Proceedings of 5th International Electronic Conference on Medicinal Chemistry最新文献

{"title":"Preliminary phytochemical analysis and in vitro anti-cancer screening of ethanolic extract of roots of Bauhinia tomentosa L.against HUH-7 human liver cancer cell lines","authors":"Ramaiah Maddi","doi":"10.3390/ecmc2019-06275","DOIUrl":"https://doi.org/10.3390/ecmc2019-06275","url":null,"abstract":"The most effective way to reduce the worldwide burden of liver cancer is to prevent it from happening in the first place. The current treatment of liver cancer has significant side effects. Hence, there is a need to develop anti-liver cancer agents of plant origin, which are less toxic, more efficacious and cost-effective. The present study has been performed experimentally by in vitro to examine the anti-liver cancer activity of roots of Bauhinia tomentosa L (Fabaceae). The roots of B. tomentosa was tested for its anti-cancer activity against HUH-7 human liver cancer cell lines by MTT assay. The standard used in this assay was Camptothecin (CPT) at 25µG concentration. Plant extract was tested at 25μg/mL, 50 μg/mL,100μg/mL, 200 μg/mL and 400 μg/mL concentrations. The percent cell viability of standard drug was found to be 49.59% and plant extracts at 25 μg/mL, 50 μg/mL,100 μg/mL, 200 μg/mL and 400 μg/mL concentrations were found to be 93.82%, 86.21%, 74.48%, 63.04%, 45.71% respectively. The cell morphology was observed and recorded under a microscope. The results clearly indicated that B. tomentosa shows a dose-dependent activity and it was maximum at 400μg/mL concentration where it shows 45.71% of liver cancer cell viability and it was comparable to the standard drug where it shows 49.59% of viability.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131876559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharma-toxicological and phytochemical investigations on Harpagophytum procumbens DC. ex Meisn. water extract: potential application in colon inflammation","authors":"G. Orlando","doi":"10.3390/ecmc2019-06283","DOIUrl":"https://doi.org/10.3390/ecmc2019-06283","url":null,"abstract":"Inflammatory bowel diseases (IBDs) are chronic, relapsing and multifactorial disorders of the colonic mucosa, which show increased and unbalanced intestinal immune response to external stimuli. Plant-derived extracts were described to possess the capability in contrasting IBDs-related oxidative stress and inflammatory pathways. In the present study, we investigated the water extract of Harpagophytum procumbens DC. ex Meisn. in an experimental model of IBD. Additionally, a microbiological investigation was carried out to discriminate the efficacy against bacterial and fungal strains involved in IBDs. An untargeted proteomic analysis was also conducted on more than 100 colon proteins involved in tissue morphology and metabolism. The extract showed the ability to blunt the level of selected biomarkers of oxidative stress and inflammation, including serotonin, prostaglandins, cytokines and transcription factors. Additionally, the extract inhibited the growth of Candida albicans and C. tropicalis, in vitro. The extract was also able to exert a pro-homeostatic effect on the levels of a wide plethora of colon proteins, thus corroborating protective effects against the burden of inflammation and oxidative stress. On the other hand, the supra-physiological downregulation of cytoskeletal-related proteins involved in tissue morphology and antimicrobial barrier function suggests caution in the use of food supplements enriched with H. procumbens.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"202 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132428378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of cancer-associated glycobiomarkers using lectin-based biosensors","authors":"M. L. Silva","doi":"10.3390/ecmc2019-06280","DOIUrl":"https://doi.org/10.3390/ecmc2019-06280","url":null,"abstract":"Cancer is a group of diseases characterized by a wide diversity in location, cellular features and aggressiveness. Nonetheless, a common aspect seen in different types of cancer, namely in carcinomas, is the alteration in post-translational modifications of proteins, particularly in protein glycosylation [1]. Due to increased or decreased expression of particular enzymes participating in glycosylation of proteins, different glycan structures are formed, which are typical of tumoral cells. When the respective glycoproteins are secreted into the blood stream, these aberrant structures can be used as valuable cancer biomarkers, since they are not synthesized by normal cells. \u0000Glycan structures are efficiently and selectively detected by lectins, which are proteins of natural origin with high affinity for a particular or a very small group of glycan epitopes. Lectins are used for glycan detection as antibodies are used for protein recognition. Therefore, they have been employed in diverse analytical techniques when the aim is to selectively detect or capture specific glycans from a complex sample. \u0000Lectin biosensors are attractive devices for the detection of cancer-associated glycobiomarkers in serum since they combine the advantageous aspects of biosensors (portability, easy use in point-of-care analysis, low sample requirement) with the high selectivity of lectin biorecognition. This work presents three lectin-based impedimetric biosensors for the selective detection of specific aberrant