Padjadjaran Journal of Dentistry最新文献_第7页

The effect of Indian jujube leaves extract in inhibiting the growth of Porphyromonas gingivalis 印度枣叶提取物对牙龈卟啉单胞菌生长的抑制作用

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.33007

G. Pratiwi, H. Mandalas, V. Sugiaman

{"title":"The effect of Indian jujube leaves extract in inhibiting the growth of Porphyromonas gingivalis","authors":"G. Pratiwi, H. Mandalas, V. Sugiaman","doi":"10.24198/pjd.vol34no1.33007","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.33007","url":null,"abstract":"Introduction: Periodontitis is an inflammatory disease that destroys tooth-supporting tissues and is associated with increased risk factors for systemic diseases. The main pathogen of periodontitis is the bacteria P. gingivalis, a Gram-negative, anaerobic, pleomorphic, coccobacillus, non-motile, and saccharolytic. The leaves of Indian jujube (Ziziphus mauritiana Lam.) have the main bioactive compounds such as saponins, tannins, and flavonoids which have antimicrobial activities against pathogenic microorganisms. This study aims to analyse various concentrations of the ethanol extract of Indian jujube leaves against P. gingivalis. Methods: The method used in this study was the disc diffusion test based on the Clinical and Laboratory Standard Institute. P. gingivalis preparation and the fresh leaves of Indian jujube collected from one of the plantations in Probolinggo, East Java. In this study, tests used various concentrations of Indian jujube leaves extract, namely 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100%. Data antibacterial activity was classified according to David and Stout’s inhibition zone classification. Results: Indian jujube leaves extract with a concentration between 10-30% had weak antibacterial activity, 40-60% had moderate antibacterial activity, and 70-100% had strong antibacterial activity. The largest inhibitory zone diameter against P. gingivalis was found at a concentration of 100%. Conclusion: Indian jujube leaves extract starting from a concentration of 70% can inhibit the growth of P. gingivalis with strong antibacterial activity.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42480702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison between carbonated and fruit-based soft drinks effect on calcium release from enamel surface of extracted permanent teeth 碳酸饮料与水果类软饮料对拔牙牙釉质表面钙释放的影响

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.32239

Darmayanti Siregar, G. Nazriyanti, Qanita Fadhillah

{"title":"Comparison between carbonated and fruit-based soft drinks effect on calcium release from enamel surface of extracted permanent teeth","authors":"Darmayanti Siregar, G. Nazriyanti, Qanita Fadhillah","doi":"10.24198/pjd.vol34no1.32239","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.32239","url":null,"abstract":"Introduction: People widely consume soft drinks due to their easy accessibility and delightful taste, without realising the impact on the tooth surface. Enamel dissolves easily due to acidic products that contact acid, a chemical demineralisation process (dental erosion). This study aimed to analyse the comparison between carbonated and fruit-based soft drinks effect on calcium release from enamel surface of extracted permanent teeth. Methods: In-vitro study with a time-series design was conducted on the population of extracted premolars taken from a private dental clinic and orthodontics clinic of the Faculty of Dentistry Universitas Sumatera Utara, Medan. The sample was obtained using the pairwise difference hypothesis test formula. The samples were 24 pieces divided into two treatment groups by immersing in carbonated drinks and fruit-based soft drinks for 5 to 60 minutes. The statistical test used was the independent t-test and generalised linear model-repeated measures (GLM-RM). Results: The calcium level after immersion in the carbonated drink at the fifth minute was 0.476±0.397 mg/L, then increased significantly at the sixtieth minute to 3.058±0.811 mg/L (p=0.001). In the fruit-based soft drinks immersion group, the dissolved calcium at the fifth minute was 0.671±0.208 mg/L, then increased significantly to 2.258±1.351 mg/L (p-value=0.028). Neither carbonated drinks nor fruit-based soft drinks showed a significant effect on the levels of dissolved calcium (p-value=0.135). Conclusion: In the fifth minutes of immersion, fruit-based soft drinks caused higher calcium release level compared to carbonated soft drinks. In contrast, in the sixtieth-minutes of immersion, the calcium release is found to be higher in the carbonated soft drinks group.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48309371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of brittleness and flowability between Cipetir Gutta-Percha and commercial Gutta-Percha Cipetir Gutta Percha和商用Gutta Perha的脆性和流动性比较

