{"title":"Association Between Host Gut Microbiota and SARS-CoV-2 Infection","authors":"F. Firoozeh","doi":"10.34172/ijep.2020.31","DOIUrl":"https://doi.org/10.34172/ijep.2020.31","url":null,"abstract":"<jats:p>\u0000 </jats:p>","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77105926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Niloofar Ghomimaghsad, Somayeh Yaslianifard, M. Mohammadzadeh, M. Dadashi, M. Noorisepehr
{"title":"Contamination of Water Sources of Karaj Hospitals with Legionella pneumophila and Campylobacter jejuni","authors":"Niloofar Ghomimaghsad, Somayeh Yaslianifard, M. Mohammadzadeh, M. Dadashi, M. Noorisepehr","doi":"10.34172/ijep.2020.29","DOIUrl":"https://doi.org/10.34172/ijep.2020.29","url":null,"abstract":"Background: One of the most common routes of infection development in humans is contaminated water. Legionella pneumophila and Campylobacter jejuni are the important causes of community- and hospital-acquired pneumonia and gastroenteritis that are transmitted to humans via the inhalation of contaminated water droplets and consumption of contaminated water, respectively. Thus, continuous monitoring of the water supply systems for these pathogens has great importance in public health. Objective: This study aimed to evaluate the water contamination of Karaj hospitals with these two bacterial species. Materials and Methods: In this study, 62 water samples were obtained from different parts of the hospitals of Karaj from April to September 2019, including air conditioning systems, dialysis equipment, ventilation tanks, and different wards of a hospital such as infectious diseases, pediatrics, gastroenterology, dialysis, and intensive and neonatal intensive care units. The samples were collected in sterile containers and immediately transferred to the laboratory for further analysis. The culture on specific media, staining, and biochemical tests were performed to identify the L. pneumophila and C. jejuni. Results: Out of 62 water samples, 25.8% (16 samples) were positive for L. pneumophila; 68.75% were observed in hot water samples, and 31.25% were attributed to cold water samples. Among 62 samples, 4.84% (3 samples) were positive for C. jejuni, which were all detected in hot water samples. Conclusion: Considering that the methods of water refinery of municipal water have no high efficiency, the quality improvement of the water sources of hospitals seems to be necessary.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79786338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Poonam G. Daswani, V. Muthuraman, R. Macaden, M. Dias, T. Birdi
{"title":"Effect of Psidium guajava (guava) L. Leaf Decoction on Antibiotic-resistant Clinical Diarrhoeagenic Isolates of Shigella spp.","authors":"Poonam G. Daswani, V. Muthuraman, R. Macaden, M. Dias, T. Birdi","doi":"10.34172/ijep.2020.26","DOIUrl":"https://doi.org/10.34172/ijep.2020.26","url":null,"abstract":"Background: Although shigellosis is self-limiting, antibiotics are recommended to minimize the severity of symptoms and reduce mortality rates. However, due to the increasing reports of antibiotic resistance, alternative approaches are needed to combat shigellosis. Interest for research on medicinal plants has increased in recent years, and hence, they can be explored to treat this infectious diarrhoea. Objective: To study the effect of Psidium guajava L. (guava) leaf decoction (GLD) on the antibiotic-resistant clinical isolates of Shigella spp. Materials and Methods: A total of 43 isolated Shigella spp. from diarrhoeal patients were used in this study. The effect of GLD on the bacterial viability was initially assessed. The isolates were divided into two categories: sensitive and resistant to GLD. For sensitive isolates, antibacterial activity of GLD was evaluated while for resistant strains, the ability of GLD for reducing the bacterial invasion of the HEp-2 cell line underwent an investigation. Results: Among the 43 Shigella isolates, GLD affected the growth of 23 strains. The invasion of 9 strains from the 20 remaining resistant isolates was unaffected. Although the number of isolates was less, the data suggested that isolates belonging to S. flexneri serogroup were more sensitive to GLD in comparison with other spp (i.e., sonnei, boydii, and dysenteriae). Conclusion: The results of this study revealed the efficacy of GLD against drug-resistant Shigella spp. and thus could be considered for the treatment of diarrhoea. GLD can be a cost-effective alternative to antibiotics.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"33 7-8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77658646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amir Karimipour-Saryazdi, F. Ghaffarifar, Pooya Tavakoli, Yeganeh Karimipour-Saryazdi, Leila Zaki, S. Bahadory
