Scientific Dental Journal最新文献_第4页

Immunocytochemistry and western blot test for the in-situ detection of biomarkers of osteogenesis 免疫细胞化学和蛋白质印迹法原位检测成骨生物标志物

Scientific Dental Journal Pub Date : 2022-01-01 DOI: 10.4103/SDJ.SDJ_101_21

Priska Natassya, E. Bachtiar

{"title":"Immunocytochemistry and western blot test for the in-situ detection of biomarkers of osteogenesis","authors":"Priska Natassya, E. Bachtiar","doi":"10.4103/SDJ.SDJ_101_21","DOIUrl":"https://doi.org/10.4103/SDJ.SDJ_101_21","url":null,"abstract":"There are two ways that bones can form: intramembranous and endochondral ossification. Specific osteogenic markers, such as insulin-like growth factor (IGF-1), osteocalcin (OCN), Runt-related transcription factor 2 (RUNX2), and osterix (OSX), accompany osteoblast differentiation from an undifferentiated state to a functional state. IGF-1 hormones are the main regulators in growth, differentiation, and apoptosis in cells and tissues mediated by IGF-1 receptors (IGF-1R). Biomolecular technology aims to study nucleic acids and their regulation and expression of proteins. Techniques that can be used when analyzing proteins include the Bradford protein assay, immunocytochemistry, immunohistochemistry, sodium dodecyl sulfate–polyacrylamide gel electrophoresis, and the western blot test. To better understand the biomarkers of osteogenesis, the use of in-situ detection is suggested, such as immunocytochemistry and the western blot test. For this review, the author adhered to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) standards. The research examined 50 articles; only 4 articles were selected for this review. In the immunocytochemical test, IGF-1 expression was found in the nucleus and IGF-1R expression in the cell membrane, and it was found that RUNX2, OSN, and OCN are important for osteogenesis. During the western blot test, it was found that the addition of IGF-1 in dental stem cells could increase the expression of RUNX2, OSX, and OCN proteins. Based on this information, it appears that both immunocytochemistry and western blot tests can detect the main biomarkers of osteogenesis.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"6 1","pages":"10 - 17"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47217594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

White lesions that can be wiped off: A case report of oral mucosal peeling 可擦去的白色病变:口腔黏膜脱皮1例

Scientific Dental Journal Pub Date : 2022-01-01 DOI: 10.4103/SDJ.SDJ_96_21

A. Astuti, Febrina Rahmayanti

{"title":"White lesions that can be wiped off: A case report of oral mucosal peeling","authors":"A. Astuti, Febrina Rahmayanti","doi":"10.4103/SDJ.SDJ_96_21","DOIUrl":"https://doi.org/10.4103/SDJ.SDJ_96_21","url":null,"abstract":"Background: Oral mucosal peeling (OMP) refers to an asymptomatic white lesion commonly encountered in daily practice but rarely discussed in the literature. OMP can be induced by various daily oral care products. Although common, some clinicians may not be familiar with OMP. In this case report, we describe a case of widespread OMP associated with toothpaste and mouthwash use. Case Report: A 40-year-old man presented with asymptomatic white lesions, with thin sloughing appearance in the whole oral mucosa. The patient was healthy, with no routine use of medications. The lesions were easily removed using a sterile gauze, with no subsequent erosion or ulceration occurring. The lesions were attributed to the use of sodium lauryl sulfate (SLS)-containing toothpaste and a mouthwash containing chlorhexidine (CHX) and essential oils. The patient was advised about the benign nature of the lesions. Discontinuation of SLS-containing toothpaste was suggested. Conclusion: OMP is commonly associated with the use of SLS-containing toothpastes and dentifrices and mouthwashes containing essential oils or CHX with the use of SLS-containing toothpastes and dentifrices and mouthwashes containing essential oils or CHX. Dentists must be able to identify OMP and provide appropriate patient education.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"6 1","pages":"53 - 57"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47174868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A cross-sectional study on the role of film stars and peers in smoking initiation and tobacco use among male adolescents aged 13–15 years in Chennai city, Tamil Nadu, India 印度泰米尔纳德邦金奈市电影明星和同龄人在13-15岁男性青少年开始吸烟和使用烟草中的作用的横断面研究

