Dyes and Pigments最新文献

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BODIPY fluorescent dyes for selective staining of intracellular organelles
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-02-04 DOI: 10.1016/j.dyepig.2025.112688
Mladena Glavaš , Lorena Vidoša , Martina Mušković , Ivana Ratkaj , Nikola Basarić
{"title":"BODIPY fluorescent dyes for selective staining of intracellular organelles","authors":"Mladena Glavaš ,&nbsp;Lorena Vidoša ,&nbsp;Martina Mušković ,&nbsp;Ivana Ratkaj ,&nbsp;Nikola Basarić","doi":"10.1016/j.dyepig.2025.112688","DOIUrl":"10.1016/j.dyepig.2025.112688","url":null,"abstract":"<div><div>By use of a simple synthetic methodology for peptide coupling by HATU reagent, two BODIPY amides <strong>4</strong> and <strong>5</strong> were synthesized, that were at the <em>meso</em>-position substituted with a morphline moiety targeting lysosomes, or sulfonamide group targeting endoplasmic reticulum (ER), respectively. Furthermore, by use of Cu-catalyzed click chemistry, dye <strong>6</strong> was synthesized from a BODIPY alkyne, that was at the <em>meso</em>-position substituted with a triphenylphosponium salt via a triazol linker, for targeting mitochondria. Photophysical and spectral properties of BODIPY dyes <strong>4</strong>–<strong>6</strong> were characterized in nonpolar, polar aprotic and protic solvent. All compounds in all solvents have high values of fluorescence quantum yields (Φ<sub>F</sub> = 0.5–0.8), and single-exponential decay of fluorescence with singlet excited state lifetimes <em>τ</em> = 2.5–3.8 ns. The substituents in the <em>meso</em>-position that direct the dyes towards different intracellular organelles do not affect the photophysical properties. The dyes showed excellent photostability upon irradiation in aqueous solution with 488 nm laser. Moreover, the stability of amides <strong>4</strong> and <strong>5</strong> was tested with lipases CAL-B and PPL, whereupon they showed hydrolytic stability. The applicability of dyes <strong>4</strong>–<strong>6</strong> in fluorescent staining of organelles in HeLa cell line was tested in colocalization microscopy studies with commercial dyes Lysotracker RED, ER tracer and Mitotracker Red. They showed excellent localization in lysosomes, ER and mitochondria with the values of Pearson's coefficients 0.90, 0.94, and 0.93, respectively. Furthermore, ability of dyes <strong>4</strong>–<strong>6</strong> to stain live HeLa cells was demonstrated. Consequently, BODIPY derivatives <strong>4</strong>–<strong>6</strong> have potential to be used in biology as selective organelle targeting dyes. Moreover, our simple synthetic protocol for the preparation of dyes in principle allows for the synthesis of plethora of different BODIPY derivatives with selective localization in different organelles.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112688"},"PeriodicalIF":4.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Highly fluorescent 3-perylenyldiphenylphosphane compounds: An experimental and theoretical study
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-02-04 DOI: 10.1016/j.dyepig.2025.112690
J. Emilio Expósito , Sergio Lentijo , Gabriel Aullón , Manuel Bardají , Jesús A. Miguel
{"title":"Highly fluorescent 3-perylenyldiphenylphosphane compounds: An experimental and theoretical study","authors":"J. Emilio Expósito ,&nbsp;Sergio Lentijo ,&nbsp;Gabriel Aullón ,&nbsp;Manuel Bardají ,&nbsp;Jesús A. Miguel","doi":"10.1016/j.dyepig.2025.112690","DOIUrl":"10.1016/j.dyepig.2025.112690","url":null,"abstract":"<div><div>The perylene derivative, 3-perylenyldiphenylphosphane (PPh<sub>2</sub>Per, <strong>1</strong>), was prepared and used to synthesize the oxidized P(V) compounds A = PPh<sub>2</sub>Per (A = O (<strong>2</strong>); A = S (<strong>3</strong>)). Substitution reactions led to mononuclear gold(I) complexes [AuX(PPh<sub>2</sub>Per)] (X = Cl (<strong>4</strong>); X = C<sub>6</sub>F<sub>5</sub> (<strong>5</strong>)), [AuX(S=PPh<sub>2</sub>Per)] (X = Cl (<strong>6</strong>); X <strong>=</strong> C<sub>6</sub>F<sub>5</sub> (<strong>7</strong>)) and palladium(II) complexes <em>trans</em>-[Pd(C<sub>6</sub>F<sub>5</sub>)<sub>2</sub>(PPh<sub>2</sub>Per)<sub>2</sub>] (<strong>8</strong>). X-ray single crystal studies of compounds <strong>5</strong> and <strong>7</strong> confirmed the expected structures. The UV–Vis absorption spectra display intense peaks in the visible region with maxima from 454 to 461 nm. A DFT study was performed for the absorption spectra of ligands and complexes, showing that the lowest most intense transition is a HOMO → LUMO transition in the perylene core, although affected by functional group and metallic fragment. The ligands and their complexes are fluorescent in solution, due to the perylene fragment, showing an emission in the range 450–550 nm, with maxima from 467 to 472 nm. Quantum yield starts at 13 % for the phosphane and increases dramatically to the range of 63–87 % after oxidation or coordination to a metal fragment. This work illustrates how the PET effect can be used to recover the initial extremely intense emission of free unfunctionalized perylene ring system.