{"title":"Ethanol extract of soursop leaves inhibits acid production and adhesion of Streptococcus mutans","authors":"Friska Ani Rahman, Rahsunji Intan","doi":"10.24198/pjd.vol33no3.29385","DOIUrl":"https://doi.org/10.24198/pjd.vol33no3.29385","url":null,"abstract":"ABSTRACTIntroduction: Dental caries and dental plaque are the most popular global oral health problems. The primary step of dental caries is characterized by damage of tooth surfaces affected by acids which are by-products of sugar metabolism by a cariogenic bacteria. One of the cariogenic bacteria is Streptococcus mutans. Annona muricata is traditionally applied as an herbal remedy for various illnesses and has been recognized in a previous study as an antimicrobial agent. The aim of this study was to investigate acid production and adhesion of ethanol extract of Soursop on Streptococcus mutans. Methods: Soursop leaf extracted by maceration using 70% ethanol solvent. The extracts obtained were tested at various concentrations. To examine the effect of ethanol extract of Soursop on acid production by S. mutans, the pH of the culture was determined using a pH meter. Inhibition of adhesion of S. mutans to the saliva-coated hydroxyapatite (S-HA) discs was quantified using colony counting on TYS20B agar plates. Results: The pH of S. mutans cultures in the presence of ethanol extract of Soursop leaf at various concentrations was higher than negative control, but there were no differences in pH value between the various concentrations of ethanol extract of Soursop leaf. Adhesion of S. mutans to S-HA discs was inhibited by various concentrations of ethanol extract of Soursop leaf. Adhesion decreased with increasing concentrations of ethanol extract of Soursop leaf, but there was not significant difference in colony count between the various concentrations of ethanol extract of Soursop leaf. Conclusions: Ethanol extract of Soursop leaf attenuates the acid production and adhesion of S. mutans to hydroxyapatite discs. Keywords: Soursop (Annona muricata); Streptococcus mutans; acid production; adhesion","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45595619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fania Syakira Susilo, Dudi Aripin, A. A. Suwargiani
{"title":"Practices of oral health maintenance, caries protective factors and caries experience in adults in Sekeloa Region","authors":"Fania Syakira Susilo, Dudi Aripin, A. A. Suwargiani","doi":"10.24198/pjd.vol33no2.28419","DOIUrl":"https://doi.org/10.24198/pjd.vol33no2.28419","url":null,"abstract":"Introduction: Dental caries is still the most common oral disease experienced in Indonesia. Caries is caused by various factors. Practices in maintaining proper oral health are urgently needed to maintain healthy teeth and mouth to avoid various diseases, including caries. Caries prevention is inseparable from the role of protective factors that maintain the caries balance. This study was aimed at discovering the level of oral health maintenance practices, caries protective factors, as well as caries experience in adults in Sekeloa Region. Methods: The research method was descriptive cross-sectional with purposive sampling technique. The sample consisted of 61 permanent residents aged 35-44 years in South Sekeloa. Data was collected through questionnaire and DMF-T index examination. Discussion: Dental health maintenance practices including frequency, times, duration, techniques, type of brush bristles, use of brushes according to its lifetime, use of fluoride-containing toothpaste, use of interdental cleaning tools, frequency of dental visits, frequency of scaling procedures, and food consumption along with caries protective factors including the consumption of non-cariogenic snacks, xylitol chewing gum, use of chlorhexidine or fluoride mouthwash, pit and fissure sealant and topical fluoride treatments play an important role in preventing caries. Results: This study shows that subjects performed good practices of oral health maintenance (47.54%), adequate (50.82%), bad (1.64%), and good caries protective factors (1.64%), adequate (52.46%), bad (45.90%). The caries experience degree reached 9.13 and was categorized as moderate. Conclusion: The degree of caries experience in adults aged 35-44 years in the Sekeloa Region was moderate, oral health maintenance practices and caries protective factors were adequate. ","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45165927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of different fluoride varnishes on the release of calcium ions from hydroxyapatite discs: An ion-selective electrodes study","authors":"Aimi Kamarudin, P. Anderson, R. Hill","doi":"10.24198/pjd.vol32no2.26444","DOIUrl":"https://doi.org/10.24198/pjd.vol32no2.26444","url":null,"abstract":"Introduction: Recently, various modes of fluoride varnishes have evolved, each with its own recommended concentration, potentially active ingredients, and flavour, leading to a claim of additional preventive benefits. Differences in fluoride release patterns can potentially enhance or reduce the efficacy of fluoride varnishes. Numerous clinical trials have proven its ability in preventing and arresting dental caries. This study mainly focused on