Buletin PeternakanPub Date : 2022-05-30DOI: 10.21059/buletinpeternak.v46i2.72519
B. B. Koten, R. Wea, Rosalin Kana Tiry, Seni Otto, Apriana Wasti Belandina Selan, Welius Yikwa, Allan Prima Titong
{"title":"RESPON ARBILA (Phaseolus lunatus L.) TERHADAP PENAMBAHAN BERBAGAI LEVEL BOKASHI DAUN GAMAL DAN FESES KAMBING","authors":"B. B. Koten, R. Wea, Rosalin Kana Tiry, Seni Otto, Apriana Wasti Belandina Selan, Welius Yikwa, Allan Prima Titong","doi":"10.21059/buletinpeternak.v46i2.72519","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i2.72519","url":null,"abstract":"Penelitian yang bertujuan mengevaluasi respon arbila terhadap penambahan berbagai level bokashi daun gamal dan feses kambing, telah dilaksanakan selama 5 bulan di lahan Politani Kupang. Bahan dan alat yang digunakan adalah benih arbila, daun gamal, feses kambing, timbangan berkapasitas 50 dan 5 kg, EM4, gula pasir dan air. Penelitian ini dirancang dengan rancangan acak lengkap, 4 perlakuan 5 ulangan, terdiri dari P0: tanpa bokashi (control), P5: 5 ton bokashi/ha, P10 : 10 ton bokashi/ha, dan P15: 15 ton bokashi/ha. Variabel yang diukur adalah jumlah bintil akar (bintil), persentase bintil akar efektif (%), serapan nitrogen (g/polibag), diameter batang (mm), pertambahan jumlah daun (daun/minggu), ratio batang dan daun, produksi hijauan (bahan segar (BS), bahan kering (BK), bahan organik (BO) dan produksi protein kasar (PK) hijauan (g/polybag), nilai nutrient (%) (bahan kering (BK), BO, PK, serat kasar (SK), lemak kasar (LK) dan abu). Data dianalisis ragam dan dilanjutkan dengan Uji Duncan. Hasil analisis varians menunjukkan bahwa level bokashi berpengaruh sangat nyata (P<0,01) terhadap pertambahan jumlah daun, produksi BO dan produksi PK hijauan, berpengaruh nyata (P<0,05), terhadap jumlah bintil akar, serapan nitrogen, diameter batang, produksi BS dan BK hijauan, kadar BK, BO, PK, SK, BETN, dan Abu. tetapi tidak berdampak terhadap persentasi bintil akar efektif, ratio batang dan daun serta kadar lemak hijauan arbila. Uji Duncan menunjukkan bahwa perlakuan P15, hasilkan jumlah bintil akar, serapan N, pertambahan jumlah daun, produksi BS, BK, BO dan PK hijauan, kadar BO, PK, dan BETN tertinggi. Disimpulkan bahwa arbila memberikan respon positif terhadap level bokashi berbahan daun gamal dan feses kambing dengan level terbaik adalah 15 ton/ha.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44304209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.67188
F. A. Z. Chairunissa, S. Bintara, T. Hartatik
{"title":"Single Nucleotide Polymorphism of Partial GDF9 Gene in Three Local Goat of Indonesia Compare with Several Goat in Asia","authors":"F. A. Z. Chairunissa, S. Bintara, T. Hartatik","doi":"10.21059/buletinpeternak.v46i1.67188","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.67188","url":null,"abstract":"The GDF9 gene is a gene that affects the maturation of oocytes. GDF9 is expressed in oocytes and granulosa cell, it can stimulate granulose cell proliferation and regulate cumulus cell function from pre-ovulation to ovulation. The GDF9 gene is associated with an increase in the ovulation rate and litter size in animals. This study aims to determine the kinship relationship of local goats compared to goats in Asia on prolific traits and to determine the restriction mapping of the GDF9 gene in goats based on the different SNP locations. The local goat comes from the Bligon goat, Kacang goat and Kejobong goats which is compared to the GenBank data (EF446168, EU883989 and KY780296). GDF9 sequences were analyzed using BioEdit and sequencing results to identify Single Nucleotide Polymorphism (SNP) and using NEBCutter V2 to determine the restriction enzyme which recognized the sequence around SNP. The result shows that three variations of SNP were found in exon 2 (g.3615T>C, g.3760T>C and g.3855A>C). Identification of SNP position found 1 SNP position identified by restriction enzyme at g.3855A> C. The identified restriction enzyme is HpaII and MspI. The results of this study are expected to provide genetic information that will be used for further research on the relationship between GDF9 gene polymorphisms to animal prolific.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44640715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.70339
R. Dewi, K. Kustantinah, M. Muhlisin