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112690"},"PeriodicalIF":4.1,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatiotemporal dynamic monitoring of protein conformation changes by super-resolution imaging
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-02-03 DOI: 10.1016/j.dyepig.2025.112689
Yu Wei , Xuan He , Jiabao Fang , Minzi Ju
{"title":"Spatiotemporal dynamic monitoring of protein conformation changes by super-resolution imaging","authors":"Yu Wei ,&nbsp;Xuan He ,&nbsp;Jiabao Fang ,&nbsp;Minzi Ju","doi":"10.1016/j.dyepig.2025.112689","DOIUrl":"10.1016/j.dyepig.2025.112689","url":null,"abstract":"<div><div>Super-resolution imaging is extensively employed in bioscience research, particularly for monitoring the spatio-temporal dynamics of protein aggregates. Protein aggregates are intricately associated with the pathogenesis of neurodegenerative diseases, tumors, and other illnesses. Traditional optical microscopy fails to capture the intricate structure of protein aggregates. In contrast, super-resolution imaging overcomes the diffraction limit and achieves high spatiotemporal resolution, enabling visualization of the dynamic process of protein aggregation. This paper begins with an overview of the broad meaning of protein aggregates, followed by a summary of fluorescent dyes suitable for super-resolution imaging techniques, including biofluorescent dyes and organic dyes. The focus lies on reviewing research progress pertaining to utilizing super-resolution imaging to investigate the spatio-temporal dynamics of protein aggregates while incorporating different types of super-resolution imaging techniques. In conclusion, super-resolution imaging provides a powerful tool for the study of protein aggregates, which helps to gain insight into the pathological mechanisms of related diseases and provides important information for finding effective therapeutic strategies.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112689"},"PeriodicalIF":4.1,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Full-spectrum luminescence of nitrogen-sulfur co-doped carbon dots and their applications in LED lighting and organelle-lysosome labeling
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-02-01 DOI: 10.1016/j.dyepig.2025.112686
Jing Zhu, Songfang Zhao, Ruifang Guan, Hao Zhang
{"title":"Full-spectrum luminescence of nitrogen-sulfur co-doped carbon dots and their applications in LED lighting and organelle-lysosome labeling","authors":"Jing Zhu,&nbsp;Songfang Zhao,&nbsp;Ruifang Guan,&nbsp;Hao Zhang","doi":"10.1016/j.dyepig.2025.112686","DOIUrl":"10.1016/j.dyepig.2025.112686","url":null,"abstract":"<div><div>Heteroatom doping can achieve specific regulation of carbon dots by changing their nanostructure, electronic structure and band gap. Here, we synthesized nitrogen-sulfur co-doped carbon dots (N, S-CDs) with full-spectrum fluorescence emission capability. Through comprehensive structural analysis using XPS, FTIR, and other characterization techniques, it was revealed that most of the nitrogen atoms were integrated into the carbon core of the N, S-CDs. Meanwhile, sulfur atoms were identified on the surface of the N, S-CDs, incorporated as functional groups. This unique structural configuration provides a foundation for understanding the solvent effect on N, S-CDs. Specifically, as the polarity of the solvent increases, the fluorescence emission of the N, S-CDs experiences a red-shift. The varying hydrogen donor abilities of different solvents contribute to changes in fluorescence emission. Notably, N, S-CDs exhibit near-infrared fluorescence emission when dissolved in acetic acid solvent. Under the synergistic effect of solvent polarity and hydrogen bonding, the fluorescence emission of N, S-CDs achieves full-spectrum luminescence, spanning from blue light to near-infrared. It is particularly interesting to observe that when N, S-CDs are dispersed in polyvinyl alcohol (PVA) films by dissolving them in three different solvents, the red, green, and yellow light emission can be achieved. When coated onto Light Emitting Diode (LED) lamp beads, these films exhibit versatile light emission properties. This provides inspiration for the development and preparation of LED fluorescent materials. Meanwhile, thanks to the excellent luminescent properties of N, S-CDs in water, they can be used for targeted localization of lysosomes in biological cells.