Frontiers in Life Sciences and Related Technologies最新文献_第4页

DETERMINATION OF HEAT SHOCK PROTEINS IN SOME BACILLUS SPECIES 几种芽孢杆菌中热休克蛋白的测定

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-08-18 DOI: 10.51753/flsrt.1110386

Elçin YENİDÜNYA KONUK

{"title":"DETERMINATION OF HEAT SHOCK PROTEINS IN SOME BACILLUS SPECIES","authors":"Elçin YENİDÜNYA KONUK","doi":"10.51753/flsrt.1110386","DOIUrl":"https://doi.org/10.51753/flsrt.1110386","url":null,"abstract":"This study investigated the effects of four different temperatures on the sporulation and development of 6 Bacillus species, 2 native and 4 reference. The SDS PAGE analysis showed that two different proteins, 40 and 39 kilodaltons, were produced by B. sphaericus ATCC 2362 after 24 and 48 hours at 48 °C, by local isolate 4 after 24 and 48 hours at 42 °C and by local isolate 31 after 24 and 48 hours at 48 °C. Additionally B. firmus ATCC 14573 produced a 40 kDa protein after 48 hours at 52 °C and B. thuringiensis var. israelensis produced a 42 kDA protein after 48 hours at 42 °C. At temperatures of 48 and 52 °C, after 12, 24 and 48 hours incubation, vegetative and heat resistant spore counts were determined to reduce by 104-106 fold according to bacterial counts. \u0000 In conclusion, the data obtained in this study showed that at 48 and 52 °C spore vitality fell by a significant degree. Additionally the SDS PAGE analysis results revealed different heat shock proteins providing high temperature resistance with a 40 kDA protein produced by B. firmus ATCC 14573, 40-39 kDa proteins produced by B. sphaericus ATCC 2362, and local isolates 4 and 31 and 42kDA protein produced by B. thuringiensis var. israelensis.","PeriodicalId":303081,"journal":{"name":"Frontiers in Life Sciences and Related Technologies","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131354875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINATION OF EXTRACELLULAR HYDROLYTIC ENZYME CAPABILITIES OF SOME ANOXYBACILLUS ISOLATED FROM HOT SPRING ENVIRONMENTS 从温泉环境中分离的一些缺氧杆菌胞外水解酶能力的测定

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-08-18 DOI: 10.51753/flsrt.1094629

Widad Hassan JARWAİS JAF, Emre Erez, Metin Ertaş

{"title":"DETERMINATION OF EXTRACELLULAR HYDROLYTIC ENZYME CAPABILITIES OF SOME ANOXYBACILLUS ISOLATED FROM HOT SPRING ENVIRONMENTS","authors":"Widad Hassan JARWAİS JAF, Emre Erez, Metin Ertaş","doi":"10.51753/flsrt.1094629","DOIUrl":"https://doi.org/10.51753/flsrt.1094629","url":null,"abstract":"Biotechnology has grown immensely in recent years and the production of microbial enzymes was a necessary event in the industrial sectors. The enzymes and the whole cell of microorganisms have also been much used for bioremediation processes and waste management. The main industries that apply microbial enzymes are the food, textile, pharmaceutical, leather, paper, cosmetics, biomaterials, fine chemicals, energy, cellulose and detergent industries. This tool enables researchers to screen for novel bacterial strains that may have a potential enzymatic activity which have not been documented before and for looking plasmids that could serve as cloning vectors to drug resistance in thermophilic microorganism. \u0000In this study the Anoxybacillus flavithermus bacteria were used which was isolated from hot spring of Afyon city from Turkey. The bacteria were tested for the morphological and physiological aspect and analyzed by 16S rRNA comparison for identifications. In this thesis, the biochemical tests were done for identify the characteristics of the bacterium. For this purpose, starch, casein, xylanase and asparaginase hydrolysis and catalase, urease and lipase activities were examined. Also antibiotic resistant tests were performed to the isolated bacteria by using four different (chloramphenicol, ampicillin, rifamycine and erythromycin) antibiotics. The bacterium grows in nutrient agar media, and according to results; all isolated strains fermented starch as carbon and energy sources, and amylase activity was obtained at 50 °C at pH 7.0 after 24 h of the incubation. All strains were evaluated as catalase-positive, most of A. flavithermus strains were obtained as urease and caseinase positive except few strains and especially strain no. KJ095001 was founded urease and caseinase negative.","PeriodicalId":303081,"journal":{"name":"Frontiers in Life Sciences and Related Technologies","volume":"412 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132779045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of LED’s CCT values on the growth characteristics of Triticum aestivum L. (Wheat) and Hordeum vulgare L. (Barley) LED的CCT值对小麦和大麦生长特性的影响

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-08-17 DOI: 10.51753/flsrt.1085027