{"title":"Anti-parasitic Effects of Herbal Extract-Based Silver Nanoparticles on the Trophozoite and Cystic Forms of Acanthamoeba Protozoa","authors":"Amir Karimipour-Saryazdi, F. Ghaffarifar, Pooya Tavakoli, Yeganeh Karimipour-Saryazdi, Leila Zaki, S. Bahadory","doi":"10.34172/IJEP.2020.19","DOIUrl":"https://doi.org/10.34172/IJEP.2020.19","url":null,"abstract":"Background: Acanthamoeba is a globally dispersed protozoan that can cause different clinical manifestations in infected individuals. Various drugs have been proposed against its drug-resistant forms. Objective: The present study examined silver nanoparticles (NPs) with a good anti-parasitic background. More precisely, the study focused on evaluating the anti-parasitic effect of silver nano-scale particles on protozoan trophozoite and cysts by microscopic counting and flow cytometry after exposure to different concentrations. Methods: To this end, MTT assay and IC50 were used to assess the macrophage toxicity and cysts/trophozoites, respectively. Results: Based on the results, 100 ppm silver NPs had better anti-parasitic effects than 80 ppm concentration and even the standard treatment of Acanthamoeba on both trophozoite and cystic phases. Macrophages toxicity at 100 ppm concentration was similar to the control group. Conclusion: In general, further studies should be conducted to confirm the present results given the significant effects of silver NPs against trophozoite and parasite cysts.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"22 1","pages":"84-88"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86544636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inhibitory Effect of Different Types of Fermented Milk on Candida albicans","authors":"Maryam Azizkhania, P. Sarisb, Mehdi Baniasadic","doi":"10.34172/IJEP.2020.21","DOIUrl":"https://doi.org/10.34172/IJEP.2020.21","url":null,"abstract":"Background: Candida albicans (C. albicans) is known as an opportunistic fungal pathogen, and although it is a normal flora of the gastrointestinal tract, it has the ability to colonize every human tissue, causing serious and invasive infections. Objective: This study focused on the antifungal activity of the produced yogurts and kefirs from cow, camel, sheep, and goat milk against C. albicans during the storage period at 4 o C. Materials and Methods: The pH, titratable acidity, the content of some organic acids, and anti-Candida activity of yogurt and kefir samples were evaluated based on the aim of the study. Results: The titratable acidity of the samples significantly increased after the fermentation, along with pH reductions. The concentration of lactic and pyruvic acids increased during the fermentation while citric, uric, and hippuric acid content demonstrated a decrease. The results revealed that kefir samples had a stronger anti-Candida effect compared to yogurt samples. On the first day of the storage period, the growth inhibitory effect of sheep milk kefir was 90.20%, followed by camel kefir (78.37%), goat kefir (74.52%), cow kefir (73.23%), sheep yogurt (48.56%), camel yogurt (37.51%), cow yogurt (34.42%), and goat yogurt (30.32%). Eventually, the inhibition rate of sheep milk kefir reached 96.50% after 20 days of storage. Conclusion: It seems that kefir may be used as a nutraceutical and functional food against C.albicans infections.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"1 1","pages":"94-100"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84215767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acute Pancreatitis Caused by COVID-19 in a Child: A Case Report","authors":"M. Gholami, R. Arjmand","doi":"10.34172/IJEP.2020.23","DOIUrl":"https://doi.org/10.34172/IJEP.2020.23","url":null,"abstract":"Coronavirus disease 2019 (COVID-19) occurs in children as well as adults and it is mainly associated with symptoms of respiratory and gastrointestinal infections. The patient was a 13-year-old child who had no symptoms of a respiratory infection and complained of abdominal pain and vomiting. A COVID-19 polymerase chain reaction (PCR) test was requested, which was positive. Based on laboratory test results indicating high serum amylase and lipase levels and imaging findings, acute pancreatitis was diagnosed, and after supportive treatment, the patient was discharged in good general condition. During the SARS-CoV-2 pandemic, children who refer to medical centers with gastrointestinal symptoms and pancreatitis should be checked for COVID-19.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"23 1","pages":"107-109"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75319745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Prevalence of ces and cytk Genes of Bacillus cereus Isolated From Raw Milk in Tabriz, Iran","authors":"Mahtab Hamidpour, S. Mahdavi","doi":"10.34172/IJEP.2020.17","DOIUrl":"https://doi.org/10.34172/IJEP.2020.17","url":null,"abstract":"Background: Bacillus cereus is a gram-positive and spore-forming bacterium which is widespread in nature. It also has been known as a major foodborne pathogen that often plays a role in the contamination of ready-to-eat and dairy products. It causes two different types of food poisoning in human: the diarrheal type and the emetic type. Objective: The current study was planned to determine the prevalence of ces and cytk genes of Bacillus cereus isolated from raw milk in Tabriz, Iran. Materials and Methods: In this study, 40 B. cereus strains isolated from cow raw milk, that had already been identified phenotypically, were assessed for molecular confirmation by polymerase chain reaction (PCR) method. Then, they were evaluated for presence of ces and cytK genes by specific primers. Results: Of 40 B. cereus strains, 39 strains were confirmed molecularly. The frequency of cytK and ces genes was reported 38 (97.43%) and 0 (0%), respectively. Conclusion: The results of present study showed that B. cereus strains isolated from raw milk had high potential in causing diarrhea poisoning. Therefore, using procedures to reduce the bacterial contamination during the processing of dairy product is essential.