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"237 ","pages":"Article 112686"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143452877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Inner filter effect mediated fluorescent sensing of rutin based on amino acid-derived novel nitrogen and sulfur co-doped carbon dots
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-02-01 DOI: 10.1016/j.dyepig.2025.112684
Ze Fang, Xuxin Li, Xin’an Zhang, Wen Yan, Jianxiang Liu, Longshan Zhao
{"title":"Inner filter effect mediated fluorescent sensing of rutin based on amino acid-derived novel nitrogen and sulfur co-doped carbon dots","authors":"Ze Fang,&nbsp;Xuxin Li,&nbsp;Xin’an Zhang,&nbsp;Wen Yan,&nbsp;Jianxiang Liu,&nbsp;Longshan Zhao","doi":"10.1016/j.dyepig.2025.112684","DOIUrl":"10.1016/j.dyepig.2025.112684","url":null,"abstract":"<div><div>Nitrogen and sulfur co-doped carbon dots (N,S-CDs) were constructed through a hydrothermal synthesis employing <span>l</span>-cysteine and <span>d</span>-aspartic acid as precursors in this study. A novel scheme has been proposed for synthesizing N,S-CDs utilizing two amino acids, aiming to detect rutin. The N,S-CDs exhibit excellent luminescence performance as fluorescent sensors with a dimension of 2.5 ± 0.14 nm and excitation/emission wavelengths of 350/429 nm. The rutin quenching mechanisms in N,S-CDs was verified as the inner filter effect (IFE) by spectral analysis, fluorescence lifetime detection, and Parker equation calculation. The system features with N,S-CDs and rutin as the IFE fluorophore and IFE absorber. The fluorescence quenching efficiency of N,S-CDs presented a good linear correlation with rutin concentration in the 20–500 μmol·L<sup>-1</sup> range based on (F<sub>0</sub>–F)/F<sub>0</sub> = 0.0014C + 0.0152 (<em>R</em><sup><em>2</em></sup> = 0.9941) with the detection limit of 8.05 μmol·L<sup>-1</sup>. This method accurately detects the rutin concentration in samples of human urine and serum. The technique developed in this research boasts a wide linear range, fast response time, a wide pH range, excellent stability, and simplicity. It holds a promising potential for the application in the medicine and pharmacy fields.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112684"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cyano substituted stilbene-based polymers for OFETs: From p-type to n-type
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-01-28 DOI: 10.1016/j.dyepig.2025.112685
Kai Gao, Cheng Wang, Kaikai Liu, Haichang Zhang
{"title":"Cyano substituted stilbene-based polymers for OFETs: From p-type to n-type","authors":"Kai Gao,&nbsp;Cheng Wang,&nbsp;Kaikai Liu,&nbsp;Haichang Zhang","doi":"10.1016/j.dyepig.2025.112685","DOIUrl":"10.1016/j.dyepig.2025.112685","url":null,"abstract":"<div><div>The performance of n-type semiconductor materials, typically the charge transport mobility, greatly fall behind compared to those in the p-type ones, which limiting the development of the semiconductor applications. Thus, the molecular design for n-type semiconductor is both crucial and challenging. In this work, three polymers containing isoindigo and stilbene units with varying numbers of cyano-substituted were designed and applied as the semiconductor for the organic field-effect transistors (OFETs). The introduction of cyano groups transforms the stilbene from electron-rich to electron-deficient. As a consequence, the polymers transition from a donor-acceptor type (P0) to an acceptor-acceptor type (P1, P2). With an increasing number of the cyano groups, both of the HOMO and LUMO energy levels of the polymers were decreased, with P1 and P2 exhibiting particularly low LUMO energy levels (&lt;−4.15 eV) and ultra-low HOMO levels (&lt;−5.7 eV). Therefore, P0 exhibited p-type semiconductor behavior with the hole transport mobility of 0.40 cm<sup>2</sup> V<sup>−1</sup> s<sup>−1</sup>, while cyano-substituted P1 and P2 displayed n-type characteristic with 0.62 cm<sup>2</sup> V<sup>−1</sup> s<sup>−1</sup> (P1), and 0.83 cm<sup>2</sup> V<sup>−1</sup> s<sup>−1</sup> (P2), respectively. In addition, the aggregation and molecular packing of the polymers in the thin film state improved with the increasing number of cyano units in the single repeat units, which facilitated interchain charge transport. Thus, P2 exhibited the highest charge transport mobility among the three polymers. This work demonstrates that introducing cyano units into the polymer backbone to synthesize an acceptor-acceptor (A-A) type polymer is a simple and effective strategy for developing n-type semiconductor materials. In addition, incorporating cyano units into the polymer backbone not only increases the electron-deficient character of the polymers and alters their charge transport properties but also might enhance charge transport mobility.