 14.61%. Lavandulol, essential component of Lavender, was determined by 1.39%. The highest antimicrobial effect of LA oil was found on Bacillus subtilus and MRSA (zone diameter of 24 to 22 mm, respectively), following on Staph. aureus. It was found that antibacterial effects on Staph. aureus and E. coli, Campliobacter jejuni, Salmonella poona, and lactic acid bacteria such as Lactobacillus reuteri and Lactobacillus plantarum (17, 16, 14, 14, 12, 12, 11 mm zone diameter). Therewithal, a significant antioxidative effect was determined on DPPH, FRAP and metal chelating analyzes. Also, the importance of its use in complementary medical and aroma therapy for preventive and symptomatic treatment of diseases. It is thought that present research will shed light on in vitro/in vivo studies to be carried out with LA oil.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67784567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Klorürün Şeker Pancarı Verim ve Kalite Değerleri Üzerine Etkisi","authors":"A. Pişkin","doi":"10.19159/tutad.1079341","DOIUrl":"https://doi.org/10.19159/tutad.1079341","url":null,"abstract":"Çalışma, klorür uygulamalarının şeker pancarı (Beta vulgaris L.) verim ve kalite değerlerine etkisini belirlemek amacıyla 2018 ve 2019 yıllarında Şeker Enstitüsü Eskişehir Deneme İstasyonunda yapılmıştır. Araştırmada şeker pancarının(Beta vulgaris L.) verim ve kalite değerlerinin ölçülmesi yanında uygulamaların bitki besin maddesi kapsamına etkisi değerlendirilmiştir. Denemeler her iki yılda da tesadüf blokları deneme desenine göre 4 tekrarlamalı olarak yürütülmüştür. Araştırma konuları klorürün 0.0, 2.0, 4.0, 6.0 ve 8.0 kg Cl da-1 uygulama düzeylerinden oluşmuştur. Birleştirilmiş varyans analiz sonuçlarına göre klorür; pancar kök verimi, şeker varlığı, sodyum kapsamı, potasyum kapsamı, zararlı azot kapsamı, arıtılmış şeker varlığı ve şeker verimi üzerine anlamlı bir etki yapmamıştır. Şeker pancarı verim ve kalite değerlerini artırmayan potasyum klorür kaynaklı klorür uygulamaları; bitkinin verim ve kalitesini düşürecek toksik bir etkide yapmamıştır. Klorürün uygulamaları ile verilen 0.0-16.8 kg da-1 arasındaki potasyum klorür gübre uygulanması orta sınıf klorür kapsayan deneme alanı topraklarında şeker pancarına toksik etki yapmadığı, verim ve kalitede değerlerinde de düşüşe neden olmadığı görülmüştür. Ayrıca klorür uygulamalarının bitkinin nitrat alımını engelleyerek azot kapsamını düşürmesiyle şeker pancarının şeker varlığında artış gerçekleşmesi stratejisinin çalışmadığı görülmüştür. Uygulamalar; şeker pancarı makro ve mikro bikri besin maddelerinden bitki N, P, K, Mg, S, Zn, Fe, Mn , Cu, ve B kapsamını değiştirmezken Ca ve Cl miktarını önemli ölçüde artırmıştır.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67783312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tüketicilerin Gıda Güvenliği Bilinç Düzeylerini Etkileyen Faktörler: Samsun İli Örneği","authors":"Uğur Başer, O. Kılıç, Gamze Aydın Eryılmaz","doi":"10.19159/tutad.1102254","DOIUrl":"https://doi.org/10.19159/tutad.1102254","url":null,"abstract":"Bu araştırma, Samsun İli Atakum İlçesindeki tüketicilerle yapılmıştır.","PeriodicalId":32452,"journal":{"name":"Turkiye Tarimsal Arastirmalar Dergisi","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48417297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}