Lekovite Sirovine最新文献

Evaluation of antioxidant activities and haematological effects of Asystasia gangetica leaf extract in monosodium glutamate-treated rats 钩藤叶提取物对味精处理大鼠抗氧化活性和血液学作用的评价

Lekovite Sirovine Pub Date : 2021-09-03 DOI: 10.5937/LEKSIR2141005U

Robert Ikechukwu Uroko, A. Agbafor, S. Egba, C. Nwuke, Sharon Nnenne Kalu-Kalu

{"title":"Evaluation of antioxidant activities and haematological effects of Asystasia gangetica leaf extract in monosodium glutamate-treated rats","authors":"Robert Ikechukwu Uroko, A. Agbafor, S. Egba, C. Nwuke, Sharon Nnenne Kalu-Kalu","doi":"10.5937/LEKSIR2141005U","DOIUrl":"https://doi.org/10.5937/LEKSIR2141005U","url":null,"abstract":"This study evaluated antioxidant activities and haematological effects of methanol extract of Asystasia gangetica leaves (MEAG) on monosodium glutamate (MSG) treated rats. Forty-two male Wistar rats randomly distributed into 7 groups (n=6) were used for the study. Groups 1-3 were the normal control, MSG control, and positive control respectively whereas groups 4-5 were the extract controls, and groups 6-7 were the curative groups. All the treatments were administered orally and standard analytical methods were used for analyses. The results showed that MEAG is a rich antioxidants source and contains phenolics, flavonoids and beta-carotene in high concentrations. The extract showed concentration-dependent increases in ferric reducing antioxidant power, DPPH (2,2-diphenyl-1-picrylhydrazyl) and nitric oxide radicals scavenging activities but relatively lower than their respective controls. The MSG administration caused significant (P<0.05) reductions in the glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT) activities but significantly (P<0.05) increased the malondialdehyde (MDA) concentrations in the MSG control rats. The MSG administration also caused significant (P<0.05) reductions in the haemoglobin (Hb) concentration, packed cell volume (PCV), red blood cell (RBC) and white blood cell (WBC) counts of the MSG control relative to the normal control. Treatment with MEAG significantly elevated GPx, SOD, and CAT activities; haematological indices and significantly reduced MDA levels in the extract controls, and curative groups treated with a high dose of MSG. These findings show that methanol extract of A. gangetica leaves is rich in antioxidants that could prevent oxidative stress and improves the haematological profile of MSG treated rats.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41824967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Qualitative and quantitative phytochemical evaluation of Quassia undulata (Guill. & Perr.) D. Dietr. leaves using different solvent polarities 波纹桂的定性和定量植物化学评价。使用不同溶剂极性的叶子

Lekovite Sirovine Pub Date : 2021-09-03 DOI: 10.5937/LEKSIR2141013L

L. Oduola, Adeniyi Olufunso, Ariwoola Oluwole

{"title":"Qualitative and quantitative phytochemical evaluation of Quassia undulata (Guill. & Perr.) D. Dietr. leaves using different solvent polarities","authors":"L. Oduola, Adeniyi Olufunso, Ariwoola Oluwole","doi":"10.5937/LEKSIR2141013L","DOIUrl":"https://doi.org/10.5937/LEKSIR2141013L","url":null,"abstract":"This study investigated solvent effects on the phytochemical composition of Quassia undulata leaves a medicinal plant used in treating arrays of diseases including fever and cough. The leaves were collected, washed, air-dried, pulverized and evaluated for some inherent phytochemicals using four different solvent systems based on their polarities. The solvents are methanol, acetone, ethyl acetate and chloroform. The methanol extract was found to have the highest number of secondary metabolites (saponins, tannins, flavonoids, steroids, coumarins, anthraquinones, alkaloids and phenols). None of the extracts tested positive for the presence of phlobatannins, terpenoids and emodins. The methanol extract was further analyzed quantitatively for some of the determined phytochemicals. Tannins had a concentration of 3.131 mg of catechin equivalents per 100 mg sample (mg CE/100g), alkaloids-5.200 %, total phenolics-11.828 mg of gallic acid equivalents per gram of extract (mg GAE/g), flavonoids-8.074 mg of quercetin equivalents per gram of extract (mg QE/g) while 0.673 % saponins were detected. The presence of these secondary metabolites might justify the ethnomedicinal uses of Quassia undulata leaves as their bioactivity has been found to be dependent on the solvent used for extraction.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48287479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antifungal activity of the essential oil from Artemisia santonicum and its constituent isogeranic acid 山蒿精油及其成分异香叶酸的抗真菌活性

Lekovite Sirovine Pub Date : 2020-12-25 DOI: 10.5937/LEKSIR2040062S

Jovana Stankovic-Jeremic, M. Novaković, V. Tešević, A. Ćirić, G. Zdunić, Z. Dajić-Stevanović, D. Gođevac

