Jurnal Akuakultur Indonesia最新文献

{"title":"The ontogenic study of early life stages of culture-bred Nomorhamphus sp. (Zenarchopteridae) from Lindu, Central Sulawesi","authors":"Muh. Herjayanto, O. Carman, Dinar Tri Soelistyowati, Alimuddin, Aryo Wenang Wicaksono, H. Arfah","doi":"10.19027/jai.22.2.179-186","DOIUrl":"https://doi.org/10.19027/jai.22.2.179-186","url":null,"abstract":"Nomorhamphus sp. is a freshwater fish that has been traded as an ornamental fish. This fish is unique as an endemic species with a halfbeak-like jaw and orange color on the caudal fin. However, this fish culture information needs a further information. Based on this condition, it is necessary to conduct a study as a basis for ornamental fish breeding and growing-out activities through domestication. A crucial problem in this fish is larval rearing, which can be observed through ontogeny studies. The study was conducted on the newly-born larval behavior, morphological development, andropodium development, growth, and survival rate at the early stages, namely larvae to juvenile. The results showed that the newly-born larvae of Nomorhamphus sp. Lindu had a total length of 1.6-1.8 cm. Larvae could swim four hours 22 minutes after birth and feed artemia nauplii with surface feeding type. The initial juvenile stage occurred 25 days of post-birth period with a total length of 2.0-2.2 cm. The water condition of the rearing during the study could support the larval transformation to juvenile. This study is the first report related to the aquaculture success of the early life stage of Nomorhamphus sp. Lindu at the domestication stage. \u0000  \u0000Keywords: andropodium, domestication, endemic halfbeak, larva development, surface feeding \u0000  \u0000ABSTRAK \u0000  \u0000Nomorhamphus sp. adalah ikan air tawar yang telah diperdagangkan sebagai ikan hias. Ikan ini memiliki keunikan pada statusnya sebagai spesies endemik, bentuk mulut menyerupai paruh setengah (halfbeak), dan warna oranye pada sirip ekor. Namun informasi budidayanya belum diketahui dengan baik. Karena itu perlu dilakukan kajian sebagai dasar dalam kegiatan pengembangbiakan dan pembesaran sebagai ikan hias melalui domestikasi. Salah satu kegiatan penting dalam budidaya yaitu pemeliharaan larva yang dapat diamati melalui studi ontogeni. Kajian pada studi awal ini dilakukan pada stadia awal hidup yaitu larva sampai juvenil. Pengamatan dilakukan pada tingkah laku larva pascalahir, perkembangan morfologi, perkembangan andropodium, pertumbuhan dan sintasan pada lingkungan budidaya. Hasil penelitian menunjukkan bahwa larva Nomorhamphus sp. Lindu yang baru dilahirkan memiliki panjang total 1,6-1,8 cm. Larva telah dapat berenang pada umur empat jam 22 menit pascalahir (pcl) dan bisa makan naupli artemia dengan tipe surface feeding. Stadia awal juvenil terlihat pada umur 25 hari pcl dengan ukuran panjang total 2,0-2,2 cm. Kondisi media pemeliharaan selama penelitian dapat mendukung kehidupan larva sampai juvenil. Penelitian ini merupakan catatan pertama terkait keberhasilan budidaya stadia awal hidup Nomorhamphus sp. Lindu pada tahap domestikasi. \u0000  \u0000Kata kunci: andropodium, domestikasi, ikan endemik, tipe makan permukaan, perkembangan larva","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81210500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kidney and liver histopathology of sea bass Lates calcarifer infected with black body syndrome-associated bacteria","authors":"S. Nuryati, Mauliza Yanti, Rahman, S. Wahyuwardani","doi":"10.19027/jai.22.2.170-178","DOIUrl":"https://doi.org/10.19027/jai.22.2.170-178","url":null,"abstract":"Bacterial isolation was carried out on sea bass Lates calcarifer juvenile with black body syndrome (BBS) and isolates of Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, and Pseudomonas stutzeri L02 were