Journal of Biological Researches最新文献

{"title":"ANTIMICROBIAL ACTIVITY OF Streptomyces spp. ISOLATES FROM VEGETABLE PLANTATION SOIL","authors":"I. Isnaeni, I. Kusumawati, Mega Ferdina Suwito, Asri Darmawati, N. Mertaniasih","doi":"10.23869/BPHJBR.21.2.20164","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.2.20164","url":null,"abstract":"Fifteen Streptomyces isolates were isolated from soil in some different location on vegetable plantation at agriculture standard condition. The isolates were assessed for their antibacterial activity against Mycobacterium tuberculosis (MTB) ATCC H37RV and mycobacterial which isolated from Dr. Soetomo Hospital patients in Surabaya. The International Streptomyces Project 4(ISP4) and Middlebrook 7H9 (MB7H9) wwere used as growth or fermentation medium. The screening of inhibition activity was performed using turbidimetry and spot-test on agar medium. Results shown that 33.3% of the isolates (5 isolates) have anti-mycobacterial activities. The first line anti tuberculosis drug rifampicin, (RIF), ethambutol (EMB), isoniazid (INH), and pyrazinamide (PZA) were used as standards or positive controls with concentration 20 ppm. Optical density of crude fermentation broth concentrated from five isolates relatively lower than five anti-tuberculosis drug activity standard, although their activities against some microbial were similar to the standard at spot-test. The most efficient isolate shown anti-mycobacterial activity was Streptomyces B10 which identified as Streptomyces violaceousniger. In addition, fatty acid methyl ester (FAME) profile of gas chromatography-mass spectrometry chromatogram of each isolates were studied and compared \u0000to Streptomyces spp.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"18 1","pages":"69-74"},"PeriodicalIF":0.0,"publicationDate":"2016-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77189270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histologic profile and CD44 expression in breast cancer tissue of balur nano divine kretek volunteers","authors":"Dody Novrial, A. Rosyidah, S. Saraswati, S. Subagjo, S. Sarjadi","doi":"10.23869/BPHJBR.21.2.20163","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.2.20163","url":null,"abstract":"Breast cancer is the most common cancer in woman. Accumulation of free radical in the body is one of cancer risk fac-tors. Since 2009, Research Institute for Free Radicals Shedding Malang has been developing balur nano divine kretek method, combination of balur and divine tobacco smoke to decay free radicals. It has been tested on volunteers with cancer, including breast cancer. This study aimed to assess the effect of balur nano divine kretek method to breast can-cer tissue and CD44 expression. The results showed that 66.67% tumor samples suffered extensive damage (grade 3) and high CD44 expression. The high expression of CD44 in most of the samples may indicate better prognosis. The augmentation for the effectiveness consistency of balur nano divine kretek should be continuously conducted with greater samples and more complete molecular markers such as CD24 and ALDH.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"19 1","pages":"65-68"},"PeriodicalIF":0.0,"publicationDate":"2016-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80627069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE EFFECT OF POLYSACCHARIDES KRESTIN BIOACTIVITY FROM Coriolus versicolor TO ESTRADIOL LEVELS ON Mus musculus ESTROUS CYCLE","authors":"S. Wahyuningsih","doi":"10.23869/BPHJBR.21.2.20162","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.2.20162","url":null,"abstract":"This study aimed to determine the activity of polysaccharide krestin (PSK) from Coriolus versicolor extract to the duration of estrous cycle and estradiol levels. PSK was administrated for 24 days in female Mus musculus, strain Balb/C, 10 weeks old, weight about 25-30 grams. Polysaccharide krestin given in sub-chronic dose. There were 4 groups, i.e K0 (0 mg/kg BW), P1 (15 mg/kg BW), P2 (30 mg/kg BW), and P3 (60 mg/kg BW). Each treatment group contained 7 replications. Data were analyzed using One Way ANOVA and Duncan test at α = 5%. The duration of estrous cycle was determined by vaginal smear methods. The estradiol levels were measured using ELISA kit. The results show that the administration of PSK decreased the duration of estrous cycle with an average of 4.4 days. Polysaccharide krestin dose of 15 and 60 mg/kg BW did not affect the estradiol levels. Decrease of estrous cycle duration was still in the range of normal estrous time between 4-6 days. PSK dose of 15 and 60 mg/kg BW can be used as a therapeutic dose.