Dyes and Pigments最新文献_第9页

Advances in near-infrared fluorescence imaging for early detection of acute kidney injury 近红外荧光成像在急性肾损伤早期检测中的研究进展

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-11 DOI: 10.1016/j.dyepig.2025.113231

Wenjing Bai , Wuyue Su , Peng Zhang , Xuechuan Hong , Yuling Xiao , Xiaodong Zeng

{"title":"Advances in near-infrared fluorescence imaging for early detection of acute kidney injury","authors":"Wenjing Bai , Wuyue Su , Peng Zhang , Xuechuan Hong , Yuling Xiao , Xiaodong Zeng","doi":"10.1016/j.dyepig.2025.113231","DOIUrl":"10.1016/j.dyepig.2025.113231","url":null,"abstract":"<div><div>Acute kidney injury (AKI) is a severe clinical condition with complex pathogenesis and high morbidity. Early diagnosis is vital for improving outcomes, but traditional markers such as serum creatinine and urine output lack sensitivity and timeliness. Near-infrared (NIR) fluorescence imaging has emerged as a promising tool for early AKI detection due to its deep tissue penetration, high sensitivity, and real-time monitoring capability. This review summarizes the design strategies of NIR fluorescent probes, including fluorophore optimization, linker engineering, and targeting group selection. We focus on their applications in detecting AKI biomarkers, such as Vanin-1, KIM-1, and LAP, which enable sensitive detection in urine or kidney tissue through enzymatic or receptor-mediated mechanisms. Probes targeting oxidative stress markers like reactive oxygen species and peroxynitrite further allow dynamic visualization of early AKI-related changes. Despite their advantages, clinical translation of NIR probes faces challenges, including biocompatibility, targeting precision, imaging depth, and integration with multimodal platforms. Continued optimization of probe design is essential to advance their use in early AKI diagnosis and precision medicine.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113231"},"PeriodicalIF":4.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Asymmetric phthalimide-based conjugated monomer synthesis in a flow reactor 流动反应器中不对称邻苯二胺基共轭单体的合成

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-11 DOI: 10.1016/j.dyepig.2025.113236

Nayeon Kim, Chae Yeon Park, Ye-Jin Hwang

{"title":"Asymmetric phthalimide-based conjugated monomer synthesis in a flow reactor","authors":"Nayeon Kim, Chae Yeon Park, Ye-Jin Hwang","doi":"10.1016/j.dyepig.2025.113236","DOIUrl":"10.1016/j.dyepig.2025.113236","url":null,"abstract":"<div><div>An asymmetric design strategy for organic semiconductors enhances device efficiency through effective morphology control while broadening design possibilities. However, asymmetric monomer synthesis in conventional batch reactors is generally more complex than symmetric synthesis, requiring additional reaction steps that typically result in lower selectivity and yields. Here, we propose the flow synthesis of asymmetric monomers as an advantageous alternative. By applying our synthetic method, the steps required to synthesize a target asymmetric monomer can be reduced from five to three. Using a custom-designed flow reactor, we carried out palladium-catalyzed Stille coupling reactions to produce an asymmetric phthalimide (PhI) monomer with thiophene (Th) and bromide substituents on each side (Th-PhI-Br), starting from dibromide phthalimide (PhI-Br<sub>2</sub>). With our highly reproducible synthesis system (the standard deviation of the yield for repeated runs was below 2 %), we screened various reaction conditions, including temperature, reaction time, flow rate, PhI-Br<sub>2</sub> to thiophene ratio, and reactant injection sequence. We then examined the correlations between these conditions and the reaction selectivity and yield. Our results demonstrate that the flow synthesis process is an effective method for optimizing selectivity and yield in the production of asymmetric conjugated monomers.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113236"},"PeriodicalIF":4.2,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multistate second-order nonlinear optical switches based on dimethyldihydropyrene-azobenzene hybrid system 基于二甲基二氢芘-偶氮苯杂化体系的多态二阶非线性光开关

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-10 DOI: 10.1016/j.dyepig.2025.113232

