M. Živojinović, I. Dobrosavljević, Z. Kulišić, S. Radojicic, T. Bošković, B. Plavšić
{"title":"Trichinellosis in Serbia and possibilities to improve control measures","authors":"M. Živojinović, I. Dobrosavljević, Z. Kulišić, S. Radojicic, T. Bošković, B. Plavšić","doi":"10.2298/vetgl190412018z","DOIUrl":"https://doi.org/10.2298/vetgl190412018z","url":null,"abstract":"Background. Trichinellosis is zoonotic disease caused by parasites of the genus Trichinella, which have a cosmopolitan distribution. In the Balkan region, including Serbia, trichinellosis is endemic. Although trichinellosis shows a decreasing incidence in Serbia, this disease remains a serious problem for human health and animal husbandry. Scope and Approach. To prevent and control Trichinella spp. infections in domestic and sylvatic animals, risk analysis for these zoonotic parasites should be conducted. In Serbia, a limited number of Trichinella have been identified to the species level so far. An institutional, multi-sectoral surveillance system, supported by an adequate legal framework for the detection, surveillance, prevention, control and reporting of this infection in animals and humans, harmonised with the EU legislation, is a priority. Key Findings and Conclusions. One of the main goals of the Serbian veterinary and public health services should be progressive improvement of animal rearing practices, food safety, and hunters? and consumers? education through the ?One-health? approach.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47414446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Serological diagnosis of trichinellosis in patients in Southern and Eastern Serbia in the period from 2007 to 2018","authors":"N. Miladinović-Tasić, A. Ignjatović","doi":"10.2298/vetgl190420013m","DOIUrl":"https://doi.org/10.2298/vetgl190420013m","url":null,"abstract":"Introduction. The Nisava District has had low prevalence and incidence rates of trichinellosis for a long period. The aims of this study were to present the frequency of occurrence of trichinellosis in Nisava District and other districts in Southern and Eastern Serbia during 2007-2018, to investigate the demographic characteristics in the region and to compare the tests used for serological diagnosis of trichinellosis. Materials and Methods. This was a retrospective epidemiological study in which we analyzed the results of serological analyses of 139 serum samples taken from individual patients suspected to be infected with Trichinella spp. Indirect immunofluorescence (IIF) and immunoenzyme (ELISA) methods were used for serological diagnosis of trichinellosis. Results and Conclusions. Of 139 examined sera, 36 (25.9%) were seropositive. Most seropositive findings were encountered in 2012 and 2014 (in both these years, 12 sera were positive, i.e. 33.3% of the total 36 positive sera), when three minor outbreaks of trichinellosis were reported. Seropositivity was equally distributed according to patient sex and according to the district where they reported with illness. Positive serological findings were statistically significantly more common in hospitalized patients than in out-patients. Comparison of the findings with both IIF and ELISA test established the performance level of the tests, with the ELISA test being less sensitive than the IIF test. Immunodiagnostic procedures for the diagnosis of trichinellosis must be improved in view of the sporadic cases and minor outbreaks of the disease in the studied districts of Eastern and Southeastern Serbia.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44075876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Mitrović, B. Milošević, A. Urošević, N. Nikolic, Z. Dakić, I. Nikolic, M. Korac
{"title":"Severe trichinellosis with neurological involvement - neurotrichinellosis: A case report","authors":"N. Mitrović, B. Milošević, A. Urošević, N. Nikolic, Z. Dakić, I. Nikolic, M. Korac","doi":"10.2298/vetgl180311015m","DOIUrl":"https://doi.org/10.2298/vetgl180311015m","url":null,"abstract":"Trichinellosis is the most common and most important parasitic zoonosis in Serbia. Complications of this disease are frequent, and a fatal outcome is possible. A particularly significant complication is the involvement of the central nervous system ? neurotrichinellosis. This case report presents a previously healthy, 51-year-old female from Belgrade, Serbia, a teacher by profession. The disease started with symptoms of gastroenteritis in April, after which symptoms and signs characteristic of trichinellosis occurred (myalgia, fever, bilateral eyelid edema, conjunctivitis). About two weeks before the onset of the first symptoms, the patient consumed pork sausages that had been purchased at a green market. Seven days after the disease started, she was hospitalized at the Clinic for Infectious and Tropical Diseases in Belgrade and the diagnosis of trichinellosis was established. Two days after admission, neurological symptoms appeared ? confusion, apathy, right side weakness of the mimic and limb muscles. The medical imaging techniques (CT scan, MRI) showed multiple hypodense changes in different parts of the brain, which corresponded to vasculitis due to parasitic infection. She was treated with approved antiparasitic and corticosteroid therapy. Despite adequate treatment, neurological sequelae were maintained in the follow-up period, and control imaging techniques revealed residual changes in the brain. This case emphasizes the importance of the strict application of all foreseen preventive measures in the control of trichinellosis, and primarily, mandatory trichinoscopic examination of all meat intended for human consumption.