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Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp. 芽孢杆菌抑制沙葱扭曲病的诱导抗性机制。
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2022-07-11 DOI: 10.22146/jpti.73198
Elfrida Indriani Reno Wulan, A. Wibowo, T. Joko, A. Widiastuti
{"title":"Induced Resistance Mechanism of Twisted Disease Suppression of Shallot by Bacillus spp.","authors":"Elfrida Indriani Reno Wulan, A. Wibowo, T. Joko, A. Widiastuti","doi":"10.22146/jpti.73198","DOIUrl":"https://doi.org/10.22146/jpti.73198","url":null,"abstract":"Plant Growth Promoting Rhizobacteria has been known for its ability to induce plant resistance on shallot against twisted disease. Its ability as a bioprotectant agent is estimated to be comparable to the efficacy of Trichoderma which is currently widely used as a biological control agent.. This study aims to determine the content of jasmonic acid, salicylic acid, peroxidase, and disease suppression in shallot by application of Bacillus velezensis B-27, Bacillus cereus RC76, and application with combination of both rhizobacteria. The application was carried out  with tuber dipping for 30 minutes in each treatment with a bacterial density of 108 CFU mL-1. Application using Trichoderma was used as the comparison treatment, and the control plot was not given any treatment. Pathogen inoculation was carried out simultaneously as planting using Fusarium acutatum with a spore density of 106 CFU mL-1. The jasmonic and salicylic acids content was measured using the High-Performance Liquid Chromatography method, and the peroxidase content was determined using the spectrophotometric method. Disease suppression was measured at 10-day intervals. The results showed that treatment with Bacillus cereus RC76 increased jasmonic and salicylic acid levels, while application with Bacillus velezensis B-27 showed the highest level of peroxidase. Treatments with Bacillus spp. were able to suppress twisted disease by 72.2% to 100%. This study demonstrated that application Bacillus spp. suppressed twisted disease on shallot and increased the jasmonic and salicylic acid content as induced resistance mechanism against pathogens.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49540226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Decomposition of Wood by Termites in Different Types of Land Use 白蚁对不同土地利用类型木材的分解作用
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2022-07-11 DOI: 10.22146/jpti.69322
Sri Heriza, D. Buchori, I. Harahap, N. Maryana
{"title":"Decomposition of Wood by Termites in Different Types of Land Use","authors":"Sri Heriza, D. Buchori, I. Harahap, N. Maryana","doi":"10.22146/jpti.69322","DOIUrl":"https://doi.org/10.22146/jpti.69322","url":null,"abstract":"Decomposition is an important process in nature, as it can break down organic matter into smaller particles and nutrients. Nutrients are returned to the environment and can be reused by other organisms. One important organism that plays a role in the decomposition process is termites because termites are able to decompose organic matter such as wood in all habitats. Different termite habitats can be seen in terms of different types of land use. Some types of land use, such as natural forests, secondary forests, palm oil plantations, and settlements, can be used as a test site for wood decomposition by termites. All types of land use can be found in Dharmasraya Regency, West Sumatra Province. The purpose of this study was to study the decomposition of wood by termites in several different types of land use. Four types of land use are selected from habitats with the least disturbed conditions to the extent of high habitat disturbances, namely natural forests, secondary forests, palm oil plantations, and settlements. The study was conducted in Dharmasraya Regency, West Sumatra Province. The research method uses pine wood bait (Pinus merkusii), measuring 1.9 cm x 1.9 cm x 25 cm. The wooden bait is winded for seven days, and the wood bait is weighed to gain its initial weight and measured moisture content. Furthermore, wood bait that has been installed is observed and re-weighed to gain final weight and measured water content. The results showed that deforestation in Dharmasraya, West Sumatra had an effect on reducing wood decomposition by termites. Five (5) termite species were found in wood bait, including Pericapritermes sp.1, Microtermes sp.1, Schedorhinotermes sp.1, Macrotermes gilvus, and Coptotermes curvignathus.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46624733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Parasitization Levels of Spodoptera frugiperda Eggs (Smith) (Lepidoptera: Noctuidae) in Three Different Corn Ecosystems in East Java 东爪哇三种不同玉米生态系统草地贪夜蛾卵(Smith)(鳞翅目:夜蛾科)的寄生水平
