Minerva Biotechnology and Biomolecular Research最新文献

{"title":"Safety and efficacy of injection of exosomes derived from human umbilical cord mesenchymal stem cells in the treatment of limb ischemia: an in-vivo evaluation in mice","authors":"T. Le, N. Vu, P. Nguyen, P. D. Huynh, Phuc Van Pham","doi":"10.23736/s2724-542x.22.02936-4","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02936-4","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91118948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Daturalactones as immunomodulators: activation of immune cells conferring cytotoxicity towards colon and pancreatic cancer cells","authors":"G. Chandan, Chetan Kumar, N. Satti, H. Tuli, S. Fagoonee, S. Haque, A. Saini, R. Saini","doi":"10.23736/s2724-542x.22.02931-5","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02931-5","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85986147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytokine profile in patients with osteoarthritis after SARS-CoV-2 infection","authors":"D. Krenytska, L. Kot, T. Halenova, N. Raksha, T. Vovk, O. Savchuk, R. Pellicano, L. Abenavoli, T. Falalyeyeva, L. Ostapchenko","doi":"10.23736/s2724-542x.22.02943-1","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02943-1","url":null,"abstract":"BACKGROUND: Severe acute respiratory syndrome-related Coronavirus 2 (SARS-CoV-2) іnfection induces a pro-inflammatory state of an organism with long-term systemic consequences as a result. Systemic inflammation, characterized by a high circulating level of inflammatory cytokines, is a significant factor influencing articular cartilage metabolism in osteoarthritis (OA). This study aimed to determine the levels of pro-inflammatory and anti-inflammatory cytokines in plasma of patients with OA following SARS-CoV-2 infection and to compare them with those of healthy controls. METHODS: The experiment involved patients of the Orthopedic Specialty Clinic aged 46 to 69 diagnosed with knee OA. Among persons with joint pathology a group of convalescent patients from 6-9 months after COVID-19 was identified. The control group involved relatively healthy donors. The plasma levels of pro-inflammatory (IL-1β, IL-6, IL-8, IL-12β, tumor necrosis factor α [TNF-α], interferon-gamma [IFN-γ]) and anti-inflammatory (IL-4 and IL-10) cytokines were determined by enzyme-linked immunosorbent assay. RESULTS: It was established that in patients with OA, as well as after suffering from SARS-CoV-2 infection, an increase in the plasma levels of IL-1β was observed against the background of a decrease in the levels of IL-4, IL-8, IL-10, IL- 12β, TNF-α and IFN-γ, compared to the healthy controls. COVID-19 more significantly influenced the plasma levels of pro-inflammatory cytokines IL-1β and IL-12β. CONCLUSIONS: The results indicate the imbalance of pro- and anti-inflammatory cytokines in the plasma in patients with OA for a long post- COVID. Сhanges in the levels of inflammatory mediators suggest distinct immunoregulatory mechanisms involved in the pathogenesis of both joint pathology and systemic disorders caused by SARS-CoV-2. [ FROM AUTHOR]","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75781415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuroprotective effect of curcumin and curcumin-integrated nanocarriers in stroke: from mechanisms to therapeutic opportunities","authors":"J. Sharifi‐Rad, Z. Almarhoon, C. Adetunji, Olugbenga SAMUEL MICHAEL, Deepak Chandran, R. Radha, N. Sharma, M. Kumar, D. Calina","doi":"10.23736/s2724-542x.22.02946-7","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02946-7","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87826371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of inherent bacterial DNA contamination in a qPCR master mix: concerns about background DNA of reagents","authors":"Luciano A. Palomino-Kobayashi, M. J. Pons, J. Ruiz","doi":"10.23736/s2724-542x.22.02925-x","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02925-x","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85489435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and screening of alpha-galactosidase-producing probiotics with anti-flatulence potential","authors":"Aboozar Kazemi, S. Hemmati, Mohammad Hossein Morowvat, A. Gholami, Y. Ghasemi","doi":"10.23736/s2724-542x.21.02866-2","DOIUrl":"https://doi.org/10.23736/s2724-542x.21.02866-2","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85000029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Non-O157 Escherichia coli serotypes isolated from the stool of under five years old children presenting with diarrhea in Lagos, Nigeria","authors":"T. Jolaiya, S. Braun, A. Ajayi, A. Coker, R. Ehricht, S. Monecke, R. Pellicano, S. Smith","doi":"10.23736/s2724-542x.22.02905-4","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02905-4","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80285327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systematic structure guided clustering of chemical lead compounds