Scientific Dental Journal Pub Date : 2022-01-01 DOI: 10.4103/SDJ.SDJ_110_21

Anusha Raghavan, Nishanthi Murali, Dian Alba, A. Sukumaran, Madan Diwakar

{"title":"A cross-sectional study on the role of film stars and peers in smoking initiation and tobacco use among male adolescents aged 13–15 years in Chennai city, Tamil Nadu, India","authors":"Anusha Raghavan, Nishanthi Murali, Dian Alba, A. Sukumaran, Madan Diwakar","doi":"10.4103/SDJ.SDJ_110_21","DOIUrl":"https://doi.org/10.4103/SDJ.SDJ_110_21","url":null,"abstract":"Background: Exposure to tobacco at a young age leads to a subsequent smoking habit in large proportions of the population in adulthood. Social and mass media have both positive and negative effects on adolescents’ behavior and social skills and therefore play vital roles in driving this behavior. Objectives: The purpose of this study was to assess the influence of film stars on smoking initiation and tobacco use among male adolescents aged 13–15 years in Chennai city, Tamil Nadu, India. Methods: The study adopted a cross-sectional design. In total, 200 students from schools that serve low-income households participated in the study. All the students completed a 20-item questionnaire on the perceived severity of smoking, perceived barriers to quitting, and factors affecting quitting. Results: None of the participants had a history of smoking. One (0.5%) participant stated that he smoked daily. All the participants agreed that exposure to media images of smoking behavior among their favorite film stars would not encourage them to smoke. In terms of peer pressure, one (0.5%) participant stated that he would accept a cigarette if offered one by a friend. Only descriptive information of the collected data was tabulated as the aim of the study was to collect the baseline data and not to test any prespecified hypothesis. Conclusion: In our study, peer pressure seemed to play more influence on the smoking initiation by adolescents than the film stars on mass media. Though all of them agreed that they would not be provoked by media images of film stars smoking, one participant was likely to initiate the habit on being offered by his friend. Hence, the evidence suggests the need for reorientation of research modalities to better identify early initiators of smoking.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"6 1","pages":"48 - 52"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70843355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Dissolving efficacy of xylene on epoxy resin-based and bioceramic-based root canal sealers 二甲苯在环氧树脂基和生物陶瓷基根管密封剂上的溶解效果

Scientific Dental Journal Pub Date : 2022-01-01 DOI: 10.4103/SDJ.SDJ_86_21

C. Willie, Aryadi

{"title":"Dissolving efficacy of xylene on epoxy resin-based and bioceramic-based root canal sealers","authors":"C. Willie, Aryadi","doi":"10.4103/SDJ.SDJ_86_21","DOIUrl":"https://doi.org/10.4103/SDJ.SDJ_86_21","url":null,"abstract":"Background: Epoxy resin-based and bioceramic-based root canal sealers are used in endodontic treatment as root canal obturation materials because they are considered to have many advantages. The complete removal of these materials during root canal retreatment is crucial in order to ensure adequate disinfection. Due to the difficulty in accessing root canal ramifications, removal of these filling materials without damaging the teeth can be successfully achieved using endodontic solvents. Objective: The aim of this study was to evaluate the solubility of two types of root canal sealers in xylene used in endodontics. Methods: A total of 34 epoxy resin and bioceramic samples were stored for 72 h in an incubator to set. The samples were then immersed for 2 min in 1 mL of xylene. The excess xylene on the samples was drained with an absorbent paper and stored in an incubator for 24 h to dry. The pre- and post-immersion weight of the samples was measured using a digital analytical scale, then the weight difference between the groups was analyzed using the independent t test at a 5% significance level. Results: No significant difference was observed between the epoxy resin-based root canal sealer group and the bioceramic-based root canal sealer group (P ≥ 0.05). Conclusion: Xylene endodontic solvent has a similar efficacy for removing epoxy resin-based and bioceramic-based root canal sealers in in vitro study.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"6 1","pages":"32 - 35"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46934046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial efficacy of copper nanoparticles against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: An in-vitro study 铜纳米颗粒抗放线菌和牙龈卟啉单胞菌的体外研究