the investigations of the apatite demineralisation process under the effect of different fluoride varnishes by ion-selective electrodes (ISE), in an attempt to comprehend their mechanism in anti-caries. Methods: Four different fluoride varnishes (Fluor Protector S, Duraphat, ClinPro White, MI Varnish) were used to measure their effect on the demineralisation process of the hydroxyapatite (HAP) discs in 60ml pH 4.0 acetic solutions. The HAP discs were treated with these varnishes after 4-hours demineralisation and then immersed back into the same solutions for further demineralisation to observe the effect of the varnishes. Throughout the experiment, the calcium ISE was used to monitor the rate of calcium concentration. Results: The result demonstrated that ClinPro White varnish resulted in the most significant inhibition of demineralisation and signs of probable remineralisation throughout the experiment. Other fluoride varnishes treatment showed the ability to inhibit demineralisation. However, the rate of calcium dissolution was not significantly different from different varnishes. The fact that the ClinPro White showed evidence of remineralisation might be associated with the fact that the varnish contained a source of calcium and phosphate. Conclusion: The fluoride varnishes treatment is shown to be effective in inhibiting the demineralisation of apatite regardless of the difference in fluoride concentration and potentially active ingredients incorporated in some of the fluoride varnishes.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42204132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of nasopharynx area and level of severity posterior crossbite on children with cleft lips and palate post-palatoplasty","authors":"Anie Apriani, R. Primarti, A. Kasim, F. Oscandar","doi":"10.24198/pjd.vol32no2.17951","DOIUrl":"https://doi.org/10.24198/pjd.vol32no2.17951","url":null,"abstract":"Introduction: Many children with post palatoplasty had crossbite posterior. This study was aimed to assess the nasopharynx area and the posterior crossbite severity level of children with cleft lip and palate (CLP) who received palatoplasty treatment compared to normal children. Methods: The study was observational analytic. The research subject was 14 children with CLP post-palatoplasty and 14 normal children. The object of research was 28 study models and secondary data of lateral cephalometric radiograph of children with CLP post palatoplasty and normal children. The measurement of PTM-ad1-Ad2-PTM and PTM-So-Ba-PTM were used to measure the nasopharyngeal area. Study models were assessed to analyse the level of severity of posterior crossbite. Results: The average of the soft tissues (the nasopharynx) area children with CLP post-palatoplasty was 35.02 mm2, which was lower than the normal child (35.73 mm2). Similarly, the average of the hard tissues (the nasopharynx) area children with CLP post-palatoplasty was 301.40 mm2, which was smaller than the normal children (315.54 mm2). Statistical analysis of the nasopharynx area resulted in non-significant difference. All children with CLP post-palatoplasty was suffered from posterior crossbite. The level of severity posterior crossbite, which was categorised as good was 42.9%, poor criteria was 35.7%, moderate criteria was 14.3%, and very good criteria was 7.1%. Conclusion: There is no difference between the average size of the nasopharynx area on children with CLP post-palatoplasty and normal children. The level of severity posterior crossbite after palatoplasty in CLP children mostly included in the good criteria.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45034889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cucu Zubaedah, P. Rufaidah, Dudi Aripin, S. Tjahajawati
{"title":"Women’s customer trusts in hospital's inpatient services","authors":"Cucu Zubaedah, P. Rufaidah, Dudi Aripin, S. Tjahajawati","doi":"10.24198/pjd.vol31no3.25135","DOIUrl":"https://doi.org/10.24198/pjd.vol31no3.25135","url":null,"abstract":"Introduction: Customer trust is a tribute to officers or institutions performances and sacrifices. In the health marketing system, the relationship among health workers, doctors, patients or health service users is essential. The declining of women’s health will have an impact on the overall life qualities of the community and eventually will decrease the Indonesian Human Development Index. The purpose of this research was to analyze the customer trust of women’s inpatient in hospitals at West Java and determine the most influential dimensions and indicators of the women’s inpatient customer trust. Methods: This research was explanatory research conducted with the survey method with the sample size of 398 respondents. The unit of analysis was a hospital in West Java, while the observation unit was the inpatient community group which considers inadequate and gets health financing subsidies from the government in 42 hospitals from 28 cities or regencies in the West Java provinces. The sampling method was multistage sampling and the research tool was a questionnaire. Customer trust variable had two dimensions: honesty and benevolence; each with five indicators. The statistical analysis method used in