Journal of Biological Researches最新文献_第5页

THE EXPRESSION OF TLR-2 AND NOD-2 IN GINGIVAL EPITHELIUM OF RAT AFTER PROBIOTIC Lactobacillus reuteri SUPPLEMENTATION TO INHIBIT Streptococcus mutans GROWTH 补充益生菌罗伊氏乳杆菌抑制变形链球菌生长后大鼠牙龈上皮中TLR-2和NOD-2的表达

Journal of Biological Researches Pub Date : 2016-02-28 DOI: 10.23869/BPHJBR.21.1.20156

Tuti Kusumaningsih, S. Sudarmo, Retno Indrawati, R. D. Ridwan, S. Sidarningsih

{"title":"THE EXPRESSION OF TLR-2 AND NOD-2 IN GINGIVAL EPITHELIUM OF RAT AFTER PROBIOTIC Lactobacillus reuteri SUPPLEMENTATION TO INHIBIT Streptococcus mutans GROWTH","authors":"Tuti Kusumaningsih, S. Sudarmo, Retno Indrawati, R. D. Ridwan, S. Sidarningsih","doi":"10.23869/BPHJBR.21.1.20156","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20156","url":null,"abstract":"Lactobacillus reuteri is probiotic from Gram positive bacteria which has specific molecular structure, consisted of peptidoglycan (PG) and lipotheihoic acid (LTA). These structure have potential in pattern recognition receptors (PRRs) activation, such as TLR-2 and NOD-2 that are the up stream of beta defensin-2 (BD-2) signaling cascade. BD-2 is antimocrobial peptides naturally produced in mouth cavity that can against Streptococcus mutans effectively. This study was aimed to prove that probiotic L. reuteri supplementation can increase the expression of TLR-2 and NOD-2 in gingival epithelium. Experimental design in this study was randomized control group post test only design. Study was carried on 24 white rat (Rattus norvegicus) Wistar strain which divided into 4 groups. Positive control was rats that induced with S. mutans, while rats in negative control group were not induced. Group I was rats that suppelemented with L. reuteri for 14 days (day 1-14) and induced with S. mutans for 7 days (day 8-14). Group II was rats that supplemented with L. reuteri and induced with S. mutans simustaneously for 14 days (day 1-14). Concentration of bacterial suspension was 108 cfu ∕ml for L. reuteri and 1010 cfu/ml for S. mutans. Both of these two bacteria was given orally to rats. TLR-2 and NOD-2 expressions were evaluated with immunohistochemistry technique. Significant differences of protein expression between each treatment groud was analyzed with ANOVA (p=0.001). TLR-2 nd NOD-2 expressions were higher than negative control. It can be conclude that L. reuteri supplementation as probiotic could increase the expression of TLR-2 and NOD-2 in gingival epithelium of rat.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"2 1","pages":"30-34"},"PeriodicalIF":0.0,"publicationDate":"2016-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89008852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MICROBIAL DIVERSITY AND ITS IMPORTANCE IN MICROBIAL GENETIC RESOURCES PRESERVATION AND ITS ROLE IN NATURAL ENVIRONMENTS 微生物多样性及其在微生物遗传资源保存中的重要性及其在自然环境中的作用

Journal of Biological Researches Pub Date : 2016-01-14 DOI: 10.23869/BPHJBR.21.1.20158