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.38869

Kosterman Usri, Yanwar Faza, A. Sanjaya, Nona Viona, N. Djustiana, E. Karlina, R. Febrida

{"title":"Comparison of brittleness and flowability between Cipetir Gutta-Percha and commercial Gutta-Percha","authors":"Kosterman Usri, Yanwar Faza, A. Sanjaya, Nona Viona, N. Djustiana, E. Karlina, R. Febrida","doi":"10.24198/pjd.vol34no1.38869","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.38869","url":null,"abstract":"Introduction: Gutta-Percha (GP) is a standard endodontic filling material found in pure form in the Cipetir area, Indonesia. However, a study comparing physical properties (brittleness and flowability) between pure GP and commercially used GP has not been found. Therefore, this study aims to test the brittleness and flowability of Cipetir GP compared to commercial GP. Methods: This study was quasi-experimental. Forty samples were prepared for each material and test, according to ANSI/ADA specification-GP cones-no 78 in 2006. The first step began by making a sample of Cipetir GP using moulds from a cuvette, commercial GP (Inline #80), and gypsum stone. The second step was to test the brittleness (Crease Recovery Tester) and flowability (according to ADA No. 78 of 2000) of Cipetir GP and commercial GP. Results: The brittleness test of Cipetir GP showed unbroken samples, and the commercial GP showed four broken samples. The Fisher's Exact test showed a p-value of 0.087, which means there was no significant difference in brittleness between Cipetir GP and commercial GP. At the same time, the average value of flowability of Cipetir GP and commercial GP were 6.46 mm and 0.19 mm, respectively. The unpaired t-test showed a p-value<0.05, which means there was a significant difference in the flowability between Cipetir GP and commercial GP. Conclusions: There is a brittleness similarity between Cipetir GP and commercial GP, while the flowability value of Cipetir GP is higher than commercial GP. Those initial findings showed that the Cipetir GP might become an excellent candidate to be an alternative endodontic filling.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47526750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The controlled release profile of risedronate emulgel to inhibit relapse movement in orthodontic treatment 利塞膦酸乳剂抑制正畸治疗中复发运动的控释特征

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.32628

T. R. Utari, M. F. Kurniawan, Shylvia Muchsin Andewa

{"title":"The controlled release profile of risedronate emulgel to inhibit relapse movement in orthodontic treatment","authors":"T. R. Utari, M. F. Kurniawan, Shylvia Muchsin Andewa","doi":"10.24198/pjd.vol34no1.32628","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.32628","url":null,"abstract":"Introduction: Relapse is one of the undesirable effects of orthodontic treatment. Prevention of relapse has been carried out with the use of retainer devices. Several studies also have been carried out to prevent relapse with pharmacological agents such as bisphosphonates. One of the strongest bisphosphonates is risedronate. Systemic use of bisphosphonates can cause bisphosphonate-related necrosis of the jaw (BRONJ). Systemic effects can be minimised by topical preparations locally, where the virgin coconut oil (VCO) emulgel is one of the topical preparations which controls the release of drugs. This study aims to analyse the release profile of risedronate emulgel as a material to inhibit relapse movement. Methods: This research was conducted in an experimental laboratory. Group 1 was emulgel without bisphosphonate risedronate with virgin coconut oil (VCO), Group 2 was VCO emulgel with bisphosphonates risedronate, and Group 3 was a pure bisphosphonate risedronate solution. Each group weighing 100 mg was placed in 10 ml PBS, and the release test was conducted with UV/VIS Spectrophotometer wavelength λ 262 nm at intervals of 1, 2, 4, 8, 24, 48, and 96 hours with three replications at each group. Results: Grup 2 yielded a controlled drug release of risedronate until 96 hours, while a pure solution of risedronate resulted in an uncontrolled drug release of risedronate, which was released entirely in 4 hours. Conclusion: Risedronate emulgel with VCO had a controlled drug release compared to pure bisphosphonate solution to potentially be applied topically to inhibit relapse movement.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47649111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of severity level, stress, and bad habit among dental students with exfoliative cheilitis 牙科学生剥脱性唇炎的严重程度、压力及不良习惯的评估

