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Different Responses of Proliferating and Resting Tumor HeLa Cells to Pulsed High Repetition Rate Low-Intensity Laser Light at 271 nm 271 nm脉冲高重复率低强度激光对增殖和静止肿瘤HeLa细胞的不同反应
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/LC.1.153
T. Karu, G. Kalendo, V. Letokhov, V. Lobko
{"title":"Different Responses of Proliferating and Resting Tumor HeLa Cells to Pulsed High Repetition Rate Low-Intensity Laser Light at 271 nm","authors":"T. Karu, G. Kalendo, V. Letokhov, V. Lobko","doi":"10.1155/LC.1.153","DOIUrl":"https://doi.org/10.1155/LC.1.153","url":null,"abstract":"The action of pulsed high repetition rate UV laser radiation (2-nd harmonic of Cu vapor \u0000laser, λ = 271 nm, pulse duration 18 nsec, repetition rate 10 kHz) on proliferating and \u0000resting HeLa cells has been studied. The intensity of DNA synthesis changes after such kinds \u0000of irradiation for both types of cells. For proliferating cells DNA synthesis is suppressed, \u0000while for the resting ones stimulation is observed in the dose range from 0.05 to 5 J/m2.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134271839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
TEMPERATURE INDUCED PROTEIN UNFOLDING AND FOLDING OF RNase A STUDIED BY TIME-RESOLVED INFRARED SPECTROSCOPY 用时间分辨红外光谱研究了温度诱导的rna酶的蛋白质展开和折叠
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1999/28202
H. Georg, C. Wharton, F. Siebert
{"title":"TEMPERATURE INDUCED PROTEIN UNFOLDING AND FOLDING OF RNase A STUDIED BY TIME-RESOLVED INFRARED SPECTROSCOPY","authors":"H. Georg, C. Wharton, F. Siebert","doi":"10.1155/1999/28202","DOIUrl":"https://doi.org/10.1155/1999/28202","url":null,"abstract":"When a protein finds its native three-dimensional structure from the unstructured amino-acid chain various processes spanning a large time range are relevant. To understand the mechanism of protein folding one needs to cover the entire folding/ refolding (U↔N) reaction on a structural level. In the case of RNase A, the main structural changes occur in the ms time range, that can be monitored with rapid-scan- FTIR spectroscopy combined with rapid mixing techniques. To induce unfolding we inject aqueous protein solution into a hot IR cuvette and record the time course of the spectral changes. A lag phase is found when the unfolding conditions are relatively weak, suggesting an unfolding intermediate.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134003576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Temporal Characterization of a Plasma Produced by Interaction of Laser Pulses with Water Solutions 激光脉冲与水溶液相互作用产生等离子体的时间表征
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1080/02786270215155
J. Ahmed, N. Terzi, Z. B. Lakhdar, G. Taieb
{"title":"Temporal Characterization of a Plasma Produced by Interaction of Laser Pulses with Water Solutions","authors":"J. Ahmed, N. Terzi, Z. B. Lakhdar, G. Taieb","doi":"10.1080/02786270215155","DOIUrl":"https://doi.org/10.1080/02786270215155","url":null,"abstract":"The temporal evolution of a plasma formed by the interaction of a Nd-YAG 10 ns laser pulse with the surface of aqueous solutions of CaCl 2 , has been observed by analyzing the variation of the emission spectra of ions and neutral atoms in the time interval after the laser pulse, 500 ns T e using a Saha-Boltzmann analysis. The broadening of the resonance line of CaI at 4227 A was used to deduce the electronic density N e , with the aid of the semiclassical theory of Stark broadening. A value of T e ≈ 28000 K decreasing slightly to 21000 K, and an exponential decay for N e were found, with N e ≈ 1:25 × 10 18 cm -3 at 500 ns and τ e = ( 1200 ± 50 ) ns.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134341586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Quantum Beats in Transient Spectroscopy of J-Aggregates j聚集体瞬态光谱中的量子拍
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1996/63828
H. Glaeske, K. Feller, E. Gaiaukas, L. Knöll