obtained. Microscopic anatomical pathology of each bacterium needs to be done to determine its pathogenicity. This study aims to compare the degree of damage to sea bass juveniles' liver and kidney organs through histopathological observations due to bacterial infections that cause BBS. The degree of histopathological damage was determined by scoring histopathological changes in the tissue under a microscope with a magnification of 40×10 in five fields of view. The data obtained were analyzed using SPSS 24 software and further tested with Kruskal-Wallis and Post Hoc (Mann Whitney). The results showed that fish liver and kidney organs in all treatments experienced necrosis compared to the control treatment, but not significantly different between bacterial infection treatments. Histopathological observations of kidney and liver organs of sea bass juveniles infected with bacteria that cause BBS show necrosis damage that was classified as normal and mild. \u0000  \u0000Keywords: bacteria, histopathology, Lates calcarifer \u0000  \u0000ABSTRAK \u0000  \u0000Isolasi bakteri telah dilakukan pada benih ikan kakap putih yang mengalami black body syndrome (BBS) dan diperoleh isolat bakteri Pseudomonas stutzeri L01, Vibrio harveyi, Bacillus cereus, Salinicoccus roseus, dan Pseudomonas stutzeri L02. Patologi anatomi mikroskopis dari masing-masing bakteri perlu dilakukan untuk mengevaluasi patogenitasnya. Penelitian ini bertujuan membandingkan tingkat kerusakan organ hati dan ginjal benih ikan kakap putih melalui pengamatan histopatologi akibat infeksi bakteri penyebab BBS. Derajat kerusakan histopatologi ditentukan dengan melakukan skoring perubahan histopatologi jaringan di bawah mikroskop dengan perbesaran 40×10 pada lima lapang pandang. Data dianalisis menggunakan software SPSS 24 dan diuji lanjut dengan Kruskal-Wallis dan Post Hoc (Mann Whitney). Hasil pengamatan menunjukkan bahwa organ hati dan ginjal ikan pada semua perlakuan infeksi mengalami nekrosis dibandingkan dengan perlakuan kontrol, tetapi tidak berbeda nyata antar perlakuan infeksi bakteri. Hasil pengamatan histopatologi organ ginjal dan hati benih ikan kakap putih yang diinfeksi dengan bakteri yang menyebabkan BBS menunjukkan kerusakan nekrosis yang tergolong pada derajat normal dan ringan. \u0000  \u0000Kata kunci: bakteri, histopatologi, kakap putih","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73477234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production and business performance of Anguilla bicolor fingerlings in a recirculation system with different stocking densities","authors":"T. Budiardi, I. Diatin, Kriswidya Arlita, A. Vinasyiam, A. Sudrajat, M. Setiawati, R. Affandi, M. Kamal, R. I. Wahju, M. Nurilmala","doi":"10.19027/jai.22.2.156-169","DOIUrl":"https://doi.org/10.19027/jai.22.2.156-169","url":null,"abstract":"Eel (Anguilla bicolor) in the grow-out culture requires good fingerling seeds. Increasing the eel productivity can be done by increasing the stocking density, that should be balanced with good environmental and feed management. This study aimed to analyze the production and business performance of fingerlings in a recirculation system to increase the eel survival and growth rate. The study used a completely randomized design with three different stocking densities, namely 4 g/L (A), 5 g/L (B), and 6 g/L (C). The average weight of each fingerling was 20 ± 4.09 g, that was kept in a 1.5-m3 pond with a recirculation system. Feeding was performed two times a day using commercial feed with probiotic supplementation. The results showed that different stocking densities significantly affected feed conversion ratio, total biomass weight, and coefficient of variance. However, different stocking densities had no significant effect on survival rate, absolute weight growth rate, specific weight growth rate, and