Minerva Biotechnology and Biomolecular Research最新文献

miR-141-5p regulates cervical cancer malignant progression by targeting MRVI1 miR-141-5p通过靶向MRVI1调控宫颈癌恶性进展

4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2026-02-01 DOI: 10.23736/s2724-542x.25.03244-4

Li Jiang, Chiwen Liu, Xinlan Xu, Yinling Zhao, Donglan Yuan

{"title":"miR-141-5p regulates cervical cancer malignant progression by targeting MRVI1","authors":"Li Jiang, Chiwen Liu, Xinlan Xu, Yinling Zhao, Donglan Yuan","doi":"10.23736/s2724-542x.25.03244-4","DOIUrl":"https://doi.org/10.23736/s2724-542x.25.03244-4","url":null,"abstract":"BACKGROUND: Cervical cancer (CC) is caused by human papillomavirus (HPV) infection and has serious impacts on women’s health. While some HPV vaccines are already available, there is still a need for deeper understanding of the pathogenesis of CC and better ways to diagnose and treat it.METHODS: qRT-PCR was used to determine the level of miR-141-5p expression in CC. A CCK-8 assay, colony formation experiment, and Transwell assay were used to determine the influence of miR-141-5p on biological characteristics. A dual luciferase assay was employed to examine the correlation between miR-141-5p and MRVI1. The expression of MRVI1 and proteins linked to epithelial-mesenchymal transition (EMT) were identified by western blot. Knockdown of MRVI1 was performed to investigate its revertive effect on miR-141-5p down-regulation. A subcutaneous tumor-graft model was constructed to explore the impacts of down-regulating miR-141-5p on tumors in vivo, and the positivity of Ki-67 and MRVI1 in CC tissues was detected by immunohistochemistry.RESULTS: The level of miR-141-5p was markedly increased in CC. miR-141-5p knockdown reduced the viability, migration, and invasion of CC cells and hindered their EMT process. The opposite result was obtained when miR-141-5p was over-expressed. MRVI1 was screened as a target gene of miR-141-5p. MiR-141-5p negatively regulated MRVI1 expression, and silencing MRVI1 reversed the impact of miR-141-5p down-regulation on CC cells. Knockdown of miR-141-5p upregulated MRVI1 expression in tumor tissues and inhibited CC formation in vivo.CONCLUSIONS: miR-141-5p was markedly increased in CC and regulated CC malignant progression by targeting and negatively regulating MRVI1. Additionally, knockdown of miR-141-5p inhibited CC progression in vivo.","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":"37 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-767-5p negatively regulates HIF3A to regulate proliferation and EMT in gastric cancer through the PI3K/AKT pathway miR-767-5p负调控HIF3A，通过PI3K/AKT通路调控胃癌的增殖和EMT

4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2025-09-01 DOI: 10.23736/s2724-542x.25.03258-4

Xian-Hua Lin, Yunyun LIN

{"title":"miR-767-5p negatively regulates HIF3A to regulate proliferation and EMT in gastric cancer through the PI3K/AKT pathway","authors":"Xian-Hua Lin, Yunyun LIN","doi":"10.23736/s2724-542x.25.03258-4","DOIUrl":"https://doi.org/10.23736/s2724-542x.25.03258-4","url":null,"abstract":"BACKGROUND: Gastric cancer (GC) is commonly found among gastrointestinal tract cancers, and it often exhibits a poor prognosis. MiRNA-767-5p, a microRNA (miRNA), has been related to the progression and prognosis of numerous human cancers. Currently, the level of miR-767-5p expression and its function have not been thoroughly investigated in GC.METHODS: We examined miR-767-5p and target gene hypoxia-inducible factor-3α (HIF3A) mRNA expression in GC and normal tissues, and also in GES-1 cells, N87 cells, and GAS cells using qRT-PCR. We used MTT, plate cloning, and EdU staining assays to measure the variations in the biological properties of GC cells. The Epithelial-mesenchymal transition (EMT) process and the effects of miR-767-5p and HIF3A on the PI3K/AKT signaling pathway in GC cells were analyzed through Western blotting.RESULTS: MiR-767-5p exhibited a high level of expression in GC. Inhibitors of miR-767-5p hindered the proliferation, clonal formation capability, and EMT process in GC cells. In addition, there was a negative correlation between miR-767-5p and HIF3A mRNA expression. The silencing of HIF3A boosted proliferation and colony formation abilities in GC cells, and partially offset the suppression inflicted by miR-767-5p inhibitors on these cells. Silencing miR-767-5p impeded the PI3K/AKT pathway, whereas silencing HIF3A activated this pathway. The use of PI3K/AKT pathway agonists amplified the effects of HIF3A silencing, which could further enhance GC cell proliferation and EMT, as opposed to the effects of PI3K/AKT pathway inhibitors.CONCLUSIONS: MiR-767-5p negatively regulates HIF3A and is involved in the proliferation and EMT process via the PI3K/AKT signaling pathway in GC.","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":"37 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum exosome miR-208b-3p worsens cerebral microvascular endothelial cell injury by inhibiting NLK expression 血清外泌体miR-208b-3p通过抑制NLK表达加重脑微血管内皮细胞损伤