this research was the Wilcoxon test and partial least square test. Results: Both dimensions were dominant in measuring the customer trust variables with loading factor values which relatively similar to 0.831 for benevolence and 0.831 for honesty. Hospital staff as a dominant indicator of benevolence, given the loading factor value at 0.819 and R2 value at 0.480; hospital staff with integrity as a dominant indicator for honesty, given the loading factor value at 0.825 and R2 value at 0.472. Conclusion: Women’s customer trust of inpatient services in hospitals at West Java is in good condition. Honesty and benevolence dimension also has the same value in measuring women’s customer trust. The most dominant indicator in measuring the dimension of benevolence is hospital staff and hospital staff with integrity for the honesty dimension.Keywords: Customer trust, women, inpatient service, hospital.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47167825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of lemon on saliva and Staphylococcus aureus","authors":"Natasya Angelyna Batubara, Yumi Lindawati","doi":"10.24198/pjd.vol31no2.17955","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.17955","url":null,"abstract":"Introduction: Lemon juice contains citric acid. It is a highly antibacterial organic acid by gargling with, will be able to decrease the spreading of Staphylococcus aureus colony in saliva. The acid stimulation of lemon juice able to increase salivary secretion. The purpose of this study was to see the effect of gargling lemon juice of 100%, 50%, 25%, and 12.5% concentrations towards the salivary flow rate, salivary pH, the number of Staphylococcus aureus colony, and also, correlation between salivary flow rate and pH, salivary flow rate and number of Staphylococcus aureus colony, and salivary pH and number of Staphylococcus aureus colony. Methods: This research conducted with a pre-and-post-test group design. Saliva was taken from 24 subjects before and after the subjects gargled with lemon juice. Measurements performed were salivary flow rate per minute, and salivary pH (using pH Hanna Instrument). The Staphylococcus aureus bacteria were placed in a Mannitol Salt Agar for 24 hours at 370C temperature, then the number of the colonies were counted (x103 CFU). Results: The Wilcoxon test results showed that lemon juice with 100%, 50%, 25%, and 12.5% concentrations significantly increased the salivary flow rate and decreased the number of Staphylococcus aureus colony (p < 0.05). The result of the paired t-test showed that lemon juice with 25% and 12.5% concentration decreased the salivary pH significantly (p<0,05). The Pearson’s correlation results showed no significant correlation between salivary flow rate and salivary pH, flow rate and the number of Staphylococcus aureus colony, and salivary pH and number of Staphylococcus aureus colony. Conclusion: Lemon juice can decrease the salivary pH and the number of Staphylococcus aureus colony, but increases the salivary flow rate. It is considered to be good enough as one of the ingredients of mouthwash.Keywords: Flow rate, lemon, pH, saliva, Staphylococcus aureus","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43288752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Rahmayani, Dewi Saputri, Chairunas Chairunas, Syahrial Syahrial, M. Maisura
{"title":"Number of tooth loss in smokers and non-smokers in Monmata Lhoong Village, Aceh Besar District, Indonesia","authors":"L. Rahmayani, Dewi Saputri, Chairunas Chairunas, Syahrial Syahrial, M. Maisura","doi":"10.24198/pjd.vol31no2.19342","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.19342","url":null,"abstract":"Introduction: Tooth loss is a condition that occurs in most of the world's population. The most common causes of tooth loss are caries and periodontal disease. Periodontal disease can usually increase or getting worse by bad habits such as smoking. Smoking accelerates the course of periodontal disease and decreases salivary secretion, whereby increased the risk of caries incidence. Some components of the cigarette’s smoke that can accelerate the course of periodontal diseases are nicotine and tar. The purpose of this study was to determine the difference in the number of tooth loss in smokers and non-smokers. Methods: This study was a cross-sectional study. The sampling method was by the tables of Isaac and Michael, involving 78 smokers and 78 non-smokers in the village of Monmata Lhoong District. Results: Analysis was performed using the Mann-Whitney test. The result showed a significant difference between tooth loss in smokers and non-smokers (p < 0.05). Conclusion: The average value of tooth loss number in smokers is higher (96.47) than the non-smokers (60.53).Keywords: Tooth loss, cigarette, periodontal disease, nicotine, smoker, non-smoker","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43548371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunoexpression of Ki-67 in determining prognosis of oral cavity squamous cell carcinoma","authors":"Silvi Kintawati, H. Pramesti","doi":"10.24198/pjd.vol31no2.22888","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.22888","url":null,"abstract":"Introduction: Several