L. Sembiring

{"title":"MICROBIAL DIVERSITY AND ITS IMPORTANCE IN MICROBIAL GENETIC RESOURCES PRESERVATION AND ITS ROLE IN NATURAL ENVIRONMENTS","authors":"L. Sembiring","doi":"10.23869/BPHJBR.21.1.20158","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20158","url":null,"abstract":"Biodiversity has been very common to almost every one. However, a comprehensive understanding on the concept of biodiversity might be less common to many discussing parties. Likewise, microbial diversity is even less common to most of academician, ex-cept of course to microbiologists. In fact, a correct and clear concept of biodiversity is prerequisite for serious and appropriate discussion of the matter. In this paper, the concept and understanding of microbial diversity is fundamentally described as well as their genetic potential by reviewing the development and application of species concept based on molecular biological approach. It is an undeniable fact that molecular biology has provided a powerful tool for microbiologists as well as evolutionists to unravel the biodiversity of microbial world which play a paramount important to conserve the basic function of any natural environments in the biosphere since microbes live and flourish in all ecosystems, including extreme habitats. The ubiquity of microbes clearly underpins by their diversity, including physiological and metabolic diversity, ability to live in anaerobic environments, and their small size. Molecular biology development and application in microbiology have transformed the three areas in microbiology, namely microbial ecology, microbial diversity, and microbial evolution from weakness into the strength, in unraveling and understanding microbial diversity and its genetic potential as well as its role in nature, especially their role to keep work the biogeochemical cycle in the Earth. Only by having an adequate understanding of microbial critical role in preserving nature that the environmental conservation issue could be carried out, understood and realized meaningfully.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"20 1","pages":"13-17"},"PeriodicalIF":0.0,"publicationDate":"2016-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79892257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of root and litter on soil macroporosity of Terminalia microcarpa and Dillenia philippinensis in Purwodadi Botanical Garden 普武达地植物园微细叶蝉和菲律宾细叶蝉根系和凋落物对土壤宏观孔隙度的影响

Journal of Biological Researches Pub Date : 2015-12-31 DOI: 10.23869/bphjbr.21.1.201510

A. S. Darmayanti, E. Ariyanti, L. Hakim

引用次数: 1

Speciation and zoogeography of amphibian in Sundaland 巽他兰地区两栖动物的物种形成与动物地理

Journal of Biological Researches Pub Date : 2015-12-31 DOI: 10.23869/BPHJBR.21.1.20159

N. Kurniawan, Driyana Rike Ahmadila, D. S. Nahari, A. Firdaus

引用次数: 2

The prevelence of H5N1 influenza virus on poultry at traditional market in Semarang Indonesia H5N1流感病毒在印度尼西亚三宝垄传统市场家禽中的流行

Journal of Biological Researches Pub Date : 2015-10-10 DOI: 10.23869/BPHJBR.20.2.20151

R. Susanti

引用次数: 1

Tobacco Diversity in Indonesia 印度尼西亚的烟草多样性

Journal of Biological Researches Pub Date : 2015-10-10 DOI: 10.23869/BPHJBR.20.2.20155

D. Djajadi

引用次数: 12

Kenaf: its prospect in Indonesia 红麻:在印尼的前景

Journal of Biological Researches Pub Date : 2015-10-10 DOI: 10.23869/BPHJBR.20.2.20154

E. L. Arumingtyas

引用次数: 5

Δ6 fatty acyl desaturase gene in a southern bluefin tuna (Thunnus maccoyii) cell line Δ6南部蓝鳍金枪鱼(Thunnus maccoyii)细胞系的脂肪酸酰基去饱和酶基因

Journal of Biological Researches Pub Date : 2015-10-10 DOI: 10.23869/bphjbr.20.2.20156