cancer-associated O-glycans, namely STn, Tn and T antigens, which are well-established pan-carcinoma biomarkers. For these three biosensors, Sambucus nigra agglutinin, Vicia villosa agglutinin and Arachis hypogeae agglutinin were used as biorecognition elements, with specificity for STn, Tn and T antigens, respectively. The binding event between each lectin and the corresponding aberrant O-glycan was monitored by electrochemical impedance spectroscopy, measuring the increase in the biosensor’s impedance after incubating the samples. The increase in impedance was related to the lectin-glycan complex formation. \u0000All biosensors were constructed following the same general procedure, demonstrating its high versatility. A thorough characterization and validation of the biosensors’ performance was carried out, evaluation their selectivity, sensitivity and ability to discriminate between samples from healthy donors and from cancer patients with different carcinomas. Using the three lectin biosensors in the analysis of the same sample could also help to characterize the glycosylation profiles of glycoproteins in the diverse types of carcinomas. \u0000 \u0000[1] Pinho S.S., Reis C.A. Glycosylation in cancer: mechanisms and clinical implications. Nat. Rev. Cancer 15 (2015) 540-555.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128868152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic strategies towards bioactive nature-inspired indole-containing alkaloids","authors":"S. Long, D. Resende, P. Pereira-Terra, Joana Freitas-Silva, Artur M. S. Silva, M. Tiritan, A. Kijjoa, E. Pinto, Paulo M. Costa, M. Pinto, E. Sousa","doi":"10.3390/ecmc2019-06291","DOIUrl":"https://doi.org/10.3390/ecmc2019-06291","url":null,"abstract":"Currently drug resistance is rising to dangerously high levels worldwide and threatening our ability to treat even common infectious diseases. Secondary metabolites, especially alkaloids containing an indole group and structurally related to fumiquinazolines, are of crucial importance in the area of drug discovery, having representatives such as fiscalin B that was reported as substance P antagonist and neofiscalin A, a potent antibacterial agent active in both reference and multidrug-resistant isolates. [1] Herein, the synthesis of quinazolinone alkaloid derivatives containing an indole moiety is reported, using two different methodologies – a highly efficient three-component one-pot microwave-assisted and a multi-step Mazurkiewicz-Ganesan approach. With this approach, 38 derivatives were obtained in low to moderate yields and were further tested for their antitumor [2,3], neuroprotection [2], antibacterial, and antifungal activities. While 16 compounds exhibited weak to moderate tumor cell growth inhibitory activity, other four compounds showed potential for in vitro neuroprotection in Parkinson disease. It was also observed for some derivatives a good antibacterial activity against clinical Staphylococcus aureus resistant to methicillin (MRSA). Structure-activity relationship was established and four hit compounds containing the quinazolinone scaffold emerged as potential drug candidates. \u0000 Acknowledgements: We thank the UID/Multi/04423/2019 through national funds provided by FCT-Foundation for Science and Technology and European Regional Development Fund (ERDF), in the framework of the program PT2020. This research was developed under Project No. POCI-01-0145-FEDER-028736, co-financed by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT through national funds, and Solida Long thanks Erasmus-Lotus+ program (LOTUS+, LP15DF0205). \u0000References: \u0000[1] Resende, D. I. S. P.; Boonpothong, P.; Sousa, E.; Kijjoa, A.; Pinto, M. M. M., Chemistry of the fumiquinazolines and structurally related alkaloids. Natural Product Reports 2019, 36, 7-34. \u0000[2] Long, S.; Resende, D. I. S. P.; Kijjoa, A.; Silva, A. M. S.; Fernandes, R.; Xavier, C. P. R.; Vasconcelos, M. H.; Sousa, E.; Pinto, M. M. M., Synthesis of New Proteomimetic Quinazolinone Alkaloids and Evaluation of Their Neuroprotective and Antitumor Effects. Molecules 2019, 24 (3), 534. \u0000[3] Long, S.; Resende, D.; Kijjoa, A.; Silva, A.; Pina, A.; Fernandez-Marcelo, T.; Vasconcelos, M.; Sousa, E.; Pinto, M., Antitumor Activity of Quinazolinone Alkaloids Inspired by Marine Natural Products. Mar. Drugs 2018, 16 (8), 261.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127902576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumption of medicinal plants for central nervous system (CNS) disorders among university students in the Community of Madrid","authors":"M. Gómez-Serranillos, E. González-Burgos, I. Iglesias, R. Lozano, M. Gómez-Serranillos","doi":"10.3390/ecmc2019-06315","DOIUrl":"https://doi.org/10.3390/ecmc2019-06315","url":null,"abstract":"","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121299533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of new 2-heteroaryl-4-quinolones as potential antibiotics targeting multi-​drug resistant ESKAPEE pathogens","authors":"Marine Duplantier, Elodie Lohou, P. Sonnet","doi":"10.3390/ecmc2019-06324","DOIUrl":"https://doi.org/10.3390/ecmc2019-06324","url":null,"abstract":"","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"322 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122988557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of pharmacokinetic properties of CK2 Inhibitors with an Indeno[1,2-b]indole scaffold","authors":"Robin