researchers have been stated that the right prognosis of oral squamous cell carcinoma (OSCC) can be achieved through assessment of tumor clinical staging and tumor histopathological grading as well. The use of biomarker in histopathological grading is obviously helpful to ensure the prognosis. Objective of this study was to determine the correlation between expression of Ki-67 and histopathological grading of OSCC. Methods: A retrospective study was conducted by assessing 20 cases of paraffin-embedded OSCC derived from the Department of Pathology Anatomy of Hasan Sadikin Hospital Bandung in 2013. The OSCC performing was stained by hematoxylin-eosin. While, antibodies Ki-67 was used in immunohistochemistry reaction to determine the relation of expression of Ki-67 with histopathologic grade of OSCC for predicting a prognosis. Results: The result show that distribution of Ki-67 expression were vary from 20% to 80% in each case. Ki-67 intensity was also distributed from weak, moderate and strong. Conclusion: There is a significant correlation between Ki-67 immunoexpression and the histopathological grading of squamous cell carcinoma (p < 0.01), thus, Ki-67 can be used to predict a prognosis.Keywords: Immunoexpression, Ki-67, prognosis, oral cavity squamous cell carcinoma","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47789754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac","authors":"N. Djustiana, V. Takarini, Z. Hasratiningsih","doi":"10.24198/pjd.vol31no2.22657","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.22657","url":null,"abstract":"Introduction: Varnish fluoride has the ability to prevent enamel demineralization. De-waxed shellac that mostly used for fruits coating, now is applied as varnish fluoride materials. This de-waxed shellac is quite potential since the product can be found from Indonesian sources. The research aims to describe the toxicity effect of varnish fluoride made of de-waxed shellac by measuring the viability of the fibroblast cells. Methods: The research method that was used in this study is in vitro experimental method, which using the tooth that had been smeared with varnish fluoride contacted with fibroblast cells REF (Rat Embryonic Fibroblast) for 4, 8, and 24 hours. The toxicity effect of varnish fluoride was then measured by cell viability. Results The results show that the average cells viability on 4 hours contacted tooth was 88%, while average cells viability on 8 hours contacted tooth was 74%, and lastly the average cells viability on 24 hours contacted tooth was 63%. Conclusion: The conclusion of this preliminary study on cells viability based on toxicity effect of varnish fluoride made from Indonesian de-waxed shellac are different based on contacted time.Keywords: Cell viability, toxicity effect, varnish fluoride, Indonesian de-waxed shellac","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47641861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rosa Sharon Suhono, Endang Wahyuningtyas, Titik Ismiyati
{"title":"Silica coating application on heat-cured acrylic resin plates towards the surface hardness and the amount of residual monomers","authors":"Rosa Sharon Suhono, Endang Wahyuningtyas, Titik Ismiyati","doi":"10.24198/pjd.vol31no2.19310","DOIUrl":"https://doi.org/10.24198/pjd.vol31no2.19310","url":null,"abstract":"Introduction: Silica has been used as a coating material on acrylic resin denture plates to reduce the attachment of C. albicans, and acrylic resin denture coating application has been shown to increase the resistance. Acrylic resin as denture plate has many advantages, including good aesthetical aspect, easy to be manipulated, and relatively low costs. Acrylic resin also has disadvantages, namely the presence of residual monomers, low abrasion resistance, and broken easily. Residual monomers have poor biocompatibility and can weaken the mechanical strength of acrylic resin dentures. The purpose of this study was to analyse the silica coating application on heat-cured acrylic resin plates towards the surface hardness and the amount of residual monomers. Methods: This study used as much as 40 rod-shaped heat-cured acrylic resin specimens measured 13 x 13 x 2 mm. Specimens were divided into 2 groups (n = 20), namely the control group and the acrylic resin plate group applied with silica coating. Specimens in the treatment group applied with silica coating material using the dip-coating method. The surface hardness was tested using the Vickers hardness tester, and the amount of residual monomers was tested using gas chromatography. All data were analysed using an independent t-test. Results: Surface hardness in the group applied with silica coating was significantly higher than the control group (p < 0.05), while the remaining monomers of acrylic resin plate specimens in the group applied with silica coating were significantly lower than the control group (p < 0.05). Conclusion: Silica coating increases the surface hardness and decreases the amount of residual monomer of heat-cured acrylic resin.Keywords: Silica coating, surface hardness, residual monomer, acrylic resin","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42406554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}