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46898254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mirilovic, Z. Kulišić, B. Vejnović, S. Djuric, B. Plavšić, N. Fejzic, V. Teodorović
{"title":"Economic evaluation of a program for monitoring and controlling Trichinella in pigs","authors":"M. Mirilovic, Z. Kulišić, B. Vejnović, S. Djuric, B. Plavšić, N. Fejzic, V. Teodorović","doi":"10.2298/vetgl190604023m","DOIUrl":"https://doi.org/10.2298/vetgl190604023m","url":null,"abstract":"Introduction. Trichinellosis is a cosmopolitan anthropozoonosis, and is a\u0000 group of systemic diseases caused by larval forms of Trichinella. This is\u0000 one of the oldest and most controversial parasitic zoonoses that has been in\u0000 the forefront of veterinary, medical and biological research for many years.\u0000 Parasites from the genus Trichinella were diagnosed in more than one hundred\u0000 species of domestic and wild mammals and birds. The aim of this study was\u0000 economic evaluation of a program for monitoring and controlling Trichinella\u0000 in pigs. Materials and Methods. A systematic pest rodent control program\u0000 deemed suitable for monitoring and controlling Trichinella in pigs was\u0000 implemented in a representative test zone. The results of the pest rodent\u0000 control program were analyzed using cost-benefit analysis to determine if a\u0000 similar program would be suitable for a larger land area. Results and\u0000 Conclusions. Prior to pest control, the incidence of Trichinella in pigs\u0000 living in the test zone increased annually, while after systematic pest\u0000 rodent control, the incidence of Trichinella-positive pigs decreased. These\u0000 results, as well as relevant economic indicators, enabled us to design two\u0000 mathematical models describing the control and monitoring of Trichinella in\u0000 a larger land area. In this area, Srem Region, Serbia, a suitable\u0000 Trichinella control program in pigs is clearly justified because the profit\u0000 to cost ratio was positive for both models.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41804236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Vasilev, N. Ilić, Ljiljana Sofronić-Milosavljević
{"title":"First Serbian external quality assessment to detect Trichinella larvae in meat by the Magnetic Stirrer Method","authors":"S. Vasilev, N. Ilić, Ljiljana Sofronić-Milosavljević","doi":"10.2298/VETGL181220006V","DOIUrl":"https://doi.org/10.2298/VETGL181220006V","url":null,"abstract":"Introduction. Inter-laboratory comparative testing must be conducted under the direction of the National Reference Laboratory for Trichinella in each Member State of the EU with the aim to ensure the quality of the approved digestion method and to evaluate the competence of laboratories in Trichinella detection. For the first time in Serbia, the National Reference Laboratory for Trichinellosis (NRLT INEP) organized such external quality assessment (EQA) for the detection of Trichinella larvae in meat by the Magnetic Stirrer Method (MSM). The aim of this study was to enable laboratories interested to take part in the EQA and to assess the quality of their MSM performance. Materials and Methods. The EQA was organized by NRLT INEP according to ISO/IEC 17043:2010. The EQA test panel, which was sent to eight laboratories within Veterinary Institutes, consisted of three 100?5 g minced pork meat balls with identical numbers of larvae. Two meat balls were spiked with five viable Trichinella spiralis muscle larvae (L1), and one meat ball was not spiked. Results and Conclusions. Evaluation made on the basis of qualitative results showed that 100% of participants successfully completed the testing. Quantitative evaluation showed that, on average, only 60% of Trichinella larvae were detected among all participants. The main importance of this study is that it enabled laboratories across the country to be compared. This should be efficient stimulus for improvement of analytical performance over time. Therefore, it will be of value if the participants and other official laboratories in Serbia take part annually in EQAs organized at national level. The results obtained in this study could provide useful information to the Ministry of Agriculture, Forestry and Water Management, Veterinary Directorate and be of importance for promoting the one health concept in the country as a whole.