Veterinarski Glasnik最新文献

{"title":"Platelet-poor plasma of athletes is a potent inducer of myogenic differentiation of C2C12 myoblasts","authors":"I. Maslovarić, V. Ilić, A. Stancic, J. Santibañez, D. Trivanović, Ivana T. Drvenica, J. Krstić, S. Mojsilović, I. Okic-Djordjevic, D. Bugarski","doi":"10.2298/vetgl190414019m","DOIUrl":"https://doi.org/10.2298/vetgl190414019m","url":null,"abstract":"Introduction. Blood products, i.e. platelet rich plasma (PRP), leukocyte-poor plasma (PRP) and platelet poor plasma (PPP), have previously been used to improve muscle regeneration. In this study, six months? frozen-stored PPP of individuals who practiced different types of physical exercise was analysed; it could steer mouse C2C12 myoblast cells towards proliferation, migration and myogenic differentiation, and it could affect the morphology/shape of myotubes. Materials and Methods. PPP of male Olympic weightlifters, football players and professional folk dancers, aged 15-19, was collected 12 h post-training and stored for 6 months at -20?C. C2C12 cell proliferation was assessed by MTT test, motility by scratch assay, myogenic differentiation by myotube formation and gelatinase activity by gel-zymography. Results and Conclusions. PPP induced proliferation and migration of C2C12 cells. Proliferative capacity was as follows: weightlifters > dancers > football players; mean migratory capacity was: weightlifters = dancers > football players. PPP induced formation of myotubes; significant inter-individual variations were detected: PPP from weightlifters induced formation of round myotubes, and PPP from football players and dancers induced formation of elongated myotubes. The mean myotube area was as follows: football players > dancers > weightlifters. PPP gelatinolytic activity was observed; it was negatively correlated with C2C12 myoblast proliferation. These results provide general but distinct evidence that PPP of individuals practicing certain types of exercise can specifically modify myoblast morphology/function. This is significant for explaining physiological responses and adaptations to exercise. In conclusion, long-term, frozen-stored PPP preserves its potential to modify myoblast morphology and function.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46261893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the microbiological quality of feed using the Verbands Deutscher Landwirdschaftlicher Untersuchungs und Forschungsanstalten (VDLUFA) method","authors":"Ksenija Nesic, M. Pavlovic, Snezana Ivanovic","doi":"10.2298/vetgl190710016n","DOIUrl":"https://doi.org/10.2298/vetgl190710016n","url":null,"abstract":"Introduction. The Verbands Deutscher Landwirdschaftlicher Untersuchungs und Forschungsanstalten (VDLUFA) is an association of German agronomic, analytical and scientific institutes dealing with various types of research in the field of agronomy and the development, adaptation and validation of analytical methods. This association has established an approach for determining the microbiological quality of feed based on the contents of molds, yeasts and bacteria by taking into account not only their total numbers (cfu/g), but also their potential pathogenicity. According to the degree of the potential pathogenicity, microorganisms are divided into seven groups. Based on the number of microorganisms in the seven groups, feed and feedingstuffs are classified into four categories according to microbiological quality. Materials and Methods. This method for categorization of feed is described in Methods Book III. Detailed instruction is given within the four Standard Operating Procedures (SOPs). Results and Conclusions. The VDLUFA method of assessing animal feed and feedingstuffs is a far more detailed approach that gives a more complete picture of the actual microbiological status of feed than the approach currently used in Serbia. Feed placed on the market to be used in animal nutrition should meet the criteria for classification into classes I to III, in accordance with the parameters laid down in the method. Animal feed that belongs to class IV is not suitable for animal feeding. This method could be a useful guideline in the modernization of hygienic standards for feed in Serbia in the future.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48503581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Entamoeba sp. infection in a bearded dragon (Pogona vitticeps)","authors":"D. Schwartz, I. Ali, Shantanu Roy, L. Pohlman, Brandy C Kastl, D. Eshar","doi":"10.2298/vetgl190919007s","DOIUrl":"https://doi.org/10.2298/vetgl190919007s","url":null,"abstract":"A 3-year-old, male intact, pet inland bearded dragon (Pogona vitticeps) presented with a history of diarrhea, progressive inappetence and weight loss. A palpable cranial celomic mass was identified on physical examination and confirmed to be hepatic in origin by celomic ultrasonography. Hematologic and biochemical abnormalities were mild and consistent with inflammation, regenerative anemia, and hepatocellular injury. Fine needle aspiration of the liver masses was suggestive of amoebiasis and the patient was humanely euthanized. PCR and Sanger DNA sequencing of liver aspirates were supportive of Entamoeba infection, although definitive speciation was not possible. Pathogenic amoebiasis due to infection by E. invadens has been reported in a wide range of reptiles and is an important cause of morbidity and mortality in these species.