D. R. Husain, R. Wardhani, Fiqha Septia Ningsih, Fuad Gani
{"title":"Identification of Probiotic Bacteria Isolated from Domestic Chickens (Gallus domesticus) Using the 16S rRNA Gene Method","authors":"D. R. Husain, R. Wardhani, Fiqha Septia Ningsih, Fuad Gani","doi":"10.36380/jwpr.2023.4","DOIUrl":"https://doi.org/10.36380/jwpr.2023.4","url":null,"abstract":"The intestines of domestic poultry (Gallus domesticus) are one of the potential sources of probiotic bacteria that can produce antibacterial agents. The objective of this study was to identify the types of probiotic bacteria obtained from the digestion of domestic poultry using the molecular analysis method of 16S rRNA gene sequencing. Observations were conducted on colony morphology, gram staining, biochemical tests, and antibacterial activity using the diffusion agar method. Molecular analysis of DNA extraction was carried out, followed by the amplification of samples using a 16S rRNA universal primer. Dielectrophoresis and sequencing were performed on the 16S rRNA gene. The identification of morphological observations, gram staining, and biochemical tests showed that probiotic bacteria isolates, including Gram-positive, rod-shaped, rounded colony form, flat elevation, entire nonmotile edge, and catalase-negative, could ferment all carbohydrate content in the TSIA medium. The antibacterial potential was also found in probiotic bacteria, as evidenced by the inhibition zone formed in the test. The results of the bacterial gene sequences of PaTa5 probiotic bacterial isolates had a similarity of 98.37% with Lactobacillus plantarum. These findings indicated the presence of some bacteria species that have antibacterial activity in the intestines of domestic chickens (Gallus domesticus).\u0000Keywords: Lactobacillus plantarum, Native chicken, Probiotic, 16S rRNA","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"33 1-2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133820564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Black pepper, Turmeric, and Fennel on Essential and Non-essential Chemical Contents of Egg","authors":"L. Samantaray, Y. Nayak","doi":"10.36380/jwpr.2023.6","DOIUrl":"https://doi.org/10.36380/jwpr.2023.6","url":null,"abstract":"The use of essential oils (EOs) in animal feeding has gained attention as a potential antibiotic growth promoter replacement over the past two decades. The current study aimed to determine the impact of three feed additives, namely black pepper, turmeric, and fennel, on the productivity of laying hens, the chemical composition, and macro- and microelement content in layer eggs. A total of 280 chicks aged 75 days were randomly divided into 7 treatments (5 replicates of 8 chicks). One treatment group was provided as the unsupplemented control. The other six treatment groups, namely D0 (basal diet [BD] control), D1 (BD + 1% of black pepper), D2 (BD + 1% of turmeric), D3 (BD + 1% of fennel), D4 (BD + 0.5% of black pepper + 0.5% of turmeric), D5 (BD + 0.5% of black pepper + 0.5% of fennel), D6 (BD + 0.5% of turmeric + 0.5% of fennel) were supplemented with varying levels of phytobiotics. The result of the study indicated that the egg weight, Hen-day-production (HDP), egg quality, and haugh unit significantly improved with a combined supplementation of phytobiotics (D4, D5, and D6 diets) when compared with the control. However, there were no significant differences in the chemical composition of eggs. The X-ray fluorescence spectrometer analysis of eggs revealed the presence of 17 significant elements, including phosphorous, sulfur, chlorine, potassium, calcium, manganese, iron, copper, zinc, and bromine. The study findings showed that the combined supplementation of phytobiotics lowered K and Cl, whereas Zn, Ca, S, and Cu contents positively increased in hen eggs by including phytobiotic in the diet. In conclusion, the EOs of phytobiotics as dietary supplementation at 1% and 0.5% could improve the HDP, egg weight, and egg mass, including nutrient elements in the egg.