Padjadjaran Journal of Dentistry最新文献

{"title":"Antibacterial and antibiofilm activity of mint leaves (Mentha piperita L) extracts against Streptococcus mutans UA159: a laboratory experiment","authors":"J. Jeffrey, Frita Ferlita Shafri Djohan, Badi Soerachman, Azmi Nur Azizah Muhtar, Adriansyah Altaf Atthoriq","doi":"10.24198/pjd.vol36no1.52744","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.52744","url":null,"abstract":"Introduction: Oral health is a major concern in healthcare worldwide, with dental caries being a prevalent issue among children and adults. Streptococcus mutans is a primary bacterium implicated in the development of dental caries due to its acidogenic nature. Mint leaf (Mentha piperita L.) is a unique herbal plant that has antibacterial and antibiofilm properties against S. mutans and minimal side effects. The purpose of this study was to analyze the antibacterial effectiveness of mint leaf against the growth of S. mutans UA159 and its antibiofilm effects. Methods: This study was conducted experimentally with the posttest-only control group design, using the broth microdilution method in 6 test groups, namely mint leaf extract with a concentration of 3.125, 6.25, 12.5, 25, 50, and 100% and also two control groups, namely the negative control using aquadest and the positive control using 0.2% chlorhexidine. Biofilm growth is determined by comparing Optical Density (OD) values and then calculating the percentage of eradication of S. mutans biofilm formation. Results: The results indicated that the mint leaf extract exhibited antibacterial effects against S. mutans, with the largest inhibition zone diameter observed at a 100% concentration. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC)90 values using the broth microdilution method in this study were 12.5% and 100% concentrations, respectively. Mint leaf extract has been shown to significantly inhibit the growth of S. mutans (p<0.05). The results of the inhibition test on biofilm formation at a concentration of 100% averaged 87.39%. Conclusion: Mint leaf extract has an antibacterial and antibiofilm effect on the growth of S. mutans. The level of inhibition of mint leaf extract on the growth of S. mutans is proportional to its concentration; the higher the concentration of the extract, the stronger the inhibitory ability.KeywordAnti-Bacterial agents, biofilms, Mentha piperita, oral health, Streptococcus mutans.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"114 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141387139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness biduri leaf extract (Calotropis gigantea) as a denture cleanser in acrylic immersion against the growth of Candida albicans: an experimental laboratory","authors":"Pudji Astuti, Achmad Gunadi, Andania Ulfa Yuga Prasetyaningrum","doi":"10.24198/pjd.vol36no1.52834","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.52834","url":null,"abstract":"ABSTRACTIntroduction: The high number of cases of tooth loss in Indonesia has led to the emergence of dentures. Dentures not appropriately kept clean can trigger cases of denture stomatitis due to Candida albicans. C. albicans is often detected on denture plates and causes denture stomatitis. Cleaning the dentures can prevent denture stomatitis, and  chemical cleaning often uses synthetic materials such as alkaline peroxide. However, alkaline peroxide can cause side effects in the form of increased porosity on the surface of the acrylic plate, so an alternative natural ingredient, namely biduri leaves (Calotropis gigantea), is needed because it has various active compounds such as alkaloids, flavonoids, phenols, saponins, terpenoids and tannins which have antifungal effects. This study aimed to determine the effective inhibitory power of biduri leaf extract as a denture cleanser in acrylic immersion against the growth of C. albicans. Methods: Biduri leaves were extracted using the maceration method with 70% ethanol solvent to produce varying concentrations of 20, 30, 40, and 50%. The sample used in this research was an acrylic plate measuring 10x10x1 mm, soaked in artificial sterile saliva for 1 hour, then soaked in each sample group for 8 hours, after that it was placed in 10 ml of Sabouraud Dextrose Broth (SDB) media, and vibrated for 30 seconds using a mixing vortex, spreading 0,1ml SDB on Saboraud Dextrose Agar (SDA) and incubated for 48 hours. The growth of C. albicans was observed and counted using the colony counting method. Result: This research showed an inhibitory power of biduri leaf extract in concentrations of 20, 30, 40, and 50% on the growth of C. albicans, with a concentration of 50%, having the most significant inhibitory power. Conclusion: Biduri leaf extract as a