Biotemas最新文献_第4页

Genetic stability in interspecific hybridizations of wheat populations determined by meiotic index and pollen viability 小麦群体种间杂交的遗传稳定性由减数分裂指数和花粉活力决定

Biotemas Pub Date : 2021-06-01 DOI: 10.5007/2175-7925.2021.E79666

Patricia Frizon, S. Brammer, C. C. Deuner, A. Chechi, M. I. Lima, P. Scheeren

{"title":"Genetic stability in interspecific hybridizations of wheat populations determined by meiotic index and pollen viability","authors":"Patricia Frizon, S. Brammer, C. C. Deuner, A. Chechi, M. I. Lima, P. Scheeren","doi":"10.5007/2175-7925.2021.E79666","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E79666","url":null,"abstract":"The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained by the crosses between wheat commercial cultivars and synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, and they were fixed in Carnoy’s solution. The cytological slides were prepared by crushing the anthers and staining with 1% acetocarmine, and the normal tetrads and the ones with micronuclei were observed under a light microscope. For pollen viability, the ears were collected at the stage of mature pollen grain. The methodological procedure was similar to that of the tetrads, evaluating the pollen grains as viable, with little starch, with two pores, different sizes and non-viable. The pollen grain size was measured by the Axion Vision software. All the segregating populations with MI above 90% and high pollen viability (above 85%) reflected a stable meiotic behavior. About the variation in size, the CIGM90.909/BRS 179 cross showed pollen grains with 58.43 μm and CIGM93.298/BRS Guamirim, 47.15 μm. According to the results, wheat segregating populations were considered meiotically stable and suitable for use in wheat breeding programs and for incorporation of new important genes.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43656031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Ictiofauna do Parque Nacional da Serra da Bodoquena, Mato Grosso do Sul, Brasil: composição e subsídios à conservação 巴西南马托格罗索Serra da Bodoquena国家公园的鱼类：组成和保护补贴

Biotemas Pub Date : 2021-06-01 DOI: 10.5007/2175-7925.2021.E76255

M. E. A. Vilela, Francisco de Paula Severo Neto, Fernando R. Carvalho, Otávio Froehlich

{"title":"Ictiofauna do Parque Nacional da Serra da Bodoquena, Mato Grosso do Sul, Brasil: composição e subsídios à conservação","authors":"M. E. A. Vilela, Francisco de Paula Severo Neto, Fernando R. Carvalho, Otávio Froehlich","doi":"10.5007/2175-7925.2021.E76255","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E76255","url":null,"abstract":"The Serra da Bodoquena includes the headwaters of important rivers of the upper Paraguay River basin and constitutes one of the more extensive karst formations in Brazil. The region is an important tourist destination and Serra da Bodoquena National Park (SBNP) was established in 2000. In this work, we provide a list of the ichthyofauna and describe the main water systems in SBNP. Surveys were carried out in August, September and December 2005 using trawl nets, gill nets and electrofishing. For the list of species, we included records made in subsequent studies, resulting in 60 species belonging to seven orders and 21 families, which are all autochthonous to the upper Paraguay River, except the tilapia Coptodon rendalli, the only non-native species. The species richness was higher in the Salobra River basin (49 species, 39 exclusive) compared to the Perdido River basin (15 species, six exclusive). We documented seven endemic species in the Serra da Bodoquena, of which two are threatened with extinction (Vulnerable category): Trichomycterus dali and Ancistrus formoso. As mitigation and preventive measures for environmental impacts, we suggest regularizing land use and increasing environmental education programs as ways of contributing to public policies that are involved in the conservation of aquatic systems and their biotic communities.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43204557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Floristic composition of Melastomataceae between reforested and natural areas in the Recife Atlantic Forest, Pernambuco, Brazil 巴西伯南布哥累西腓大西洋森林中重新造林和自然区域之间的野牡丹科植物区系组成

