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Fabrication of DGRC cDNA Microarrays DGRC cDNA芯片的制备
Cgb Technical Report Pub Date : 2006-09-01 DOI: 10.2506/CGBTR-200611
J. Andrews, K. Bogart, Angela Burr, J. Conaty
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引用次数: 5
DGRC-2: Spotted oligonucleotide transcriptome microarrays for the Dros oph ila community DGRC-2:斑点型寡核苷酸转录组微阵列用于果蝇群落
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/CGBTR-200601
L. Cherbas, K. Bogart, Yi Zou, P. Cherbas, J. Andrews
{"title":"DGRC-2: Spotted oligonucleotide transcriptome microarrays for the Dros oph ila community","authors":"L. Cherbas, K. Bogart, Yi Zou, P. Cherbas, J. Andrews","doi":"10.2506/CGBTR-200601","DOIUrl":"https://doi.org/10.2506/CGBTR-200601","url":null,"abstract":"The Drosophila Genomics Resource Center (DGRC) provides the research community with access to genomics resources including microarrays. The first generation transcriptome microarrays fabricated by the DGRC (DGRC-1 arrays) are spotted with amplified DNA fragments. The DNA fragments are amplified from genomic DNA template by polymerase chain reaction using gene-specific primers. The primers were designed against release 1 of the","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133143357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
DGRC Vector Standard Operating Procedure 2.0: Preparation of clones for Whatman FTA MicroCards for Distribution by the DGRC (CloneIDs #2000-13999) DGRC矢量标准操作程序2.0:制备用于DGRC分发的Whatman FTA微卡的克隆(cloneid# 2000-13999)
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/CGBTR-200604
K. Klueg
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引用次数: 0
Processing Clones for Whatman FTA Discs 处理克隆的Whatman FTA光盘
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/CGBTR-200602
E. McKillip, K. Klueg
{"title":"Processing Clones for Whatman FTA Discs","authors":"E. McKillip, K. Klueg","doi":"10.2506/CGBTR-200602","DOIUrl":"https://doi.org/10.2506/CGBTR-200602","url":null,"abstract":"Please note: The TE step is necessary to wash off the chemical that Whatman has placed on the filter – it is this chemical that facilitates the lysis of the bacterial clones and allows the DNA to stick. Failure to do the TE wash step will inhibit transformation. However, it is important to note that if the TE sits on the disc for more than one or two seconds, the DNA will elute off and be lost, resulting in a failed transformation.","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"223 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128855802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Re-Annotation of DGRC-1 Amplicon Microarrays from Drosophila melanogaster Annotation version 3.1 to version 4.1 果蝇DGRC-1扩增子微阵列的重新注释版本3.1到版本4.1
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/CGBTR-200607
J. Costello, J. Andrews
{"title":"Re-Annotation of DGRC-1 Amplicon Microarrays from Drosophila melanogaster Annotation version 3.1 to version 4.1","authors":"J. Costello, J. Andrews","doi":"10.2506/CGBTR-200607","DOIUrl":"https://doi.org/10.2506/CGBTR-200607","url":null,"abstract":"","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123263643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
DGRC Vector Standard Operating Procedure 3.0: Transfer of bacterial cultures in a 96-well format to 96-spot CloneSaver FTA cards DGRC载体标准操作程序3.0:将96孔格式的细菌培养物转移到96点CloneSaver FTA卡上
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/cgbtr-200605
Christiane A. Hassel, K. Klueg
{"title":"DGRC Vector Standard Operating Procedure 3.0: Transfer of bacterial cultures in a 96-well format to 96-spot CloneSaver FTA cards","authors":"Christiane A. Hassel, K. Klueg","doi":"10.2506/cgbtr-200605","DOIUrl":"https://doi.org/10.2506/cgbtr-200605","url":null,"abstract":"","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134373449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amino Allyl Labeling and Hybridization Protocol 氨基烯丙基标记和杂交协议
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/CGBTR-200606
K. Bogart, James C. Costello, B. Eads, Elizabeth A. Bohuski, J. Andrews
{"title":"Amino Allyl Labeling and Hybridization Protocol","authors":"K. Bogart, James C. Costello, B. Eads, Elizabeth A. Bohuski, J. Andrews","doi":"10.2506/CGBTR-200606","DOIUrl":"https://doi.org/10.2506/CGBTR-200606","url":null,"abstract":"The SuperScriptTM Plus Indirect cDNA Labeling System is a highly efficient system for generating fluorescently labeled cDNA for use on micorarrays in gene expression studies. It uses an aminoallyl-modified nucleotide together with other dNTPs in a cDNA synthesis reaction with SuperScriptTM III Reverse Transcriptase. After a purification step to remove unincorporated nucleotides, the amino-fluorescent modified cDNA is coupled with a monoreactive, Nhydroxysuccinimide (NHS)-ester fluorescent dye. A final purification step removes any unreacted dye, and the fluorescently labeled cDNA is ready for hybridization to microarrays.","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122559589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Preparation of vectors (clones 1000-1999) for distribution by the DGRC 准备由DGRC分发的病媒(克隆1000-1999)
Cgb Technical Report Pub Date : 2006-05-01 DOI: 10.2506/cgbtr-200603
K. Klueg
{"title":"Preparation of vectors (clones 1000-1999) for distribution by the DGRC","authors":"K. Klueg","doi":"10.2506/cgbtr-200603","DOIUrl":"https://doi.org/10.2506/cgbtr-200603","url":null,"abstract":"","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127019320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
MicrosatDesign is a pipeline for transforming sequencer trace files into DNA markers MicrosatDesign是将测序器跟踪文件转换为DNA标记的管道
Cgb Technical Report Pub Date : 2005-07-01 DOI: 10.2506/CGBTR-200501
Vasanth R. Singan, J. Colbourne
{"title":"MicrosatDesign is a pipeline for transforming sequencer trace files into DNA markers","authors":"Vasanth R. Singan, J. Colbourne","doi":"10.2506/CGBTR-200501","DOIUrl":"https://doi.org/10.2506/CGBTR-200501","url":null,"abstract":"","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2005-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122376096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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