Jurnal Teknologi Laboratorium最新文献_第5页

Conserved B-cell epitope identification of envelope glycoprotein (GP120) HIV-1 to develop multi-strain vaccine candidate through bioinformatics approach 应用生物信息学方法鉴定HIV-1包膜糖蛋白（GP120）的B细胞表位以开发多株候选疫苗

Jurnal Teknologi Laboratorium Pub Date : 2021-06-28 DOI: 10.29238/teknolabjournal.v10i1.274

V. D. Kharisma, A. Ansori, G. A. Posa, Wahyu Choirur Rizky, S. Permana, A. A. Parikesit

{"title":"Conserved B-cell epitope identification of envelope glycoprotein (GP120) HIV-1 to develop multi-strain vaccine candidate through bioinformatics approach","authors":"V. D. Kharisma, A. Ansori, G. A. Posa, Wahyu Choirur Rizky, S. Permana, A. A. Parikesit","doi":"10.29238/teknolabjournal.v10i1.274","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v10i1.274","url":null,"abstract":"Acquired immune deficiency syndrome (AIDS) has been identified from US patients since 1981. AIDS is caused by infection with the human immunodeficiency virus type 1 (HIV-1) which is a retrovirus. HIV-1 gp120 can be recognized by the immune system because it is located outside the virion. The conserved region is identified in gp120, and it is recognized by an immune cell which then initiates specific immune responses, viral mutation escape, and increase vaccine protection coverage, a benefit derived from the conserved region-based vaccine design. However, previous researchers have little knowledge on this conserved region as a target for vaccine design. This paper explains how the conserved region of gp120 HIV-1 is a major target for vaccine design through a bioinformatics approach. The conserved region from gp120 was explored as a vaccine design target with a bioinformatics tool that consists of B-cell epitope mapping, vaccine properties, molecular docking, and dynamic simulation. The peptide vaccine candidate of B5 with the gp120 HIV-1 conserved region was found to provoke B-cell activation through a direct pathway, produce specific antibody, and increase protection from multi-strain viral infection.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45448330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A simple method for isolation of rest of trypsinized stem cells with magnetic beads 磁珠分离胰蛋白酶化干细胞的一种简单方法

Jurnal Teknologi Laboratorium Pub Date : 2021-06-28 DOI: 10.29238/TEKNOLABJOURNAL.V10I1.268

Sudhir Bhatia

{"title":"A simple method for isolation of rest of trypsinized stem cells with magnetic beads","authors":"Sudhir Bhatia","doi":"10.29238/TEKNOLABJOURNAL.V10I1.268","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V10I1.268","url":null,"abstract":"Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collected. Magnetic beads are being used around the world to isolate different kinds of cells like CD4, CD8, CD34, and other cells. One of the advantages of magnetic isolation is that they can be used to isolate cells from solutions containing even a low number of targeted cells. We conducted the experiments to see whether all remaining MSC can be obtained from a trypsin-treated flask with magnetic beads isolation. \u0000During the magnetic isolation with CD90 specific Magnetic beads, it was found under the microscope that there are huge numbers of cells being attached to beads. The results indicate that there are still a huge number of rest cells in the trypsinized flask but most of the users think that they have isolated all cells, which is not true and they are throwing away valuable stem cells. The magnetic beads can be used to isolate the rest of MSC because for many applications, there is a need of the largest number of stem cells for conducting studies like flow cytometry, molecular testing along with pre-clinical and clinical studies.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45809366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Absolute leukocytes count and NLR as a diagnostic and prognostic biomarkers for severity of COVID-19 infection 绝对白细胞计数和NLR作为COVID-19感染严重程度的诊断和预后生物标志物

Jurnal Teknologi Laboratorium Pub Date : 2021-06-28 DOI: 10.29238/teknolabjournal.v10i1.263