{"title":"The Effect of Protected Lemuru Fish Oil Supplementation on In Vivo Nutrient Digestibility and Sheep Blood Profile","authors":"R. Dewi, K. Kustantinah, M. Muhlisin","doi":"10.21059/buletinpeternak.v46i1.70339","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.70339","url":null,"abstract":"This research was intended to observe the effect of protected lemuru fish (Sardinella longiceps) oil for ruminants, especially for sheep. This study aimed to evaluate the digested nutrients and blood profile of sheep. This study was conducted in September-October 2020 in Dumbira Farm, Kalasan, Yogyakarta, using 12 sheep divided into 3 treatments with 4 replications each. Treatment P0 was Total Mixed Ration (TMR) without protected Lemuru fish oil (control), treatment P1 was TMR with 5% protected Lemuru fish oil, and treatment P2 was TMR with 10% protected Lemuru fish oil. The data were statistically analyzed using one way analysis of variance and continued with Duncan new Multiple Range Test for significant results. The results of this study indicated that the addition of 10% protected Lemuru fish oil in TMR feed had a significant effect (P<0,05) on the increased value of in vivo digestion of crude fiber and crude fat, but did not affect the digestibility of dry matter, organic matter, and protein. The addition of protected Lemuru fish oil did not cause hematological disorders showed by the blood profiles were in the normal range. In conclusion, protected lemuru fish oil supplementation had a favorable influence on the production performance without affecting blood profile of sheep.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47273290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.69852
S. Syafwan, Y. Yatno, Ravido Multer Mahulae, Abraham Lincoln, Deby Isabela BR Sembiring
{"title":"Requirements of Energy and Protein for Arabic Chicken Hens During Late Egg Production Period","authors":"S. Syafwan, Y. Yatno, Ravido Multer Mahulae, Abraham Lincoln, Deby Isabela BR Sembiring","doi":"10.21059/buletinpeternak.v46i1.69852","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.69852","url":null,"abstract":"The present study aimed to estimate the metabolizable energy (ME) and protein (CP) requirements of Arabic chicken hens during the late egg production period reared under a semi-scavenging system with free-choice feeding. A total of 112 sixty-two-week-old Arabic chicken hens were used. The treatments were control and the choice diet consisted of 6 replicate pens. Control hens received a control diet (2750 kcal of ME/kg and 14.1% of CP) complying with the Hy-line Brown Commercial Management Guide 2011, whereas the choice hens offered control and three other diets (high energy-high protein [3006 and 17.3], high energy-low protein [3089 and 12.7], and low energy-high protein [2656 and 17.0] kcal of ME/kg and % of CP, respectively). Feed, ME, and CP intake, the concentration of dietary ME and CP, and egg production were recorded weekly. Data were analyzed using Proc Mixed of SAS. The feeding method influenced feed intake, CP concentration, and ME concentration but had no significant effect on CP intake, ME intake, and egg production. Weekly feed intake of choice hens was lower than that of control hens (514.03 vs. 551.18 g /hen/week; P<0.03). Dietary concentrations of ME and CP in the choice hens were higher than those in the control hens (2957 vs. 2750 kcal of ME/kg; P<0.001 and 150.6 vs. 14.1 g of CP/kg; P<0.001). Egg production of the choice hens was not significantly higher than that of the control hens (51.17% vs. 46.82%; P>0.05). Feed intake, CP intake, and ME intake decreased significantly at week 66 onward, while egg production decreased at week 65 onward. It can be concluded that Arabic chicken hens in the late egg production period were able to adjust their energy and protein requirements by consuming more from high dietary energy than from a high dietary protein. Based on the choice feeding, ME and CP requirements for Arabic chicken hens during the late egg production period in the semi-scavenging system were 2957 kcal/kg and 151 g/kg and higher than ME and CP contain in the control diet of 2750 kcal/kg and 141 g/kg to maintain egg production. The egg mass and feed conversion ratio were better in the choice hens group.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47988873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.70195