denture cleanser has an inhibitory power on the growth of C. albicans.KEYWORDS   Biduri leaves, Calotropis gigantea, heat cured acrylic, Candida albicans","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"114 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study","authors":"Nurdiana Dewi, Afifah Rahmadella, I. Hatta, M. L. Apriasari, Deby Kania Tri Putri","doi":"10.24198/pjd.vol36no1.51018","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.51018","url":null,"abstract":"ABSTRACTIntroduction: Caries is the most common oral disease found in society. The prevalence of caries in South Kalimantan is as high as 46.9% in 2018. S.mutans is the causative microorganism in the  initial occurrence of caries. Strategy that can be used to prevent caries is by adding nano-hydroxyapatite to the tooth paste. Nano-hydroxyapatite can be obtained from Snakehead (Channa striata) fish bone.  Snakehead is a kind of fish that is abundant in Banjarmasin.  This study aimed to analyze antibacterial activity of the nano-hydroxyapatite paste from snakehead (Channa striata) fish bone against S.mutans bacteria. Methods: The study consisted of 5 treatment groups: negative control (basic formula), positive control (casein phosphopeptide-amorphous calcium phosphate or CPP-ACP) and three treatment groups (nano-hydroxyapatite paste concentration of 10, 20 and 30%. The paste was made in the formulation of F1, F2, and F3. The antibacterial activity test by measuring MIC and MBC were performed using dilution method. Results: MIC of nano-hydroxyapatite paste was at a concentration of 10% with an average value of the difference absorbance of -0.468. MIC values in the concentration of 10%, 15%, 20% positive and negative control groups had a significant difference. MBC of nano-hydroxyapatite paste was at concentration of 15%. Concentration of 10% and the negative control group showed a significant difference, while concentration of 15% and 20% groups did not show a significant difference. Conclusion: Nano-hydroxyapatite paste from Snakehead fish bone has antibacterial activity in inhibiting and eliminating mutated S.mutans bacteria. The most effective concentration of antibacterial nano-hydroxyapatite paste to prevent caries was 10%. At this concentration, nano-hydroxyapatite can inhibit the growth of S.mutans without killing the bacteria.KEYWORDS fish bone, nano-hydroxyapatite, snakehead, S.mutans","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"1 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance analysis of DMF teeth detection using deep learning: A comparative study with clinical examination as quasi experimental study","authors":"Rizki Novita, Rizkika Putri, Maya Fitria, Maulisa Oktiana, Yasmina Elma, Handika Rahayu, Subhan Janura, Hafidh Habibie","doi":"10.24198/pjd.vol36no1.52357","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.52357","url":null,"abstract":"ABSTRACTIntroduction: Decayed, missing, and filled teeth (DMF-T) are indicators used to assess the oral health status of an individual or a population. This examination is typically performed manually by dentists or dental therapists. In previous research, researchers have developed a deep learning model as a part of artificial intelligence that can detect DMF-T. Aim of this research was to analyze the comparison of the performance of deep learning with clinical examinations in DMF-T assessment. Methods: Experienced dentists conducted clinical examinations on 50 subjects who met the inclusion criteria. Oral clinical photos of the same patients were taken from various aspects, in total 250 images, and further analyzed using a deep learning model. The results of the clinical examination and deep learning were then statistically analyzed using an unpaired t-test to determine whether there were differences between groups. Results: The unpaired t-test analysis indicated that there was no significant difference between the result of DMF-T examination by dentist and by DL (P>0.05). Unpaired t-test of this research indicated no significant difference (P = 0.161). The unpaired t-test concluded that t Stat < t Critical two-tail, then who was accepted, which stated that there was no significant difference between the results of the DMF-T examination between two groups. Conclusion: The DL model demonstrates good clinical performance in detecting DMF-T.KEYWORDS DMF-T, clinical assessment, deep learning, caries detection ","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"73 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140360247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthodontic treatment need from a bibliometric analysis of the last four decades: a bibliometric analysis","authors":"Yohana Yusra, Joko Kusnoto, I. Gunardi, Goalbertus