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2022-06-29 DOI: 10.22146/jpti.71598
Rizki Dwi Wahyuningsih, T. Harjaka, Suputa Suputa, Y. Trisyono
{"title":"Parasitization Levels of Spodoptera frugiperda Eggs (Smith) (Lepidoptera: Noctuidae) in Three Different Corn Ecosystems in East Java","authors":"Rizki Dwi Wahyuningsih, T. Harjaka, Suputa Suputa, Y. Trisyono","doi":"10.22146/jpti.71598","DOIUrl":"https://doi.org/10.22146/jpti.71598","url":null,"abstract":"Spodoptera frugiperda  (Lepidoptera: Noctuidae) has successfully invaded and spread to almost all provinces of Indonesia which may cause significant impacts on corn production. Local natural enemies could play an important role in managing this invasive insect, and more diverse ecosystems would benefit natural enemies. This study aimed to assess parasitization rates of S. frugiperda eggs by local egg parasitoids in three different corn ecosystems (agroforestry, rice field, and rainfed field) in East Java. Sentinel egg masses were used for this study by exposing eight-hour-old egg masses collected from the laboratory mass-rearing and left for 24 hours in corn plantations aged 7, 14, and 28 days after planting. Telenomus sp. was more abundant in the three ecosystems compared to Trichogramma sp. The egg mass parasitization varied from 15.6 to 52.5%. The number of egg masses parasitized was consistently higher in agroforestry, followed by rice fields and rainfed fields in all three different sampling times. Interestingly parasitization rates on egg masses were not different, and they ranged from 43.7 to 81.6%. These findings provide evidence on the importance of local egg parasitoids for managing S. frugiperda and some insights related to plant diversity to improving the services by these parasitoids. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42362708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Biology and Demography of Helopeltis bradyi Waterhouse (Hemiptera: Miridae) Reared on Cucumbers 黄瓜上饲养的布氏斑蝶的生物学和种群学
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2022-06-28 DOI: 10.22146/jpti.71761
Rich Gemilang Simanjuntak, T. Harjaka, A. Wijonarko
{"title":"Biology and Demography of Helopeltis bradyi Waterhouse (Hemiptera: Miridae) Reared on Cucumbers","authors":"Rich Gemilang Simanjuntak, T. Harjaka, A. Wijonarko","doi":"10.22146/jpti.71761","DOIUrl":"https://doi.org/10.22146/jpti.71761","url":null,"abstract":"Helopeltis bradyi is often used as for research and is required in large numbers. The insects were obtained from cacao plants and reared with cucumber fruit in the laboratory. This study aimed to determine the biology and demography of H. bradyi reared on cucumber under laboratory conditions. The demographic parameters observed were net reproduction rate (Ro), gross reproduction rate (GRR), intrinsic growth rate (r), average generation period (T), and population doubling (DT). The results showed that the egg stage lasted for 6.33 ± 0.47 days, the developmental period of the I‒V instar nymph was 2.13 ± 0.34, 2.07 ± 0.25, 2.13 ± 0.34, 2.33 ± 0.47, and 3.20 ± 0.40 days. The lifespan of male imagoes were 32.33 ± 4.92 days, and female imagoes were 24.60 ± 6.64 days. The adult female pre-oviposition period was 2.2 ± 0.40 days and oviposition period of 22.9 ± 4.66 days. H. bradyi has a type III survival curve which indicated high mortality rates during the first instar egg and nymph population. Demographic statistics of H. bradyi on cucumbers fruit are GRR of 296 individuals per generation, Ro of 196 individuals per parent per generation, rate of r of 0.18 individuals per parent per day, T of  29.34 days, and DT of  3.85 days.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46948772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Application of Vermicompost with Different Feeding Material for Inducing Rice Plant Resistance against Brown Planthopper (Nilaparvata lugens Stål.) Attack 不同投料材料的粘虫复合物在水稻抗褐飞虱中的应用