I. Ismoyowati, E. Tugiyanti, D. Indrasanti, N. Setianto, V. Armelia
{"title":"Developing Strategy to Reduce the Mortality of Native Chicken using Qualitative Modeling","authors":"I. Ismoyowati, E. Tugiyanti, D. Indrasanti, N. Setianto, V. Armelia","doi":"10.21059/buletinpeternak.v46i1.70195","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.70195","url":null,"abstract":"Smallholder native chicken farming continues to face challenges that include simple farming management as well as ND and AI diseases that lead to decreased productivity and increased mortality rate. The aim of the study was to develop a strategy to reduce the mortality rate of native chickens in extensive and semi-intensive rearing systems. This study uses survey method with 78 extensive and 88 semi-intensive native chicken farmers as respondent. This study explores the disease incidence, illness treatment, mortality rate, as well as AI and ND antibody titers which then analyzed descriptively. System dynamic model using Ventana software (VENSIM) was used to identify the contributing factors to the mortality rate of native chicken in smallholder farming. The results showed that the common diseases among native chickens reared in semi-intensive and extensive farming are AI, ND, CRD, and pullorum, with a high rate of disease-specific mortality (>5%). Compared to native chickens in semi-intensive farming, those of in extensive farming showed a higher natural immunity against AI and ND. The qualitative modeling produced seven reinforcing loops and five balancing loops. Some challenges in developing native chicken farming were disease incidence due to lack of proper land and cage, the occurrence of selling unhealthy chickens, farmers opting out for poultry vaccination, high operational cost, lack of business motivation, limited knowledge on poultry management and health, lack of extension programs, and traditional management. We concluded that the rate of disease-specific mortality (ND and AI) remained high in native chickens reared both in extensive and semi-intensive farming. It takes an effort to improve farming management, vaccination, and the government’s contribution through extension programs to decrease disease incidence and mortality rate of native chickens.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42466392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Productivity of Sentul and Kampung Chickens until the Age of 3 Months in the First Generation Selection Population (G1)","authors":"Wahyuni Wahyuni, Depison Depison, Gushariyanto Gushariyanto","doi":"10.21059/buletinpeternak.v46i1.67045","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.67045","url":null,"abstract":"This study aimed to compare the productivity of Sentul chickens and first-generation Kampung chickens (G1) until the age of 3 months. The research material were Sentul chicken and Kampung chicken. The method used was an experiment method or direct observation with a sample of 174 chickens from each strain. The data collected were egg weight, doc weight, body weight, body weight gain, body measurements, and selection response. Data on egg weight, body weight, and body measurements were analyzed using the average difference test (t-test), while the average value vector of body measurements Sentul chicken and Kampung chicken were analyzed using the T2-Hotelling statistical test. To identify the body size and body shape characteristics of Sentul chickens and Kampung chickens were analyzed using principal component analysis. Data processing used the statistical software Minitab version 18. The results of this study showed that egg weight, body weight at the age of DOC-3 months and body measurements of Sentul chickens were significantly different (P<0.05) higher than the Kampung chickens. This study concludes that the egg weight, body weight, body weight gain, and body sizes of Sentul chickens are higher than Kampung chickens. The size characteristic of Sentul and Kampung chickens is chest circumference. The characteristic of the shape of the Sentul chicken is the length of the wings, while the shape of the native chicken is the width of the chest. The selection response and the heritability value of the Sentul chickens was higher than Kampung chikens. ","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41634688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.67523