condition factor. The C treatment obtained the highest profit with an R/C ratio of 1.20 ± 0.03. The best production and cultivation performance of eel fingerling in a recirculation system with different stocking densities is found in treatment C (6 g/L). \u0000  \u0000Keywords: Anguilla bicolor, business performance, production performance, recirculation system, stocking density \u0000  \u0000ABSTRAK \u0000  \u0000Budidaya ikan sidat (Anguilla bicolor) pada segmen pembesaran memerlukan benih yang baik khususnya untuk benih fingerling. Upaya peningkatan produksi benih ikan sidat dapat dilakukan dengan peningkatan padat tebar yang diiringi dengan manajemen lingkungan dan pakan yang baik. Tujuan penelitian ini menganalisis kinerja produksi dan kinerja usaha pada pemeliharaan fingerling dalam sistem resirkulasi sehingga meningkatkan kelangsungan hidup dan pertumbuhan. Penelitian menggunakan rancangan acak lengkap yang terdiri atas 3 perlakuan padat tebar dengan tiga ulangan, yaitu padat tebar 4 g/L (A), 5 g/L (B), dan 6 g/L (C). Fingerling  ikan sidat yang digunakan berbobot awal 20 ± 4,09 g/ekor, yang dipelihara dalam bak 1,5 m3 dengan sistem resirkulasi. Pakan diberikan dua kali sehari berupa pakan buatan komersial yang diberi probiotik. Hasil penelitian menunjukkan bahwa padat tebar berpengaruh nyata terhadap parameter rasio konversi pakan, laju pertumbuhan mutlak biomassa, dan koefisien keragaman bobot tetapi tidak berpengaruh nyata terhadap tingkat kelangsungan hidup, laju pertumbuhan mutlak bobot, laju pertumbuhan spesifik bobot, dan faktor kondisi. Hasil analisis kinerja usaha budidaya fingerling dengan padat tebar berbeda menunjukkan berbeda nyata dan sebanding dengan kinerja produksi. Perlakuan C memberikan keuntungan tertinggi dengan rasio R/C sebesar 1,20 ± 0,03. Kinerja produksi dan kinerja usaha budidaya fingerling ikan sidat (Anguilla bicolor) dalam sistem resirkulasi dengan padat tebar berbeda terbaik terdapat pada perlakuan C (6 g/L). \u0000  \u0000Kata kunci: Anguilla bicolor, ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80022383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of Trophic Status and Floating Net Cages Carrying Capacity in Jatigede Reservoir","authors":"Kristina Marsela, Dadan Sumiarsa, Denny Kurniadie","doi":"10.19027/jai.22.2.147-155","DOIUrl":"https://doi.org/10.19027/jai.22.2.147-155","url":null,"abstract":"Aquaculture activities with floating net cages are essential to increase fishery production. The existence of the fish cages in the Jatigede Reservoir is illegal because it is against local government regulations. However, the people affected by the dam's construction still maintain it for their economic reasons. Therefore, it is necessary to do a research to overcome these problems by determining the carrying capacity of aquaculture and evaluating the trophic status of the waters. However, the people affected by the dam construction still maintain it for their economic reasons. Determination of the carrying capacity of fish farming in the Jatigede Reservoir was conducted using the Beveridge method by calculating the remaining phosphorus still available in the Jatigede Reservoir and evaluating the trophic state index based on three indicators: transparency, total phosphorus, and chlorophyll-a. The sampling was carried out by purposive sampling method. The calculation results show that the condition of the Jatigede Reservoir is eutrophic to hiperthropic with a trophic state index (TSI) value of 66-71. The carrying capacity of the reservoir is 7,140.25 tons of fish/year. The development of cage aquaculture that exceeds the aquaculture carrying capacity  in Jatigede Reservoir. Reducing cage aquaculture fish production following its carrying capacity is important to preserve the environment and control eutrophication.