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77751

J. Maciel, Yuri de Souza Vieira Couceiro

{"title":"Floristic composition of Melastomataceae between reforested and natural areas in the Recife Atlantic Forest, Pernambuco, Brazil","authors":"J. Maciel, Yuri de Souza Vieira Couceiro","doi":"10.5007/2175-7925.2021.E77751","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E77751","url":null,"abstract":"Local extinction of native Atlantic Forest flora species occurs mainly due to habitat fragmentation. Reforestation and natural regeneration can mitigate these factors. The present study attempts to understand whether floristic composition and dispersal processes are being reestablished in an area of lowland Atlantic Forest, eight years after its reforestation. The study area was an old pasture set between two urban fragments in the city of Recife that was reforested in 2011. Melastomataceae species were collected in this area and the morphology of their fruits was compared with the species of the family recorded in the adjacent fragments. Miconia prasina, M. albicans, M. affinis, Clidemia hirta, and C. capitellata have been found in the reforested area. Although there are species with larger fruits in the adjacent fragments, there was no statistically significant difference between the means of the two areas. The fruit morphology indicates that the species found in the area are dispersed by birds and small mammals. It is possible to conclude that the reforested area already presents the expected ecological processes for a corridor between two fragments. Thus, the two adjacent areas serve as vectors for the colonization of native Atlantic Forest species in the reforested area.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46609147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Otimização das condições de cultivo do fungo filamentoso Fusarium sp. EA 1.3.7 para a produção de xilanases 丝状真菌Fusarium sp.EA1.3.7产木聚糖酶培养条件的优化

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77440

Eloísa Alves Nogueira, Tarcisio Michael Ferreira Soares De Oliveira, Ingrid Cristina Santos Amorim, Tássio Britto De Oliveira, D. L. Nelson, Vivian Machado Benassi

引用次数: 2

Evaluation of original Roundup® effects on Biomphalaria glabrata (Say, 1818) Roundup®对光滑生物阴茎的原始影响评估（Say，1818）

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77320

Marta Júlia Faro, V. S. Moura, R. Augusto, M. Vasconcellos

{"title":"Evaluation of original Roundup® effects on Biomphalaria glabrata (Say, 1818)","authors":"Marta Júlia Faro, V. S. Moura, R. Augusto, M. Vasconcellos","doi":"10.5007/2175-7925.2021.E77320","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E77320","url":null,"abstract":"The objective of this work is to evaluate the action of original Roundup® on the snail Biomphalaria \u0000glabrata infected and not infected by Schistosoma mansoni by observing its mortality and reproductive biology. \u0000Snails were submitted for 30 days to aqueous solutions of original Roundup at concentrations of 0.1, 0.09, \u00000.08, 0.07, 0.06, 0.05, 0.04, 0.03, 0.02, 0.018, 0.016, 0.014, 0.012 and 0.01 (%) to determine LC50. To analyze the reproductive activity of B. glabrata, against the pesticide, four B. glabrata groups were formed: infected \u0000and exposed to the pesticide; infected and not exposed (control); not infected and exposed; and not infected \u0000and not exposed(control). LC50 (0.012% aqueous solution of original Roundup) caused mortality of at most up \u0000to 95% for 24, 48 and 72 h during the experimental period (30 days), and lost effect totally after 27 days. The \u0000reproductive activity of the non-infected and exposed snails was 30.3 ± 20.5 eggs in 2.7 ± 1.2 egg masses, and \u0000there was a reduction of 51% in the average number of hatched snails (11.5 ± 7.5) in comparison to the control \u0000group (23.9 ± 18.0) for four weeks (p = 0.003). The results indicate that the lethal concentration (LC50) of \u0000the 0.012% aqueous solution of original Roundup has a significant action on Biomphalaria glabrata for up to \u000027 days. Roundup significantly diminished the fertility of infected and exposed snails in comparison with the \u0000control group.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43700559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Análise comparativa de protocolos para extração de DNA genômico em Prosopis juliflora (Sw.) DC 细叶Prosopis juliflora（Sw.）DC基因组DNA提取方案的比较分析