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_84_21

S. Mahale, S. Shimpi, K. Sethi, D. Chaudhari, P. Kadam, Ankita Katkurwar

{"title":"Antimicrobial efficacy of copper nanoparticles against Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis: An in-vitro study","authors":"S. Mahale, S. Shimpi, K. Sethi, D. Chaudhari, P. Kadam, Ankita Katkurwar","doi":"10.4103/sdj.sdj_84_21","DOIUrl":"https://doi.org/10.4103/sdj.sdj_84_21","url":null,"abstract":"Background: Plaque accumulation on teeth surfaces and prosthetic or orthodontic appliances present a serious challenge for the maintenance of oral health. Copper nanoparticles (NPs) can be incorporated into coatings and applied to restorative materials to prevent plaque formation and the progression of periodontal diseases. Objective: The aim of this article is to evaluate the antimicrobial efficacy of copper NPs against selected periodontal pathogens (Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans). Methods: The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of copper NPs were determined using broth dilution assay. Copper NPs (size: 30—50 nm, purity: 99.9%) were used for the study. Results: Both P. gingivalis and A. actinomycetemcomitans were sensitive to copper NPs. Nano-copper had a bactericidal effect against P. gingivalis at a concentration of 0.8 μg/mL and a bacteriostatic effect against the bacterium at a concentration of 0.4 μg/mL. For A. actinomycetemcomitans, nano-copper had a bactericidal effect at a concentration of 3.12 μg/mL and a bacteriostatic effect at a 1.6 μg/mL concentration. Conclusion: Nano-copper exhibits an antibacterial effect against periodontal pathogens. Future studies are needed to explore the applicability of these copper-based antimicrobial agents in clinical settings.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"128 - 132"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43831537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Relationship between nutritional status and mandibular length in subjects aged 10–16 years 10-16岁受试者营养状况与下颌长度的关系

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_32_20

H. Lubis, Rachel Tiong

{"title":"Relationship between nutritional status and mandibular length in subjects aged 10–16 years","authors":"H. Lubis, Rachel Tiong","doi":"10.4103/sdj.sdj_32_20","DOIUrl":"https://doi.org/10.4103/sdj.sdj_32_20","url":null,"abstract":"Background: Malocclusion is a significant dental health problem in Indonesia. Malocclusion has multifactorial causes, including nutrition. Malnutrition may have an adverse effect on mandibular length and lead to tooth malposition. Objective: The aim of this article is to investigate the relationship between nutritional status and mandibular length in subjects aged 10–16 years. Methods: This was an observational analytic study with a cross-sectional design. The sampling method was purposive sampling. The subjects were 100 children (50 children with normal nutrition and 50 children with nutrition lower than normal) aged 10–16 years from Al-Ikhlas Islamic Elementary School and Advent 2 School in Medan, with no history of orthodontic treatment and facial trauma and not wearing prostheses. All the subjects were photographed with their heads held in a natural position. The photographs were analyzed using a computer program CorelDraw X7 to measure the length of the mandible. All statistical analyses were performed using SPSS. Univariate analyses were performed to obtain the mean and standard deviation (SD) values of all parameters. Descriptive statistics were obtained, and data were tested for normality using the Kolmogorov–Smirnov test for distribution. Variables that conformed to a normal distribution were analyzed using unpaired t-tests. Results: The mean mandibular length in those with nutritional status lower than normal was 94.52 ± 5.89 mm, whereas it was 109.44 ± 4.85 mm in those with normal nutritional status. The results of an unpaired t-test (P = 0.001) revealed that there was a difference of mandibular length between subjects with normal nutrition status and lower than normal nutrition status. Conclusion: There was a relationship between nutritional status and mandibular length in subjects aged 10–16 years.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"144 - 147"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49008161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Root canal cleaning effectiveness with passive ultrasonic irrigation: A comparative in vitro study between Irrisafe and EndoUltra tip 被动超声冲洗根管清洁效果：Irrisafe和EndoUltra根尖的体外比较研究