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"2 1","pages":"76-79"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85594268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Akhlaghi, S. H. Emadi Chashmi, A. Jebelli Javan
{"title":"Prevalence and Antibiotic Resistance of Helicobacter pullorum Isolates in Poultry From Semnan Province, Iran","authors":"H. Akhlaghi, S. H. Emadi Chashmi, A. Jebelli Javan","doi":"10.34172/IJEP.2020.22","DOIUrl":"https://doi.org/10.34172/IJEP.2020.22","url":null,"abstract":"Background: Helicobacter pullorum predominantly colonizes the gut of apparently healthy chickens and the livers and intestinal contents of hens with enteritis and vibrionic hepatitis. Objective: The aim of this study was to assess the prevalence and antibiotic resistance of Helicobacter pullorum in broiler chickens, laying hens, and turkeys in Semnan province. Materials and Methods: A total of 300 samples were collected from 60 poultry farms in Semnan province, including 240 cecal samples from 48 broiler farms, 30 fecal samples from 6 laying hen farms, and 30 cecal samples from 6 turkey farms. Each sample was analyzed by conventional culture method and biochemical tests. The suspected colonies were subjected to polymerase chain reaction (PCR) using 16S rRNA gene. Antibiotic resistance of the confirmed colonies was determined using disk diffusion method. Results: Of 300 samples, 85 (28.3%) samples obtained from 36 (60%) poultry farms were positive for H. pullorum. Of these samples, 72 (30%) were from 30 (62.5%) broiler farms, 4 (13.3%) were from 2 (33.3%) laying hen farms, and 9 (30%) were from 4 (66.7%) turkey farms. Moreover, resistance to ciprofloxacin was observed in all of the H. pullorum isolates. Conclusion: This study demonstrated the moderate prevalence of H. pullorum in poultry in Semnan province for the first time, while the prevalence of this pathogen in laying hen and turkey has not been determined in Iran. In addition, this study could reveal the antibiotic resistance profile of H. pullorum as the first report in Iran. Therefore, more studies are needed to focus on the prevalence and antibiotic resistance of H. pullorum in poultry in other regions of Iran.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"1984 1","pages":"101-106"},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82238155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. M. Soltan Dallal, M. Abdi, M. Khalilian, Z. Rajabi, R. Bakhtiari, M. K. Sharifi Yazdi, Somayeh Yaslianifard, Seyedeh Masoumeh Abrishamchian Langroudi
{"title":"Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks","authors":"M. M. Soltan Dallal, M. Abdi, M. Khalilian, Z. Rajabi, R. Bakhtiari, M. K. Sharifi Yazdi, Somayeh Yaslianifard, Seyedeh Masoumeh Abrishamchian Langroudi","doi":"10.34172/IJEP.2020.18","DOIUrl":"https://doi.org/10.34172/IJEP.2020.18","url":null,"abstract":"Background: Foodborne diseases are a major problem worldwide. The epidemiological investigations in many parts of the world have shown an increase in infections caused by Salmonella serovars. Furthermore, the emergence of drug resistance among them has become a major global concern and awareness of the resistance patterns of Salmonella could be very useful in treatment of diseases. Objective: This study aimed to investigate Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA gene and to determine their antimicrobial susceptibility pattern. Materials and Methods: A total of 614 diarrheal stool samples were collected from 173 foodborne outbreaks in different provinces of Iran during one year. Identification of Salmonella was carried out by phenotypic and molecular (16s-23srRNA gene detection) methods and antibiotic susceptibility was performed using disc diffusion method. Results: Out of 614 samples, 18 isolates were identified as Salmonella of which 16 (88.9%) isolates were Salmonella Enteritidis and 2 (11.1%) isolates as Salmonella Paratyphi A. All isolates were sensitive to ceftazidime, and high resistance was seen with nalidixic acid with 14 (77.8%) isolates. Conclusion: Increasing antibiotic resistance in many bacterial pathogens such as Salmonella has been a major threat for human health. Therefore, identifying the antibiotic resistance patterns of Salmonella serovars may help in treatment of the associated infections.","PeriodicalId":31016,"journal":{"name":"International Journal of Enteric Pathogens","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89561700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}