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45879078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Important biological characteristics of Trichinella genotypes","authors":"J. Petrović","doi":"10.2298/vetgl190808021p","DOIUrl":"https://doi.org/10.2298/vetgl190808021p","url":null,"abstract":"Background. To control Trichinella, one of the most important food borne parasites, it is necessary to know the biology of the parasite. Scope and Approach. The pathogenicity of Trichinella genotypes mainly depends on its biological characteristics: reproductive capacity index (RCI), infectivity towards different animal species and humans, differences in the number of newborn larvae (NBL) and susceptibility to environmental conditions. Key Findings and Conclusions. Among the Trichinella, T. spiralis has the highest RCI for domestic, synanthropic and some wild animals. This genotype is the most infectious for domestic and wild pigs and has the highest NBL index. T. spiralis is more susceptible to high temperatures, freezing and decay of dead host tissues than the sylvatic genotypes. The pathogenicity of T. spiralis results in its easy and rapid spread across populations of domestic pigs, thus creating opportunities for human disease. T. britovi has a moderate NBL and low RCI for pigs, mice and foxes, but this genotype is resistant to freezing. Other genotypes have high to moderate RCI, NBL and infectivity towards wild animals, have good tolerance towards adverse environmental conditions of low temperature and high humidity, and they usually spread in wild animal populations.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44796519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Jankovic, V. Drašković, Š. Pintarič, M. Mirilovic, S. Djuric, Nada Tajdić, R. Teodorović
{"title":"Rodent pest control","authors":"L. Jankovic, V. Drašković, Š. Pintarič, M. Mirilovic, S. Djuric, Nada Tajdić, R. Teodorović","doi":"10.2298/vetgl190507020j","DOIUrl":"https://doi.org/10.2298/vetgl190507020j","url":null,"abstract":"Background. Rodent pests are natural reservoirs and vectors of a vast array of human and animal diseases caused by bacteria, rickettsia, viruses, protozoans, fungi and some parasites. The most important risk factor for human infection with Trichinella is the rearing of pigs on small farms and by rural households, if rodent pest control is not conducted regularly. Rodent pests cause economic losses by consuming, contaminating and/or damaging foods intended for human or animal consumption. Scope and Approach. The aim of this work is to point out the epidemiological and epizootiological importance of regular rodent control, and the importance of integrated use of all measures in deratization, as well as mistakes which can be made in implementing this procedure. Key Findings and Conclusions. The control of populations of pest synanthropic and hemisynanthropic rodents is a very complex and delicate task to carry out. Given all characteristics of the majority of rodent pest species, e.g. high reproductive potential, extraordinary adaptation to life in a variety of habitats and ability to develop resistance to anticoagulant rodenticides, the issue of controlling the number of rodent pests is considerably more problematic than it might seem at first glance. Therefore, appropriate scientific and professional knowledge is necessary to accomplish effective rodent control, which if done improperly, can have far-reaching negative consequences for human populations, non-target species and workers performing the task.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46622553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trichinella and trichinellosis in Europe","authors":"E. Pozio","doi":"10.2298/vetgl190411017p","DOIUrl":"https://doi.org/10.2298/vetgl190411017p","url":null,"abstract":"Background: Trichinellosis, the proper term for the human zoonotic disease is caused by nematodes of the genus Trichinella. These zoonotic parasites show a cosmopolitan distribution in all the continents but Antarctica. They circulate in nature by synanthropic-domestic and sylvatic cycles that are correlated with each other. Today, nine species and three genotypes are recognized in this genus, all of which infect mammals, including humans, while one species also infects birds, and two other species also infect reptiles. Scope and Approach: To review the recent literature on these pathogens, which are unusual among the other nematodes in that the worm undergoes a complete developmental cycle, from larva to adult to larva, in the body of a single host, which has a profound influence on the epidemiology of trichinellosis as a zoonosis. When the cycle is complete, the muscles of the infected animal contain a reservoir of larvae capable of long-term survival. Humans and other hosts become infected by ingesting muscle tissues containing viable larvae. Key Findings and Conclusions: The main source of human infection is raw or under-cooked meat products from pig, wild boar, bear, walrus, and horses, but meat products from other animals have been implicated. Both pre-slaughter prevention and post-slaughter control can be used to prevent Trichinella infections in animals. The first involves pig management control in high containment level farms as well as continuous surveillance programs. Meat inspection is a successful post-slaughter strategy. However, continuous consumer education is of great importance in countries where meat inspection is not mandatory.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47341090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Prodanović, Sreten Nedić, O. Radanovic, V. Milićević, I. Vujanac, J. Bojkovski, B. Kureljušić, Sveta Arsić, Ljubomir Jovanović, D. Kirovski
