{"title":"处理克隆的Whatman FTA光盘","authors":"E. McKillip, K. Klueg","doi":"10.2506/CGBTR-200602","DOIUrl":null,"url":null,"abstract":"Please note: The TE step is necessary to wash off the chemical that Whatman has placed on the filter – it is this chemical that facilitates the lysis of the bacterial clones and allows the DNA to stick. Failure to do the TE wash step will inhibit transformation. However, it is important to note that if the TE sits on the disc for more than one or two seconds, the DNA will elute off and be lost, resulting in a failed transformation.","PeriodicalId":306015,"journal":{"name":"Cgb Technical Report","volume":"223 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2006-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Processing Clones for Whatman FTA Discs\",\"authors\":\"E. McKillip, K. Klueg\",\"doi\":\"10.2506/CGBTR-200602\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Please note: The TE step is necessary to wash off the chemical that Whatman has placed on the filter – it is this chemical that facilitates the lysis of the bacterial clones and allows the DNA to stick. Failure to do the TE wash step will inhibit transformation. However, it is important to note that if the TE sits on the disc for more than one or two seconds, the DNA will elute off and be lost, resulting in a failed transformation.\",\"PeriodicalId\":306015,\"journal\":{\"name\":\"Cgb Technical Report\",\"volume\":\"223 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cgb Technical Report\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2506/CGBTR-200602\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cgb Technical Report","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2506/CGBTR-200602","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Please note: The TE step is necessary to wash off the chemical that Whatman has placed on the filter – it is this chemical that facilitates the lysis of the bacterial clones and allows the DNA to stick. Failure to do the TE wash step will inhibit transformation. However, it is important to note that if the TE sits on the disc for more than one or two seconds, the DNA will elute off and be lost, resulting in a failed transformation.
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