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112685"},"PeriodicalIF":4.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Creating a fluorescent “AND” logic gate aptamer platform for identifying exosomes derived from breast cancer subtypes
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-01-27 DOI: 10.1016/j.dyepig.2025.112683
Yifan Liu , Feiran Liu , Zichun Li , Xiaoling Zhang , Jing Jing
{"title":"Creating a fluorescent “AND” logic gate aptamer platform for identifying exosomes derived from breast cancer subtypes","authors":"Yifan Liu ,&nbsp;Feiran Liu ,&nbsp;Zichun Li ,&nbsp;Xiaoling Zhang ,&nbsp;Jing Jing","doi":"10.1016/j.dyepig.2025.112683","DOIUrl":"10.1016/j.dyepig.2025.112683","url":null,"abstract":"<div><div>Breast cancer is a globally prevalent malignancy, and early classification of breast cancer is beneficial for formulating accurate treatment plans and improving prognosis. In this work, we engineered an innovative dual-marker approach, capitalizing on the precision of “AND” logic gates, to precisely identify different subtypes of exosomes derived from breast cancer. We designed two distinct nucleic acid aptamers:PD-L1 (Programmed cell death-ligand 1) aptamer conjugated with Cy3, and EpCAM (Epithelial cell adhesion molecule) aptamer labeled with Cy5. The conjugation of both aptamers and specific connectors culminated in the establishment of a robust detection system. Upon successful binding to breast cancer-derived exosomes, a remarkable Förster Resonance Energy Transfer (FRET) phenomenon transpires, enabling the detection of the target exosomes. Through the optimization of the linker, we extended the application of this method to fluorescence imaging, which is capable of elucidating the interactions between exosomes and immune cells. This not only visualizes the process of exosome internalization into receptor cells but also paves the way for a novel non-invasive diagnostic strategy for breast cancer.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112683"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Preparation of organic pigment dispersants based on comb-shaped acrylic copolymer and their applications
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-01-27 DOI: 10.1016/j.dyepig.2025.112665
Tingwu Liu , Kexin Chen , Jianwei Han , Limin Wang
{"title":"Preparation of organic pigment dispersants based on comb-shaped acrylic copolymer and their applications","authors":"Tingwu Liu ,&nbsp;Kexin Chen ,&nbsp;Jianwei Han ,&nbsp;Limin Wang","doi":"10.1016/j.dyepig.2025.112665","DOIUrl":"10.1016/j.dyepig.2025.112665","url":null,"abstract":"<div><div>Optimizing the structure of comb-shaped acrylic copolymers is essential for developing high-performance organic pigment dispersants in waterborne coatings. In this study, a series of copolymers composed of benzyl methacrylate (BzMA) and poly(ethylene glycol) methyl ether methacrylate (mPEGMA) was synthesized via solution polymerization, maintaining a consistent molecular weight while varying the monomer ratios. The copolymers were comprehensively characterized using gel permeation chromatography (GPC), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy (FTIR), and differential scanning calorimetry (DSC) analysis. Surface properties were investigated by measuring the critical micelle concentration (CMC), micellization energy, and limiting area at the liquid-gas interface as a function of polymer concentration and surface tension. The thickness of the hydration layer was calculated based on the structure, while the micelle size was determined using dynamic light scattering (DLS). Adsorption isotherms of the polymers on phthalocyanine blue were measured to evaluate changes in adsorption amount, adsorption energy, and limiting area at the solid-liquid interface in relation to the monomer ratio. Comparison of the results from adsorption and surface tension tests elucidated polymer conformations at both liquid-gas and solid-liquid interfaces. The dispersing performance of the polymers was also evaluated by testing the viscosity, particle size, and stability of pigment dispersions. This study provides insights into the design of effective polymeric dispersants for waterborne systems.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112665"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Small organic molecules-based NIR agents in cancer diagnostics: New frontiers in imaging and therapy