Goalbertus, Budi Kusnoto","doi":"10.24198/pjd.vol36no1.50249","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.50249","url":null,"abstract":"ABSTRACTIntroduction: Malocclusion is a common oral disorder that strongly correlates with orthodontic treatment needs (OTN), however the complete picture of OTN remains unclear. This bibliometric study was conducted to develop a complete picture of the OTN from 1974-2022. The purpose of this study was to conduct a bibliometric analysis of scientific research pertaining to orthodontic treatment needs Methods: Type of study was bibliometric analysis. The term \"orthodontic treatment need\" was used to search for relevant articles in the Scopus database. VOSviewer, OpenRefine, and Tableau Public were used to illustrate the contributions of authors, journals, institutions, countries and the co-occurrence analysis and references analysis of the keywords. Result: There were 890 publications produced as a result of this study. Richmond emerged as the author with the most extensive publication record, having authored a remarkable 21 pieces that garnered a cumulative total of 524 citations.  The analysis reveals that the United Kingdom, Brazil, and the United States emerged as the primary contributors to literature pertaining to the assessment of orthodontic treatment necessity. The analysis of keywords revealed the occurrence of seven distinct clusters, which are: Index of Orthodontic Treatment (IOTN), orthodontic treatment, quality of life, orthodontic, malocclusion, and oral health-related quality of life. The largest cluster identified in the study was \"malocclusion,\" encompassing factors such as prevalence, the Dental Aesthetic Index (DAI), treatment necessity, and the need for orthodontic intervention. Conclusion: In general, the number of articles addressing the need for orthodontic treatment has increased, particularly in the third and fourth decades. In the fourth decade, there were more articles about the IOTN that contained keywords directly related to the index, as well as self-esteem, quality of life, and its socio-demographic and socio-economic status correlation. ","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"14 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140361049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing knowledge and attitudes about dental caries and prevention after educational intervention using a modified lecture method in adolescents","authors":"Fatharani Salsabila Az zahra, Netty Suryanti, Fidya Meditia Putri","doi":"10.24198/pjd.vol36no1.48148","DOIUrl":"https://doi.org/10.24198/pjd.vol36no1.48148","url":null,"abstract":"ABSTRACT  Introduction: The prevalence of caries in adolescents is still high, and the average visit to the dentist is when the disease is advanced. Educational interventions about dental caries and prevention need to be carried out to improve oral health status. Providing oral health education with appropriate methods will increase knowledge and encourage changes in attitudes and behaviors to prevent adolescent dental caries. This study aimed to analyze the differences in knowledge and attitudes before and after the intervention of education on dental caries and prevention among adolescents using a modified lecture method. Methods: The study design is Quasi-Experimental with one group pretest posttest research design. Sample selection used probability sampling techniques, with simple random sampling techniques. The population in this study was students of SMPN 1 Jatinangor, with the total sample of 186 respondents. Respondents were given educational intervention using a modified lecture method, namely giving a lecture, with the help of a powerpoint presentation with interesting pictures, providing a toothbrush demonstration and ending with a discussion group. Pretest and posttest assessment was using questionnaires. Pretest and posttest assessments used a questionnaire, which contained 20 knowledge questions and 15 attitude questions. Questionnaires and educational materials had been tested for validity and reliability, with Cronbach's alpha results for knowledge at 0,878 and attitude at 0,841.  