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"174 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76114558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary supplementation of Bacillus sp. NP5 and dayak onion simplicia powder Eleutherine bulbosa (Mill.) Urb. for the prevention of Aeromonas hydrophila in catfish Clarias sp.","authors":"R. Sudrajat, M. Yuhana, W. Widanarni, J. Ekasari, U. Afiff","doi":"10.19027/jai.22.2.134-146","DOIUrl":"https://doi.org/10.19027/jai.22.2.134-146","url":null,"abstract":"Aeromonas hydrophila is the main causative agent of ulcerative disease in catfish and causes considerable economic losses to Indonesian aquaculture. This study evaluates the prebiotic activity and the effect of feed supplementation of dayak onion simplicia powder (DOSP) on the immune response and survival of catfish infected with A. hydrophila. Five doses (0.20, 0.25, 0.30, 0.35, and 0.40 g/mL) of DOSP were tested in vitro to assess the prebiotic activity score. The results showed that a dose of 0.20 g/mL gave a significantly (P<0.05) higher probiotic stimulation value than other doses. In the in vivo test, the study used a completely randomized design with five treatments, namely simplicia (DOSP 20 g/kg), probiotic (PRO, Bacillus sp. NP5 108 CFU/mL, 1% v/w), combination (PRO+DOSP), and control (positive and negative). Fish were reared for 45 days and fed three times a day. On day 46, fish from all treatments, except negative control, were infected with an A. hydrophila dose of 106 CFU/mL injected intramuscularly. The results showed that the combination treatment (PRO+DOSP) gave better total erythrocytes, hemoglobin, hematocrit, total leukocytes and phagocytosis activity than probiotics, DOSP, and control. Administering the combination (PRO+DOSP) can reduce the total number of A. hydrophila lower than the probiotic, DOSP, and control treatments. In addition, the survival rate of catfish in the combined treatment (PRO+DOSP) was significantly (P<0.05) higher than probiotics, DOSP, and control. The results of this study can be a helpful reference and application for the early prevention of A. hydrophila infection. \u0000  \u0000Keywords: aquaculture, Bacillus sp. NP5, dayak onion, probiotics, simplicia powder \u0000  \u0000ABSTRAK \u0000  \u0000Aeromonas hydrophila adalah penyebab utama penyakit bercak merah pada budidaya ikan lele dan menyebabkan kerugian ekonomi cukup besar pada akuakultur Indonesia. Penelitian ini bertujuan untuk mengevaluasi aktivitas prebiotik serbuk simplisia bawang dayak (SSBD) dan pengaruh penambahan kombinasi probiotik Bacillus sp. NP5 dan SSBD pada pakan terhadap respons imun dan kelangsungan hidup ikan lele yang diinfeksi A. hydrophila. Lima dosis (0,20, 0,25, 0,30, 0,35, dan 0,40 g/mL) SSBD diuji secara in vitro untuk menilai skor aktivitas prebiotik. Hasil penelitian menunjukkan bahwa dosis 0,20 g/mL memberikan nilai stimulasi probiotik signifikan (P<0,05) lebih tinggi dibandingkan dosis lainnya. Pada uji in vivo, penelitian menggunakan rancangan acak lengkap terdiri dari lima perlakuan, yaitu simplisia (SSBD, serbuk simplisia bawang dayak 20 g/kg), probiotik (PRO, Bacillus sp. NP5 108 CFU/mL, 1% (v/w), kombinasi (PRO+SSBD), dan kontrol (positif dan negatif). Ikan dipelihara selama 45 hari dan diberi pakan tiga kali sehari. Pada hari ke 46, ikan pada semua perlakuan, kecuali kontrol negatif, di infeksi A. hydrophila dosis 106 CFU/mL secara intramuskular. Hasil penelitian menunjukkan bahwa kombinasi (PRO+SSBD) memberikan total eritrosit, hemoglobin, ","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88689218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pollution index and load of total nitrogen and phosphate on agriculture and fisheries in Jatigede Reservoir","authors":"Zulfia Kamila