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-31 DOI: 10.22146/jpti.60824
D. Hastuti, E. P. Ningsih, S. Ritawati, R. Khastini
{"title":"Application of Vermicompost with Different Feeding Material for Inducing Rice Plant Resistance against Brown Planthopper (Nilaparvata lugens Stål.) Attack","authors":"D. Hastuti, E. P. Ningsih, S. Ritawati, R. Khastini","doi":"10.22146/jpti.60824","DOIUrl":"https://doi.org/10.22146/jpti.60824","url":null,"abstract":"Brown planthopper (BPH) is a major pest in rice plants and has become a global pest. This pest causes hopperburn in rice plants. This research aimed to know the effect of vermicompost from different feeding materials on rice plant damage by brown planthopper attack. This research was carried out in November 2019–March 2020 at the Sistandu integrated farming system, Serang City, Banten. The experiment method used in this research was a factorial Randomized Block Design (RBD) consisting of two factors with three replications. The first factor was vermicompost (P) 5 levels, while the second factor was rice varieties (V) with two groups so that there were ten treatments. Data were analyzed with ANOVA, then followed by 5% DMRT. The results showed that the application of vermicompost with different feeding materials gave non-significant results to the growth of rice plants in the vegetative phase and fecundity of the BPH. However, it significantly affected the intensity of rice plant damage in the generative phase, the offspring population of BPH, and the BPH feeding rate. While the use of two varieties of rice does not have a distinct effect on all parameters. There is no interaction effect between two factors, both vermicompost feeding material treatment and rice plant variety, to all the parameters that have been studied.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42665799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Characterization and Pathogenicity of Rhizoctonia solani Kühn Associated with Sheath Blight in Local Rice Varieties (Oryza sativa L.) of Special Region of Yogyakarta Yogyakarta特殊地区水稻品种(Oryza sativa L.)与纹章病相关的丝核菌Kühn的特性及致病性
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-17 DOI: 10.22146/jpti.67557
Maulin Nafisa, Adiana Nayogyani, R. Kasiamdari
{"title":"Characterization and Pathogenicity of Rhizoctonia solani Kühn Associated with Sheath Blight in Local Rice Varieties (Oryza sativa L.) of Special Region of Yogyakarta","authors":"Maulin Nafisa, Adiana Nayogyani, R. Kasiamdari","doi":"10.22146/jpti.67557","DOIUrl":"https://doi.org/10.22146/jpti.67557","url":null,"abstract":"In Indonesia, rice is used as the first strategic food commodity and a priority in agricultural development. However, in practice, efforts to increase it still experience various obstacles, one of which is caused by the attack of pathogen Rhizoctonia solani. This study aimed to determine the characters of R. solani and observe its pathogenicity on some local rice varieties in the Special Region of Yogyakarta. Four isolates of R. solani were used, namely RS MS2, RS MKP2, RS UMB, and RS PN, as well as six varieties of local rice varieties, which were Menthik Susu, Cempo Merah, Cempo Hitam, Segreng, Menoreh, and Hitam Makaryo, and national rice varieties IR 64 and Ciherang. Morphological characterization was carried out based on the cultural characteristics of the colony, hyphae, and sclerotia. A pathogenicity test was carried out using the Detached Leaf Inoculation method. The result showed that R. solani characters vary from whitish-brown, yellowish-brown, and light brown colonies; moderate and fast growth rates; abundant-aerial, moderate-aerial, and slight-aerial growth patterns; 90° hyphae branching, narrowing of hyphae at the branching point, and presence of sclerotia. The pathogenicity test gave the results of all isolates having varying pathogenicity levels on each rice variety. Menthik Susu, Cempo Hitam, and Hitam Makaryo were resistant to R. solani. Cempo Merah and Segreng were considered as moderately resistant varieties, whereas Menoreh was categorized as moderately susceptible. Ciherang and IR64 were susceptible varieties to R. solani.","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42396487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Arthropod and Earthworm Populations on Irrigated Rice Farming Land after Paraquat Herbicide Application 施用百草枯除草剂后灌溉稻田节肢动物和蚯蚓种群
