Arquivos do Instituto Biologico最新文献_第7页

Pathogenicity of Metarhizium anisopliae and Beauveria bassiana fungi to Tetranychus ludeni (Acari: Tetranychidae) 绿僵菌和球孢白僵菌对中国叶螨的致病性(蜱螨目:叶螨科)

Arquivos do Instituto Biologico Pub Date : 2019-12-02 DOI: 10.1590/1808-1657000272018

Samuel Luan Pereira, Tatiane Carla Reis, Ivani Teixeira de Oliveira, E. Ferreira, B. M. de Castro e Castro, Marcus Alvarenga Soares, Victor Hugo Vidal Ribeiro

{"title":"Pathogenicity of Metarhizium anisopliae and Beauveria bassiana fungi to Tetranychus ludeni (Acari: Tetranychidae)","authors":"Samuel Luan Pereira, Tatiane Carla Reis, Ivani Teixeira de Oliveira, E. Ferreira, B. M. de Castro e Castro, Marcus Alvarenga Soares, Victor Hugo Vidal Ribeiro","doi":"10.1590/1808-1657000272018","DOIUrl":"https://doi.org/10.1590/1808-1657000272018","url":null,"abstract":"ABSTRACT The use of acaricides is the main control method for Tetranychus ludeni (Acari: Tetranychidae) in horticultural crops. This mite has been recorded causing damage to sweet potato (Ipomoea batatas L.). The use of pathogenic fungi is an alternative to chemical control. The objective was to evaluate the pathogenicity of the fungi Metarhizium anisopliae (Meetch) Sorok, and Beauveria bassiana (Bals.) Vuill. to phytophagous mite T. ludeni in sweet potato plants. Conidial suspensions of M. anisopliae and B. bassiana, at concentrations of 106 and 107 con.mL-1 were applied to sweet potato leaves. After 24 hours, five females of T. ludeni newly emerged were released on the leaves. The bioassay was arranged in a completely randomized design with factorial arrangement 2 × 2 (two species of fungi and two concentrations) plus the control (distilled water), with 10 repetitions per treatment. The evaluation consisted of observing of three biological parameters of the mite: mortality, oviposition, and repellency, after 24, 48, 72, and 96 hours of contact with the fungi. The isolates of M. anisopliae cause high mortality rates of T. ludeni in laboratory. Beauveria bassiana has the potential to suppress future generations of mite, reducing its oviposition rate. Repellency behavior was not observed.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44259243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sodium dodecyl sulfate as a viral inactivator and future perspectives in the control of small ruminant lentiviruses 十二烷基硫酸钠作为病毒灭活剂及其在小反刍动物慢病毒防治中的应用前景

Arquivos do Instituto Biologico Pub Date : 2019-11-28 DOI: 10.1590/1808-1657000752018

A. D. de Sousa, R. R. Pinheiro, J. F. Araújo, Dalva Alana Aragão de Azevedo, Renato Mesquita Peixoto, A. Andrioli, Sabrina Tainah da Cruz Silva Bezerra, M. F. da Silva Teixeira

引用次数: 7

Thermal requirements, life expectancy and fertility tables of Aphis craccivora (Hemiptera: Aphididae) in Vigna unguiculata (Fabales: Fabaceae) under laboratory conditions 实验条件下长爪蚜(半翅目:蚜科)的热需求、寿命和育性表

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000142018

José Edmir Girão Filho, Luiz F. M. Pádua, Gilson Lages Fortes Portela, F. M. D. Sousa, J. D. C. Silva