{"title":"Occurrence of neonatal diarrhea in calves with iron-deficiency anemia","authors":"R. Prodanović, Sreten Nedić, O. Radanovic, V. Milićević, I. Vujanac, J. Bojkovski, B. Kureljušić, Sveta Arsić, Ljubomir Jovanović, D. Kirovski","doi":"10.2298/VETGL181210011P","DOIUrl":"https://doi.org/10.2298/VETGL181210011P","url":null,"abstract":"Introduction. Neonatal calves are often deficient in iron. Accumulating\u0000 evidence indicates that iron status is associated with disease pathologies\u0000 including diarrhea. Our objective was to examine the association between iron\u0000 status and gut function in neonatal calves with and without a history of calf\u0000 diarrhea. Materials and Methods. Calves were divided into two groups based on\u0000 their history of diarrhea; the first group were diarrheic calves (n=6) and\u0000 the second group were non-diarrheic healthy calves (n=6). Blood samples\u0000 (n=12) were collected at day 12 of age and erythrogram determination and\u0000 measurements of serum iron and total iron binding capacity were performed.\u0000 Hematological values were measured using an automatic analyzer, and\u0000 biochemical properties were determined spectrophotometrically. Fecal samples\u0000 were obtained from all calves and pH measured using semi quantitative test\u0000 strips as well as being examined by bacterial cultivation for enterotoxigenic\u0000 Escherichia coli, Salmonella spp. and Clostridium perfringens, by RT-PCR for\u0000 the presence of bovine rotavirus, bovine coronavirus and bovine viral\u0000 diarrhea virus, and by microscopy for the presence of Cryptosporidium parvum.\u0000 Results and Conclusions. There were significant iron-related changes for most\u0000 hematological indices in diarrheic calves; and iron (Fe) deficiency and\u0000 microcytic, hypochromic anemia were diagnosed. The pH of the feces was\u0000 significantly higher in diarrheic calves than in the non-diarrheic healthy\u0000 group (P<0.01). All fecal samples were negative for the analyzed enteric\u0000 pathogens. According to the results obtained, calves experiencing iron\u0000 deficiency anemia exhibit changes in gut function leading to diarrhea as\u0000 compared with a matched group of healthy calves.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47639148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Čobanović, U. Jamnikar-Ciglenečki, A. Kirbiš, M. Križman, M. Stukelj, N. Karabasil
{"title":"Impact of various housing conditions on the occurrence of pathological lesions in slaughtered pigs","authors":"N. Čobanović, U. Jamnikar-Ciglenečki, A. Kirbiš, M. Križman, M. Stukelj, N. Karabasil","doi":"10.2298/VETGL190318010C","DOIUrl":"https://doi.org/10.2298/VETGL190318010C","url":null,"abstract":"Introduction. This study assessed the effect of farming system, feeding system, floor type, housing density and gender on the occurrence of pathological lesions in slaughtered pigs. Materials and Methods. The study was conducted on 400 pigs from four farms with different housing conditions. The plucks of 100 slaughtered pigs from each farm were examined for pneumonia, pleurisy, pericarditis and milk spots. Results and Conclusions. Pigs housed in a large-scale indoor farm had the lowest incidence of pneumonia. The highest occurrence of pneumonia was recorded in pigs fattened in a small-scale farm in pens with outdoor access. Pigs reared on farms in pens with outdoor access had the highest incidence of milk spots. Pigs from farms using pellet feeding systems had higher incidences of pneumonia, pleurisy and milk spots than those from a farm using a liquid feeding system. A rearing system comprising concrete floors without bedding resulted in the highest incidence of pneumonia in pigs. Pigs from a farm using a concrete floor with bedding had the highest occurrence of milk spots. The lowest incidence of milk spots was recorded in pigs from farms using fully-slatted floors. Compared to low housing density, high housing density resulted in higher incidences of pneumonia, pericarditis and milk spots in pigs. A higher prevalence of lung lesions was detected in barrows than in gilts. This study showed the most significant risk factors for the occurrence of pathological lesions in slaughtered pigs are pens with outdoor access, pellet feeding system, concrete floor, high housing density and gender.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42096347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}