Khalid Abdelsamea Mohamedahmed, A. Abakar

引用次数: 0

Detection of B1 gene as Toxoplasmosis marker in women of childbearing age in West Bandung Regency, Indonesia 印度尼西亚西万隆县育龄妇女弓形虫病标志物B1基因的检测

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.204

Patricia Gita Naully, Selvi Anggraeni Supendi

{"title":"Detection of B1 gene as Toxoplasmosis marker in women of childbearing age in West Bandung Regency, Indonesia","authors":"Patricia Gita Naully, Selvi Anggraeni Supendi","doi":"10.29238/TEKNOLABJOURNAL.V9I2.204","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.204","url":null,"abstract":"Congenital toxoplasmosis can cause damage and death to the fetus, to prevent this case, toxoplasmosis testing is important for the woman of childbearing age. One of the methods to screening the presence of T. gondii in the blood is Polymerase Chain Reaction (PCR). One of the T. gondii genes which can be used as a marker is the B1 gene. There are many toxoplasmosis cases in Indonesia, but the data is still difficult to find in West Bandung Regency. This study aimed to determine the number of toxoplasmosis cases in a woman of childbearing age in West Bandung Regency using the B1 gene as a marker and to determine the factors that influence these cases by conducting statistical analysis on the results of the questionnaire. The sample used in this study was 50 women of childbearing age (got married and domiciled in West Bandung). All samples have met the inclusion criteria and signed the informed consent. DNA from blood specimens was isolated using the Wizard Genomic DNA Purification Kit. The concentration and purity of isolated DNA were measured using a nanodrop device. Besides, the B1 gene from T. gondii was amplified using a pair of specific primers and visualized by the agarose electrophoresis method. Data were analyzed using the logistic regression method. The results showed that 7 women of childbearing age women (14%) in West Bandung Regency had toxoplasmosis. Frequent contact with pets, especially cats, was a significant factor (p <0.005) in this disease transmission.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42028719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method 用STR PCR方法研究温度和保存时间对FGA和D13S317基因座的影响

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.243

I. Masjkur, Evy Arfianti, A. Yudianto, A. Furqoni, Q. A’yun

{"title":"The effect of temperature and storage time of cuccal swabs on FGA and D13S317 loci with the STR PCR method","authors":"I. Masjkur, Evy Arfianti, A. Yudianto, A. Furqoni, Q. A’yun","doi":"10.29238/TEKNOLABJOURNAL.V9I2.243","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.243","url":null,"abstract":"The samples used for forensic DNA analysis in living individuals are usually blood and buccal swabs, however, blood collection requires an invasive method that can cause discomfort, thus a buccal swab can be a good choice for individuals examined, especially children. This study aimed to determine the effect of temperature and storage time of buccal swabs on the quantity of DNA as material for DNA examination in the forensic field. This study was a laboratory experiment to determine the effect after treatment. Buccal swab samples were 48 and divided into 2 temperature groups, namely room temperature (RT) and 4℃. The division of the temperature groups was also observed with time differences, namely 1, 3, 5, 7 days. EDNA extraction used the DNAzol method and DNA quantification used a Spectrophotometer. The PCR process was carried out with STR primers FGA and D13S317 loci. The visualization stage used acrylamide gel and silver staining. \u0000The results of this study prove that there is an effect of temperature and storage time of buccal swab samples. The longer the treatment time, the lower the DNA level. With statistical analysis, it is obtained p-value of <0.005, it can be concluded that there are significant differences in DNA levels at the temperature and storage time treatments of the buccal swab sample. The results of DNA visualization at the FGA and D13S317 loci using the STR PCR method in this study can still be detected and can be used as a reference for examination in forensic cases. \u0000 ","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46544836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA) 三元石竹种子提取物对耐甲氧西林金黄色葡萄球菌(MRSA)的体外抗菌活性

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.237

M. E. Prastiyanto, I. H. Azizah, Hafizha Dara Haqi, Bagus Yulianto, A. B. Agmala, Zhafira Dias Radipasari, Nita Ayu Dwi Astuti, Arifani Rahma Putri