Birus, M. B. Borgne, J. Jose","doi":"10.3390/ecmc2019-06335","DOIUrl":"https://doi.org/10.3390/ecmc2019-06335","url":null,"abstract":"","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122545359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of high valued medicinal compounds using plant cell tissue and organ culture","authors":"M. Adil","doi":"10.3390/ecmc2019-06308","DOIUrl":"https://doi.org/10.3390/ecmc2019-06308","url":null,"abstract":"One slide, Max 200 words Today market demand for natural products is high and the conventional practices to get the herbs from wild nature or cultivated fields are not sufficiently efficient to meet the demand. Alternative approaches such as plant tissue organ and cell cultures (PTOC) are believed to be promising and inexpensive methods. The PTOC approach offers the opportunity to sustain the standardized natural products of uniform quality and ensures to be free of agrochemicals, toxins or other environmental pollutants. Number of studies using in vitro tissue culture approaches have extensively been studied and adopted for uniform and continuous supply of natural products. There are companies taking advantages of this technology, which offers the opportunity to produce medicinal compounds continuously and in a limited space rather than cultivating on hectares of land. Despite these advantages the PTOC system needs optimization and there are factors regulating plant cell machinery to process primary metabolites for the secondary metabolites (medicinal compounds) production. These factors are plant growth regulators (PGRs), substrate type and concentration, light condition, elicitors and precursors feeding. Additionally, the bioreactor design also plays important role to ensure the large scale production using plant cells and organs cultures.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122981212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetics of Gestobutanoil in rat serum using HPLC-APCI-MS","authors":"E. Stepanova, L. Makarenkova, V. Chistyakov","doi":"10.3390/ecmc2019-06327","DOIUrl":"https://doi.org/10.3390/ecmc2019-06327","url":null,"abstract":"Pilot studies of the pharmacokinetics of Gestobutanoil (GB) showed that it undergoes rapid biotransformation forming two metabolites, which quantitative analysis was significant. An HPLC-MS technique was developed for the simultaneous quantification of GB and its two metabolites in rat serum. Due to the nature of GB and one of its metabolites, the ESI-MS-detection was ineffective. The ionization problem was solved with APCI. The detection of GB and one of its metabolites was carried out by the fragments of their molecules formed in the ionization chamber. The second metabolite formed [M+H]+ ions. During the method validation following characteristics were checked out: specificity, linearity, Precision, Accuracy, matrix effect, stability. The limit of quantification for each analyte was 10 ng/ml. Pharmacokinetics studies have shown that biotransformation of GB is so fast that it wasn't detected in rat serum even in 15 minutes after administration. The pharmacokinetics of two metabolites of GB was described.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126324154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 3’UTR of the West Nile Virus genomic RNA is a potential antiviral target site","authors":"S. Ramos-Lorente, Isabel Pérez-Jover, C. Romero-Lopez, A. Berzal-Herranz","doi":"10.3390/ecmc2019-06281","DOIUrl":"https://doi.org/10.3390/ecmc2019-06281","url":null,"abstract":"The protein coding-information only represents a small portion of the genetic load of a living organism. It is well established that essential information codes functional RNAs, called non-coding RNAs (ncRNAs), which play key roles in the essential biological processes of the cell life. Many mRNAs also act as truly ncRNAs besides being translated into proteins. Therefore, the repertoire of potential drug targets to fight diseases goes beyond proteins. Viral RNA genomes encode all the information for completion of the infectious cycle. They are multifunctional molecules, which act as replication templates and mRNAs. Further, defined structural domains in viral RNA genomes play key functions for the completion of the viral cycle and the regulation of the essential processes; these domains have also been involved in virulence. The West Nile Virus (WNV) genome consists in a single stranded RNA molecule, which contains a single ORF flanked by untranslated regions (UTRs). The 3’UTR is required for efficient translation, but the mechanisms involved in this regulation are still obscure. In this work, we show evidences that the WNV-3’UTR specifically recruits the 40S ribosomal subunit. We have localized two potential binding sites of the 40S. Binding of the 40S induced conformational changes in highly conserved structural domains within the WNV-3’UTR. Functional assays support the hypothesis that recruitment of the 40S particle by the 3’UTR is required for an efficient translation. Interfering with the 40S recruitment, by targeting the WNV-3’UTR binding sites, constitutes a potential antiviral strategy by the development of new therapeutic compounds.","PeriodicalId":312909,"journal":{"name":"Proceedings of 5th International Electronic Conference on Medicinal Chemistry","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128695695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}