引用次数: 1

Phyto-pharmacological aspects of Nepeta nuda L.: A systematic review 荆芥植物药理学研究综述

Lekovite Sirovine Pub Date : 2020-12-16 DOI: 10.5937/LEKSIR2040075A

M. Aćimović, Jovana Stankovic-Jeremic, M. Cvetković

引用次数: 5

Development of gastroretentive floating granules with gentian root extract by hot-melt granulation 龙胆根提取物热熔造粒法制备保胃漂浮颗粒

Lekovite Sirovine Pub Date : 2020-12-12 DOI: 10.5937/LEKSIR2040040M

J. Mudrić, M. Pajić, Jelena Đuriš

{"title":"Development of gastroretentive floating granules with gentian root extract by hot-melt granulation","authors":"J. Mudrić, M. Pajić, Jelena Đuriš","doi":"10.5937/LEKSIR2040040M","DOIUrl":"https://doi.org/10.5937/LEKSIR2040040M","url":null,"abstract":"The roots of yellow gentian, Gentiana lutea L. (Gentianaceae) are used in traditional medicine for the treatment of gastrointestinal disorders, with the literature data indicates a local gastric effect of gentian root extract (GRE) and support the use of the solid pharmaceutical forms. Gentiopicroside, as a dominant secoiridoid in the GRE, has a short elimination half-life and low bioavailability and, consequently, its bioactivity is limited. The aim of the study was to develop gastroretentive floating delivery system with GRE, and to provide prolonged release of gentiopicroside. Floating granules with dry GRE (DGRE) were prepared by the hot-melt granulation technique, while formulations included effervescent components (citric acid and sodium bicarbonate), hydroxypropyl methylcellulose (HPMC) and meltable binders (Compritol® 888 ATO and Gelucire® 50/13). The flowability of the DGRE and prepared formulations was determined by calculating the Carr index and Hausner ratio. Floating properties and in vitro dissolution rate of gentiopicroside from investigated formulations were examined. Floating granules were characterized with improved flowability (Carr index 14-22 %; Hausner ratio 1.16-1.28) in comparison to the DGRE (Carr index 28.99 %; Hausner ratio of 1.41). Furthermore, the floating granules exhibited immediate and long-lasting buoyancy and prolonged-release of gentiopicroside (over 8 h). Compritol® 888 ATO has provided sustained release of gentiopicroside from floating granules, while HPMC has decreased release rate additionally. On the other hand, Gelucire® 50/13 has increased gentiopicroside release rate. The results have shown that hot-melt granulation technique, as a green granulation method was successfully employed for obtaining gastroretentive floating granules with DGRE.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46256894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Double emulsions (W/O/W emulsions): Encapsulation of plant bioactives 双乳液（W/O/W乳液）：植物生物活性物质的封装

Lekovite Sirovine Pub Date : 2019-12-18 DOI: 10.5937/leksir1939076m

J. Mudrić, K. Šavikin, S. Ibrić, Jelena Đuriš

引用次数: 5

Exploring the attitude of Faculty of Medicine students about herbal products usage: A cross-sectional study 探讨医学院学生对草药产品使用的态度:一项横断面研究

Lekovite Sirovine Pub Date : 2019-12-18 DOI: 10.5937/leksir1939069k

E. Kostić, Milica Milutinović, Milica Kostić, J. Petrović, Bojana Miladinović, S. Branković, M. Vujović, D. Kitić

{"title":"Exploring the attitude of Faculty of Medicine students about herbal products usage: A cross-sectional study","authors":"E. Kostić, Milica Milutinović, Milica Kostić, J. Petrović, Bojana Miladinović, S. Branković, M. Vujović, D. Kitić","doi":"10.5937/leksir1939069k","DOIUrl":"https://doi.org/10.5937/leksir1939069k","url":null,"abstract":"Herbal products have gained popularity, and they are often perceived as safe. The roles of the pharmacists and physicians are essential in the proper usage of those products. This study aimed to investigate the students of Faculty of Medicine in Nis about the use of herbal preparations and the differences between medicine, pharmacy and dentistry students. The fifth-year university students were questioned through an anonymous survey. The obtained results were processed statistically and presented graphically. The majority of the students would prefer a combination of herb product and drug (76.9%). Usage of herbal products is based on students' knowledge, on the advice of pharmacists, family members, physicians, and the media. The majority of students purchase herbal products in the pharmacy and nobody obtained them via the Internet. Most of the students used them in preventive purposes (60%) and claimed that there were positive effects. Students (75%) read the instructions for use and side effects, especially pharmacy students (88.3%). Medicine students (60%) consider herbal products completely safe. Pharmacy students are considered to receive the necessary knowledge on herbal products (68.3%), while a smaller percentage of medicine and dentistry students consider the same. Students use herbal medicines but are not entirely familiar with their effects and safety. There is a need for raising awareness, the importance of side effects and interactions of herbs and drugs among medicine and dentistry students. This goal can be achieved by changing curriculum which would contain material from phytotherapy.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42918368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro evaluation of antioxidant, antineurodegenerative and antidiabetic activities of Ocimum basilicum L., Laurus nobilis L. leaves and Citrus reticulata Blanco peel extracts 芫荽叶、月桂叶和柑桔皮提取物抗氧化、抗神经退行性和抗糖尿病活性的体外评价