{"title":"Quantum Beats in Transient Spectroscopy of J-Aggregates","authors":"H. Glaeske, K. Feller, E. Gaiaukas, L. Knöll","doi":"10.1155/1996/63828","DOIUrl":"https://doi.org/10.1155/1996/63828","url":null,"abstract":"Quantum beats in transient absorption of PIC-J-aggregates, modelled by a modified 3-level-system are calculated. Experimental conditions for the possibility of their observation are investigated. The influence of the temperature dependent phase relaxation on the temporal evolution of quantum beat signals is considered.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132965008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Angular selection of products by use of saturated-absorption techniques in the Cs(7P)+H2→CsH+H reaction Cs(7P)+H2→CsH+H反应中饱和吸收技术的角度选择
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1990/79301
J. l’Hermite, G. Rahmat, R. Vetter
{"title":"Angular selection of products by use of saturated-absorption techniques in the Cs(7P)+H2→CsH+H reaction","authors":"J. l’Hermite, G. Rahmat, R. Vetter","doi":"10.1155/1990/79301","DOIUrl":"https://doi.org/10.1155/1990/79301","url":null,"abstract":"The Cs( 7 P)+H 2 → CsH + H ​ reaction is studied in a crossed-beam experiment with laser-induced \u0000fluorescence detection of CsH products. The usual flux ( ≈ 50 mW / mm 2 ) which is delivered by the C.W. \u0000tunable dye laser used in the experiment is enough to saturate the absorption by CsH products. Then, by \u0000crossing twice the laser beam through the collision volume (counterpropagating beams), one realizes the \u0000conditions of saturated-absorption experiments: when the laser frequency is tuned to a resonance \u0000frequency of CsH products, a defect to absorption occurs for these products which scatter in the collision \u0000plane and a \"saturation dip\" appears at the center of the corresponding fluorescence profile. Application \u0000of this technique to crossed-beam experiments can lead to the selection of product molecules which scatter \u0000in any definite plane. A different geometry of the laser beams (bent beams) is proposed to select molecules \u0000which scatter in any definite direction: it could be applied to detect an asymmetry in the scattering of the \u0000products with respect to the collision axis, when a particular preparation of the reagents is realized.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"153 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114626462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Bridged Quaterphenyls as Flashlamp-Pumpable Laser Dyes 桥接季苯基作为闪光灯可泵送激光染料
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/LC.7.343
J. Kauffman, C. Kelley, Alem Ghiorghis, E. Neister, Lorne Armstrong, Paul R. Prause
{"title":"Bridged Quaterphenyls as Flashlamp-Pumpable Laser Dyes","authors":"J. Kauffman, C. Kelley, Alem Ghiorghis, E. Neister, Lorne Armstrong, Paul R. Prause","doi":"10.1155/LC.7.343","DOIUrl":"https://doi.org/10.1155/LC.7.343","url":null,"abstract":"Partial bridging of the o,o′ positions of the aromatic rings in quaterphenyl gave superior \u0000flashlamp-pumpable laser dyes. Alkyl-substituted carbon bridges gave superior dyes to \u0000those containing oxygen or alkyl-substituted nitrogen or silicon bridges. Dyes lasing in \u0000the 365–390 nm region with improved energy output, lifetime and solubility were \u0000discovered.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"151 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114907205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Laser Spectroscopic Investigation of High Rydberg States of no:Decay Dynamics Near the First Ionization Threshold 第一电离阈值附近高里德伯态的激光光谱研究
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1994/49739
A. Fujii, N. Morita
{"title":"Laser Spectroscopic Investigation of High Rydberg States of no:Decay Dynamics Near the First Ionization Threshold","authors":"A. Fujii, N. Morita","doi":"10.1155/1994/49739","DOIUrl":"https://doi.org/10.1155/1994/49739","url":null,"abstract":"With a state-selective laser excitation, two main decay processes, autoionization and predissociation, in vibrational superexcited Rydberg states (n = 8–12, l = s, p, and f, v = 1) of NO have been studied, directly detecting not only NO","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114951896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 14