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"7 1","pages":"61-64"},"PeriodicalIF":0.0,"publicationDate":"2016-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75655672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY OF ADHESIN FROM Aggregatibacter actinomycetemcomitans LOCAL ISOLATE ON ALVEOLAR BONE DESTRUCTION IN AGGRESSIVE PERIODONTITIS DISEASE","authors":"R. D. Ridwan, Tuti Kusumaningsih, Sidarningsih, Soetjipto","doi":"10.23869/bphjbr.21.2.20161","DOIUrl":"https://doi.org/10.23869/bphjbr.21.2.20161","url":null,"abstract":"Adhesion is a powerful survival mechanism as well as a virulence mechanism for bacterial pathogens. Bacterial adhesin is a media for bacteria to invade the host. Bacterial adhesin,is a medium for bacteria to invade the host. Baterial adhe-sion, moreover, is depend on the ligand interaction as a signaling mediator that will influence the invasion process and increase pro and anti-inflammatory due tob the influence of the receptors of innate immune response. Aggregatibacter actimycetemcomitans (A.actinomycetemcomitan) have many virulence factors that may result in tissue and alveolar bone damage. One of the virulence factors is adhesin that can be isolated from the fimbriae. This research purposed to analyze the ability of adhesin protein from A.actinomycetemcomitan that cause the destruction of alveolar bone. Thus, the number of osteoblasts and osteoclasts as well as osteocalcin expression can be used as a marker of damage on the alveolar bone of Wistar rats. The research was conducted through several processes. First, the adhesin of A.actinomycetemcomitan with a molecular weight (MW) of 24 kDa is induced into Wistar rats. Next, to determine the number of osteoblasts and osteoclasts performed, hematoxylin eosin staining is conducted. Meanwhile, to determine osteocalcin expression performed, immunohistochemical techniques is used. This research shows the decreasing of the number of osteoblasts and increasing of the number of osteoclasts in the treatment groups induced by adhesin proteins, A. actinomycetemcomitans + adhesin protein, and A.actinomycetemcomitan compared those in the control group. It also shows the increasing of osteocalcin expressions on the alveolar bone of Wistar rats in the groups induced by adhesin proteins, A. actinomycetemcomitans + adhesin protein, and A. actinomycetemcomitans than those in the control group. It can be concluded that the adhesin protein of A. actinomycetemcomitans plays an important role in the destruction of alveolar bone through the reduction of the number of osteoblasts, the increasing of the number of osteoclasts and oste-ocalcin expression in aggressive periodontitis.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"61 1","pages":"56-60"},"PeriodicalIF":0.0,"publicationDate":"2016-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90609991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE EXPLORATION OF PLANT SPECIES IN NATURE RESERVE OF MOUNT MUTIS EAST NUSA TENGGARA PROVINCE","authors":"S. Solikin","doi":"10.23869/bphjbr.21.1.20151","DOIUrl":"https://doi.org/10.23869/bphjbr.21.1.20151","url":null,"abstract":"This research was aimed to explore and inventory the plant diversity, especially medicinal plants in Nature Reserve of Mount Mutis. Data were collected in Fatumnasi Village, Fatumnasi District of South Central Timor Regency in October 2011 through plant exploration and interview the local people. Plants inventory was conducted along the tracks during exploration. Herbs vegetation analysis was conducted among the tree stands of Eucalyptus urophylla. In addition, orchid vegetation analysis was only conducted to orchids that have been found attaching to Eucalyptus urophylla trees. Results showed that there were about 52 family, 78 genera and 84 species of plants in the observed area. Tree species was dominated by 'ampupu' (Eucalyptus urophylla), while orchid species was dominated by Eria retusa. Herbaceous plant communities were dominated by Centella asiatica, Cyperus sp. and Cynodon dactylon. There were about eight plant species of medicinal plants and one food plant species found in the forestthat have been known by local people.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"5 1","pages":"51-55"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73891188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ANTIBACTERIAL ACTIVITY OF BELILIK (Brucea javanica (L). MERR) AND BENTA (Wikstroemia androsaemofolia DECNE) TO INHIBIT THE GROWTH OF ENTEROPATHOGENIC BACTERIA","authors":"H. Helmi, I. Susanti, Nopian Asmara Agung, S. Kusen","doi":"10.23869/BPHJBR.21.1.20154","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20154","url":null,"abstract":"Several native Indonesia plants have been used to prepare traditional medicine since long time ago. One of common diseases in tropical country is diarrhea, it caused by the infection of enteropathogenic bacteria such as Enteropathogenic Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Shigella sp. Belilik (Brucea javanica (L.) Merr) and Benta (Wikstroemia androsaemofolia Decne) are herbals that utilized as medicine for diarrhea