Antibacterial activity of nano-hydroxyapatite paste of snakehead fish bone against S. mutans: an in vitro study

Padjadjaran Journal of Dentistry Pub Date : 2024-03-31 DOI:10.24198/pjd.vol36no1.51018

Nurdiana Dewi, Afifah Rahmadella, I. Hatta, M. L. Apriasari, Deby Kania Tri Putri

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Abstract

ABSTRACTIntroduction: Caries is the most common oral disease found in society. The prevalence of caries in South Kalimantan is as high as 46.9% in 2018. S.mutans is the causative microorganism in the initial occurrence of caries. Strategy that can be used to prevent caries is by adding nano-hydroxyapatite to the tooth paste. Nano-hydroxyapatite can be obtained from Snakehead (Channa striata) fish bone. Snakehead is a kind of fish that is abundant in Banjarmasin. This study aimed to analyze antibacterial activity of the nano-hydroxyapatite paste from snakehead (Channa striata) fish bone against S.mutans bacteria. Methods: The study consisted of 5 treatment groups: negative control (basic formula), positive control (casein phosphopeptide-amorphous calcium phosphate or CPP-ACP) and three treatment groups (nano-hydroxyapatite paste concentration of 10, 20 and 30%. The paste was made in the formulation of F1, F2, and F3. The antibacterial activity test by measuring MIC and MBC were performed using dilution method. Results: MIC of nano-hydroxyapatite paste was at a concentration of 10% with an average value of the difference absorbance of -0.468. MIC values in the concentration of 10%, 15%, 20% positive and negative control groups had a significant difference. MBC of nano-hydroxyapatite paste was at concentration of 15%. Concentration of 10% and the negative control group showed a significant difference, while concentration of 15% and 20% groups did not show a significant difference. Conclusion: Nano-hydroxyapatite paste from Snakehead fish bone has antibacterial activity in inhibiting and eliminating mutated S.mutans bacteria. The most effective concentration of antibacterial nano-hydroxyapatite paste to prevent caries was 10%. At this concentration, nano-hydroxyapatite can inhibit the growth of S.mutans without killing the bacteria.KEYWORDS fish bone, nano-hydroxyapatite, snakehead, S.mutans

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乌鳢鱼骨纳米羟基磷灰石膏对突变酵母菌的抗菌活性：体外研究

引言龋病是社会上最常见的口腔疾病。2018年，南加里曼丹的龋病发病率高达46.9%。S.mutans是最初发生龋齿的致病微生物。预防龋齿的策略是在牙膏中添加纳米羟基磷灰石。纳米羟基磷灰石可从蛇头鱼（Channa striata）的鱼骨中获得。蛇头鱼是一种盛产于 Banjarmasin 的鱼类。本研究旨在分析从乌鳢（Channa striata）鱼骨中提取的纳米羟基磷灰石糊剂对突变菌的抗菌活性。研究方法研究包括 5 个处理组：阴性对照组（基本配方）、阳性对照组（酪蛋白磷酸肽-无定形磷酸钙或 CPP-ACP）和 3 个处理组（纳米羟基磷灰石糊剂浓度分别为 10%、20% 和 30%）。糊剂的配方分别为 F1、F2 和 F3。抗菌活性测试采用稀释法测定 MIC 和 MBC。结果纳米羟基磷灰石糊剂的 MIC 值为 10%，吸光度差的平均值为-0.468。浓度为 10%、15%、20% 的阳性对照组和阴性对照组的 MIC 值差异显著。纳米羟基磷灰石糊剂的 MBC 值为 15%。浓度为 10%的组与阴性对照组差异显著，而浓度为 15%和 20%的组差异不显著。结论从蛇头鱼骨中提取的纳米羟基磷灰石糊剂具有抗菌活性，可抑制和消除变异的 S.mutans 细菌。抗菌纳米羟磷灰石糊剂预防龋齿的最有效浓度为 10%。在此浓度下，纳米羟基磷灰石可抑制变异杆菌的生长，但不会杀死细菌。

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Padjadjaran Journal of Dentistry

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3 weeks