\u0000Keywords: Egg, Essential oil, Hen, Mineral, Phytobiotic","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124528843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Poultry Management Strategies to Alleviate Heat Stress in Hot Climates: A Review","authors":"Shame Bhawa, J. Morêki, James Butti Machete","doi":"10.36380/jwpr.2023.1","DOIUrl":"https://doi.org/10.36380/jwpr.2023.1","url":null,"abstract":"Heat stress remains a major challenge affecting poultry production in sub-tropical and tropical environments; hence it continues to receive attention. The present study aimed to discuss heat stress and its effects on poultry production and suggests mitigation strategies to combat the effects of increased environmental temperature on poultry performance. Poultry raised in hot climates suffers from heat stress, which reduces meat and egg production, reproductive performance, feed intake, and feed conversion efficiency leading to poor growth rates. Reduced feed intake results in a reduction in meat quality, growth, egg yield, and quality. A decrease in feed utilization efficiency is the major cause of poor growth performance in hot environments. To counteract the negative impacts of high ambient temperatures on the performance of poultry, a wide range of management practices are widely used, including nutrient manipulations (particularly protein and energy), electrolyte and vitamin supplementation, feed form (especially particle size and moisture content), choice feeding, controlled feeding, time of feeding, wet feeding, water management, and use of new breeds that thrive well in hot environments. These management practices help lower heat load and facilitate evaporative cooling, all of which may positively impact poultry performance and health.\u0000Keywords: Choice feeding, Feed conversion efficiency, Heat stress, Poultry production","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126896569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Ghaniei, S. Ghafouri, Soheil Sadr, Negar Hassanbeigi
{"title":"Investigating the Preventive Effect of Herbal Medicine (Allium sativum, Artemisia annua, and Quercus infectoria) against Coccidiosis in Broiler Chickens","authors":"A. Ghaniei, S. Ghafouri, Soheil Sadr, Negar Hassanbeigi","doi":"10.36380/jwpr.2023.10","DOIUrl":"https://doi.org/10.36380/jwpr.2023.10","url":null,"abstract":"Coccidiosis is a critical disease in the poultry industry worldwide. Producers apply different strategies to control and prevent this disease. Herbal drugs are suitable remedies for reducing losses associated with coccidiosis in poultry. The present study aimed to evaluate the effectiveness of an herbal mixture in preventing coccidiosis. A total of 160 broiler chickens were divided into four treatment groups, with five replicates for each. Experimental infection of all groups, except group D, was carried out with mixed Eimeria species (E. tenella, E. necatrix, E. brunetti, and E. maxima) on day 14. Broiler chickens in group A were given an herbal mixture (75% Quercus infectoria, 16% Artemisia annua, and 9% Allium sativum) as feed additives during the rearing period, and group B was treated with Monensin. No treatment was applied to group C after the chickens were experimentally infected with mixed Eimeria spp. Group D was used as the negative control since chickens in this group were not infected or sick during the experiment. Body weight gain, feed conversion ratio (FCR), mortality rate, intestinal lesion scoring, and oocyst count per gram (OPG) were evaluated in this study. The results of the present study revealed that the highest mean body weight was gained in group D, followed by chickens in group A. The best FCR results were attributed to chickens in group D, followed by group B. In this study, both drugs decreased mortality rate, intestinal lesion scores, and OPG in the treated chickens. In conclusion, this herbal mixture can reduce coccidial lesions.\u0000Keywords: Broiler, Coccidiosis, Herbal mixture, Prevention\u0000","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129648480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Rozi Khan Sadiq, Mohammad Azim Abrahimkhil, N. Rahimi, Sayed Ziaulhaq Banuree, Sayed Attaul Haq Banuree
{"title":"Effects of Dietary Supplementation of Vitamin E on Growth Performance and Immune System of Broiler Chickens","authors":"Rozi Khan Sadiq, Mohammad Azim Abrahimkhil, N. Rahimi, Sayed Ziaulhaq Banuree, Sayed Attaul Haq Banuree","doi":"10.36380/jwpr.2023.13","DOIUrl":"https://doi.org/10.36380/jwpr.2023.13","url":null,"abstract":"As a potent antioxidant, Vitamin E may lessen the potentially harmful consequences of such oxidative stress to protect broilers against immune-pathological damage. Broiler chicken growth and viability are enhanced by Vitamin E supplementation. The present study aimed to investigate the effects of Vitamin E dietary supplementation on broiler chickens’ growth performance and health status. A total of 48 one-day-old Ross chicks were randomly divided into two groups of control and treatment (supplementation of