Effectiveness biduri leaf extract (Calotropis gigantea) as a denture cleanser in acrylic immersion against the growth of Candida albicans: an experimental laboratory

Padjadjaran Journal of Dentistry Pub Date : 2024-03-31 DOI:10.24198/pjd.vol36no1.52834

Pudji Astuti, Achmad Gunadi, Andania Ulfa Yuga Prasetyaningrum

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Abstract

ABSTRACTIntroduction: The high number of cases of tooth loss in Indonesia has led to the emergence of dentures. Dentures not appropriately kept clean can trigger cases of denture stomatitis due to Candida albicans. C. albicans is often detected on denture plates and causes denture stomatitis. Cleaning the dentures can prevent denture stomatitis, and chemical cleaning often uses synthetic materials such as alkaline peroxide. However, alkaline peroxide can cause side effects in the form of increased porosity on the surface of the acrylic plate, so an alternative natural ingredient, namely biduri leaves (Calotropis gigantea), is needed because it has various active compounds such as alkaloids, flavonoids, phenols, saponins, terpenoids and tannins which have antifungal effects. This study aimed to determine the effective inhibitory power of biduri leaf extract as a denture cleanser in acrylic immersion against the growth of C. albicans. Methods: Biduri leaves were extracted using the maceration method with 70% ethanol solvent to produce varying concentrations of 20, 30, 40, and 50%. The sample used in this research was an acrylic plate measuring 10x10x1 mm, soaked in artificial sterile saliva for 1 hour, then soaked in each sample group for 8 hours, after that it was placed in 10 ml of Sabouraud Dextrose Broth (SDB) media, and vibrated for 30 seconds using a mixing vortex, spreading 0,1ml SDB on Saboraud Dextrose Agar (SDA) and incubated for 48 hours. The growth of C. albicans was observed and counted using the colony counting method. Result: This research showed an inhibitory power of biduri leaf extract in concentrations of 20, 30, 40, and 50% on the growth of C. albicans, with a concentration of 50%, having the most significant inhibitory power. Conclusion: Biduri leaf extract as a denture cleanser has an inhibitory power on the growth of C. albicans.KEYWORDS Biduri leaves, Calotropis gigantea, heat cured acrylic, Candida albicans

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在丙烯酸浸泡法中作为义齿清洁剂防止白色念珠菌生长的效果：实验室实验

摘要引言：在印度尼西亚，牙齿脱落的病例很多，因此出现了假牙。假牙如果没有得到适当的清洁，就会引发白色念珠菌引起的假牙口腔炎。白色念珠菌经常在假牙板上被发现并导致假牙口腔炎。清洁假牙可以预防义齿口腔炎，化学清洁通常使用合成材料，如碱性过氧化物。然而，碱性过氧化物会导致丙烯酸树脂板表面孔隙率增加的副作用，因此需要一种替代的天然成分，即碧萝叶（Calotropis gigantea），因为它含有多种活性化合物，如生物碱、黄酮类、酚类、皂苷、萜类和单宁酸，具有抗真菌作用。本研究的目的是确定标本树叶提取物作为丙烯酸浸泡义齿清洁剂对白念球菌生长的有效抑制能力。研究方法用 70% 的乙醇溶剂浸泡法提取标本树叶，以产生 20%、30%、40% 和 50% 的不同浓度。研究中使用的样本是一个尺寸为 10x10x1 毫米的丙烯酸平板，先在人工无菌唾液中浸泡 1 小时，然后在各组样本中浸泡 8 小时，之后将其放入 10 毫升沙保鲁葡萄糖肉汤（SDB）培养基中，使用混合涡旋振动 30 秒，将 0.1 毫升 SDB 撒在沙保鲁葡萄糖琼脂（SDA）上，培养 48 小时。使用菌落计数法观察白僵菌的生长情况并进行计数。结果：研究表明，浓度分别为 20、30、40 和 50%的标本树叶提取物对白癣菌的生长具有抑制作用，其中浓度为 50%的标本树叶提取物对白癣菌的抑制作用最为显著。结论标本树叶提取物作为义齿清洁剂对白念珠菌的生长有抑制作用。关键词：标本树叶，金盏花，热固化丙烯酸树脂，白念珠菌

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来源期刊

Padjadjaran Journal of Dentistry

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审稿时长

3 weeks