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-13 DOI: 10.22146/jpti.54363
E. Martono, Ruwinda Wahyu Wijayanti, Kusnanik Kusnanik, D. V. Torani
{"title":"Arthropod and Earthworm Populations on Irrigated Rice Farming Land after Paraquat Herbicide Application","authors":"E. Martono, Ruwinda Wahyu Wijayanti, Kusnanik Kusnanik, D. V. Torani","doi":"10.22146/jpti.54363","DOIUrl":"https://doi.org/10.22146/jpti.54363","url":null,"abstract":"Paraquat dichloride is a herbicide compound commonly used to control grasses and broadleaf weeds. Its use is presently under scrutiny as there is a report that this herbicide is very harmful and hazardous to the environment, especially to wild fauna surrounding the farming sites. An irrigated rice farming locale experiment was conducted to observe the effect of paraquat treatment on rice plant biotic environment, particularly its arthropods and earthworm population, two of the most prominent fauna easily affected by paraquat. The study was carried out in Seyegan District, Sleman Regency, Special Region of Yogyakarta Province, on irrigated rice land from October 2018 to February 2019. Four treatments of paraquat, i.e. control (0 kg/ha paraquat), lower dose (0.8 kg/ha), medium dose (1.6 kg/ha), and higher dose (3.2 kg/ha) were arranged. Carbendazim 5 kg/ha was also applied as comparing pesticides, especially for paraquat’s effect on the earthworm. The treatments were replicated five times in a plot of 5 x 5 m2. The number of arthropods and earthworms was observed in about two weeks intervals or more. The observation was done for the number of arthropods and their families, while for earthworms, only for their numbers. The arthropods population data were subjected to diversity indices and statistical analysis, while the earthworms data were statistically analyzed. The result showed that the arthropod numbers in treated plots were statistically significantly different, but biologically their number was still abundant. Their diversity did not differ from the control treatment at all. The arthropod numbers were 2007, 1483, 1095, 1746, and 1204 in control, lower, medium, higher, and carbendazim plots. The earthworms' numbers were still plenty; those in the higher dosage did not differ significantly from those in control. Their numbers from control, lower, medium, higher dose, and carbendazim plots were 811, 658, 567, 882, and 445 individual earthworms. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49095360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Susceptibility of Two Varieties of Guava (Psidium guajava L.) to Pest and Disease Infection in the Sub-District of Tanah Sareal, Bogor 两种番石榴品种在茂物塔那沙雷街道对病虫害的敏感性
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-10 DOI: 10.22146/jpti.49851
H. Triwidodo, E. T. Tondok, Desy Nur Avifah
{"title":"Susceptibility of Two Varieties of Guava (Psidium guajava L.) to Pest and Disease Infection in the Sub-District of Tanah Sareal, Bogor","authors":"H. Triwidodo, E. T. Tondok, Desy Nur Avifah","doi":"10.22146/jpti.49851","DOIUrl":"https://doi.org/10.22146/jpti.49851","url":null,"abstract":"This survey was done in several guava fields around Bogor and found many unhealthy plants infested by various plant pests and diseases. This study aimed to analyze the effect of various guava cultivars on pest and disease intensity on guava in the Sub-District of Tanah Sareal, Bogor. Samples were collected from 20 fields that consisted of 15 samples of Getas Merah and 5 samples of Bangkok Putih cultivars to identify plant pests and diseases. Interviews were done with field owners to obtain information on cultivating practices. Pests found based on morphological identification included plant bugs (Hemiptera: Miridae), locusts (Orthoptera: Acrididae), bagworms (Lepidoptera: Psychidae), Geometrid caterpillars (Lepidoptera: Geometridae), Pyralid caterpillars (Lepidoptera: Pyralidae), scale insects (Hemiptera: Coccidae), planthoppers (Hemiptera: Flatidae), and mealybugs (Hemiptera: Pseudococcidae). Disease and pathogens identified included red rust disease (Cephaleuros sp.), anthracnose (Colletotrichum gloeosporioides), and fruit cancer (Pestalotia sp.). The occurrence of biting-chewing type pests was greater than piercing-sucking type insects, including 33.33% and 34.99%. Red rust disease was the dominant disease found on both guava cultivars consisting of 68.88% and 63.33% of the samples. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49214117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diversity of Beneficial Insect in Corn Plantation at West Sumatra 西苏门答腊玉米种植园有益昆虫的多样性