4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2025-09-01 DOI: 10.23736/s2724-542x.25.03257-2

Chongli GAO, Long Yi

{"title":"Serum exosome miR-208b-3p worsens cerebral microvascular endothelial cell injury by inhibiting NLK expression","authors":"Chongli GAO, Long Yi","doi":"10.23736/s2724-542x.25.03257-2","DOIUrl":"https://doi.org/10.23736/s2724-542x.25.03257-2","url":null,"abstract":"BACKGROUND: Traumatic brain injury (TBI) can cause pathological disruption of the blood-brain barrier, leading to neurological damage. Recent studies suggest that microRNA-208b-3p (miR-208b-3p) in serum exosomes may play a role in TBI, but its precise mechanism remains unclear. This study aims to investigate the effect of serum exosomal miR-208b-3p on brain microvascular endothelial cells (BMVECs) and its regulatory mechanism involving Nemo-like kinase (NLK).METHODS: Serum exosomes were extracted from patients with TBI and co-cultured with BMVECs following scratch injury. The expression levels of tight junction proteins, inflammatory factors, and NLK in BMVECs were measured using Western blotting and quantitative real-time PCR (QRT-PCR). The levels of miR-208b-3p in exosomes were also assessed. Bioinformatics analysis and a dual luciferase assay were used to confirm the targeting relationship between miR-208b-3p and NLK.RESULTS: Serum exosomes from TBI patients were found to be rich in miR-208b-3p. miR-208b-3p was shown to regulate apoptosis in BMVECs and directly target NLK, leading to its reduced expression following scratch injury. Downregulation of NLK was associated with increased endothelial cell damage and impaired nerve repair.CONCLUSIONS: Serum exosomal miR-208b-3p exacerbates traumatic injury by downregulating NLK expression in BMVECs, thereby aggravating blood-brain barrier disruption and impeding neural repair. These findings provide a potential therapeutic target for the treatment of traumatic brain injuries.","PeriodicalId":29824,"journal":{"name":"Minerva Biotechnology and Biomolecular Research","volume":"37 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nanoparticles therapy: inspiration from the mechanism by which SARS-CoV-2 disrupts autophagy 纳米粒子疗法：SARS-CoV-2 破坏自噬机制的启示

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.03013-4

Zhaojunli Wang, Qi Wang, Jiancheng Ji, Yuanyuan Qian

引用次数: 0

Actin-binding proteins: insight into the role of plastin 1 in pancreatic cancer 肌动蛋白结合蛋白：深入了解塑蛋白 1 在胰腺癌中的作用

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.03068-7

S. Fagoonee, Javad Sharifi‐Rad

引用次数: 0

Regulation of plastin 1 expression modulates pancreatic carcinoma progression by affecting the glycolytic signaling pathway 通过影响糖酵解信号通路调控塑形素 1 的表达，从而调节胰腺癌的进程

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.03010-9

Aitao Sun, Jiancheng Liu, Fei Yang, Guangzhu Su

引用次数: 0

Alvaradoin E: an update of its anticancer potential and mechanisms of action Alvaradoin E：其抗癌潜力和作用机制的最新情况

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.03035-3

D. Kitić, Bojana Miladinović, Milica Randjelović, S. Fagoonee, Dragos Popa, D. Calina, Javad Sharifi‐Rad

引用次数: 0

Therapeutic applications of CRISPR-Cas9 in diabetes mellitus: a perspective review CRISPR-Cas9 在糖尿病中的治疗应用：透视综述

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.02996-6

J. Bora, Sumira Malik, Shristi Kishore, Sarvesh Rustagi, Faraz Ahmad, S. Fagoonee, R. Pellicano, Shafiul Haque

引用次数: 0

Phage therapy to combat mycobacterial infections: a therapeutic perspective 噬菌体疗法抗击分枝杆菌感染：治疗视角

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.03019-5

Saurabh Gupta, Ayushi Sharma, Rasanpreet Kaur, A. Goel, S. Vir Singh

引用次数: 0

Effects of a novel combination of magnesium, tryptophan, resveratrol, and saffron on oxidative stress, prostaglandin F2α, and intracellular Ca2+ levels in an in-vitro model of myometrium 镁、色氨酸、白藜芦醇和藏红花的新组合对子宫肌瘤体外模型中氧化应激、前列腺素 F2α 和细胞内 Ca2+ 水平的影响

IF 1 4区生物学

Minerva Biotechnology and Biomolecular Research Pub Date : 2024-01-01 DOI: 10.23736/s2724-542x.23.02989-9

Stefania Battaglia, Marco Cozzi, Francesca R. Ranieri, Elisa Gaio, Federico Benetti, Valeria Curti

引用次数: 0