Yao Yao , Hong-Qing Wang , Feng-Yi Zhang , Hong-Liang Xu , Chun-Guang Liu

{"title":"Multistate second-order nonlinear optical switches based on dimethyldihydropyrene-azobenzene hybrid system","authors":"Yao Yao , Hong-Qing Wang , Feng-Yi Zhang , Hong-Liang Xu , Chun-Guang Liu","doi":"10.1016/j.dyepig.2025.113232","DOIUrl":"10.1016/j.dyepig.2025.113232","url":null,"abstract":"<div><div>This study pioneers the application of quantum chemical methods to systematically elucidate the evolution of second-order nonlinear optical (NLO) responses in multistate molecular switches derived from a dual photochromic dimethyldihydropyrene (DHP)-azobenzene (AB) hybrid system during isomerization processes. Through time-dependent density functional theory (TDDFT) simulations, we unravel the intricate structure-property correlations and establish a theoretical framework for molecular-level control of NLO performance. The results reveal that, in this hybrid system, a cascade isomerization pathway (E-C ↔ Z-C ↔ Z-O → E-O ↔ E-C) can be induced by wavelength-selective photoexcitation and thermal treatment, exhibiting efficient “ON–OFF–OFF–ON–ON” NLO switching behavior. The derivatives featuring the E-C structure exhibit the largest dynamic <em>β</em><sub>HRS</sub> value (8.4 × 10<sup>4</sup> a.u.) in acetonitrile, surpassing that of the conventional DO3 system by a factor of 5.7, attributed to enhanced intramolecular charge transfer (ICT) and extended π-conjugation. We find that the donor-DHP/acceptor-AB linkage (Series 1 compounds) yields optimal NLO performance, with <em>β</em><sub>HRS</sub> values 32–243 % higher than those of the reversed configuration, offering a viable design strategy for high-performance NLO materials. The DHP-AB hybrid system emerges as a promising NLO switch candidate for multilevel optical memory devices with non-destructive readout capabilities. Our findings provide a molecular design blueprint for tunable NLO switches, enabling development of advanced optoelectronic technologies.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113232"},"PeriodicalIF":4.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NIR-II aza-BODIPY photosensitizers with enhanced ICT effect for photothermal therapy of breast cancer 具有增强ICT效应的NIR-II aza-BODIPY光敏剂用于乳腺癌光热治疗

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-10 DOI: 10.1016/j.dyepig.2025.113230

Zhijie Fang, Han Yu, Liuqi Wang, Zihang Zeng, Weiqing Yue, Xiaomei Lu, Jie Li

{"title":"NIR-II aza-BODIPY photosensitizers with enhanced ICT effect for photothermal therapy of breast cancer","authors":"Zhijie Fang, Han Yu, Liuqi Wang, Zihang Zeng, Weiqing Yue, Xiaomei Lu, Jie Li","doi":"10.1016/j.dyepig.2025.113230","DOIUrl":"10.1016/j.dyepig.2025.113230","url":null,"abstract":"<div><div>Enhancing intramolecular charge transfer (ICT) is a well-established approach to achieving red-shifted absorption and emission in aza-BODIPY derivatives. However, highly planar π-conjugated structures, commonly employed to strengthen ICT, often induce strong intermolecular π–π stacking, leading to reduced quantum yields, and consequently diminishing the overall photophysical performance. We introduced moderately electron-donating, non-planar 1-ethyl-1,2,3,4-tetrahydroquinoline units at the 1,7-positions and further enhanced donor strength at the 3,5-positions of the aza-BODIPY core to synthesized a series of aza-BODIPY photosensitizers: <strong>NJ1088</strong>, <strong>NJ1100</strong>, and <strong>NJ1150</strong>. <strong>NJ1150</strong> displayed a pronounced absorption peak at 967 nm and retained substantial absorption beyond 1100 nm. Density Functional Theory (DFT) calculations confirmed that <strong>NJ1150</strong> has a reduced HOMO–LUMO gap of 1.45 eV, consistent with its long-wavelength absorption. Moreover, the observed increase in Stokes shifts correlated with stronger donor groups further corroborates the intensified ICT effect in the series. Fluorescence studies revealed that <strong>NJ1150</strong> exhibited a relatively high quantum yield of 1.89 % in DCM. Photothermal conversion studies demonstrated that <strong>NJ1150</strong> encapsulated in PLX nanoparticles (<strong>NJ1150@PLX</strong>) reached 64 % conversion efficiency under 1064 nm laser irradiation. <em>In vitro</em> assays confirmed that 1064 nm laser irradiation of <strong>NJ1150@PLX</strong> induced significant tumor-cell apoptosis. Finally, evaluation in an orthotopic breast cancer mouse model revealed that <strong>NJ1150@PLX</strong> provided excellent second near-infrared window (NIR-II) fluorescence imaging and effectively suppressed tumor growth <em>via</em> photothermal therapy.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113230"},"PeriodicalIF":4.2,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decoding emission pathways in solid-state salicylaldimines 解码固态水杨醛二胺的发射途径