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42165416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of the most significant coronavirus infections in veterinary medicine","authors":"J. Nišavić, N. Milic, A. Radalj","doi":"10.2298/vetgl2001001n","DOIUrl":"https://doi.org/10.2298/vetgl2001001n","url":null,"abstract":"Background. Coronaviruses (CoVs) have been recognized in veterinary virology\u0000 for a long time and comprise a large group of RNA viruses responsible for\u0000 enteric, respiratory, hepatic, and neurologic diseases in a variety of\u0000 animal species and humans. These viruses are very adaptable considering\u0000 their highly error-prone replication process and recombination ability,\u0000 resulting in remarkable mutability and efficient expansion of their host\u0000 range and tissue tropism. Scope and Approach. In the recent past, after the\u0000 outbreaks caused by SARS-CoV in 2002 and MERS-CoV in 2012, CoVs became a\u0000 research focus in the scientific community. Moreover, the ongoing SARS-CoV-2\u0000 pandemic raised more questions concerning the threats posed by these\u0000 viruses. Several significant examples of coronaviruses jumping the species\u0000 barrier and changing their tropism have been reported in the past, and novel\u0000 viruses of both animals and humans have appeared as a consequence. This\u0000 paper reviews some of the examples of CoV mutability and the most notable\u0000 animal coronaviruses of veterinary relevance. Key Findings and Conclusions.\u0000 There is still no proof that the novel virus SARS-CoV-2 can be transmitted\u0000 to humans from domestic animals, and its recent cross-species jump is\u0000 currently being intensively researched. Intensified and diverse human\u0000 activities that lead to the disruption of ecosystems contribute to the\u0000 increased risk of contact with animals that might represent virus\u0000 reservoirs. The need for constant surveillance of CoVs and expanded studies\u0000 of their virological traits, mutation mechanisms, diversity, prophylactic\u0000 and therapeutic measures highlight the key role of both veterinarians and\u0000 medical doctors in order to preserve the health of the human population.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49110239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preliminary evaluation of complete blood count and diurnal variation of hematological parameters in black rat snake (Pantherophis obsoletus)","authors":"M. Belic, M. Lukač, Nika Dvojković, Tena Galešić, M. Robić, R. Turk","doi":"10.2298/vetgl190506004b","DOIUrl":"https://doi.org/10.2298/vetgl190506004b","url":null,"abstract":"Introduction. The purposes of this study were to determine the presence of daily variations in hematological parameters of the black rat snake (Pantherophis obsoletus) and to compare them with the reference interval values of its subspecies, yellow rat snake (Pantherophis obsoleta quadrivittata). Materials and Methods. Blood from four black rat snakes was collected in the morning and in the evening, and after each collection, blood smears, packed cell volume (PCV), red blood cell count (RBC) and hemoglobin concentration (HGB) were determined, while erythrocyte indices of mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated and differential leukocyte counts were evaluated. Results and Conclusions. There were no statistically significant differences in any of the blood parameters in the morning and in the evening. Most of the complete blood count values in the black rat snakes were lower, but still within the reference range of those found in the literature for the yellow rat snake. The results of hematological parameters of the black rat snake could be beneficial for further research of physiological and pathological variations in complete blood count of these snakes, for diagnosing health conditions and for detecting various diseases.