M. Şahin, Zehra KARAGÖZ KÜÇÜK, M. Elveren, E. Osma, Y. Akaltun

{"title":"The Effects of LED’s CCT values on the growth characteristics of Triticum aestivum L. (Wheat) and Hordeum vulgare L. (Barley)","authors":"M. Şahin, Zehra KARAGÖZ KÜÇÜK, M. Elveren, E. Osma, Y. Akaltun","doi":"10.51753/flsrt.1085027","DOIUrl":"https://doi.org/10.51753/flsrt.1085027","url":null,"abstract":"LEDs are used extensively in almost everywhere in our daily life and they have different color temperatures. The color temperature i.e. CCT (Correlated Color Temperature) is represented by °K (Kelvin). In this study, wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) were cultivated in laboratory environment under LED with different CCT values (2000, 3000, 6000 °K). Plant height measurement, weight values, the quantity of chlorophyll, carotene the amount of leaking of electrolyte and element absorption capacity from the soil, POX and (peroxidase) and SOD (Superoxide dismutase) enzyme activities of cultivated plants were determined. Results were evaluated on SPSS Statistical Package Program and significant differences were obtained on plants which were grown at different color temperatures of light. It was concluded that measurements from plants which were grown under LED light with cold color temperatures (6000 °K) were more consistent than those of plants grown under other lights with different temperatures. Cold color temperatures were also closer to the optimum light values that are required for a plant to grow faster.","PeriodicalId":303081,"journal":{"name":"Frontiers in Life Sciences and Related Technologies","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123793366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Monitoring of heavy metals and essential trace elements in aquatic plant Ranunculus sphaerospermus Boiss. & Blanche (Ranunculaceae), sediments, and water of volcanic Haydarlar Lake, Turkey 水生植物毛茛重金属和必需微量元素的监测。& Blanche(毛茛科)，沉积物和土耳其海达尔火山湖的水

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-08-17 DOI: 10.51753/flsrt.1091165

Faruk Karahan

{"title":"Monitoring of heavy metals and essential trace elements in aquatic plant Ranunculus sphaerospermus Boiss. & Blanche (Ranunculaceae), sediments, and water of volcanic Haydarlar Lake, Turkey","authors":"Faruk Karahan","doi":"10.51753/flsrt.1091165","DOIUrl":"https://doi.org/10.51753/flsrt.1091165","url":null,"abstract":"Aquatic ecosystems contain communities of organisms that are dependent on each other and on their environment. Monitoring of trace element and heavy metal concentrations is important to understand the possible environmental risks in natural aquatic environments. In the present study, concentrations of some heavy metals and trace elements in aquatic plant Ranunculus sphaerospermus, sediments, and water samples of Volcanic Haydarlar Lake were analysed by using ICP-OES. The concentrations were found in the following ranges: 82.11 – 97.38, 9174.50 – 9942.29, and 0.63 – 0.89 for Al; 10.29 – 17.43, 30.60 – 55.60, and 0.81 – 0.98 for B; 1038.44 – 1682.30, 4017.26 – 4503.54, and 1276.61 – 1541.41 for Ca; 120.69 – 178.41, 6894.50 – 8103.47, and 0.51 – 0.69 for Fe; 2503.51 – 2983.38, 1118.50 – 1693.38, and 69.43 – 93.82 for K; 563.38 – 783.22, 885.32 – 1122.47, and 108.55 – 143.36 for Mg in the plant (mg kg−1), sediment (mg kg−1), and water (mg L−1) samples, respectively. The concentrations of Al, Ca, Fe, and K elements in sediments and the content of Ca and K in lake water samples were found as higher than the acceptable limit, while concentrations of all elements in R. sphaerospermus were determined to be within acceptable limits. Transfer factors (TF) of the heavy metal and essential elements from sediment to the plant samples were evaluated. The trends of TF for all samples studied were in the following order: K>Mg>B>Ca>Fe>Al. Consequently, the approach used in this study could contribute to pollution monitoring in the future.","PeriodicalId":303081,"journal":{"name":"Frontiers in Life Sciences and Related Technologies","volume":"284 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134101204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Domates (Lycopsersicum esculentum L.)’te Sentetik Tohum Üretiminde Aljinat Oranlarının Depolama Zamanına Etkisi 藻酸盐比例对番茄（Lycopsersicum esculentum L.）合成种子生产中贮藏时间的影响

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-04-10 DOI: 10.51753/flsrt.1041120

Zafer Secgi̇n, Ahmet Okumuş

引用次数: 1

ANALYSIS OF THE DIAGNOSTIC RESULTS OF COVID-19 INFECTION IN BISHKEK BY REAL-TIME PCR METHOD 比什凯克市新型冠状病毒感染实时PCR诊断结果分析

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-03-13 DOI: 10.51753/flsrt.1050857

Aycan Sadirbaeva, Kadyrbai Chekirov

引用次数: 0

Genotype × environment interaction and adaptation of cowpea genotypes across six planting seasons 6个种植季节豇豆基因型与环境的相互作用及适应性

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-02-20 DOI: 10.51753/flsrt.1036051

A. Ajayi, A. Gbadamosi, O. Osekita, B. Taiwo, Ato Babawole Fawibe, Iyanu Adedeji, Temitope Omi̇saki̇n

引用次数: 1

Citric acid production using rotating biodisc reactor (RBR) 旋转生物盘反应器(RBR)生产柠檬酸

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-01-22 DOI: 10.51753/flsrt.1035228

U. Sidal

引用次数: 1

Seçilmiş Bir Sektördeki İç Ortam Hava Kalitesinin İşçi Sağlığı ve İş Güvenliği Temelinde Değerlendirilmesi

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-01-18 DOI: 10.51753/flsrt.1024910

Ahmet Gökcan, H. Demir, Cevriye Ebrar Aktaş, Rabia Gür, Hatice Uygun, Göksel Demir

引用次数: 0

The Genus Dichodon (Caryophyllaceae) in Turkey 土耳其的石竹属(石竹科)

Frontiers in Life Sciences and Related Technologies Pub Date : 2022-01-09 DOI: 10.51753/flsrt.1000995

M. Keskin

引用次数: 0