Mutia, D. Kurniadie, D. Sumiarsa","doi":"10.19027/jai.22.2.126-133","DOIUrl":"https://doi.org/10.19027/jai.22.2.126-133","url":null,"abstract":"Jatigede Reservoir has an area of approximately 4,122 Ha, the purpose of Jatigede dam construction is to increase rice production with a range irrigation network system. Problems arising from the use of chemical fertilizers, regarding the development of agricultural land used to increase agricultural productivity, support food security and also activities in floating net cages (FNC) can result in a decrease in reservoir water quality, siltation of reservoirs, etc. Organic waste from FNC cultivation feed, for example nitrogen and phosphate. The purpose is to determine the pollution index of each observation station and the concentration of total nitrogen and total phosphor pollutant loads from the agriculture and fisheries sectors in Jatigede Reservoir so that it can be analyzed which activities have the most influence on pollution and determine effective countermeasures a decrease in water quality in Jatigede Reservoir. The quantitative method uses a survey method is processing data from laboratory tests using the Pollution Index and total nitrogen and phosphate pollution loads. The results of the highest pollution index are at station three of 6.08 with moderate pollution status caused by runoff from Cihonje inlet waste and FNC activities. The nitrogen parameter pollution load has a high amount of 192.13 kg/day then the amount of phosphate pollution load is 34.16 kg/day. As for good pollution control, by reducing the burden of pollution by involving the community in managing the reservoir environment, routine monitoring of reservoir water quality and mapping potential pollutant sources at each location so that problems will be quickly resolved. \u0000  \u0000Keywords: agriculture, fisheries, Jatigede reservoir, pollution index, pollution load \u0000  \u0000ABSTRAK \u0000  \u0000Waduk Jatigede memiliki luas sekitar 4.122 Ha, tujuan pembangunan Waduk Jatigede adalah untuk meningkatkan produksi padi dengan sistem jaringan irigasi rentang. Permasalahan yang ditimbulkan terkait penggunaan pupuk kimiawi, mengenai perkembangan lahan pertanian yang digunakan untuk meningkatkan produktivitas pertanian dan mendukung ketahanan pangan dan juga kegiatan budidaya ikan karamba jaring apung (KJA) dapat mengakibatkan terjadinya penurunan kualitas air waduk, pendangkalan waduk, dan lain-lain. Limbah organik sisa pakan budidaya KJA yang terbuang ke dalam perairan contohnya yaitu nitrogen dan fosfat. Tujuan penelitian ini adalah untuk menentukan index pencemaran dari setiap stasiun pengamatan dan konsentrasi beban pencemar total nitrogen dan total fosfat dari sektor pertanian dan perikanan di Waduk Jatigede sehingga dapat dianalisis kegiatan mana yang paling berpengaruh terhadap pencemaran dan menentukan penanggulangan yang efektif untuk mencegah penurunan kualitas air di Waduk Jatigede. Metode yang digunakan adalah mix method (metode campuran). Metode kuantitatif menggunakan metode survey yaitu pengolahan data hasil uji laboratorium. Menganalisis tingkat pencemaran di setiap stasiun, menggunak","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78560260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a real-time duplex PCR assay for simultaneously diagnosis of KHV and CEV in common carp and koi","authors":"Khumaira Puspasari, Adityawati Fajar Rini, Noviana Dewi, Ratih Ismayasari","doi":"10.19027/jai.22.2.115-125","DOIUrl":"https://doi.org/10.19027/jai.22.2.115-125","url":null,"abstract":"Koi herpesvirus (KHV) and carp edema virus (CEV) are potential risks for the koi trade and for global common carp aquaculture. Due to the severe impact of both viruses, a robust diagnostic tool was required, capable of detecting and distinguishing both infections with high accuracy and precision. The aim of this study was to describe a real-time