{"title":"In-vitro antibacterial activity of the seed extract of three-member Artocarpus towards Methicillin-Resistant Staphylococcus aureus (MRSA)","authors":"M. E. Prastiyanto, I. H. Azizah, Hafizha Dara Haqi, Bagus Yulianto, A. B. Agmala, Zhafira Dias Radipasari, Nita Ayu Dwi Astuti, Arifani Rahma Putri","doi":"10.29238/TEKNOLABJOURNAL.V9I2.237","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.237","url":null,"abstract":"Methicillin-Resistant Staphylococcus aureus (MRSA) infections have created a critical need for the development of natural antibacterials from a biological source. This research aimed to investigate the antibacterial activity of the seed extract of three-member Artocarpus (Artocarpus heterophyllus, A. champeden, and A. camansi) against MRSA which are the most prevalent causes of infections in patients. Crude seed extracts of three-member Artocarpus were evaluated for their antibacterial activity against MRSA. The antibacterial activity against MRSA of the three extracts was assayed in vitro by the agar well diffusion assay and agar microdilution method and minimum bactericidal concentration. The antibacterial activity, calculated as a zone of inhibition and MIC, MBC values. The Crude seed extracts of three-member Artocarpus showed antibacterial activity against the MRSA in the agar well diffusion assay (1.5-9 mm inhibition diameter). The MIC value of extract showed at 15.62 mg/mL and the MBC value of seed extract of A. heterophyllus at 62.5 mg/mL, A. champeden at 31.25 mg/mL, A. camansi at 250 mg/mL. All seed extracts have the potential to be developed as antibacterial agents, particularly against MRSA strain. Studies on the antibacterial activity against MRSA can provide new information about the benefits seed of members of Artocarpus as a source of natural antibacterial.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43446109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia 印尼三宝垄Tambaklorok接触铅的居民红细胞指数

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.213

B. Santosa, Herlisa Anggraini, Ika Mawarni, Niza Muliatun, Ardiansyah Nugroho, Uly Syarah, Siti Atika Risqoeni

{"title":"Erythrocyte index of residents exposed to lead in Tambaklorok, Semarang, Indonesia","authors":"B. Santosa, Herlisa Anggraini, Ika Mawarni, Niza Muliatun, Ardiansyah Nugroho, Uly Syarah, Siti Atika Risqoeni","doi":"10.29238/TEKNOLABJOURNAL.V9I2.213","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.213","url":null,"abstract":"Erythrocyte index can form erythrocyte morphology in the peripheral blood smear preparation. Lead exposure in Tambaklorok exceeds the threshold that affects anemia. The purpose of this study was to determine the erythrocyte index confirmation with erythrocyte morphology in the peripheral blood smear preparation. A cross-sectional study was conducted in 2month in Tambaklorok Semarang residents and 104 samples were taken using the purposive technique. Erythrocyte index was measured using the hematological analyzer and erythrocyte morphology in the peripheral blood smear preparation using Giemsa painting. Erythrocyte index confirmation with the peripheral blood smear preparation was analyzed using the Gamma statistical relationship test. The results showed that the erythrocyte index value was mostly in the normal category, i.e., MCH 68 (64.4%), MCHC 61 (58.6%) and MCH 58 (56%) and below normal category were MCH 45 (42.95%), MCHC 41 (39.4%), and MCV 36 (34.3%). Erythrocyte morphology was mostly hypochromic, namely 46 (44.23%), normochrome 40 (38.46%), and hyperchrome 18 (17.3%). Relationship of MCH with erythrocyte color p-value 0.037 with size p-value 0.038. Conclusion of erythrocyte index confirmation, especially MCH with the peripheral blood smear preparation, there was a match on the color and size of erythrocytes, while the MCV and MCHC values had no significant relationship.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42387024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Peripheral stem cell mobilization strategies in patients with autologous hematopoietic cell transplantation: A single center's experience 自体造血细胞移植患者外周血干细胞动员策略:单一中心的经验