{"title":"Thermal requirements, life expectancy and fertility tables of Aphis craccivora (Hemiptera: Aphididae) in Vigna unguiculata (Fabales: Fabaceae) under laboratory conditions","authors":"José Edmir Girão Filho, Luiz F. M. Pádua, Gilson Lages Fortes Portela, F. M. D. Sousa, J. D. C. Silva","doi":"10.1590/1808-1657000142018","DOIUrl":"https://doi.org/10.1590/1808-1657000142018","url":null,"abstract":"ABSTRACT: The objective of this research was to determine the thermal requirements and develop life expectancy and fertility tables of Aphis craccivora (L.) in Vigna unguiculata (Walp.). The insects were kept in Petri dishes and fed V. unguiculata leaf discs (cultivar BRS-Tumucumaque) under five constant temperatures (18, 22, 25, 28 and 31ºC). Live and dead insects and stages of development; the onset and duration of the pre-reproductive, reproductive and post-reproductive periods; the number of nymphs per female; and the longevity of the adults were quantified daily. Based on these observations, fertility life expectancy tables were developed, and the thermal requirements of the cowpea black-aphid were determined. Increased temperature influenced all stages of insect development, as well as the pre-reproductive and post-reproductive periods and the total number of nymphs per female. The base temperature for development was 9.13ºC with thermal development constant of 99.0 GD. The highest net reproduction rate (Ro) was at 25ºC. At 28ºC, the greatest capacity to increase in number (rm) was observed, as well as the highest finite rate of increase (λ) and the shortest time to double in number (TD). The thermal range between 22 and 28ºC can be considered most favourable to the development of A. craccivora in V. unguiculata. Temperatures below 22 and above 28ºC affect the fertility and survival of cowpea black-aphids. Increased temperature induces reproduction and reduces the longevity and life expectancy of A. craccivora in cowpeas.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1590/1808-1657000142018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67201255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Influence of working pressure and spray nozzle on the distribution of spray liquid in manual backpack sprayers 工作压力和喷嘴对手动背负式喷雾器喷雾液分布的影响

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000442018

Alcides Marangoni Junior, Marcelo da Costa Ferreira

{"title":"Influence of working pressure and spray nozzle on the distribution of spray liquid in manual backpack sprayers","authors":"Alcides Marangoni Junior, Marcelo da Costa Ferreira","doi":"10.1590/1808-1657000442018","DOIUrl":"https://doi.org/10.1590/1808-1657000442018","url":null,"abstract":"ABSTRACT Manual backpack sprayers are widely used in rural properties in Brazil. However, studies that assess their working characteristics, especially spray tip models and working pressure conditions, are scarce. Thus, the aim of this study was to assess how much the working pressure and spray tips influence the distribution quality of phytosanitary spray solutions in manual backpack sprayers. Four spray nozzles (standard flat-fan Magnojet TP11002, turbo flat-fan TeeJet TT11002, hollow-cone TeeJet TXA8002, and disc-core) were assessed in a patternator table at 1, 2, 3, and 4 bar. Analyses of spray distribution profile were performed by symmetry and the coefficient of variation (CV) analysed by the Tukey’s test (p < 0.05). Spray tip models and working pressure influenced in more than 100% the distribution uniformity values of spray solution and in about 50% the useful range of the phytosanitary treatment. Among the models assessed in this study, the turbo flat-fan spray nozzle presents the best set of characteristics to be indicated for manual backpack sprayers aiming at field phytosanitary treatments.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67201718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens 以含有昆虫病原体的食物为食的蜜蜂工蜂的寿命

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000492017

F. C. Colombo, R. A. Maciel, Gabriela Libardoni, F. Raulino, E. Lozano, F. M. C. Maia, P. F. Freitas, P. Neves, M. Potrich

{"title":"Longevity of Apis mellifera workers fed on a diet incorporating entomopathogens","authors":"F. C. Colombo, R. A. Maciel, Gabriela Libardoni, F. Raulino, E. Lozano, F. M. C. Maia, P. F. Freitas, P. Neves, M. Potrich","doi":"10.1590/1808-1657000492017","DOIUrl":"https://doi.org/10.1590/1808-1657000492017","url":null,"abstract":"ABSTRACT: The present study had the objective of evaluating the longevity of A. mellifera workers fed on a diet incorporating commercial entomopathogens, Beauveria bassiana, and Bacillus thuringiensis. It also aimed at verifying possible morphological alterations in the midgut. To this purpose, the entomopathogens used were B. bassiana (Product A) (5.0 × 1011 viable conidia.kg-1), B. thuringiensis (Product B) (2.5 × 109 viable spores.g-1), and B. thuringiensis (Product C) (1.0 × 109 viable spores.g-1); and two controls: T1: sterilized distilled water, and T2: sterilized distilled water + Tween 80® (0.01%). For the bioassays, 2 mL of each treatment were incorporated into Candy paste. For each treatment, 80 bees were individually in flat bottom glass tubes (2.5 cm Ø) covered with voile, containing a piece of cotton soaked in water and Candy paste. These tubes were stored in a B.O.D (30 ± 2°C, R.H 70% ± 10%, 12 h), and mortality was evaluated every six hours, for 10 days. Soon after verifying mortality, two bees per treatment were selected for the removal of their midgut. Midgut samples were processed using standard methodology for Scanning Electron Microscopy (SEM). It was verified that products A, B, and C reduced the longevity of bees when compared to T1 and T2 controls. In the qualitative analyses carried out using SEM, it was not possible to observe external or internal morphological alterations to midgut tissues. Although products A, B, and C cause a reduction in longevity, their presence was not verified when tissues were analyzed using SEM.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67201881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Does the sequence of addition of herbicide and adjuvants to the spray solution influence sicklepod control? 喷洒溶液中除草剂和佐剂的添加顺序是否影响镰状足的控制?