Vitamin E at a dose of 300 mg/kg diet) with three replicates per group. The study included an equal number of Ross breed chicks and Vitamin E dosage in two trials on two different dates (January and March, 2022). In both trials, the obtained results indicated no significant changes in weight gain in the control and treatment groups. In both trials, there were no significant differences in the spleen weight of the control and treated groups; however, from day 1 to 28 of the second trial, the bursa of Fabricius was heavier in the treated group than in the control group. Additionally, Vitamin E had no significant effects on the mitogenic responses to phytohemagglutinin (PHA) and Concanavalin A (Con A). Dosages of 20 and 10 µl for both PHA and Con A did not significantly affect the rate of pure lymphocyte proliferation in chicks fed 300 mg Vitamin E /kg feed. Cell-mediated immunity did not differ significantly between the two trials. The percentages of CD4, CD8, Bu1, and MHCII molecules in the spleen and cecal tonsil of the chicks that received Vitamin E 300 mg/kg feed did not change significantly. The antibody titers against infectious bronchitis and infectious bursal disease vaccines showed no significant differences. On day 42, there was a trend toward an increase in antibody titer in the case of the Newcastle disease vaccine. In conclusion, 300 mg/kg of Vitamin E added to the diet did not improve growth performance and immunity in broiler chicks.\u0000Keywords: Broiler chicken, Growth performance, Immune system, Vitamin E","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114799981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Lactobacillus-Fermented Feed on Production Performance and Carcass Quality of Broiler Chickens","authors":"R. Palupi, Fitri Novaliya Lubis, A. Pratama","doi":"10.36380/jwpr.2023.14","DOIUrl":"https://doi.org/10.36380/jwpr.2023.14","url":null,"abstract":"The quality of broiler chicken carcasses is greatly influenced by feed management and the number of nutrients digested in the digestive tract that will be utilized for optimal meat production. The study aimed to determine the effect of feeding fermented feed at different times on the production performance and quality of broiler chicken carcasses. The number of day-old chicks used in this study was 180 broiler chickens strain Cobb. This study was carried out experimentally using a complete randomized design consisting of four treatments and five replications. Each treatment carried out in this experiment consisted of a different length of time, namely feeding for 2, 3, 4, and 5 weeks. Experimental parameters included feed consumption, weight gain, and ration conversion. In addition, the carcass quality was investigated as live weight, carcass percentage, and percentage of broiler chicken abdominal fat. The results showed that the longer the time of fermentation feed significantly increased feed consumption by 40.07% and increased 13.77% weight gain, as well as decreased ration conversion by 25.33%. Furthermore, the same results were also obtained regarding live weight by 17.80% and increased percentage of the carcass by 8.84%, while the percentage of abdominal fat decreased by 12.90%. It can be concluded that the provision of fermented feed for 5 weeks can improve the production performance and carcass quality of broiler chickens.\u0000Keywords: Broiler chicken, Carcass quality, Fermented feed, Performance","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125069552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Essam Mohamed Hassan Karar, A. M. Atta, H. Gharib, M. A. El-Menawey
{"title":"Impact of Prebiotic Supplementation on Productive Performance, Carcass Traits, and Physiological Parameters of Broiler Chickens under High Stocking Density Condition","authors":"Essam Mohamed Hassan Karar, A. M. Atta, H. Gharib, M. A. El-Menawey","doi":"10.36380/jwpr.2023.5","DOIUrl":"https://doi.org/10.36380/jwpr.2023.5","url":null,"abstract":"The present study was performed to investigate the effect of increasing stocking density, prebiotic supplementation, and the interactions on broiler chicken performance and some physiological parameters. A total of 912 one-day-old chickens were used in this study, and they were randomly divided into six groups with 4 replicates each. The experiment included three levels of stocking densities (10, 13, and 15 broiler chicken/m2) in 6 groups. Groups 1, 3, and 5 were maintained without prebiotic supplementation, while groups 2, 4, and 6 received a diet supplemented with prebiotics