Picosecond Studies of Intramolecular Double Proton Transfer by Excite- and Probe-Technique 分子内双质子转移的皮秒激和探针研究
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/LC.8.377
M. Kaschke, S. Rentsch, J. Opfermann
{"title":"Picosecond Studies of Intramolecular Double Proton Transfer by Excite- and Probe-Technique","authors":"M. Kaschke, S. Rentsch, J. Opfermann","doi":"10.1155/LC.8.377","DOIUrl":"https://doi.org/10.1155/LC.8.377","url":null,"abstract":"The double proton transfer of 2,2'-bipyridyl-3,3'-diol (BP(OH)2) in isooctane after UV excitation has been studied by excite and probe beam spectroscopy with a time resolution of 5 ps. Estimates for the excited state proton transfer rate as well as for the extinction coefficients of the excited state absorption bands of the primarily excited species and of the tautomer have been determined.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"169 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115044427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 10
SINGLE PHOTON IONISATION MASS SPECTROMETRY USING LASER-GENERATED VACUUM ULTRAVIOLET PHOTONS 使用激光产生的真空紫外光子的单光子电离质谱法
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1997/53174
N. Lockyer, J. Vickerman
{"title":"SINGLE PHOTON IONISATION MASS SPECTROMETRY USING LASER-GENERATED VACUUM ULTRAVIOLET PHOTONS","authors":"N. Lockyer, J. Vickerman","doi":"10.1155/1997/53174","DOIUrl":"https://doi.org/10.1155/1997/53174","url":null,"abstract":"This paper provides an overview of the method of single photon ionisation mass \u0000spectrometry. A review of the theory of frequency upconversion using third-order 4- \u0000wave sum mixing in isotropic media and experimental results of third harmonic \u0000generation (THG) using a frequency tripled 355 nm Nd:YAG pump source are \u0000presented. Vacuum Ultra-violet (VUV) photons of wavelength 118 nm are detected in an \u0000acetone ionisation chamber. The emphasis of this paper is on the practical aspects of \u0000generating and detecting the VUV photons and using them for single photon ionisation \u0000(SPI) in the ion source of a mass spectrometer. Optimum gas pressures for THG in Xe \u0000and Xe/Ar mixtures are established. For a pump beam of well defined mode structure the \u0000optimum gas pressures are in excellent agreement with theory. The major loss \u0000mechanism is attributed to re-absorption of VUV by the tripling gas. SPI mass spectra \u0000of hexane and the biomolecule valyl-valine are presented illustrating the power of the \u0000technique.","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116018552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 47
Triplet 1-nitronaphthalene and competitive energy and electron transfer reactions with trans-stilbene 三态1-硝基萘与反式二苯乙烯的竞争性能量和电子转移反应
Laser Chemistry Pub Date : 1900-01-01 DOI: 10.1155/1999/92415
T. Fournier, G. Scholes, I. Gould, S. Tavender, D. Phillips, A. Parker
{"title":"Triplet 1-nitronaphthalene and competitive energy and electron transfer reactions with trans-stilbene","authors":"T. Fournier, G. Scholes, I. Gould, S. Tavender, D. Phillips, A. Parker","doi":"10.1155/1999/92415","DOIUrl":"https://doi.org/10.1155/1999/92415","url":null,"abstract":"A time resolved resonance Raman (TR3) study of 1-nitronaphthalene (1NN) first triplet excited state in various solvents is combined with ab initio calculations to determine the nature and the geometry of this state in environments of different polarity. A transient absorption and TR3 study of the reactions of triplet 1NN with trans-stilbene (tS) in solution in both non-polar and polar solvents is reported. In polar solution, 31NN acts as an electron acceptor whereas in non-polar solution only energy transfer to tS is observed. Moreover, at high concentrations of tS, the radical cation tS","PeriodicalId":296295,"journal":{"name":"Laser Chemistry","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116353672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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