Y. Mizuno, T. Hatabu, S. Kawazu, G. Masuda, H. Ohtomo, Mamoru Suzuki, S. Kano
{"title":"Cultivation of Plasmodium falciparum isolates under the Anaeropack® gas condition in a portable thermostatic incubator.","authors":"Y. Mizuno, T. Hatabu, S. Kawazu, G. Masuda, H. Ohtomo, Mamoru Suzuki, S. Kano","doi":"10.2149/TMH1973.28.383","DOIUrl":"https://doi.org/10.2149/TMH1973.28.383","url":null,"abstract":"gen at a low concentration (WHO, 1977). Now, the gas condition can be adjusted automatically in an ordinary CO2 incubator (Waki et al ., 1984) or a computerized mixed-gas incubator (Takagi and Waki, 1987; Trager, 1990). Although an incubation system can be easily maintained in a relatively sophisticated laboratory, it is not always an appropriate technology to transfer to the field. The AnaeroPack(R)_ Malaria Culture System (SUGIYAMA-GEN Co., Ltd., Tokyo) was devised as a portable cultivation system in which the proper conditions for growing P. falciparum parasites can be maintained in a sealed jar. Culture-adapted strains of P. falciparum have been reported to grow nicely in systems that use such gas generators as the AnaeroPack(R)•ECampylo","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129445450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ROLE OF ANOPHELES SUBPICTUS AS A PRIMARY VECTOR OF MALARIA IN AN AREA IN INDIA","authors":"S. Chatterjee, G. Chandra","doi":"10.2149/TMH1973.28.177","DOIUrl":"https://doi.org/10.2149/TMH1973.28.177","url":null,"abstract":"Sibling species A of Anopheles subpictus (fresh water form) has been incriminated and established as a primary vector of malaria for the first time in an area (Tarakeswar, West Bengal) in Indian subcontinent with a natural sporozoite (Plasmodium vivax) rate of 0.32% (2/621). Moderate anthropophilic index (41.0) and high survival rate per gonotrophic cycle (0.58) indicate its efficiency as vector. Coincidence of higher malaria cases and higher density of An. subpictus in the study area is of high relevance. No natural sporozoite infection was detected in other anophelines available like An. vagus, An. annularis and An. barbirostris.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114279982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TAXONOMIC NOTES ON SIMULIIDAE (DIPTERA) FROM THAILAND : DESCRIPTION OF A NEW SPECIES AND NEW DISTRIBUTIONAL RECORDS OF NINE KNOWN SPECIES","authors":"C. Kuvangkadilok, H. Takaoka","doi":"10.2149/TMH1973.28.167","DOIUrl":"https://doi.org/10.2149/TMH1973.28.167","url":null,"abstract":"Simulium (Gomphostilbia) chumpornense sp. nov. is described from reared adults, pupae and mature larvae collected from southern Thailand. This new species is assigned to the varicorne species-group within the subgenus Gomphostilbia by having the adult antennae composed of 2+8 segments in place of usual 2+9 segments. In addition, nine known simuliid species, most of which were described from Peninsular Malaysia, are recorded for the first time from Thailand, bringing the total number of simuliid species in this country to 40.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130652939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Hata, F. Aosai, H. Mun, Mei Chen, Masashi Kobayashi, A. A. Khairul, H. Kubosawa, A. Yano
{"title":"Identification of Toxoplasma gondii Tachyzoites and Bradyzoites by a Quantitative Competitive Polymerase Chain Reaction Method after the Acid Treatment.","authors":"H. Hata, F. Aosai, H. Mun, Mei Chen, Masashi Kobayashi, A. A. Khairul, H. Kubosawa, A. Yano","doi":"10.2149/TMH1973.28.189","DOIUrl":"https://doi.org/10.2149/TMH1973.28.189","url":null,"abstract":"It was indicated that tachyzoites and bradyzoites of Toxoplasma gondii (T. gondii) defined by the susceptibility/resistance to acid treatment could be quantitatively analyzed by a quantitative competitive polymerase chain reaction method targeting SAG1 gene, specific for T. gondii. The tachyzoites were destroyed not only by pepsin-HCl but also by HCl or other acid solutions. The acid-induced destructions occurred under the pH conditions lower than pH 1.8. Tachyzoite DNA of T. gondii was easily destructed by acid treatment. These DNA destructions made it possible to differentiate between tachyzoites and bradyzoites by QC-PCR.