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49439586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Congenital thoracic kyphosis caused by hemivertebra in a pug puppy","authors":"D. Davitkov, D. Marinković, Darko Davitkov, V. Nesic","doi":"10.2298/VETGL190115007D","DOIUrl":"https://doi.org/10.2298/VETGL190115007D","url":null,"abstract":"Congenital spine deformation caused by vertebral malformation is reported in different brachycephalic dog breeds, but also in other large dog breeds. The most severe clinical signs are due to spinal cord compression. Depending on localisation of the lesion, clinical signs can manifest differently. A five-month-old pug was brought for autopsy to the Department of Forensic Veterinary Medicine, Faculty of Veterinary Medicine, University of Belgrade. The first clinical signs, including general weakness and difficulty to jump appeared when he was three and a half months old. They became more severe until the dog was four months old. Radiography and myelography showed severe kyphosis and spinal cord compression in the T8-T9 region. After euthanasia, an autopsy was performed. Pathomorphological examination showed severe deformation of the spine, kyphosis and a wedge-shaped T8 vertebra. Although, literature data indicates that clinical signs can be latent until five or six months of age, this case shows that even in young puppies, three to four months old, serious clinical signs can be present. This is of great clinical importance, not just for veterinarians, but also for brachycephalic dog owners and breeders.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42342486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First report of autochthonous canine angiostrongylosis in Bosnia and Herzegovina","authors":"O. Stevanović, M. Dobrijević, Dejan Vujanić, D. Nedic, T. Ilić, Igor Trbojević","doi":"10.2298/vetgl2001085s","DOIUrl":"https://doi.org/10.2298/vetgl2001085s","url":null,"abstract":"This report describes a case of autochthonous canine angiostrongylosis in a\u0000 mixed-breed dog in Bosnia and Herzegovina. The owner not?d the dog had lost\u0000 appetite and weight, accompanied by a non-productive and intermittent cough.\u0000 Physical examination revealed increased bronchovesicular pulmonary sounds\u0000 and a caudally expanded lung field. Haematological parameters suggested mild\u0000 dehydration in the dog. Radiological examination of the dog?s thorax showed\u0000 a mild bronchointerstitial pattern with alveolar patches in the cardiac\u0000 lobes. Coprological examination revealed the first stage (L1)\u0000 Angiostrongylus vasorum larvae (Baillet, 1866). The dog was treated with two\u0000 doses of 0.4 mg/kg of ivermectin administered subcutaneously with a time\u0000 interval of 21 days between the two applications. Five weeks after the\u0000 initial diagnosis, a repeated coprological examination revealed no evidence\u0000 of L1 A. vasorum larvae in the dog?s faeces. This case report confirms the\u0000 presence of A. vasorum in a dog from Bosnia and Herzegovina.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42840552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare case in a dog of intraorbital foreign body with late signs of cornea penetration - presenting initially as anterior uveitis","authors":"M. Hadži-Milić, B. Prokic, P. Milosavljević, B. Prokić, J. Vasic, S. Pajić, Nikola Radmanović, Ivana Hadži-Milić","doi":"10.2298/vetgl191003022h","DOIUrl":"https://doi.org/10.2298/vetgl191003022h","url":null,"abstract":"An unusual case is presented of an unsuspected orbital metallic foreign body (sewing needle) in a dog. The skin entrance wound injury was undetected during two clinical examinations, likely due to the quick skin healing and punctiform nature of the wound in the skin/muscles/orbit. The most unusual characteristic of this case is the cause of the wound. The metallic foreign body could have penetrated the cornea through an oblique limbal/scleral pathway at the moment of injury, leaving no obvious signs of corneal injury in the first clinical examination. Secondly, the corneal injury perhaps occurred due to the dog rubbing its head on the floor between the initial examination and the first follow-up examination after seven days. At first, the case presented as anterior uveitis with an unknown cause. Due to quick scar formation of wounds in dogs and the fact that punctiform wounds can be difficult to detect, an orbital foreign body was not initially suspected. However, seven days afterwards, a new sign of triangular limbus-based corneal opacification appeared. This led to suspicion of a previous corneal injury and the nearby (orbital) presence of a foreign body. This diagnosis was confirmed by radiography and the intraorbital sewing needle was extracted by surgery. Lack of sophisticated techniques did not prevent an adequate diagnosis and an excellent surgical outcome.