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000542018

Maria Fernanda Tavares Ramos, Renata Thaysa da Silva Santos, Fabiano Griesang, D. P. Almeida, Marcelo da Costa Ferreira

{"title":"Does the sequence of addition of herbicide and adjuvants to the spray solution influence sicklepod control?","authors":"Maria Fernanda Tavares Ramos, Renata Thaysa da Silva Santos, Fabiano Griesang, D. P. Almeida, Marcelo da Costa Ferreira","doi":"10.1590/1808-1657000542018","DOIUrl":"https://doi.org/10.1590/1808-1657000542018","url":null,"abstract":"ABSTRACT The use of adjuvants associated with herbicides aims at improving the performance of application and the consequent increase in the biological effect of the treatment. However, the sequence of product added to the sprayer tank can influence the phytosanitary spray solution. Thus, this study aimed to evaluate the control of Senna obtusifolia as a function of the sequence of addition of the herbicide aminopyralid + fluroxypyr and adjuvants in the preparation of spray solution. Two herbicide doses (1 and 2 L c.p. ha-1) associated with the adjuvants mineral oil (MO), silicone polyether copolymer (SIL), and a mixture of phosphatidylcholine and propionic acid (LEC), all in the proportion of 0.3% v v-1, with alternate addition to the spray solution to evaluate the effects of the preparation sequence. The spray solution volume considered was 150 L ha-1. Evaluations of spray solution stability were performed from the visual evaluation of homogeneity, electrical conductivity, and pH. The effect of treatment on S. obtusifolia control was measured using a scoring scale and dry matter. Correlation coefficients between the evaluations were also determined. No difference of the preparation sequence of spray solution was observed for stability, pH, and electrical conductivity, but an influence was observed on S. obtusifolia control, without changing dry matter accumulation. The treatment with the adjuvant LEC previously added to the herbicide provided a higher control rate at the highest dose, while the adjuvant SIL had the opposite effect.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67202334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sanitary and physiological quality of ‘purple’ corn (Zea mays L.) seeds submitted to thermotherapy “紫”玉米(Zea mays L.)种子经热处理后的卫生和生理品质

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657001222018

Hugo Vieira, J. V. S. Martins, G. G. Barreto, R. D. S. S. Gomes, E. C. Silva, L. C. Nascimento

{"title":"Sanitary and physiological quality of ‘purple’ corn (Zea mays L.) seeds submitted to thermotherapy","authors":"Hugo Vieira, J. V. S. Martins, G. G. Barreto, R. D. S. S. Gomes, E. C. Silva, L. C. Nascimento","doi":"10.1590/1808-1657001222018","DOIUrl":"https://doi.org/10.1590/1808-1657001222018","url":null,"abstract":"ABSTRACT: The use of seeds with good sanitary and physiological potential ensures the establishment of the crop, with a uniform, vigorous and disease-free seedling stand, which will result in good productivity levels. Seen that, the objective was to evaluate the sanitary and physiological quality of purple corn seeds (Zea mays L.) submitted to thermotherapy. They were subjected to heat treatment with immersion in sterile distilled water (SDW) heated at 50, 60 and 70 °C for 1, 2, and 3 minutes. The control (0 min) correspondens only to immersing the seeds in SDW, which were submitted to sanitary and germination tests in a completely randomized experimental design, in a factorial arrangement 3 × 3 + 1 (exposure time × temperature + additional control). Data were subjected to analysis of variance, and the means were compared with the Scott-Knott test up to 5% of probability, with the aid of the statistical program SISVAR®. Immersing Zea mays seeds in hot water at 50, 60 and 70 °C for 1, 2, and 3 minutes is efficient to prevent the increased incidence of Aspergillus sp., Penicillium sp., and Fusarium sp. The heat treatment at 60 °C for 1 and 2 minutes of immersion reduces the incidence of fungal mycoflora without impairing the physiological quality of seeds.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67203031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Occurrence of anti-Brucella abortus agglutinins in small ruminants in Sergipe Sergipe小反刍动物抗流产布鲁氏菌凝集素的发生