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-09 DOI: 10.1016/j.dyepig.2025.113228

Olaf Morawski , Pawel Gawrys , Joanna Olas , Sergiu Shova , Kinga Suwińska , Cristina A. Barboza

{"title":"Decoding emission pathways in solid-state salicylaldimines","authors":"Olaf Morawski , Pawel Gawrys , Joanna Olas , Sergiu Shova , Kinga Suwińska , Cristina A. Barboza","doi":"10.1016/j.dyepig.2025.113228","DOIUrl":"10.1016/j.dyepig.2025.113228","url":null,"abstract":"<div><div>This study examines six salicylaldimines in their crystalline form, analyzing their photoluminescent properties at both room and liquid helium temperatures (5 K). Obtained results provide a solid-state perspective on the photophysical characteristics of a class of donor-acceptor compounds that were previously analyzed in different solvents (<em>Journal of Molecular Liquids</em>, 2021, 343, 117532). Consistent with measurements performed in solution, four donor-acceptor salicylaldimines (<strong>1</strong>–<strong>4</strong>) exhibit solely LE emission, while analogous naphthyl derivatives (<strong>5</strong> and <strong>6)</strong> exhibit ESIPT occurrence in the solid state. This finding correlates strongly with the estimated height of the ESIPT reaction barrier computed at the ADC(2)/cc-pVDZ level of theory. Consequently, <strong>1</strong>–<strong>4</strong> deviate from the traditional classification of salicylideneanilines into thermochromic or photochromic materials. In contrast, <strong>5</strong> and <strong>6</strong> show weak dual fluorescence with the dominant ESIPT emission. The crystal packing restricts large-amplitude motions, resulting in quantum yields over one order of magnitude higher than those in solution. Interestingly, the anisotropy of the polycrystal favors the antiparallel stacking of <strong>1</strong>–<strong>5</strong>, which promotes the population of a dark CT state upon excitation, which competes with the LE emission at low temperature, manifested by the observed growth of LE fluorescence intensity shortly after excitation.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113228"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial light-harvesting systems for photoluminescent and electroluminescent white organic light-emitting materials 光致发光和电致发光白色有机发光材料的人工光收集系统

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-09 DOI: 10.1016/j.dyepig.2025.113229

Yu Li , Honghan Ji , Bingyan Liu , Zijie Xu , Wenzeng Duan , Wenjun Wang , Shuhong Li

{"title":"Artificial light-harvesting systems for photoluminescent and electroluminescent white organic light-emitting materials","authors":"Yu Li , Honghan Ji , Bingyan Liu , Zijie Xu , Wenzeng Duan , Wenjun Wang , Shuhong Li","doi":"10.1016/j.dyepig.2025.113229","DOIUrl":"10.1016/j.dyepig.2025.113229","url":null,"abstract":"<div><div>Achieving efficient and stable light emission across a broad spectrum remains a critical challenge in the development of advanced organic optoelectronic materials and devices. In this study, [2.2]paracyclophane-based triphenylamine (<strong>PCP-TPA</strong>) and phenyl-based triphenylamine (<strong>Ph-TPA</strong>) were prepared and can both be used to construct innovative artificial light-harvesting systems (ALHSs) to arbitrarily regulate the emission in the visible light region. These molecules exhibit exceptional energy transfer efficiency compared to other dyes, enabling the achievement of white light emission in both solution and PMMA film. By carefully designing molecular interactions and optimizing energy transfer processes, <strong>PCP-TPA</strong> is applied to fabricate a cool white organic light-emitting diode (WOLED) with a turn-on voltage (V<sub>on</sub>) of 2.3 V, accompanied by a maximum luminance (<em>L</em><sub>max</sub>) of 1110 cd/m<sup>2</sup>. These results highlight the significant potential of [2.2]paracyclophane derivatives in advancing the development of stable and efficient white light-emitting optoelectronic materials, and describe a method of optimizing the design of advanced light-harvesting system through molecular engineering and energy transfer optimization.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113229"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hyperbranched MR-TADF polymers with rigid-flexible balance engineering for solution-processed flexible narrowband OLEDs 超支化MR-TADF聚合物溶液加工柔性窄带oled的刚柔平衡工程

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-09 DOI: 10.1016/j.dyepig.2025.113225