Senni Bunga, K. Schuller

{"title":"Δ6 fatty acyl desaturase gene in a southern bluefin tuna (Thunnus maccoyii) cell line","authors":"Senni Bunga, K. Schuller","doi":"10.23869/bphjbr.20.2.20156","DOIUrl":"https://doi.org/10.23869/bphjbr.20.2.20156","url":null,"abstract":"Fish is the main source of ω-3 long chain polyunsaturated fatty acids (LC-PUFA) such as eicosapentaenoic acid (EPA, 20:5ω-3) and docosahexaenoic acid (DHA, 22:5ω-3), which have positive effects on human health and can be beneficial in human diet context. Studies involving fatty acyl desaturase (Fads) and elongase of very long chain fatty acids (Elovl) enzymes that convert C18 PUFA to C20/22 LC-PUFA have been performed in some fish species. However, very little is known about LC-PUFA biosynthesis in tuna species. This study investigated the Δ6 Fads gene performance in the SBT cell line. The Δ6 Fads nucleotide sequences from various fish species were identified and retrieved to compare them with the SBT Δ6 Fads nucleotide sequence. The Δ6 Fads gene was performed using real time PCR (RT-PCR) and then was compared it with β-actin gene performance as a reference housekeeping gene. By performing multiple sequence alignments and comparing the highly conserved regions among fish Δ6 Fads sequences, the SBT Δ6 Fads nucleotide sequence was determined to be ≥ 75% identical to the other fish Δ6 Fads sequences. The results showed that when the SBT Δ6 Fads and β-actin cDNAs were performed in a standard PCR system and the products were analysed by electrophoresing them on a 2% (w/v) agarose gel, the target genes that were obtained were similar to the expected sizes. The observed band size for the Δ6 Fads PCR product was 207 bp and for the β-actin PCR product was 98 bp. The presence of the observed bands indicated that the primer pairs that were designed and used were successfully amplified the target genes. The results of this study might provide relevant information to support further investigating of the desaturase and elongase gene expression that might contribute to a better understanding of ω-3 LC-PUFA in fish.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"72 1","pages":"33-38"},"PeriodicalIF":0.0,"publicationDate":"2015-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82559343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The patterns of sex determination and differentiation genes in green sea turtle (Chelonia mydas) 绿海龟性别决定与分化基因模式的研究

Journal of Biological Researches Pub Date : 2015-08-01 DOI: 10.23869/BPHJBR.20.2.20153

A. Barlian, N. Vanawati, F. D. Ayuningtyas

{"title":"The patterns of sex determination and differentiation genes in green sea turtle (Chelonia mydas)","authors":"A. Barlian, N. Vanawati, F. D. Ayuningtyas","doi":"10.23869/BPHJBR.20.2.20153","DOIUrl":"https://doi.org/10.23869/BPHJBR.20.2.20153","url":null,"abstract":"Green sea turtle (C. mydas) is one of TSD (Temperature-dependent Sex Determination, TSD) animals which mean that their sex is determined by the egg’s incubation temperature. Genotypic Sex Determination (GSD) homologous genes play a role in TSD process. Until now, research on the pattern of sex determination genes in C.mydas has not been conducted yet. The aim of this research is to reveal sex determination and differentiation genes expression in Mesonephros-Gonad (MG) complexes of C. mydas embryos which incubated in masculinizing temperature (MT) and feminizing temperature (FT). C. mydas eggs were incubated in 3 different stage of TSP (Thermosensitive Period) at masculinizing temperature (26±1 0 C, MT) and feminizing temperature (31±1 0 C FT). Mesonefros-gonad complexes were isolated at Pre-TSP stage (FT at 14 th day, MT at 24 th day), TSP stage (FT at 24 th day, MT at 36 th day) and differentiated stage (FT at 40 th day, MT at 58 th day). RNA from mesonephros-gonad (MG) complexes were converted into cDNA by RT-PCR process. Pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9 and Rspo1 genes expression were analyzed by quantitative Real Time PCR (qPCR) method with β-actin gene as an internal control. Result of this study shown that expression pattern of Sf1, Wt1, Aromatase, FoxL2, Sox9, Wnt4, Fgf9, and Rspo1genes in gonadal embryo of C. mydas were increased during gonadal development stage. Four genes expression patterns (Wnt4, Fgf9, Rspo1, and FoxL2) have shown that these genes have role in sexual differentiation rather than in sexual determination.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"48 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2015-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89783323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Substrate characteristics and its impact on distributionn of mangrove species: a case study in Sungai Barong Kecil in the Sembilang National Park at Banyuasih South Sumatra 基质特征及其对红树林物种分布的影响——以南苏门答腊岛Banyuasih Sembilang国家公园Sungai Barong Kecil为例

Journal of Biological Researches Pub Date : 2015-07-09 DOI: 10.23869/BPHJBR.20.1.20141

Y. Windusari, S. Sarno, E. Saleh, L. Hanum

引用次数: 8