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-01-27 DOI: 10.1016/j.dyepig.2025.112648
Amal Adnan Ashour , Mohammed Fareed Felemban , Faris J. Tayeb , Abeer Alubaidi , Alaa Shafie
{"title":"Small organic molecules-based NIR agents in cancer diagnostics: New frontiers in imaging and therapy","authors":"Amal Adnan Ashour ,&nbsp;Mohammed Fareed Felemban ,&nbsp;Faris J. Tayeb ,&nbsp;Abeer Alubaidi ,&nbsp;Alaa Shafie","doi":"10.1016/j.dyepig.2025.112648","DOIUrl":"10.1016/j.dyepig.2025.112648","url":null,"abstract":"<div><div>Cancer is the leading cause of death worldwide, with millions of new cases and fatalities reported each year. Early detection plays a critical role in improving treatment outcomes and survival rates, as it allows for timely intervention and more effective therapies. Traditional diagnostic methods often face limitations in sensitivity, specificity, and the ability to detect tumors at an early stage. Therefore, there is a growing need for advanced diagnostic tools that can offer higher precision and earlier detection of cancer. In this regard, Near-Infrared (NIR) imaging has emerged as a promising technique, offering non-invasive, real-time, and high-resolution imaging capabilities for tumor visualization. Small organic molecules-based NIR agents have recently garnered attention in the field of cancer diagnostics due to their superior biocompatibility, tunable properties, and ability to penetrate deeper into tissues. These agents, including various organic fluorophores, that are designed to enhance the sensitivity and specificity of cancer imaging, enabling the detection of smaller tumors that are otherwise challenging to identify. This review aims to provide an overview of the latest advancements (2020–2025) in small organic molecules-based NIR agents for various cancer diagnosis, focusing on their development, applications, and potential for clinical use. By examining key innovations in this area, this article seeks to highlight the impact of small organic molecules-based NIR agents on the future of cancer theranostics.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112648"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metabolic engineering of Escherichia coli BL21(DE3) for efficient production of indigo using Methylophaga aminisulfidivorans flavin-containing monooxygenase
IF 4.1 3区 工程技术
Dyes and Pigments Pub Date : 2025-01-27 DOI: 10.1016/j.dyepig.2025.112682
Rui Lu , Roulin Chen , Zeyu Li , Heng Hu , Zhimeng Wu , Yingying Zhu , Wanmeng Mu
{"title":"Metabolic engineering of Escherichia coli BL21(DE3) for efficient production of indigo using Methylophaga aminisulfidivorans flavin-containing monooxygenase","authors":"Rui Lu ,&nbsp;Roulin Chen ,&nbsp;Zeyu Li ,&nbsp;Heng Hu ,&nbsp;Zhimeng Wu ,&nbsp;Yingying Zhu ,&nbsp;Wanmeng Mu","doi":"10.1016/j.dyepig.2025.112682","DOIUrl":"10.1016/j.dyepig.2025.112682","url":null,"abstract":"<div><div>Biological production of indigo can be achieved by enzymatic hydroxylation of indole, the degradation product of tryptophan. Many oxygenases have been identified to be capable of producing indigo from indole, among which flavin-containing monooxygenase (FMO) is the most extensively studied. In this study, <em>Escherichia coli</em> BL21 (DE3) was metabolically engineered to efficiently produce indigo using FMO. Five reported indigo-producing FMOs and five homologous human FMOs were selected to evaluate the <em>in vivo</em> indigo production abilities, in which <em>Methylophaga aminisulfidivorans</em> FMO showed the highest indigo titer. Various metabolic engineering strategies were performed to stepwise enhance the indigo titer, including strengthening tryptophan transportation, blocking tryptophan synthesis-related competitive pathways, regulating key metabolic genes for tryptophan synthesis. When supplemented with tryptophan, the final engineered strain produced 532 and 1492 mg/L of indigo by shake-flask and fed-batch cultivation, respectively.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"236 ","pages":"Article 112682"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143167904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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