Hongyu Zhuang , Wenhao Zhang , Wenli Shi, Jiangqi Jiang, Hai Zhou, Yumeng Xin, Xinxin Ban

{"title":"Hyperbranched MR-TADF polymers with rigid-flexible balance engineering for solution-processed flexible narrowband OLEDs","authors":"Hongyu Zhuang , Wenhao Zhang , Wenli Shi, Jiangqi Jiang, Hai Zhou, Yumeng Xin, Xinxin Ban","doi":"10.1016/j.dyepig.2025.113225","DOIUrl":"10.1016/j.dyepig.2025.113225","url":null,"abstract":"<div><div>Although multiple resonance thermally activated delayed fluorescence (MR-TADF) materials have emerged as a research hotspot in organic light-emitting diodes (OLEDs) due to their narrowband emission and high exciton utilization efficiency, their rigid planar structures hinder solution processability and flexible film fabrication. Herein, a “rigid-flexible balance” molecular engineering strategy is proposed to design and synthesize three-dimensional dendritic polymer materials for the first time, featuring MR-TADF units as the core and diphenyl fluorene as block units. The dendritic topology effectively suppresses intermolecular π-π stacking through steric hindrance from the block units, enhancing photoluminescence quantum yield to 60 % and reducing non-radiative transitions. The unique non-conjugated flexible linkage architecture not only preserves the high color purity of the deep blue MR core (CIE: 0.14, 0.12) but also significantly reduces backbone rigidity, endowing the material with superior solution processability and mechanical flexibility. This design, combined with PVK, can form excellent interloped films to further improve the device's stability under bending conditions, solving a key challenge for traditional rigid MR-TADF materials in flexible applications. The OLEDs based on this hyperbranched polymer exhibit a low turn-on voltage of 4.2 V, high external quantum efficiency (EQE) of 8.5 %, and narrow emission with FWHM of 52 nm. Remarkably, even after repeated bending of flexible devices, they retain an EQE of 5.9 % with maintained narrowband characteristics. This work provides an innovative solution to overcome the limitations of planar MR-TADF materials, highlighting the unique advantages of hyperbranched polymers in flexible OLEDs.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113225"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A near-infrared anionic heptamethine cyanine for siRNA-sensitized photothermal therapy 用于sirna敏化光热治疗的近红外阴离子七甲基氰菁

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-09 DOI: 10.1016/j.dyepig.2025.113227

Yikun Li , Fuping Han , Haiqiao Huang , Danhong Zhou , Saran Long , Wen Sun , Jianjun Du , Jiangli Fan , Xiaojun Peng

{"title":"A near-infrared anionic heptamethine cyanine for siRNA-sensitized photothermal therapy","authors":"Yikun Li , Fuping Han , Haiqiao Huang , Danhong Zhou , Saran Long , Wen Sun , Jianjun Du , Jiangli Fan , Xiaojun Peng","doi":"10.1016/j.dyepig.2025.113227","DOIUrl":"10.1016/j.dyepig.2025.113227","url":null,"abstract":"<div><div>Photothermal therapy (PTT), though effective for tumor cell ablation, is hindered in clinical applicability due to hyperthermia injury caused by excessive temperatures. Mild photothermal therapy (mPTT) presents a promising alternative with reduced side effects. As pivotal components of mPTT, the performance optimization of photothermal agents (PTAs) hold critical importance. In this study, we developed an <em>N</em>-methylpiperazine-modified anionic Cy7 (<strong>CyNM</strong>) that achieved a 5.4-fold enhancement in photothermal conversion efficiency over the parent Cy7 through an intramolecular charge transfer (ICT) strategy. To amplify therapeutic outcomes, we engineered <strong>CyNPH</strong> by integrating <strong>CyNM</strong> and anti-Hsp70 siRNA to suppress heat shock protein (HSP) activity during mPTT. Moreover, <strong>CyNPH</strong> demonstrated dual-modal fluorescence/photoacoustic (PA) imaging-guided siRNA-sensitized PTT for breast cancer. Notably, this work offers novel insights into the rational design of anionic PTAs and image-guided mPTT, and demonstrates substantial clinical translation potential, thereby providing a simple and controllable treatment strategy.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113227"},"PeriodicalIF":4.2,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quinoxaline-derived aza-BODIPY AIEE fluorescent probes for lysosomal viscosity imaging 喹诺啉衍生的aza-BODIPY AIEE荧光探针用于溶酶体粘度成像

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-06 DOI: 10.1016/j.dyepig.2025.113220