duplex TaqMan PCR assay for simultaneous detection of KHV and CEV in common carp and koi. Two pairs of primers with two TaqMan probes were used to amplify specific and conserved regions of KHV and CEV DNA. This assay was confirmed to be sensitive and specific. Limit of detections of the assay were 15 DNA copies/µL for KHV and 150 DNA copies/µL for CEV, respectively. This assay was able to identify and distinguish CEV and KHV, but was unable to identify other pathogens and sample matrices. For clinical validation, 18 KHV and CEV positive samples each, as well as 12 negative samples were tested with three different test methods, i.e., real-time duplex PCR, real-time simplex PCR, and conventional PCR. All three tests provide optimal conformity of results. The results showed that real-time duplex PCR was able to detect the presence and distinguish each pathogen in infected fish. This real-time duplex PCR assay is a rapid, sensitive, and specific test for detecting KHV and CEV in carp fish, thus it can be considered a valid alternative assay in aquaculture clinical laboratories. \u0000  \u0000Keywords: Cyprinus carpio, CEV, diagnostic, KHV, real-time duplex PCR \u0000  \u0000ABSTRAK \u0000  \u0000Koi herpesvirus (KHV) dan carp edema virus (CEV) merupakan risiko potensial untuk perdagangan koi dan budidaya ikan mas global. Karena dampak dari keduanya yang parah, maka diperlukan perangkat diagnostik yang tangguh, yang mampu mendeteksi dan membedakan keduanya dengan akurasi dan presisi yang tinggi. Tujuan dari penelitian ini adalah menjabarkan pengujian real time TaqMan PCR dupleks untuk mendeteksi KHV dan CEV pada ikan mas dan koi secara simultan dalam satu reaksi. Dua pasang primer dengan dua TaqMan probe digunakan untuk mengamplifikasi wilayah spesifik dan lestari dari DNA KHV dan CEV. Pengujian ini terbukti sensitif dan spesifik. Sensitivitas dari pengujian ini adalah 15 kopi DNA/µL untuk KHV dan 150 kopi DNA/µL untuk CEV. Pengujian ini mampu mengidentifikasi dan membedakan CEV dan KHV, tetapi tidak dapat mengidentifikasi patogen lain dan matriks sampel. Untuk validasi klinis, masing-masing 18 sampel positif KHV dan CEV, serta 12 sampel negatif diuji dengan tiga metode pengujian yang berbeda, yaitu real-time PCR dupleks, real-time PCR tunggal dan PCR konvensional. Ketiga pengujian tersebut memberikan kesesuaian hasil yang optimal. Hasil penelitian menunjukkan bahwa real-time PCR dupleks mampu mendeteksi keberadaan dan membedakan setiap patogen pada ikan yang terinfeksi. Uji real-time PCR dupleks ini merupakan pengujian yang cepat, sensitif, dan spesifik untuk mendeteksi KHV dan CEV pada ikan koi dan mas, sehingga dapat dipertimbangkan seb","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81574160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Health Status of Hybrid Grouper Epinephelus fuscoguttatus (♀) x E. lanceolatus (♂) Cultured in Floating-net Cage at Kelapa Dua Island, Seribu Islands, Indonesia","authors":"Ruku R. Borut, K. Nirmala, I. Effendi, Widanarni","doi":"10.19027/jai.22.2.106-114","DOIUrl":"https://doi.org/10.19027/jai.22.2.106-114","url":null,"abstract":"This study aimed to identify the fish health status, based on the total erythrocytes (cells/mm3), total leukocytes (cells/mm3), hemoglobin level (g %), hematocrit, and histopathological profiles. This study was performed on February, 2021 – January, 2022. The study was located in floating-net cage of Kelapa Dua Island, Seribu Islands, Jakarta, Indonesia. Sampling was performed by a purposive-sampling method, environmental parameter measurements, containing temperature and salinity, clinical symptom observation in fish, total erythrocytes, leukocytes, hemoglobin, hematocrit, histopathological profiles of hybrid grouper liver and kidney, and existence of VNN virus. The observation results present the behavior