Y. Mizuno, T. Hatabu, S. Kawazu, G. Masuda, H. Ohtomo, Mamoru Suzuki, S. Kano
{"title":"Cultivation of Plasmodium falciparum isolates under the Anaeropack® gas condition in a portable thermostatic incubator.","authors":"Y. Mizuno, T. Hatabu, S. Kawazu, G. Masuda, H. Ohtomo, Mamoru Suzuki, S. Kano","doi":"10.2149/TMH1973.28.383","DOIUrl":null,"url":null,"abstract":"gen at a low concentration (WHO, 1977). Now, the gas condition can be adjusted automatically in an ordinary CO2 incubator (Waki et al ., 1984) or a computerized mixed-gas incubator (Takagi and Waki, 1987; Trager, 1990). Although an incubation system can be easily maintained in a relatively sophisticated laboratory, it is not always an appropriate technology to transfer to the field. The AnaeroPack(R)_ Malaria Culture System (SUGIYAMA-GEN Co., Ltd., Tokyo) was devised as a portable cultivation system in which the proper conditions for growing P. falciparum parasites can be maintained in a sealed jar. Culture-adapted strains of P. falciparum have been reported to grow nicely in systems that use such gas generators as the AnaeroPack(R)•ECampylo","PeriodicalId":305785,"journal":{"name":"Japanese Journal of Tropical Medicine and Hygiene","volume":"29 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2000-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Tropical Medicine and Hygiene","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2149/TMH1973.28.383","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
gen at a low concentration (WHO, 1977). Now, the gas condition can be adjusted automatically in an ordinary CO2 incubator (Waki et al ., 1984) or a computerized mixed-gas incubator (Takagi and Waki, 1987; Trager, 1990). Although an incubation system can be easily maintained in a relatively sophisticated laboratory, it is not always an appropriate technology to transfer to the field. The AnaeroPack(R)_ Malaria Culture System (SUGIYAMA-GEN Co., Ltd., Tokyo) was devised as a portable cultivation system in which the proper conditions for growing P. falciparum parasites can be maintained in a sealed jar. Culture-adapted strains of P. falciparum have been reported to grow nicely in systems that use such gas generators as the AnaeroPack(R)•ECampylo
低浓度gen(世卫组织,1977年)。现在,在普通的CO2培养箱(Waki et al ., 1984)或计算机化的混合气体培养箱(Takagi and Waki, 1987;载体,1990)。虽然孵化系统可以很容易地在一个相对复杂的实验室中维持,但它并不总是一种适合转移到现场的技术。AnaeroPack(R)_疟疾培养系统(SUGIYAMA-GEN Co., Ltd, Tokyo)是一种便携式培养系统,可以在密封的瓶子中保持适宜的恶性疟原虫生长条件。据报道,恶性疟原虫的培养适应菌株在使用AnaeroPack(R)•ECampylo等气体发生器的系统中生长良好