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.28781

Sangary Sri Sivabalasundram, E. Herawati, Winny Yohana

{"title":"Evaluation of severity level, stress, and bad habit among dental students with exfoliative cheilitis","authors":"Sangary Sri Sivabalasundram, E. Herawati, Winny Yohana","doi":"10.24198/pjd.vol34no1.28781","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.28781","url":null,"abstract":"Introduction: Exfoliative cheilitis (EC) is one of the most common cheilitis found in the community. This lesion can cause difficulty in mouth opening, speech difficulties, and reduced aesthetical value of the lips. EC is a chronic inflammation characterised by desquamation, erythema, and crusting on the upper or lower lip. This study evaluated severity level, stress, and bad habit among dental students with exfoliative cheilitis. Methods: The method used in this research was descriptive with a total sampling method on the populations of dental students batch of 2016 to 2018 of the Faculty of Dentistry Universitas Padjadjaran, which obtained 83 dental students with EC. The lips were then examined, and data was recorded. EC was classified into three stages in clinical manifestation: mild, moderate, and severe exfoliative cheilitis. The data were presented with a simple frequency distribution table. Result: 30.1% of the research subjects had mild EC, 53% had moderate EC, and 16.9% had severe EC. The predisposing factors of EC in this research were stress and bad habits, where the percentage contribution were 53% and 16.9%, respectively. Students were also found with both stress and bad habits with a percentage contribution of 30.1%. Conclusions: Moderate exfoliative cheilitis is most commonly found with desquamation and erythema clinical features. The predisposing factor of exfoliative cheilitis is stress which has the highest percentage among dental students.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45537439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The relationship of the formation of dental fear between mothers and their 7 to 9 year-old children 母亲与7 ~ 9岁儿童牙齿恐惧形成的关系

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.38870

J. Cheema, Meirina Gartika, Y. Herdiyati

{"title":"The relationship of the formation of dental fear between mothers and their 7 to 9 year-old children","authors":"J. Cheema, Meirina Gartika, Y. Herdiyati","doi":"10.24198/pjd.vol34no1.38870","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.38870","url":null,"abstract":"Introduction: Dental fear is a common occurrence that responds to the stress induced by various dental procedures. Its intensity varies from nervousness and anxiety to dental phobia, and it is considered the main barrier to successful completion of treatment. This research aims to analyse the relationship of the formation of dental fear between mother and their 7 to 9-year-old children. Methods: The research design was analytical studies with a cross-sectional survey technique, with samples chosen by the simple random sampling method. Thirty-five mothers and children aged 7-9 years who attended Neglasari 2 and Neglasari 5 Public Elementary School, Sadang Serang, Bandung, were the respondents. This study used a questionnaire called the Dental Subscale of the Children's Fear Survey Schedule (CFSS-DS), tested and proven to be a reliable and valid psychometric instrument for dental fear evaluation in children in Bahasa Indonesia. This study was analysed using Spearman's rank correlation coefficient. Results: The mean score of the mothers was 24.314, and the mean score of the children was 23.571. The correlation value was 0.765. The figure scales in the 'strong' criteria. Conclusion: There is a strong relationship between the formation of dental fear in mothers and their 7-9 years-old children.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41738165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits 富血小板纤维蛋白对牙周炎兔牙龈结缔组织胶原密度的影响

