H. Hata, F. Aosai, H. Mun, Mei Chen, Masashi Kobayashi, A. A. Khairul, H. Kubosawa, A. Yano
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Identification of Toxoplasma gondii Tachyzoites and Bradyzoites by a Quantitative Competitive Polymerase Chain Reaction Method after the Acid Treatment.
It was indicated that tachyzoites and bradyzoites of Toxoplasma gondii (T. gondii) defined by the susceptibility/resistance to acid treatment could be quantitatively analyzed by a quantitative competitive polymerase chain reaction method targeting SAG1 gene, specific for T. gondii. The tachyzoites were destroyed not only by pepsin-HCl but also by HCl or other acid solutions. The acid-induced destructions occurred under the pH conditions lower than pH 1.8. Tachyzoite DNA of T. gondii was easily destructed by acid treatment. These DNA destructions made it possible to differentiate between tachyzoites and bradyzoites by QC-PCR.
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