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126628947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"TAXONOMIC NOTES ON SIMULIUM GOMBAKENSE (DIPTERA : SIMULIIDAE) FROM PENINSULAR MALAYSIA : DESCRIPTIONS OF MALE AND PUPA, AND SUBGENERIC TRANSFER FROM MOROPS TO GOMPHOSTILBIA","authors":"H. Takaoka","doi":"10.2149/TMH1973.28.111","DOIUrl":"https://doi.org/10.2149/TMH1973.28.111","url":null,"abstract":"The male and pupa of Simulium gombakense Takaoka and Davies, 1995, hitherto known only from pharate pupal and larval specimens collected from Peninsular Malaysia, are described for the first time. This species is characterized by its pupal gill Of much inflated form with six finger-like projections and with eight slender thread-like filaments, similar to those found in some Philippine species of the subgenus Morops, in which this species was previously placed. However, S. gombakense is here transferred to the subgenus Gomphostilbia because many hairs are present on the katepisternum but absent on the pleural membrane in the adult male.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130183971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Immunoblot analysis of antigens from Parastrongylus cantonensis, P. costaricensis and P. malaysiensis using serum antibodies against P. cantonensis.","authors":"P. Eamsobhana, H. Yong, S. Ambu","doi":"10.2149/TMH1973.28.115","DOIUrl":"https://doi.org/10.2149/TMH1973.28.115","url":null,"abstract":"Parastrongylus cantonensis, P. costaricensis and P. malaysiensis adult worm antigens were tested for cross-reactivities in immunoblots using sera from patients with parasitologically confirmed parastrongyliasis. Sera from P. cantonensis-infected patients with a high specificity for the 31-kDa diagnostic P. cantonensis antigen cross-reacted with a corresponding component found in P. costaricensis and P. malaysiensis. The immune sera consistently recognized a prominent immunogenic band with a MW of 31 kDa against the antigens prepared from the 3 Parastrongylus species. Sera obtained from patients infected with Gnathostoma spinigerum and normal healthy control sera did not react with the 31-kDa protein from the 3 species of Parastrongylus. The cross-reactivity observed in the immunoblots performed on serum samples from patients with P. cantonensis infection indicates that the 3 Parastrongylus species share the specific 31-kDa antigen. This protein may be used for the immunodiagnosis of human infection with P. costaricensis and P. malaysiensis.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131874928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Taxonomic notes on Simulium siripoomense from Thailand (Diptera: Simuliidae): descriptions of female and pupa, and change of its subgeneric status from Byssodon to Simulium s. str.","authors":"H. Takaoka, M. Mulla","doi":"10.2149/TMH1973.28.25","DOIUrl":"https://doi.org/10.2149/TMH1973.28.25","url":null,"abstract":"The female and pupa of Simulium siripoomense Takaoka and Saito, 1996, hitherto known only from larval specimens collected from Thailand, are described for the first time. Based upon several diagnostic characters found in the female and pupa, this species, previously placed in the subgenus Byssodon, is transferred to the subgenus Simulium s. str., and is further assigned to the malyschevi-group within this subgenus by the characteristic shape of the female genitalia, the pupal gill with six slender filaments per side, and the fenestrate cocoon. This represents the first record of the malyschevi-group in Thailand.