targeting RdRp of SARS-CoV-2","authors":"A. Alsulimani, T. Bhardwaj, E. Janahi, Atiah H. Almalki, B. N. Tewari, M. Wahid, M. Alkhanani, P. Somvanshi, S. Haque","doi":"10.23736/s2724-542x.22.02869-3","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02869-3","url":null,"abstract":"BACKGROUND: To combat the global health issue caused by SARS-CoV2, scientists are attempting various therapeutic approaches towards drug discovery including computational biology and drug-repurposing. Recent studies have highlighted the conserved nature of RNA-dependent RNA polymerase (RdRp) of coronaviruses affecting human, bat and animals. In this study attempts have been made to identify the potential inhibitors of RdRp by utilizing molecular docking and MD simulation studies. METHODS: Systematic structure-based screening of chemical compounds from public libraries was performed to identify the potential lead molecules inhibiting RdRp. This structure driven clustering of compounds is based on decision tree model generated by combining two properties: 1) shape descriptors;and 2) critical number of multiple bonds. The enabled screening of potential chemical compounds was subjected to molecular docking followed by molecular dynamics simulation studies. RESULTS: The results revealed that the stability of protein-drug complex structure was in the order of RdRp-Oxoglaucine >RdRp-Flutroline >RdRp-Brucine complex. CONCLUSIONS: This study identifies Oxoglaucine, Brucine and Flutroline as prospective inhibiting agents of SARS-CoV-2 RdRp and further warrants for experimental validation. ( [ FROM AUTHOR] Copyright of Minerva Biotechnology & Biomolecular is the property of Edizioni Minerva Medica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86289173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-silico analysis of multiepitope based vaccine targeting respiratory viruses SARS, MERS and SARS-CoV-2","authors":"A. Sen, Ritu Bansal, Sanika Mohagaonkar, T. Bhardwaj, B. Rathi, Atiah H. Almalki, E. Janahi, A. Alsulimani, B. N. Tewari, P. Somvanshi, S. Haque","doi":"10.23736/s2724-542x.22.02868-1","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02868-1","url":null,"abstract":"BACKGROUND: Recurrent outbreaks of respiratory viruses like SARS-CoV (severe acute respiratory syndrome-coronavirus, 2002), MERS (Middle East respiratory syndrome, 2012) including the ongoing SARS-CoV-2 (2019) pandemic warrants for a single-broad-spectrum vaccine against these respiratory viruses. METHODS: In the present study, phylogenetic analysis followed by in-silico identification of vaccine candidates for SARS, MERS and SARS- CoV-2 was performed by exploiting T-cell and B-cell mapping to ascertain the best possible epitopes for effector humoral- and cell-mediated immune response. Further, population-coverage analysis of the identified epitopes followed by the designing of chimera of epitope-based vaccine was done using linkers and adjuvants. Docking study was done to appraise the interaction of the proposed vaccine with ACE2 (angiotensin converting enzyme-2) receptor (SARS and SARS-CoV-2) and HLA-B7 (human leukocyte antigen) receptor (MERS). The stability of the vaccine chimera was confirmed by molecular dynamics performed for 20 ns;this was followed by codon optimization and in-silico cloning. RESULTS: Phylogenetic analysis revealed similarity among SARS-CoV-2, SARS-CoV and bat SARS-like coronavirus. Both, SARS-CoV and SARS-CoV-2 were from different class than MERS, whereas SARS-CoV-2 showed more relatedness with Bat SARS-like coronaviruses. The most suitable epitopes found were LSFELLNAPATVCGP (SARS), LVTLAILTALRLCAY (SARS-CoV-2) and YTSAFNWLL (MERS) with nearly 98% population coverage. Molecular docking followed by simulation studies revealed high number of hydrogen bonds, stable RMSD values and acceptable RMSF flexibility scores, indicating stable interactions of the vaccine with ACE2 and MHC receptors (Major histocompat-ibility complex). Expression of the designed multiepitope vaccine in E. coli (Escherichia coli) expression system was confirmed by in-silico cloning/codon optimization. CONCLUSIONS: Further, in-vitro and in-vivo experimental validation studies are required to endorse our current findings. [ FROM AUTHOR] Copyright of Minerva Biotechnology & Biomolecular is the property of Edizioni Minerva Medica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full . (Copyright applies to all s.)","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79039377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma matrix metalloproteinases (MMP-2 and MMP-9) as prognostic biomarkers in coronary heart disease","authors":"T. Marynenko, T. Halenova, N. Raksha, T. Vovk, Y. Tyravska, O. Savchuk, T. Falalyeyeva, S. Fagoonee, L. Abenavoli, L. Ostapchenko","doi":"10.23736/s2724-542x.22.02906-6","DOIUrl":"https://doi.org/10.23736/s2724-542x.22.02906-6","url":null,"abstract":"","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82293957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}