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_65_21

Erlando Sartiono, Bernard O. Iskandar, Aryadi, W. Sudhana

{"title":"Root canal cleaning effectiveness with passive ultrasonic irrigation: A comparative in vitro study between Irrisafe and EndoUltra tip","authors":"Erlando Sartiono, Bernard O. Iskandar, Aryadi, W. Sudhana","doi":"10.4103/sdj.sdj_65_21","DOIUrl":"https://doi.org/10.4103/sdj.sdj_65_21","url":null,"abstract":"Background: Root canal irrigation has an important role in removing pulp tissue, dentine debris, and microorganisms in root canals. Passive ultrasonic irrigation (PUI) Irrisafe is a high-frequency wired ultrasonic device, which is usedto obtain cleanliness of root canal from cavitation and acoustic streaming. EndoUltra is a high-frequency wireless ultrasonic device. It has advantages in terms of its ease of application. However, further research is needed to identify the variations in effectiveness between PUI Irrisafe and EndoUltra tip for dentine debris cleanliness. Objective: The aim of this study was to determine the effectiveness between the activation of PUI Irrisafe and EndoUltra tip on the dentine debris cleanliness of the apical third of a root canal. Methods: This experimental study was carried out on 50 mandibular premolar teeth with a single root canal from extracted teeth. The teeth were irrigated with 5% NaOCl with the activation of EndoUltra # 20/02 (15 and 30 s), with 5% NaOCl with the activation of the PUI tip Irrisafe # 20/02 (15 and 30 s), or with conventional irrigation (30 s). The assessment of the dentine debris score was assessed by two observers using a scanning electron microscope (SEM). Analysis of the results used the Kruskal—Wallis and Mann—Whitney U tests. Results: Differences in median cleanliness scores for the apical third of the root canal from the Irrisafe group, the EndoUltra group, and conventional group had a Kruskal—Wallis test value of P = 0.005. The results showed that there were significant differences (P < 0.05) in five groups. Conclusion: PUI activation with the EndoUltra tip had a better effectiveness on the dentine debris cleanliness of the apical third of a root canal than the Irrisafe tip at 15 and 30 s.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"122 - 127"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45160048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Clinacanthus nutans leaf extract on oral mucosal burns and tongue wounds: An in-vivo study 胡桃叶提取物对口腔黏膜烧伤和舌头损伤的影响：体内研究

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_92_21

M. Roeslan, Salsabila Reskia, F. Firman, Hastania Dewa, K. Komariah, D. Bustami

{"title":"Effect of Clinacanthus nutans leaf extract on oral mucosal burns and tongue wounds: An in-vivo study","authors":"M. Roeslan, Salsabila Reskia, F. Firman, Hastania Dewa, K. Komariah, D. Bustami","doi":"10.4103/sdj.sdj_92_21","DOIUrl":"https://doi.org/10.4103/sdj.sdj_92_21","url":null,"abstract":"Background: Clinacanthus nutans exhibits antioxidant, anti-inflammatory, antiviral, anticancer, and anti-biofilm activities. Wounds in the oral cavity can affect the quality of life. Wound healing is a complex process to restore functions of injured tissues. The effect of C. nutans on oral mucosal burns and tongue wounds of rats is investigated. Methods: Forty male Sprague Dawley rats were divided into negative control and treatment groups with 25, 50, and 100 mg/mL of C. nutans. Oral mucosal burns and tongue wounds were observed on days 7 and 14 based on the fibroblast count and collagen deposits. Data were analyzed by analysis of variance and post hoc Tukey’s test with P < 0.05. Results: Results revealed that on day 7, the fibroblast count of mucosal burns and tongue wounds increases. The maximum mucosal burns were observed for the 100 mg/mL group, whereas the maximum tongue wounds were observed for the 25 mg/mL group. On day 7, collagen deposits were increased in case of mucosal burns. The number of collagen deposits increased on day 14, the highest mucosal burns were observed for the 100 mg/mL group, and in case of tongue wounds, the highest number was observed for the 50 mg/mL group. Conclusion: Ethanol extracts of C. nutans leaves can increase the fibroblast count on day 7 and collagen deposits on day 14 after injury in case of oral mucosal burns and tongue wounds during the wound healing of Sprague Dawley rats.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"101 - 109"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49582510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison between panoramic radiography and dental study model for space maintainer 全景x线摄影与口腔空间维持器研究模型的比较