The data were analyzed with the Wilcoxon test. Results: There was a significant difference between knowledge before and after the intervention of education on dental caries and prevention with a p-value of 0.001 (<0.05), and there was an increase in knowledge score, there was an average increase in knowledge score of 52%. Likewise, there were differences in attitudes before and after the intervention, with a p-value of 0.001 (<0.05) and an increase in attitude scores of 7.95%. Conclusion: The modified lecture method using power points, videos, demonstrations, group discussions and roll play on brushing teeth, which are carried out on adolescents, can increase knowledge and attitudes towards dental caries and prevention  KEYWORDS Intervention, education, knowledge and attitude, dental caries, prevention","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":"19 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140358562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The value of imbibition and syneresis for dental impression on red seaweed: a laboratory experiment","authors":"D. E. Indahyani, I. Barid, Priska Amelia Anggraeni","doi":"10.24198/pjd.vol35no3.49071","DOIUrl":"https://doi.org/10.24198/pjd.vol35no3.49071","url":null,"abstract":"ABSTRACT Introduction: Alginate is a type of elastic dental impression, which is an irreversible hydrocolloid. The main components in sodium alginate can be obtained naturally through the extraction process from seaweed (Kappaphycus alvarezii). Several physical characteristics must be fulfilled by dental impressions, such as dimensional stability related to imbibition and syneresis. ANSI/ADA specification No.18 regarding the maximum permitted change in alginate impression materials may not exceed 0.5%. This research aimed to analyze the value of imbibition and syneresis on dental impressions of red seaweed extract (K.alvarezii). Methods: The research was conducted using an experimental laboratory method with a post-test-only control group design. There were two groups, namely the control group of commercial dental impressions and the treatment group using dental impressions with the sodium alginate extracted from red seaweed (K.Alvarezii) by the acid method, and the FTIR test was performed to analyze compound sodium alginate. Imbibition and syneresis measurements were carried out using a calliper on a plaster cast model from negative mould casting results, where previously, the mould was soaked in water for imbibition and evaporated for syneresis. Results: Imbibition and syneresis tests showed that the impression material group with sodium alginate from red algae was significantly lower than those with commercial impression materials. It was concluded that the impression material with sodium alginate from red seaweed had an effect on reducing the percentage of syneresis (p≤0.05) and imbibition (p≤0.05) of the impression material. Based on the results of our research, it was found that the alginate impression material extracted from red seaweed (k.alvarezii) had an imbibition value of 0.33% (p≤0.05) and syneresis of 0.28%, (p≤0.05) lower compared to alginate impression materials. commercial alginate impression material. Conclusion: The imbibition and syneresis values of red seaweed alginate show an excessively low value compared to commercial alginates.  KEYWORDS dental impression,sodium alginate, Kappaphycus alvarezii, imbibition, syneresis","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" 48","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139623871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The severity of untreated caries in the deciduous and permanent teeth among children aged 6-12 years using PUFA and pufa index: a cross-sectional study","authors":"A. A. Suwargiani, Dudi Aripin, S. Susilawati, Netty Suryanti, Fidya Meditia Putri, Yolanda Yolanda, Ardena Hamdani Maulidia, Zamros Yuzadi Mohd Yusof","doi":"10.24198/pjd.vol35no3.51048","DOIUrl":"https://doi.org/10.24198/pjd.vol35no3.51048","url":null,"abstract":"ABSTRACT Introduction: The PUFA Index and pufa are used to measure the severity of dental and oral conditionwhich are not commonly used due to its novelty, and this index can be used to measure untreated dental caries7 and caries activity. The aims of study are to assess the severity of untreated caries in children’s deciduous and permanent teeth by using the PUFA and pufa index. Methods: An observational study using a cross-sectional study design was conducted among children who lived in Puteran village in Pager Ageung district, Tasikmalaya Regent, West Java, Indonesia. Purposive sampling was used, and the children who met the inclusion and exclusion criteria were invited to participate in the study. Severity of caries was assessed using the pufa and PUFA index for deciduous and permanent teeth, respectively. Severity of caries data were analysed using formulas for pufa and PUFA index. Results: A total of 206 children participated in the study. The PUFA assessment showed the severity of caries was high in the deciduous dentition with pufa index of 3.5 with SD 1,9-3,6 However, the PUFA assessment showed a relatively lower severity of caries in permanent teeth, with index PUFA of 0.5 with SD 1,9-3,6. The severity of caries was higher in the maxillary than the mandibular teeth in