Diahanvika Tri Sarvinda, S. Bintara, I. Budisatria, K. Kustantinah, E. Baliarti
{"title":"The Effect of Flushing Premating with Spirulina Platensis Supplementation on Ewes Postpartum Estrus","authors":"Diahanvika Tri Sarvinda, S. Bintara, I. Budisatria, K. Kustantinah, E. Baliarti","doi":"10.21059/buletinpeternak.v46i1.67523","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.67523","url":null,"abstract":"Lactating ewes require high nutrients for basic life requirements and milk production. If not fulfilled, it can have an impact on Negative Energy Balance (NEB) that reduces body weight, Body Condition Score (BCS), and extend the appearance of Postpartum Estrus (PPE). Premating flushing feed is an effort to improve ewe nutrients by adding high nutrition for preparation before mating so that after lambing and suckling, the ewe immediately estrus. This research aimed to evaluate the performance of ewes through premating flushing feeding. The research was conducted at Mendo Galak Farm, Sleman, Yogyakarta. Twenty ewes 2-3 years old with BCS 2-3 divided into two groups; the group with flushing treatment consisted of dried water spinach (Ipomoea reptans poir), concentrate feed with Spirulina sp. (14,92% crude protein, 60,28% total digestible nutrients), and the control group (PS) without Spirulina sp. (crude protein 11,82%, total digestible nutrients 53,20%). Flushing feed was given after a month postpartum as much as 3% dry matter of body weight. The recorded parameter was daily feed consumption, monthly body weight, BCS, and postpartum estrus. The data obtained were tested by an independent T-test with Statistical Product and Service Solution (SPPS ver. 22). The results showed the consumption and digestibility of CP, TDN, and ewe's ADG had a significant difference (P<0.05). Postpartum estrus (PPE) of flushed ewes had no significant difference (P>0.05), 73.90±11.55 vs. 77.60±14.65 days, respectively. The conclusion was that flushing premating treatment with the addition of Spirulina platensis increased the nutrient intake and digestibility of CP, TDN, and ADG but had not shortened on postpartum estrus of lactating ewes.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43112093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.69012
Y. Yendraliza, M. Ridho, M. Rodiallah, Zumarni Zumarni
{"title":"The Quality of Buffalo Sperm Following Preservation Using Different Diluents and Sperm Concentrations","authors":"Y. Yendraliza, M. Ridho, M. Rodiallah, Zumarni Zumarni","doi":"10.21059/buletinpeternak.v46i1.69012","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.69012","url":null,"abstract":"Artificial Insemination (AI) success depends on the quality of the frozen semen. The quality of the frozen semen of swamp buffalo in Indonesia is still low. The study was conducted to determine the quality of buffalo sperm following freezing using three different diluents and three different doses. The study used buffalo semen from the Tuah Sakato Artificial Insemination Center, Payakumbuh (n = 3). The semen collecting was carried out once a week for 10 weeks (replication). The research method used was 3x3 factorial randomized block design. The first factor was diluent (Triladyl®, Andromed® and Tris egg- yolk) and the second factor was the dose of spermatozoa (10 and 15 and 20 x 106 sperm/ml). Data were analyzed using variant analysis, while the differences between treatments were tested by Duncan Multiple Range Test. The results showed that the plasma membrane integrity of buffalo sperm was found in Andromed® diluent, while tris egg-yolk diluent gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. It was concluded that andromed® diluent and tris egg-yolk gave better motility, viability, plasma membrane integrity and recovery rate at a sperm concentration of 20 x106 sperm/mL compared to triladyl® diluent and a sperm concentration of 10 and 15 x106 sperm/mL. of Buffalo of sperm abnormalities not sicnificantly by the type of diluent but are influenced by sperm concentration.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45991423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.70685