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000212019

H. Rizzo, R. L. F. S. Andrade, H. Menezes, U. Nascimento, Icaro D. Silva, T. K. Jesus, J. S. Carvalho, Lília Márcia Paulin Silva

引用次数: 2

Essential oils of Tahiti lemon and cinnamon bark in control of storage fungi and the physiological and sanitary quality of beans 大溪地柠檬精油和肉桂皮精油对储藏真菌和豆类生理卫生品质的控制

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000172019

R. Valentini, L. Bonome, G. S. Moura, Diogo José Siqueira, Yasmin Tomazi, G. Franzener, H. Bittencourt

{"title":"Essential oils of Tahiti lemon and cinnamon bark in control of storage fungi and the physiological and sanitary quality of beans","authors":"R. Valentini, L. Bonome, G. S. Moura, Diogo José Siqueira, Yasmin Tomazi, G. Franzener, H. Bittencourt","doi":"10.1590/1808-1657000172019","DOIUrl":"https://doi.org/10.1590/1808-1657000172019","url":null,"abstract":"ABSTRACT: With the objective to evaluate the efficiency of essential oils of Citrus latifolia (Tahiti lemon) and Cinnamomum zeylanicum (cinnamon bark) in the control of plant pathogens Penicillium sp. and Aspergillus sp. and the quality of the bean seeds, two experiments were conducted. In the first one, the effect of essential oils of C. latifolia and C. zeylanicum was evaluated in vitro development of the fungi Penicillium sp. and Aspergillus sp. and, in the second one, the influence of essential oils on the physiological and sanitary quality of bean seeds. The variables mycelial growth, conidial germination and sporulation of Penicillium sp. and Aspergillus sp. were measured in the first experiment, while the seed germination test, first count of germination, germination speed index (GSI) and sanity test of bean seeds were measured in the second. The essential oil (EO) of C. zeylanicum was more efficient than C. latifolia in the control of Aspergillus sp. and Penicillium sp., but decreased the physiological quality of the beans seeds. The fungal diversity identified in the seed health test was composed by fungi of the genera Aspergillus, Penicillium, Cladosporium, Fusarium, Chaetomium and Rhizopus. The results indicate the potential of the use of these EOs in the seeds treatment.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67201549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Thermal requirements and age-specific life tables of cowpea aphids in cowpea under natural field conditions 自然田间条件下豇豆蚜虫的热需求和年龄特异性生命表

Arquivos do Instituto Biologico Pub Date : 2019-01-01 DOI: 10.1590/1808-1657000502018

José Edmir Girão Filho, Luiz F. M. Pádua, Gilson Lages Fortes Portela, F. M. D. Sousa

{"title":"Thermal requirements and age-specific life tables of cowpea aphids in cowpea under natural field conditions","authors":"José Edmir Girão Filho, Luiz F. M. Pádua, Gilson Lages Fortes Portela, F. M. D. Sousa","doi":"10.1590/1808-1657000502018","DOIUrl":"https://doi.org/10.1590/1808-1657000502018","url":null,"abstract":"ABSTRACT: Thermal requirements and life tables of insects are important tools in the ecological management of pests. Thus, this study aimed to construct age-specific life tables for Aphis craccivora at different times of the year and, based on their thermal requirements, predict the occurrence of adults under field conditions. To that end, aphids of known age were kept in cages over cowpea plants at different times of the year - November 2016 and March, April, and June 2017 - at the Agricultural Sciences Center of the Federal University of Piauí. Parameters of development, fertility, and mortality were observed daily, allowing us to construct age-specific life tables, as well as an accumulated degree-day model to predict adult occurrence. The time of year affected the first and second stages of development of the cowpea aphid, the nymphal and reproductive periods, longevity, the biological cycle, the number of nymphs produced per female, the daily production of nymphs per female, life expectancy (ex), and survival (Lx). However, the fertility life tables showed significant differences only in the net reproduction rate (R0). The proposed degree-day model reached an accuracy of one day or more than the observed value, with a maximum error of 12.9%. We concluded that the proposed model is adequate to predict the occurrence of adults in the field and that the population parameters of A. craccivora in cowpea are negatively affected during November and positively affected in June.","PeriodicalId":30679,"journal":{"name":"Arquivos do Instituto Biologico","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67201932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1