Lekovite Sirovine Pub Date : 2019-12-17 DOI: 10.5937/leksir1939060d

S. Duletić-Laušević, Mariana M Oalđe, Ana Alimpic-Aradski

{"title":"In vitro evaluation of antioxidant, antineurodegenerative and antidiabetic activities of Ocimum basilicum L., Laurus nobilis L. leaves and Citrus reticulata Blanco peel extracts","authors":"S. Duletić-Laušević, Mariana M Oalđe, Ana Alimpic-Aradski","doi":"10.5937/leksir1939060d","DOIUrl":"https://doi.org/10.5937/leksir1939060d","url":null,"abstract":"Ocimum basilicum (sweet basil) and Laurus nobilis (bay leaves or laurel) have been used in traditional medicine for centuries, and also extensively employed as spices for adding aroma and flavor to various food products. Citrus reticulata (mandarin) is mainly used in food industry for juice production, while its peel as main byproduct contains high concentration of valuable substances. The samples were collected in Lastva Grbaljska (Montenegrin coast) and purchased from the market. Since the oxidative stress results in development of numerous diseases, among them neurodegeneration and diabetes, the antioxidant activity, antineurodegenerative and antidiabetic activities were analyzed, aiming to compare potential of plants cultivated under natural conditions and commercially purchased from the market, as well as to compare the effect of different solvents applied in the extraction process. Water, methanol and acetone extracts of leaves and peel were tested by DPPH and total reducing power (TRP) methods for determination of antioxidant activity, and by acetylcholinesterase (AChE) and α-glucosidase inhibition assays for analyzing the other activities. Total phenolic (TPC) and flavonoid (TFC) contents were also determined. The acetonic extract of L. nobilis from Lastva showed the highest TPC, DPPH, TRP, and α-glucosidase inhibition, while water extract of commercial L. nobilis exhibited the highest AChE inhibition. The leaves of L. nobilis are demonstrated to be promising antioxidant, antineurodegenerative and antidiabetic agent.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47635705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Investigation of stability of cold pressed linseed (Linum usitatissimum L.) oil 冷压亚麻籽油稳定性的研究

Lekovite Sirovine Pub Date : 2019-12-09 DOI: 10.5937/leksir1939040d

Z. Drinić, J. Mudrić, G. Zdunić, Dubravka Bigović, K. Šavikin

引用次数: 0

Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity 乙醇胸腺提取物：通过总多酚含量和自由基清除活性评价提取条件

Lekovite Sirovine Pub Date : 2019-11-29 DOI: 10.5937/leksir1939023j

A. Jovanović, M. Skrt, P. Petrović, I. J. Častvan, G. Zdunić, K. Šavikin, B. Bugarski

{"title":"Ethanol Thymus serpyllum extracts: Evaluation of extraction conditions via total polyphenol content and radical scavenging activity","authors":"A. Jovanović, M. Skrt, P. Petrović, I. J. Častvan, G. Zdunić, K. Šavikin, B. Bugarski","doi":"10.5937/leksir1939023j","DOIUrl":"https://doi.org/10.5937/leksir1939023j","url":null,"abstract":"The aim of the present study was the optimization of the extraction conditions (particle size, solid-to-solvent ratio, extraction time and procedure) via total polyphenol content and radical scavenging activity, using wild thyme as a natural source of polyphenols. According to the total polyphenols yield, particle size, solvent-to-solid ratio and extraction technique have shown statistically significant influence, whereas the exposure time has not been relevant factor. In all three extraction procedures (maceration, heat- and ultrasound-assisted extraction), the highest polyphenols yield was reached using the smallest plant particles and the highest solvent-to-solid ratio. Moreover, polyphenols content was significantly better after ultrasound-assisted extraction, in comparison to the extraction at room and high temperature. According to the antioxidant recovery, the influence of different extraction parameters varied depending on the applied antioxidant tests and extraction procedures. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ˗ ABTS radical scavenging capacity of extracts followed the next trend: maceration and ultrasound-assisted extraction > heat-assisted extraction, while in 2,2-diphenyl-1-picrylhydrazyl ˗ DPPH neutralization: ultrasound-assisted extraction > heat-assisted extraction >> maceration. The study represents an important step in formulation of antioxidants-rich extracts with potential use in food, pharmaceutical and cosmetic industry.","PeriodicalId":31829,"journal":{"name":"Lekovite Sirovine","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42316819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0