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48943274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Trichinella infections in pigs and Trichinellosis in humans in Serbia from 1994 to 2018","authors":"M. Mirilovic, Nada Tajdić, B. Vejnović, S. Djuric, Nikola Mirilovic, Slavica Maris, V. Šaponjić","doi":"10.2298/vetgl191022024m","DOIUrl":"https://doi.org/10.2298/vetgl191022024m","url":null,"abstract":"Introduction. Trichinellosis is a disease in humans caused by parasites of\u0000 the genus Trichinella, and these roundworms can occur in a variety of\u0000 animals (over one hundred mammal species). Members of the genus Trichinella\u0000 are present in almost all continents and in all climate zones. Intensive\u0000 studies on the eradication of this disease have been going on for a long\u0000 period, but despite the finances invested in research projects,\u0000 trichinellosis is still present in the 21st century and poses a major health\u0000 issue all over the world. According to current scientific estimations, there\u0000 are over 27 million Trichinellainfected people in the world. The aim of our\u0000 study was to determine the distribution and trends for Trichinella infection\u0000 in pigs and trichinellosis in humans in Serbia between 1994 and 2018.\u0000 Materials and Methods. Data for the 25-year surveillance period of\u0000 Trichinella cases registered in pigs and humans in Serbia was gathered from\u0000 the Veterinary Directorate and from the Institute of Public Health of the\u0000 Republic of Serbia. The data obtained was analysed with the relative numbers\u0000 of structure and dynamics, indices and descriptive statistical indicators.\u0000 Results and Conclusions. During the research period, 14,837 pigs were\u0000 diagnosed as infected with Trichinella. Out of this number, 87.31% of pigs\u0000 were identified in the five epizootiological regions, and only 12.69% were\u0000 diagnosed in the non-epizootiological regions in Serbia. During the period\u0000 1994-2018 in Serbia, a total of 6,850 people were treated for Trichinella\u0000 infection. Out of this number, 4,153 (60.63%) people were from the five\u0000 epizootiological regions. The trend-line describing the presence of\u0000 Trichinella in pigs was defined by a fourth degree polynomial function.\u0000 Meanwhile, the trend-line describing the presence of trichinellosis in\u0000 humans was defined by a sixth degree polynomial function. Trichinellosis in\u0000 Serbia is most common during the winter season, from December to March.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45502976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological screening of patients with clinical suspicion of trichinellosis in Belgrade from 2009 to 2018","authors":"Z. Dakić, N. Mitrović, S. Jovanović, B. Milošević, I. Milošević, G. Stevanović, N. Nikolic, Aleksandar Marković, A. Urošević, J. Malinić, Biljana Bojić, M. Korac","doi":"10.2298/vetgl190311014d","DOIUrl":"https://doi.org/10.2298/vetgl190311014d","url":null,"abstract":"Introduction. Trichinellosis is one of the most important foodborne diseases in Serbia. Most patients with suspected trichinellosis in Belgrade are referred to the Clinical Center of Serbia for diagnosis and treatment, as are unclear and complicated cases from all across Serbia. Materials and Methods. A retrospective study of trichinellosis serology was carried out from 2009-2018 and included all outpatients and hospitalised patients from the Clinic for Infectious and Tropical Disease, Clinical Center of Serbia, who were serologically tested for Trichinella by the Parasitological Laboratory (n=1,565). Trichinella-specific IgG antibodies were detected in sera by a commercial ELISA test. We analysed the seroprevalence of Trichinella-specific IgG antibodies, antibody detection kinetics and cross-reactivity with other nematodes. Results and Conclusions. The number of patients who reported for serological testing varied greatly per year and month. Most patients were tested in December and March, which coincides with the months with the most confirmed cases of trichinellosis. A total of 17.4% patients who were tested for trichinellosis had other parasitic infections. Altogether, 223 (14.2%) of tested patients were finally diagnosed with trichinellosis. We detected anti-Trichinella IgG in 68.8% (223) of patients with suspected trichinellosis on admission, which increased to 86.5%, 91.5% and 92.4% after later second, third and fourth testing, respectively. Final diagnoses of toxocariasis, strongyloidiasis, filariasis, and dirofilariasis were made for 2.4%, 0.3%, 0.3% and 0.1% of patients, respectively. Concurrent seropositivity for Trichinella and Toxocara was observed in 18.9% (7/37) of patients with clinical presentation of trichinellosis and who were also tested for toxocariasis. In 3/5 patients with imported filariasis, we found cross-reactivity with Trichinella. Potential cross-reactivity of this ELISA test with antibodies to the autochthonous nematode Toxocara canis demands the introduction of Western blot technology. Trichinellosis must be diagnosed by the combination of clinical, laboratory and epidemiological criteria.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49315555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}