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E76552

L. H. Santos, Cibelle Santos Dias, Jardyelle Carvalho Lima, Messulan Rodrigues Meira, C. B. M. Cerqueira-Silva

{"title":"Análise comparativa de protocolos para extração de DNA genômico em Prosopis juliflora (Sw.) DC","authors":"L. H. Santos, Cibelle Santos Dias, Jardyelle Carvalho Lima, Messulan Rodrigues Meira, C. B. M. Cerqueira-Silva","doi":"10.5007/2175-7925.2021.E76552","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E76552","url":null,"abstract":"The objective of the present research was to compare the efficiency among seven protocols adapted for the extraction of genomic DNA from the species Prosopis juliflora (Sw.) DC. The protocols used were based on their buffers: SDS 10%, CTAB 5%, Sorbitol, CTAB 3% and Sorbitol, SDS 0.7% and NaCl, CTAB 2%, CTAB 2% and NaCl 1.4M. In all tests, the use of β-mercaptoethanol, liquid nitrogen, proteinase K and RNAses was eliminated. The samples consisted of leaf primordia in each specimen in triplicate. The quantity and quality of the extracted DNA were evaluated by electrophoresis in 1% agarose gel and by reading the absorbances in a spectrophotometer. For amplification reactions, the ISSR primer was used. Among the tested extraction buffers, SDS 10%, CTAB 5% and CTAB 3%, and Sorbitol were the most efficient for P. juliflora. Of these three protocols, CTAB 3% and Sorbitol showed a better level of purity despite the lower amount of DNA (123 ng/µL) compared to 10% SDS and 5% CTAB buffers (312 and 321 ng/µL, respectively, and high content of contaminants). We conclude that it is possible to use extraction protocols without additives that are harmful to human health and at a lower cost in the extraction process.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44240115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diversidade das pescarias realizadas com rede de emalhar em um estuário tropical do nordeste do Brasil 巴西东北部热带河口刺网渔业的多样性

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77545

Severino Adriano de Oliveira Lima, H. A. Andrade, Marcondes Agostinho Gonzaga Junior, Donovan Filipe Henrique Pinto

{"title":"Diversidade das pescarias realizadas com rede de emalhar em um estuário tropical do nordeste do Brasil","authors":"Severino Adriano de Oliveira Lima, H. A. Andrade, Marcondes Agostinho Gonzaga Junior, Donovan Filipe Henrique Pinto","doi":"10.5007/2175-7925.2021.E77545","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E77545","url":null,"abstract":"The aim of the present research was to study the diversity of the ichthyofauna captured with the most commonly used gillnet in the state of Pernambuco. In total, 14 collections were made in the Santa Cruz Canal (CSC) between July 2013 and June 2014. The samples were collected using a 30 mm mesh gillnet. The captured fish were removed and separated in margin and center positions, as well as in the net’s vertical position (upper, middle, and lower). Concerning frequency of occurrence, the species were classified as common, accessory, or occasional. Alpha and beta diversity analyses were performed. In total, 19 species were captured, with Opisthonema oglinum and Anchovia clupeoides being the only constants. The estimated alpha diversities did not show significant differences in the temporal and spatial strata. In the beta diversity, two groups were distinguished, with the largest generally occurring in large abundances of O. oglinum. The evaluated net was efficient for the target specie O. oglinum, while the catches of other species corresponded to less than 13% of the icitiofauna already found in the CSC. Therefore, management measures that aim to restrict the use of fishing gear for the protection of diversity considering the seasonality and spatiality in the channel is not recommended.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45813550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vascular epiphytic component of “Brejo de altitude” in northeastern Brazil: floristic composition and phytosociological structure 巴西东北部“布雷霍高原”的维管附生成分:区系组成和植物社会学结构

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E76492

R. G. Dias-Terceiro, V. S. Gomes, M. Menezes, L. O. Demarchi, J. Fabricante, M. B. Albuquerque

引用次数: 1

A survey of Calliphoridae and Mesembrinellidae (Diptera) in semideciduous seasonal forest, Paraná, Brazil 巴西巴拉那半熟季节性森林中丽蝇科和夜蛾科的调查

Biotemas Pub Date : 2021-03-04 DOI: 10.5007/2175-7925.2021.E77073

Alex Sandro Barros de Souza, Marcos Magalhães de Souza, Patrícia Pereira Gomes, Jean Victor Nery da Silva, Giuliano Kaulfuss Bertinoti, José Adolfo Mota de Almeida