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.31891

A. Nasution, Wenny Dewanti

{"title":"Efficacy of platelet-rich fibrin application for improving collagen density of gingival connective tissue in periodontitis-induced rabbits","authors":"A. Nasution, Wenny Dewanti","doi":"10.24198/pjd.vol34no1.31891","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.31891","url":null,"abstract":"Introduction: Due to the high prevalence of periodontal diseases that increase the possibility of tooth loss, platelet-rich fibrin (PRF) as a regenerative treatment has been proposed. PRF is a potential second-generation platelet concentrate and autologous healing biomaterial which contain growth factors that accelerate wound healing mechanisms. This study aimed to evaluate the efficacy of platelet-rich fibrin application to improve gingival connective tissue collagen density in periodontitis-induced rabbits. Methods: An experimental study with a post-test only control group design approach was conducted on 20 samples calculated using the Federer formula. Periodontitis was induced in ten rabbits with silk ligatures tied around the cervical region of central incisors teeth bilaterally for 14 days. After periodontitis occurred, phase I treatment was performed and followed by curettage. PRF was placed in the lower region as a treatment group using a split-mouth design, whereas a control group without PRF was in the upper jaw region. Subjects were euthanised after the 7th and 14th days of healing. Histopathological analysis was performed, and collagen density was quantified using ImageJ software. Statistical analysis was carried out using paired-T and two-way ANOVA tests. Results: There was a significant difference (p=0.001; p<0.05) in gingival connective tissue collagen density between treatment and control groups on the 7th and 14th days of observation. There was a significant increase in gingival connective tissue collagen density in the treatment group. Conclusion: Platelet-rich fibrin could improve gingival connective tissue collagen density in periodontitis-induced rabbits.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44860721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences in the salivary flow rate, buffer capacity, and pH, based on the length of inhalants usage in bronchial asthma patients 支气管哮喘患者唾液流速、缓冲容量和pH值的差异，基于吸入剂的使用时间

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.29617

Naomi Tio Agustine Sinaga, Rosilawati Wihardja, K. I. Sari

{"title":"Differences in the salivary flow rate, buffer capacity, and pH, based on the length of inhalants usage in bronchial asthma patients","authors":"Naomi Tio Agustine Sinaga, Rosilawati Wihardja, K. I. Sari","doi":"10.24198/pjd.vol34no1.29617","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.29617","url":null,"abstract":"Introduction: Asthma or bronchial asthma is a chronic respiratory tract inflammation process involving many cells, including their elements, and can cause severe and plentiful responses from the respiratory tract. Administration of inhalation drugs is the primary medication in treating bronchial asthma. Inhalants are given appropriately with dosage and usage period based on the severity level of bronchial asthma. This research aimed to analyse the differences in the salivary flow rate, buffer, and pH, based on the length of inhalants usage in bronchial asthma patients. Methods: The research was descriptive-comparative involving 40 bronchial asthma patients (6 male and 34 female) from the Community Lung Health Center of Bandung, taken as samples using the central theorem limit sampling method. The subjects were ordered to collect their saliva in the saliva collection cups with a draining method. Then, the salivary flow rate, buffer, and pH were examined. Data distribution was tested, resulting in normally distributed data; thus, a parametric independent t-test with a significant level of 0.05 was applied. Results: Subjects in the group of inhalant usage for more than ten years had low and very low categories of salivary flow rate, buffer, and pH. There were significant differences in the salivary flow rate, buffer, and pH of the bronchial asthma patients based on their length of inhalants usage (1-5 years; >5 years–10 years; >10 years; p<0.05). Conclusions: There are differences in the salivary flow rate, buffer, and pH based on the length of inhalants usage in bronchial asthma patients. The longer the usage period, the lower the salivary flow rate, buffer, and pH.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43199050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis (ATCC 10556) 草莓果实提取物对血链球菌的抗菌活性（ATCC 10556）

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.37043

Denny Nurdin, H. A. Dharsono, Fajar Fatriadi, Elvi Sahara, Rahmi Alma Farah Adang, Yolanda Dwiutami Gondowidjojo, D. Kurnia