changes in sick fish, namely whirling on the net cage surface, dead-sleeping or death-like condition below the net cage. Hybrid grouper has total erythrocytes of 1.36 x 106 – 5.13 x 106 cells/mm3, total leukocytes of 2.9 x 104 – 7.9 x 104 cells/mm3, hemoglobin level of 5.56 - 10.94 g %, and hematocrit of 13.31 - 24.78 %. From the histopathological observation, hepatocyte, necrosis, vacuolysis was occurred in liver and tubular, leukocyte infiltasi, nicrosis was occurred in kidney and with the highest prevalence of VNN at 20 – 80 %. Therefore, hybrid grouper is sick due to increased number of leukocytes at 5.13 x 106 cells/mm3 above the normal range, followed by decreased hemoglobin and hematocrit levels at 5.56 - 10.94 g % and 13.31 - 24.78 %, respectively, due to organ damage.     \u0000  \u0000Keyword: must be written in not more than 5 words and alphabeticaly listed \u0000  \u0000ABSTRAK \u0000  \u0000Penelitian ini bertujuan untuk mengetahui status kesehatan ikan meliputi total eritrosit (sel/mm3), total leukosit (sel/mm3), kadar hemoglobin (gram %) dan hematokrit (%) dan gambaran histopatolgi. Penelitian ini dilaksanakan pada periode bulan Februari 2021 hingga Januari 2022. Lokasi penelitian pada keramba jaring apung (KJA) di Pulau Kelapa Dua Kepulauan Seribu. Metode pengambilan sampel ikan dilakukan secara purposive sampling, pengukuran parameter lingkungan perairan yaitu suhu dan salinitas, pengamatan gajala klinis ikan, perhitungan jumlah eritrosit, leukosit, kadar hemoglobin dan hematokrit serta gambaran histopatologi pada organ hati dan ginjal ikan kerapu cantang dan keberadaan virus VNN. Hasil pengamatan menunjukkan perubahan tingkah laku ikan sakit yaitu  berputar ke atas permukaan KJA, terjadi sleeping dead atau ikan berada di dasar seperti mati. Ikan kerapu cantang memiliki jumlah eritrosit 1.36 x 106 – 5.13 x 106 sel/mm3, leukosit 2.9 x 104 – 7.9 x 104 sel/mm3, hemoglobin 5.56 - 10.94 gr  %, dan hematokrit 13.31 - 24.78 %, hasil histopalogi ikan mengalami perubahan sel pada hati yaitu hepatosit, vakuolisasi, nekrosis dan ginjal mengalami perubahan infiltrasi leukosit, nicrosis, tubulus dan kehadiran virus VNN tertinggi berkisar 20 % - 80 %. Berdasarkan hasil penelitian tersebut maka dapat disimpulkan bahwa ikan kerapu cantang yang sakit karena jumlah leukosit","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"81 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84121060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pathogenicity test bacteria from Oreochromis niloticus and Clarias gariepinus aquaculture ponds","authors":"Esti Handayani Hardi, Aldi M. Halim, R. Nugroho, Mira Mawardi, A. Isnansetyo, Angela Mariana Lusiastuti, Widianingsih Rahayu, Andi Niklani, G. Saptiani","doi":"10.19027/jai.22.1.97-105","DOIUrl":"https://doi.org/10.19027/jai.22.1.97-105","url":null,"abstract":"Present research aimed to examine the pathogenicity opportunities of some bacteria which were isolated from freshwater aquaculture ponds to some fish Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius. The bacteria were isolated from the water and feces of O. niloticus and C. gariepinus aquaculture in Samarinda, which were identified as Escherichia coli, Enterobacter cloacae, and Enterobacter amnigenus. An 0.1 mL-1 of each bacteria was intramuscular injection and evaluated the clinical signs, eksternal pathology, and fish mortality. The freshwater fish using in this research were Oreochromis niloticus, Clarias gariepinus, and Pangasius pangasius with size 10-15 g from Loa Kulu, Kutai Kartanegara Regency, East Kalimantan, Indonesia. The result showed that each bacteria caused mortality in fish: E. coli bacteria caused fish mortality 23.33-66.67%; E. cloacae 10.00 to 90.00%; and E. amnigenus by 3.33-56.67%. The average death time of E. coli and E. amnigenus in all three fish were under 