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-09 DOI: 10.22146/jpti.61254
Martinus Abuzar, U. Khairul, H. Hamid
{"title":"Diversity of Beneficial Insect in Corn Plantation at West Sumatra","authors":"Martinus Abuzar, U. Khairul, H. Hamid","doi":"10.22146/jpti.61254","DOIUrl":"https://doi.org/10.22146/jpti.61254","url":null,"abstract":"The corn plant is one of Indonesia's agricultural products with economical and strategic value and many potential to develop, such as the main carbohydrates and protein source. There are two types of beneficial insects in corn planting: predators and parasitoids. This study aims to know the diversity of beneficial insects in corn plantations. It was conducted from May – September 2019 in four different locations across West Sumatra, namely Padang Pariaman, West Pasaman, Pesisir Selatan, Solok Regencies. Identification of beneficial insects was made in Entomology Laboratory, Faculty of Agriculture, Andalas University. This study adopted a purposive sampling method using swing net 20 times, following the transect line of corn planting. The result showed five orders, 34 families, 101 morphospecies, and 747 beneficial insects in all locations. The diversity in each place varies from high, medium, and low. In all areas, the diversity index was high, with the criteria of ≥ 2.90, which means that the condition of the community structure was very stable. From the evenness index analysis, three locations were classified as high, and the other one was moderate. These three sites were Padang Pariaman, West Pasaman, and Solok, with criteria of ≥ 0.90 and very stable distribution conditions. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48100215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Double Infections of Rehmannia mosaic virus and Potato virus Y on Tobacco Plants in Central Java and Special Region of Yogyakarta 地黄花叶病毒和马铃薯Y病毒对爪哇中部和日惹特别地区烟草的双重感染
Jurnal Perlindungan Tanaman Indonesia Pub Date : 2021-12-09 DOI: 10.22146/jpti.67468
Tika Pramudya Wardani, S. Hartono, S. Sulandari, S. Somowiyarjo
{"title":"Double Infections of Rehmannia mosaic virus and Potato virus Y on Tobacco Plants in Central Java and Special Region of Yogyakarta","authors":"Tika Pramudya Wardani, S. Hartono, S. Sulandari, S. Somowiyarjo","doi":"10.22146/jpti.67468","DOIUrl":"https://doi.org/10.22146/jpti.67468","url":null,"abstract":"Various viruses can cause mosaic disease in tobacco plants. Proper detection of the cause of the mosaic disease helps determine effective control. The purpose of this study was to detect the presence of double infection of Rehmannia mosaic virus (ReMV) with Potato virus Y (PVY) using multiplex RT-PCR in tobacco plants from Central Java and Special Region of Yogyakarta. The viral suspension was inoculated on Chenopodium amaranticolor to obtain one viral colony from one local lesion. The multiplex RT-PCR method using Tobamovirus primers (TobRT-up1 and TobRT-do2) and Potyvirus primers (MJ1 and MJ2) can detect double infection caused by ReMV with PVY in tobacco plants distributed in Central Java and Special Region of Yogyakarta. The multiplex RT-PCR product showed that tobacco samples with mosaic symptoms from Temanggung, Klaten, Bantul, and Kalasan were positive ReMV. Multiplex RT-PCR has successfully detected double infection of ReMV and PVY on tobacco samples from Klaten and Kalasan. ReMV Bantul, Kalasan, and Klaten were homolog to ReMV USA isolate and ReMV Temanggung was homolog to ReMV Japanese isolate. PVY Klaten was homolog to PVY Turkey isolate, and PVY Kalasan was homolog to PVY Iran. ","PeriodicalId":31599,"journal":{"name":"Jurnal Perlindungan Tanaman Indonesia","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43050297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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