{"title":"Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis (ATCC 10556)","authors":"Denny Nurdin, H. A. Dharsono, Fajar Fatriadi, Elvi Sahara, Rahmi Alma Farah Adang, Yolanda Dwiutami Gondowidjojo, D. Kurnia","doi":"10.24198/pjd.vol34no1.37043","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.37043","url":null,"abstract":"Introduction: Streptococcus sanguinis is a facultative anaerobic Gram-positive bacteria known as a pioneer that plays a role in creating the biofilm in the oral cavity. Strawberry fruit (Fragaria x ananassa) is an edible fruit widely used for the study as their active compound synergy to improve health. This study aims to analyse the antibacterial activity of strawberry fruit against S. sanguinis (ATCC 10556). Methods: An explorative study was conducted with high concentration methanol extract from strawberry fruit. Antibacterial activity was tested on the methanol extract; afterwards, the extract was fractionated and divided into three fractions: water, ethyl acetate, and hexane. Zone of inhibition was used to assess the most effective fraction among those three, then continued by testing for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Inhibitory zone of the ethyl acetate fraction from strawberry fruit 1%, 2%, 3% ,4% and 5% sequentially were 7.3 mm,10.2 mm, 12.3 mm, 16.3 mm and 16.1 mm. Ethyl acetate fraction of 4% was the most effective to create the zone of inhibition with a size of 16.3 mm compared to the others. The Minimum Inhibitory Concentration (MIC) value was 0.25%. It was obtained by diluting a 4% ethyl acetate fraction on a microplate. The Minimum Bactericidal Concentration (MBC) was 2%. Conclusion: Ethyl acetate fraction was an effective fraction from strawberry fruit and had antibacterial activity against S. sanguinis with the inhibitory zone in the concentration of 4%, MIC of 0.25%, and MBC of 2%.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47905961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment 根管治疗初期7天，氢氧化钙药物对引起慢性根尖周脓肿的细菌的MIC和MBC比较

Padjadjaran Journal of Dentistry Pub Date : 2022-04-09 DOI: 10.24198/pjd.vol34no1.28638

Chany Mony Dwiayu Putri, D. Prisinda, Y. Malinda

{"title":"The MIC and MBC of calcium hydroxide medicament against bacteria that cause chronic periapical abscess in the vulnerable initial 7-days of endodontic treatment","authors":"Chany Mony Dwiayu Putri, D. Prisinda, Y. Malinda","doi":"10.24198/pjd.vol34no1.28638","DOIUrl":"https://doi.org/10.24198/pjd.vol34no1.28638","url":null,"abstract":"Introduction: The primary aetiology of chronic periapical abscesses, including E. faecalis, S. mutans, S. sanguinis, and P. gingivalis, can be eliminated using intracanal medicaments such as calcium hydroxide. The purpose of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) value of calcium hydroxide paste in the vulnerable initial 7-day of treatment against E. faecalis ATCC 29212, S. mutans ATCC 25175, S. sanguinis ATCC 10556, and P. gingivalis ATCC 33277. Methods: An in-vitro laboratory experiment using a spectrophotometer as a microdilution method was conducted to determine bacteria's MIC and MBC values on calcium hydroxide paste. The sample used in this study was four bacteria using intracanal medicament of calcium hydroxide (Ca(OH)2), incubated for seven days at 37°C, and then bacterial growth was observed. The cell inhibition percentage was calculated using optical density measurements to determine the MIC value. The low MIC and MBC were defined as sensitive bacteria to calcium hydroxide. Results: Calcium hydroxide paste against E. faecalis (ATCC 29212) with MIC values at a concentration of 750 μg/ml and MBC values at a concentration of 96,000 μg ml; S. mutans (ATCC 25175) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 48,000 ug/ml; S. sanguinis (ATCC 10556) with MIC value at a concentration of 3,000 ug/ml and MBC value at a concentration of 6,000 ug/ml; P. gingivalis (ATCC 33277) with MIC value at a concentration of 6,000 ug/ml and MBC value at a concentration of 48,000 ug ml. Conclusions: Calcium hydroxide can inhibit bacterial growth activity. E. faecalis (ATCC 29212) and S. sanguinis (ATCC 10556) are more sensitive to calcium hydroxide paste than other bacteria, with the lowest MIC and MBC on seven days of incubation since the maximum calcium and hydroxyl ions are released.","PeriodicalId":31757,"journal":{"name":"Padjadjaran Journal of Dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48357815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2