deciduous teeth. On the other hand, the severity of caries was higher in the mandibular than maxillary teeth in the permanent teeth. Conclusions: Each child aged 6 and 12 years in Puteran village had severe untreated caries in one permanent tooth and severe untreated caries in 3 to 4 of deciduous teeth. The findings of the study will be used to develop caries prevention and oral health promotion programs.KEYWORDS Caries, Severity, Deciduous, Permanent, teeth, PUFA, PUFA index, children","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139625713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing of oral health education knowledge after intervention psychoeducation methods in adolescents","authors":"G. Yubiliana, Salsabila Putri Kusumah, A. Abdurrochman","doi":"10.24198/pjd.vol35no2.48049","DOIUrl":"https://doi.org/10.24198/pjd.vol35no2.48049","url":null,"abstract":"ABSTRACT Introduction: The technique of imparting knowledge and understanding known as \"psychoeducation\" combines particular knowledge with media to communicate knowledge, is professionally provided, and integrates educational and psychotherapy treatments. Given the close connection between oral health and general health, oral health should not be neglected during the pandemic. Through the use of psychoeducational techniques, dental health issues can be prevented and overcome. The objective of this study is to analyze the increase in oral health education knowledge before and after intervention using psychoeducation methods in adolescents. Methods: This research was conducted using the one group pretest-posttest design. A study sample was obtained using the Slovin formula after a population was sampled using purposive sampling. The following requirements must be met in order for students to be included in the sample: they must be concerned about their oral health, be open to participating in psychoeducation webinars, and agree to participate as research subjects. There were 86 responses in the sample. By providing a questionnaire before (pretest) and after (posttest) the intervention in oral health education using psychoeducation method, the increase in knowledge can be measured. Results: The results of the Wilcoxon signed rank test with an alpha significance of 0.05 and Asymp. Sig (two-tailed) p = 0.001 (p < 0.05), or the knowledge factors of research respondents. This revealed significant improvements in respondents' knowledge between the pre- and post-intervention periods. Conclusion: The intervention of psychoeducational methods regarding oral health education could increase knowledge in adolescents. Psychoeducational methods can help adolescents learn more about oral health.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48077235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE EFFECT OF APPLICATION BOVINE AMNIOTIC MEMBRANE ON OSTEOBLASTS, OSTEOCYTES, AND COLLAGEN","authors":"Artdhea Regita Wibowo, Octarina Octarina, E. Munadziroh, E. Handharyani","doi":"10.24198/pjd.vol35no2.46522","DOIUrl":"https://doi.org/10.24198/pjd.vol35no2.46522","url":null,"abstract":"Introduction: Tooth extraction can result in loss of alveolar bone. The application of biomaterials Bovine Amniotic Membrane (BAM) that containing growth factors and collagen is expected to play a role in socket preservation. This study aimed to observe the effect of BAM application on the growth of osteoblasts, osteocytes, and collagen in histological preparate of the alveolar bone socket of Sprague Dawley rats (SD). Methods: 16 male SD rats were used in this group. SD rats were extracted on the mandible central incisivus. Afterward, it was divided into two groups, specifically the control group (without treatment) and the treatment group (BAM application). After extraction, the treatment group was applied with BAM with a size of 1.5 mm x 10 mm in socket alveolar bone. On the 14th and 28th day, the rats were euthanized and the alveolar bone socket of mandibular tissue was taken and stained with Hematoxylin Eosin (HE) and Masson Trichrome (MT). Results: The results of all groups showed that the number of osteoblasts and collagen thickness was higher on day 14th compared to day 28th, while osteocytes continued to increase from day 14th to 28th. The thickness of collagen, osteoblasts, and osteocytes in groups with BAM application was higher than without application. Statistical results showed that there were significant differences between groups (p<0.05). Conclusion: The application of BAM can act as material preservation socket. This biomaterial can accelerate recovery by increasing collagen thickness, osteoblasts, and osteocytes.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48457158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}