The potential of ethanol extract of white pomegranate leaves (Punica granatum L) as anti-bacterial

Jurnal Teknologi Laboratorium Pub Date : 2020-12-31 DOI:10.29238/TEKNOLABJOURNAL.V9I2.258

N. Susilawati, I. G. P. Arnawa, Karol Octrisdey, N. Kambuno

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引用次数: 2

Abstract

Treatment of infections using penicillin-derived antibiotics such as methicillin has been found to cause antibiotic-resistant bacteria. This bacteria could produce a beta-lactamase enzyme to form a resistant strain. Research on antibacterial activity continues to develop. Pomegranate (Punica granatum L.) was one of the herbal plants whose fruit has long been used for the treatment and prevention of various diseases. This study aimed to determine the potential inhibition of white pomegranate leaf extracts (Punica granatum L.) on the growth of Gram-negative bacteria including Escherichia coli Extended-Spectrum Beta-Lactamase (ESBL) strain and Gram-positive bacteria Staphylococcus aureus Methicillin-Resistant Staphylococcus aureus (MRSA) strain. White pomegranate leaf extract macerated with ethanol 96%, evaporated to obtain pure extracts made with a concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% and with 100% tested with invitro diffusion method. It was found that the extract of white pomegranate leaves with 30% (10.00 ± 0.0) concentration was able to inhibit the growth of positive Gram bacteria strains MRSA and the extract was unable to inhibit the growth of Escherichia coli bacteria strain ESBL.

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白石榴叶乙醇提取物的抑菌潜力

使用青霉素衍生的抗生素如甲氧西林治疗感染已被发现会导致抗生素耐药性细菌。这种细菌可以产生一种β-内酰胺酶，形成耐药菌株。抗菌活性的研究仍在继续发展。石榴（Punica granatum L.）是一种草本植物，其果实长期用于治疗和预防各种疾病。本研究旨在确定白石榴叶提取物（Punica granatum L.）对革兰氏阴性菌（包括大肠杆菌扩展谱β-内酰胺酶（ESBL）菌株和革兰氏阳性菌金黄色葡萄球菌耐甲氧西林金黄色葡萄菌（MRSA）菌株）生长的潜在抑制作用。白石榴叶提取物用96%乙醇浸渍，蒸发得到浓度分别为10%、20%、30%、40%、50%、60%、70%、80%、90%和100%的纯提取物。结果表明，30%（10.00±0.0）浓度的白石榴叶提取物能抑制革兰氏阳性菌MRSA的生长，而提取物不能抑制大肠杆菌ESBL的生长。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Jurnal Teknologi Laboratorium

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审稿时长

24 weeks