48 hours, while E. cloacae caused mortality less than 48 hours in C. gariepinus and P. pangasius. In conclusion, E. coli, E. cloacae, and E. amnigenus are putative pathogenic bacterium in O. niloticus, C. gariepinus, and P. pangasius.","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90786676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mini-review utilization of Vibrio parahaemolyticus virulence coding genes for early detection of acute hepatopancreatic necrosis disease (AHPND)","authors":"M. Yuhana, U. Afiff","doi":"10.19027/jai.22.1.87-96","DOIUrl":"https://doi.org/10.19027/jai.22.1.87-96","url":null,"abstract":"The ability to track the presence of pathogenic Vibrio parahaemolyticus, an AHPND causative agent in shrimp is one of the keys to controlling this infectious disease. A reputable disease diagnosis is appreciated as the ability to track the pathogenic infection when the host abnormality is undetectable due to the low pathogenic cell concentration. This mini-review article discusses the selected virulence encoding genes as molecular markers and the steps of standard validation methods in the application for early detection of AHPND disease. The proper diagnosis method is crucial to prevent the spread of Vibriosis AHPND which significantly results in economic losses for shrimp farmers. In this early warning system, we need a molecular method available for quick detection by applying the tracking tools that can discriminate pathogenic V. parahaemolyticus AHPND strain. Several types of potential genes that can be developed into tracking devices for infectious Vibriosis are pathogenic genes encoding the virulence factor. Through several stages of testing the selected virulence encoding genes will be developed into molecular markers. The polymerase chain reaction method and several of its variants have been widely applied using selected molecular markers. Furthermore, the use of molecular markers for the diagnosis of AHPND disease in shrimp must be validated by determining aspects of sensitivity, detection specificity, repeatability consistency, and reproducibility \u0000  \u0000Keywords: virulence-encoding gene, Vibrio parahaemolyticus, AHPND, diagnose, molecular marker \u0000  \u0000ABSTRAK \u0000  \u0000Kemampuan melacak keberadaan patogen Vibrio parahaemolyticus, agen penyebab AHPND pada udang adalah salah satu kunci untuk mengendalikan penyakit menular ini. Diagnosis penyakit yang memiliki reputasi baik akan dihargai karena mampu untuk melacak infeksi patogen ketika tanda-tanda abnormalitas pada inang belum terdeteksi karena konsentrasi sel patogen yang masih rendah. Makalah mini-review ini membahas tentang tahap-tahap metode validasi standardalam aplikasi gen penyandi virulensi terseleksi untuk deteksi dini penyakit AHPND. Metode diagnosis yang tepat sangat penting untuk mencegah penyebaran Vibriosis AHPND yang secara signifikan mengakibatkan kerugian ekonomi bagi petambak udang. Dalam sistem peringatan dini ini, diperlukan metode molekular yang tersedia untuk deteksi cepat dengan menerapkan alat pelacak yang mampu membedakan patogen V. parahaemolyticus strain AHPND. Beberapa jenis gen potensial yang dapat dikembangkan menjadi alat pelacak penyakit Vibriosis menular adalah gen patogen yang mengkodekan faktor virulensi. Beberapa tahapan pengujian harus dilakukan untuk menjadikan gen penyandi virulensi terpilih sebagai kandidat yang akan dikembangkan menjadi penanda molekular. Metode polymerase chain reaction dan beberapa variannya telah banyak diterapkan dengan menggunakan penanda molekular terseleksi. Selanjutnya pemanfaatan penanda molekular untuk diagnosis penyakit AHPND pad","PeriodicalId":32090,"journal":{"name":"Jurnal Akuakultur Indonesia","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85526856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}