Yahya Nor Arif, Nurul Fatimah Isnaniyah, A. Pramono, J. Riyanto
{"title":"To Determine the Effect of Using a Complete Feed Containing Protected Soybean Groats in the Production of Javanese Thin-Tailed Male Sheep Carcasses","authors":"Yahya Nor Arif, Nurul Fatimah Isnaniyah, A. Pramono, J. Riyanto","doi":"10.21059/buletinpeternak.v46i1.70685","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.70685","url":null,"abstract":"The present study aims to determine the impact of complete feed containing protected soybean meal on the production of male thin-tailed lamb carcasses. A total of 15 (23.43 ± 1.40 kg) 12 months old male Thin-Tailed Sheep (TTL) were given 3 treatments and 5 replicates in a completely randomized design. The ration consisted of complete feed and formaldehyde-protected soybean groat (PSG). The treatments includes 100% complete feed (F1), 90% complete feed + PSG 10% (F2), and 80% complete feed + PSG 20% (F3). Data were analyzed using ANOVA and differences between treatments were further tested using Duncan’s New Multiple Range Test. The results indicated that the use of PSG containing complete feed did not improve the characteristics of carcasses (weight and percentage of carcass components, half-cuts and commercial cuts of carcass, fleshing index and meat bone ratio) of male TTLs (P>0.05). However, differences were found in the rib eye muscle area. The use of complete feed containing 20% PSM (F3) resulted the highest rib eye muscle area figure compared to those of F2 and F1 (35.17 vs 27.00 and 26.33; P<0.05). Our study revealed that the use of complete feed containing 20% protected soybean groat resulted in higher rib eye muscle area by 25.14% compared to those containing no protected soybean groat. However, no differences in carcass characteristics were found in view of weight and percentage of carcass components, half cut and commercial carcass, fleshing index and meat bone ration in thin-tailed sheep.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43495825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Buletin PeternakanPub Date : 2022-02-27DOI: 10.21059/buletinpeternak.v46i1.69784
Winni Liani Daulay, Putri Indah Ningtias, C. Sumantri, J. Jakaria
{"title":"Expression of Myostatin Gene in Belgian Blue and Ongole Grade Crossbred Cattle","authors":"Winni Liani Daulay, Putri Indah Ningtias, C. Sumantri, J. Jakaria","doi":"10.21059/buletinpeternak.v46i1.69784","DOIUrl":"https://doi.org/10.21059/buletinpeternak.v46i1.69784","url":null,"abstract":"Investigating Myostatin (MSTN) as a potent inhibitor of skeletal muscle growth and development to produce excessive muscles is extremely essential for livestock breeding. This study aimed to analyze the expression of the MSTN gene and its relationships with genotype and phenotype (normal-muscled vs double-muscled) of Belgian Blue (BB) x Ongole Grade (PO) crossbred cattle. For that purpose, 12 animals from BB, PO, BB x PO F1, and BB x PO F2 cattle (3 animals each) raised at Balai Embrio Ternak (BET) Cipelang Bogor, West Java were used for blood sample collection. Genotyping analysis was performed using the PCR-RFLP method withprimer F: 5’-CTC TTC TTT CCT TTC CAT ACA GAC-3’ and R: 5’-AGG GGA AGA CCT TCC ATG TT-3’, while the MSTN gene expression was analyzed using the qPCR technique. As results, three genotypes: del.11/del.11, +/del.11, and +/+ were detected. The del.11/del.11 genotype, which showed a double-muscled phenotype was found in BB cattle and BB x PO F2 cattle. The +/del.11 genotype was found in BB x PO F1 cattle and BB x PO F2 cattle. The +/+ genotype, which showed a normal phenotype was only detected in PO cattle. There was a significant difference of the MSTN gene expression in the sampled animals among genotypes and between phenotypes (normal-muscled vs double muscled). The MSTN expression in animals with del.11/del.11 genotype was higher than that in animals with +/del11 and +/+ genotypes (P<0.05). Animals with +/+ genotype showed the lowest MSTN expression. It was concluded that double-muscled animals showed higher MSTN expression than normal-muscled animals.","PeriodicalId":31305,"journal":{"name":"Buletin Peternakan","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48724206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}