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_82_21

M. Effendi, Berty Pramartika, Dini Rachmawati

{"title":"A comparison between panoramic radiography and dental study model for space maintainer","authors":"M. Effendi, Berty Pramartika, Dini Rachmawati","doi":"10.4103/sdj.sdj_82_21","DOIUrl":"https://doi.org/10.4103/sdj.sdj_82_21","url":null,"abstract":"Background: A space maintainer is an appliance used to maintain the jaw space due to the premature loss of deciduous teeth. Moyer’s method is used to determine the need for a space maintainer by measuring the discrepancy. Objective: The aim of this article is to determine the difference between measurements using Moyer’s method on a dental study model and on panoramic radiographs. Methods: Researchers measured the available space in 60 dental study models using two methods—the segmental method and contouring of the jaw arch using a 0.6 mm diameter arch wire. Moyer’s prediction table was used to predict the mesial-distal width of the permanent canines and premolars. Software ImageJ (SIJ) was used to measure the available space and required space on panoramic radiographs. We performed image calibration so that the measurement results could be presented in millimeters. Results: There was no significant difference (P = 0.935) in the discrepancy prediction between the panoramic radiographs and the dental study model. The Bland—Altman test result showed no significant difference, and the conformity between the panoramic radiographs and the dental study model was 0.02770. The sensitivity and specificity values of the panoramic radiographs and dental study model were above the 50% line. The area under curve values of the panoramic radiographs and dental study model were considered to be very good. Conclusion: Application of Moyer’s method to panoramic radiographs using SIJ to determine space maintainer requirements can be performed on pediatric patients to predict special discrepancies.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"115 - 121"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42443267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of garlic extract (Allium sativum L.) (Amaryllidaceae) to eradicate the Porphyromonas endodontalis biofilm: An in-vitro research 大蒜提取物(Allium sativum L.)根除牙髓卟啉单胞菌生物膜的体外研究

Scientific Dental Journal Pub Date : 2021-09-01 DOI: 10.4103/sdj.sdj_79_21

Adi Widjaya, M. Amin, Aryadi, B. Roeslan

{"title":"The effect of garlic extract (Allium sativum L.) (Amaryllidaceae) to eradicate the Porphyromonas endodontalis biofilm: An in-vitro research","authors":"Adi Widjaya, M. Amin, Aryadi, B. Roeslan","doi":"10.4103/sdj.sdj_79_21","DOIUrl":"https://doi.org/10.4103/sdj.sdj_79_21","url":null,"abstract":"Background: The Gram-negative bacterium Porphyromonas endodontalis is one of the causes of endodontic infections. Irrigation in root canal treatment was intended to eliminate bacteria. The garlic extract (Alium sativum Linnaeus) (Amaryllidaceae) is a natural material believed to have antimicrobial effects and may be used as an alternative irrigation solution. Objectives: The aim of this article is to evaluate the effect of garlic extract on the eradication of P. endodontalis biofilms. Methods: The A. sativum extract with concentrations of 100%, 50%, and 25%, NaOCl 5.25%, CHX 2% as a positive control, and sterile Aquadest as a negative control were used as irrigation solutions. A random sample of six groups was tested for P. endodontalis ATCC® 35406D-5TM biofilm eradication by irrigation of the A. sativum extract. Results: It was shown with a one-way analysis of variance that there was a significant difference in optical density (OD) between the results of biofilm eradication using A. sativum with concentrations of 100%, 50%, and 25% and NaOCl 5.25%, CHX 2%, and Aquadest (P < 0.05). With Tukey′s post hoc test, it was shown that the OD value from the eradication of A. sativum with concentrations of 100%, 50%, and 25% did not differ significantly compared with NaOCl 5.25% or CHX 2% (P > 0.05). Conclusion: The garlic extract (A. sativum) is effective in eradicating the biofilm of P. endodontalis in vitro.","PeriodicalId":32049,"journal":{"name":"Scientific Dental Journal","volume":"5 1","pages":"138 - 143"},"PeriodicalIF":0.0,"publicationDate":"2021-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41489611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1