{"title":"A survey of Calliphoridae and Mesembrinellidae (Diptera) in semideciduous seasonal forest, Paraná, Brazil","authors":"Alex Sandro Barros de Souza, Marcos Magalhães de Souza, Patrícia Pereira Gomes, Jean Victor Nery da Silva, Giuliano Kaulfuss Bertinoti, José Adolfo Mota de Almeida","doi":"10.5007/2175-7925.2021.E77073","DOIUrl":"https://doi.org/10.5007/2175-7925.2021.E77073","url":null,"abstract":"Calliphoridae and Mesembrinellidae include Diptera species that are good indicators of changes in forest environments. The present work aimed to inventory the Calliphoridae and Mesembrinellidae in Ilha Grande National Park and correlate the abundance of these organisms with environmental variables. Adult dipterans were collected during two expeditions in 2019 using two sampling methods, a Malaise trap and baited traps, distributed on two islands in the park. During the study, 1,007 individuals of 12 species of Calliphoridae and four species of Mesembrinellidae were collected. Family Calliphoridae was more abundant (97.12%). The abundance of native species showed a strong negative correlation with the abundance of the genus Chrysomya. Rodrigues Island (Island I) had higher diversity levels and no species of Chrysomya were recorded in this area, which may indicate a greater degree of environmental preservation. The results indicate that human action in natural environments can negatively impact species diversity, as found in Ilha Grande National Park that, historically, was partially occupied and is still in the process of regeneration.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45547807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Avaliação da inflamabilidade de espécies nativas do cerrado 塞拉多本地物种可燃性评价

Biotemas Pub Date : 2020-12-09 DOI: 10.5007/2175-7925.2020.e74323

Marcileia Dias de Oliveira, Josué Luiz Marinho Junior, Ângela Helena Silva Mendes Stival, Bárbara Gomes Ferreira, Francisca de Cássia Silva da Silva, Marcos Vinicius Giongo Alves

{"title":"Avaliação da inflamabilidade de espécies nativas do cerrado","authors":"Marcileia Dias de Oliveira, Josué Luiz Marinho Junior, Ângela Helena Silva Mendes Stival, Bárbara Gomes Ferreira, Francisca de Cássia Silva da Silva, Marcos Vinicius Giongo Alves","doi":"10.5007/2175-7925.2020.e74323","DOIUrl":"https://doi.org/10.5007/2175-7925.2020.e74323","url":null,"abstract":"Na ocorrencia de incendios florestais, a propagacao se deve principalmente as caracteristicas das folhas. De certa forma, as folhas funcionam como potencializador ou retardador da propagacao de incendios, ja que as que possuem baixa inflamabilidade podem servir como barreiras de protecao contra incendios. O presente trabalho teve por finalidade, analisar a inflamabilidade de 6 especies do cerrado. Para a realizacao do experimento, foi utilizado o epirradiador com uma faixa de temperatura controlada entre 630 a 650 oC. Das especies avaliadas foram coletadas 50 amostras de 1g (± 0,1 g) de cada especie. Foram realizadas 50 repeticoes de queima, na qual se pode analisar o Tempo para Ignicao (TI), Frequencia de ignicao (FI), Duracao de Combustao (DC), Indice de Combustao (IC) e Valor de Inflamabilidade (VI). Os valores de inflamabilidade das especies foram: Protium Heptaphyllum igual a 1 (fracamente inflamavel); Caryocar brasiliense Cambess., Byrsonima verbascifolia (L.) DC., Acacia polyphylla e Terminalia argentea Mart. iguais a 3 (inflamaveis); e a especie Lafoensia pacari igual a 4 (altamente inflamavel). Percebeu-se que o teor de umidade das especies nao teve correlacao com os valores de inflamabilidade e que outros fatores, como presenca de oleos, resinas e gomas vegetais podem influenciar no processo de combustao.","PeriodicalId":29999,"journal":{"name":"Biotemas","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45501604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0