in Bangka Belitung, Indonesia. Parts of these plants are mostly can be utilized as medicine, such as leaf, root, and fruit. The aims of this study were to investigate the antibacterial activity of ethanol crude extract of B. javanica (root and fruit) and W. androsaemofolia (leaf and fruit) against enteropathogenic bacteria (EPEC, P. aeruginoa, S. aureus, Shigella sp.). Method that used was papper disc diffusion. The results showed that at concentration 10 mg/mL, 20 mg/mL, and 30 mg/mL of B. javanica ethanol extract of both root and fruit could not inhibit the enteropathogenic bacteria, while the ethanol extract of leaf and fruit of W. androsaemofolia were shown inhibition activity on the growth of enteropathogenic bacteria. W. androsaemofolia leaf extract performed the best inhibition activity to the growth of EPEC (20.55±1.5mm) and S. aureus (22.14±4.5mm), it was better than kanamycin performance at the same concentration (30 mg/mL). In addition, ethanol extract of W. androsaemofolia fruit showed the best inhibition activity against Shigella sp. (19.64±1.8mm).","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"127 1","pages":"35-40"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81711501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and characteristic of deposit-feeder crabs (Crustacea: Brachyura) in some mangrove ecosystem types in Lombok Island","authors":"D. Murniati","doi":"10.23869/BPHJBR.21.1.20157","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20157","url":null,"abstract":"Lombok Island consists of five different mangrove forest types with various pattern of fauna distribution. These pattern variations were constructed by different type of substrates. Deposit feeder crabs play an important role in determination of substrate pattern. The objective of this study was to investigate the distribution pattern of some crabs which their characteristics are correlated to the mangroves substrate. Crabs were collected from eight locations in Mangrove area of Lombok Island. Ten species of the deposit feeder crab were identified, their distribution pattern was illustrated based on the result of field observation on their morphological characteristics. The highest species diversity found in Teluk Rasu and Tanjung Luar. Both cheliped and maxilliped characteristics represent to habitat preference. Uca annulipes, U. perplexa, U. vocans, and Dotilla myctiroides inhabited sandy substrate, while U. bellator, U. demani, U. dussumieri, U. forcipata, U. triangularis, and Tmethypocoelis ceratophora inhabit muddy substrate. Journal of Biological Research 21(1)","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"1 1","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90846293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric characters of marine local fish (Harpodon sp.) from Tarakan, Northern Borneo","authors":"E. D. Nugroho, D. A. Rahayu, M. Amin, U. Lestari","doi":"10.23869/BPHJBR.21.1.20153","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20153","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"44 1","pages":"41-45"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87194124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of rapd primer for teak (Tectona grandis)","authors":"I. Cintamulya","doi":"10.23869/bphjbr.21.1.20152","DOIUrl":"https://doi.org/10.23869/bphjbr.21.1.20152","url":null,"abstract":"","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"108 1","pages":"46-50"},"PeriodicalIF":0.0,"publicationDate":"2016-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87577846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of protease-producing bacteria isolated from terasi","authors":"N. Arfarita, E. Chukeatirote","doi":"10.23869/BPHJBR.21.1.20155","DOIUrl":"https://doi.org/10.23869/BPHJBR.21.1.20155","url":null,"abstract":"Total of 117 bacterial strains were isolated from terasi samples and 69% of isolates (71) could perform distinctive proteolytic activity that related to the ability to produce protease enzymes. Their proteolytic activity was further tested using spot incubation technique. Strain S4-5 has shown the highest activity then was selected for further tests in this study. Gram staining test showed that S4-5 is gram positive bacteria and able to grow under anaerobic condition. Based on API biochemical profiles, S4-5 strain bacteria was Bacillus licheniformis. Similarity test of genome sequence among Bacillus species from gene bank (EMBL Sequence Version) with Bacillus spp., strain S4-5 had similarity with Bacillus licheniformis genome. The optimal pH of this strain was 6 whereas the optimum temperature for Bacillus licheniformis strain S4-5 was 37oC.","PeriodicalId":31368,"journal":{"name":"Journal of Biological Researches","volume":"3 1","pages":"18-23"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89348720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}