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.247

S. Namdaroğlu, T. Yiğenoğlu, H. Batgi, B. Ulu, D. İskender, M. Çakar, M. Dal, F. Altuntaş

{"title":"Peripheral stem cell mobilization strategies in patients with autologous hematopoietic cell transplantation: A single center's experience","authors":"S. Namdaroğlu, T. Yiğenoğlu, H. Batgi, B. Ulu, D. İskender, M. Çakar, M. Dal, F. Altuntaş","doi":"10.29238/TEKNOLABJOURNAL.V9I2.247","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.247","url":null,"abstract":"This research is to investigate the parameters which may affect the mobilization of stem cells in patients receiving autologous hematopoietic peripheral blood stem cell transplantation (PBSCT). A retrospective study was carried out using the data derived from the medical files of 242 patients who received PBSCT. Descriptive, clinical, and laboratory parameters were compared between patients with successful and unsuccessful stem cell mobilization. Successful stem cell mobilization ratio was 4.463 times higher when preemptive plerixafor was administrated; 1.032 times higher when CD34+ cell count increased 1 unit at the beginning of mobilization. The white blood cell count was inversely correlated with the success of mobilization. An increase of 1 unit in WBC count was associated with a 1.027 times decrease in the success rate. The data indicated that the administration of preemptive plerixafor and CD34+ cell count at the beginning of mobilization were directly related to the success of mobilization after PBSCT. On contrary, WBC count was inversely associated with the success rate.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45132258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial 白石榴叶乙醇提取物的抑菌潜力

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.258

N. Susilawati, I. G. P. Arnawa, Karol Octrisdey, N. Kambuno

{"title":"The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial","authors":"N. Susilawati, I. G. P. Arnawa, Karol Octrisdey, N. Kambuno","doi":"10.29238/TEKNOLABJOURNAL.V9I2.258","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.258","url":null,"abstract":"Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL. \u0000 ","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42310318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L 苦参甲醇提取物角鲨烯化合物的抗炎活性研究

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI: 10.29238/TEKNOLABJOURNAL.V9I2.228

M. Latief, M. Muhaimin, H. Amanda, Graha Prahandika, I. L. Tarigan

{"title":"Anti-inflammatory activities of squalene compound of methanol extract of Abroma augusta L","authors":"M. Latief, M. Muhaimin, H. Amanda, Graha Prahandika, I. L. Tarigan","doi":"10.29238/TEKNOLABJOURNAL.V9I2.228","DOIUrl":"https://doi.org/10.29238/TEKNOLABJOURNAL.V9I2.228","url":null,"abstract":"Abroma augusta L plant traditionally was used to treat swellings, cuts, sores, and bruises. In the province of Jambi, A. augusta is used in folk medicine to treat wounds. This study aims to isolate the steroid compound from the root of A. augusta L and determine its anti-inflammatory activities. Extraction and fractionation have been done with graded maceration using solvents with different polarities, which are n-hexane, ethyl acetate, and methanol. The separation was performed by column chromatography, followed by preparative thin-layer chromatography. The characterization of the isolate was carried out using UV-Vis spectrophotometry and infrared spectrophotometry, GC-MS. The anti-inflammatory activities of methanol extract and isolate of A. augusta was performed in this study was designed to evaluate the dose-response relationship of the anti-inflammatory activity in rat models of chronic inflammation chromatography to obtain isolate 2.1.1 that characterize and showed maximum absorbance at 265. The result of IR showed the presence of functional groups, -C=C-H, -C=H, -CH, CH3, CH2, and –CO belongs to the steroid compound. The results of the GC-MS show that isolates contain squalene compounds with a value of m/z 410, Isolate and crude extract showed an anti-inflammatory activity that almost approached the positive control of sodium 4-chlorophenolate. It could be concluded that isolate and extract provide good anti-inflammatory activity, that promise for new drug candidate squalene-based A. augusta.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42958782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1