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128977482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE USE OF MALARIA DIAGNOSTIC TEST KITS BY TRAVELERS","authors":"M. Kimura, H. Ohtomo, M. Kumagai, Y. Hiroshige","doi":"10.2149/TMH1973.28.1","DOIUrl":"https://doi.org/10.2149/TMH1973.28.1","url":null,"abstract":"Malaria prophylaxis measures of travelers include, in addition to chemoprophylaxis, stand-by treatment (self treatment) which is indicated in areas of low transmission, for short-term travelers and for those with brief, repeated exposure to malaria. The indication of stand-by treatment has now been expanded in some areas, partly due to an increasing concern about the possible adverse effects of malaria chemoprophylaxis. However, the inappropriate use of stand-by treatment in non-malaria cases may expose persons to a significant drug risk, thus developing simple and reliable self diagnostic tests especially for Plasmodium falciparum malaria has been expected. Recently marketed kits for detecting P. falciparum histidine-rich protein 2 (HRP-2) or pLDH could be good candidates for malaria self diagnosis by travelers. Especially a card-type kit detecting HRP-2 is characterized by its simplicity and reliability, often showing a sensitivity of>95% or even sometimes 100%. Although the sensitivity of those tests could not always be 100%, they could be very useful when performed properly, especially in cases travelers develop malaria-like symptoms in areas remote from reliable medical facilities. However, reports of self diagnosis with those kits performed by travelers showed discouraging results. Recent studies highlighted errors of performing these tests correctly and of interpreting previously reacted strips or cards. Travelers should not become a victim of potentially useful malaria diagnostic tests due to their incorrect use. Nonetheless, we still believe that the use of those kits by travelers should not be abandoned, rather they must have potential for application to travelers' malaria. This goal could be accomplished by improving the contents of the kits' instructions and also by giving full oral explanation to travelers at travel clinics.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124037034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Kobayashi, Yoshiya Sato, H. Toma, I. Shimabukuro, T. Tasaki, M. Takara, Y. Shiroma
{"title":"Epidemiological features of strongyloides infection in Okinawa, Japan: Comparative study with other endemic areas.","authors":"J. Kobayashi, Yoshiya Sato, H. Toma, I. Shimabukuro, T. Tasaki, M. Takara, Y. Shiroma","doi":"10.2149/TMH1973.28.9","DOIUrl":"https://doi.org/10.2149/TMH1973.28.9","url":null,"abstract":"The epidemiological features of Strongyloides stercoralis infection in Okinawa, Japan, were studied by comparing with those in Thailand, Laos and Brazil. The prevalence rates of Strongyloides infection in the present study were 9.6% (133/1, 380) in Okinawa, 47.6% (99/208) in Chiang Mai, Thailand, 23.8% (106/445) in Khammouane, Laos, and 12.0% (32/267) in Maceio, Brazil, respectively. The age inclination in aged subjects and sex dominance in males were significant features of Strongyloides infection in Okinawa, suggesting that new infection from the environment does not occur in present-day Okinawa. The epidemiological feature was considered to provide a favorable field to investigate therapeutic efficacy unaffected by reinfection from environment after treatment. Absence of helminth infection other than Strongyloides was an additional feature in Okinawa. The features may also be convenient to study host response and pathogenicity in the Strongyloides infection unaffected by concurrent infection with other helminths.","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"79 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126730525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Shimao, I. Tada, T. Kurata, Yasuhide Nakamura, Hiroshi Takahashi, Taro Yamamoto, Y. Takayama, Mari Mugitani, Takebumi Furuhata, N. Ishikawa, Naoki Furuta
{"title":"SIMPOSIUM IV “CROSS-FIRE : TROPICAL MEDICINE VS. INTERNATIONAL HEALTH”","authors":"T. Shimao, I. Tada, T. Kurata, Yasuhide Nakamura, Hiroshi Takahashi, Taro Yamamoto, Y. Takayama, Mari Mugitani, Takebumi Furuhata, N. Ishikawa, Naoki Furuta","doi":"10.2149/TMH1973.28.46","DOIUrl":"https://doi.org/10.2149/TMH1973.28.46","url":null,"abstract":"","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129502288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}