Ru Feng , Zuoxu Chen , Xinhao Zhu , Zhaoli Xue , Jianming Pan , Yue Wang , Jiangwei Tian

{"title":"Quinoxaline-derived aza-BODIPY AIEE fluorescent probes for lysosomal viscosity imaging","authors":"Ru Feng , Zuoxu Chen , Xinhao Zhu , Zhaoli Xue , Jianming Pan , Yue Wang , Jiangwei Tian","doi":"10.1016/j.dyepig.2025.113220","DOIUrl":"10.1016/j.dyepig.2025.113220","url":null,"abstract":"<div><div>In this study, two novel quinoxaline-derived aza-BODIPY fluorescent probes (<strong>1</strong> and <strong>2</strong>) responsive to lysosomal viscosity were synthesized via a one-pot Schiff base reaction. Both probes feature a rotatable benzothiazole group linked via a C<img>N bond, while probe <strong>2</strong> incorporates a morpholine moiety to enhance lysosomal targeting. Single-crystal X-ray diffraction confirmed that the morpholine group increases π-electron delocalization and affects molecular stacking. Both probes exhibited characteristic UV–visible absorption and emission with large Stokes shifts. Probe <strong>2</strong> showed a red-shift due to the electron-donating effect of morpholine. Aggregation-induced emission enhancement (AIEE) was observed in both probes. Both displayed strong linear fluorescence responses to viscosity (2–610 cP), with up to 24-fold enhancement and low detection limits (0.27 and 0.26 cP). They demonstrated excellent selectivity over biological interferents and negligible cytotoxicity in MTT assays. Co-localization experiments revealed better lysosomal targeting for probe <strong>2</strong> (R = 0.86) compared to probe <strong>1</strong> (R = 0.83), attributed to the dual-targeting effect of the benzothiazole and morpholine groups. Both probes successfully monitored dexamethasone-induced lysosomal viscosity changes (10–40 μM) in live cells, enabling real-time tracking of the lysosomal microenvironment. Probe <strong>2</strong> consistently showed stronger fluorescence intensity than probe <strong>1</strong>, highlighting its superior sensitivity and detection performance. These findings suggest the potential of these aza-BODIPY-based probes as effective tools for real-time intracellular viscosity imaging and lysosome-related disease research.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113220"},"PeriodicalIF":4.2,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145095741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel tyrosinase-ascorbate system for fluorescence detection of equine doping adrenaline 一种新的酪氨酸酶-抗坏血酸荧光检测系统

IF 4.2 3区工程技术

Dyes and Pigments Pub Date : 2025-09-06 DOI: 10.1016/j.dyepig.2025.113222

Yanling Cen , Yuanyan Zuo , Shiji Miao , Shijian Li , Zhiqiang Xu , Qi Sun , Zhichao Ma , Genyan Liu

{"title":"A novel tyrosinase-ascorbate system for fluorescence detection of equine doping adrenaline","authors":"Yanling Cen , Yuanyan Zuo , Shiji Miao , Shijian Li , Zhiqiang Xu , Qi Sun , Zhichao Ma , Genyan Liu","doi":"10.1016/j.dyepig.2025.113222","DOIUrl":"10.1016/j.dyepig.2025.113222","url":null,"abstract":"<div><div>The artificial enhancement of equine athletic performance through pharmacological agents remains a critical concern in racing and other equine sports, threatening both animal welfare and the fairness of competition. Adrenaline, a controlled substance in equine doping, enhances explosive power and masks fatigue, yet its detection remains challenging due to its chemical instability and the limitations of conventional mass spectrometry methods. Herein, we report a simple and sensitive fluorescence-based detection strategy using a tyrosinase-ascorbic acid system to improve Ad stability and enable rapid quantification. In this system, TYR efficiently oxidizes Ad to adrenochrome, a fluorescent product with strong yellow-green fluorescence and a large Stokes shift (220 nm), allowing high signal-to-noise detection. And it exhibits excellent sensitivity with a detection limit of 0.69 μM and high selectivity over structurally similar catecholamines. Furthermore, the tyrosinase-ascorbic acid system successfully detects Ad in complex biological matrices, including untreated equine urine, under physiological pH and temperature conditions. This study introduces a practical and innovative fluorescence-based platform for rapid Ad detection, offering a promising alternative to traditional doping assays in equine sports.</div></div>","PeriodicalId":302,"journal":{"name":"Dyes and Pigments","volume":"245 ","pages":"Article 113222"},"PeriodicalIF":4.2,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145019866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0