in water (1cm/liter). Reducing stocking densities and adding prebiotics improved body weight, feed consumption, feed conversion ratio, hemoglobin, packed cell volume, oxidative stress parameters (total antioxidant capacity), and European production efficiency factor, while decreasing malondialdehyde levels. On the other hand, stocking density and prebiotic supplementation did not affect dressing percentage, the relative weight of giblet parts, hind part, front part, and lymphoid organs (thymus and bursa of Fabricius). In conclusion, adding prebiotics at 1 cm/liter (Mannan-oligo saccharide and B-Glucan) can partially mitigate the negative effects of high stocking density on production performance, physiological and oxidative stress parameters, and European production efficiency factor.\u0000Keywords: Antioxidant biomarkers, Broiler chicken, β-glucan, Mannan oligosaccharide, Oxidant, Prebiotic, Stocking density","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123967455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yelsi Listiana Dewi, A. Yuniza, K. Sayuti, M. Mahata
{"title":"Processing of Sargassum binderi Seaweed for Supplementation in Poultry Diet","authors":"Yelsi Listiana Dewi, A. Yuniza, K. Sayuti, M. Mahata","doi":"10.36380/jwpr.2023.11","DOIUrl":"https://doi.org/10.36380/jwpr.2023.11","url":null,"abstract":"Sargassum binderi has been potentially used as laying hen feed since it contains bioactive compounds useful for poultry health. In addition, the high alginate content of S. binderi has made it inappropriate for the poultry diet. Therefore, the alginate content should be reduced before its use in poultry feed. This study aimed to reduce the alginate of S. binderi for use as laying hen feed. The experiment was performed in two phases in a completely randomized design. The first phase included heated S. binderi in the autoclave and the second phase entailed the immersion of S. binderi in whiting filtrate. The treatments in the physical method contained a control group, and four treatment groups heating for 15, 30, 45, and 60 minutes. The treatments in the chemical method had a control group and four treatment groups with immersion periods of 1, 2, 3, and 4 hours. Each treatment was repeated five times, and the investigated parameters were crude protein, total dry matter, organic matter, ash, and alginate, respectively. The heating durations of S. binderi in an autoclave and different immersion periods of S. binderi in whiting filtrate did not significantly affect total dry matter, organic matter, ash, alginate, and crude protein. The results of this study showed that physical treatment (heat treatment) and chemical treatments (whiting filtrate immersion) did not have a significant effect on the alginate content, crude protein, ash, dry matter, and organic matter.\u0000Keywords: Alginate, Heating, Laying hen, Sargassum binderi, Whiting filtrate\u0000","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127532162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of a Vitamins D and C Supplement on Performance, Hatchability and Blood Profiles of Broiler Breeders","authors":"Nanik Setiyaningsih, A. Jayanegara, W. W. Wardani","doi":"10.36380/jwpr.2023.7","DOIUrl":"https://doi.org/10.36380/jwpr.2023.7","url":null,"abstract":"Vitamin D is a fat-soluble vitamin that plays a crucial role in controlling Calcium and Phosphor homeostasis, bone mineralization, and modulation of immune responses. Vitamin C is a cofactor of enzymatic reactions with anti-inflammatory and antioxidant properties to prevent and repair damage to cells in the body from exposure to free radicals and the immune system. The current study aimed to investigate the effects of dietary supplementation of 25(OH)D3 with vitamin C at different doses on broiler breeders’ blood profile, egg quality, and hatchability. The adaptation process before collecting the data was 2 weeks. A total of 6200 females and 620 male broiler breeders in the laying period aged 32-46 weeks were divided into 4 treatment groups with 5 replicates (each peach contained 310 female and 31 male breeders). The treatments included control as T0 (0 g/ton Nutricell HyC®), T1 (100 g/ton Nutricell HyC®), T2 (200 g/ton Nutricell HyC®), T3 (400 g/ton Nutricell HyC®) supplemented in feed. The observed variables were performance in breeding farms and hatcheries. The treatments with experimental doses indicated significant differences in the performance of broiler breeders, including feed intake, body weight, egg weight, egg mass, hen day production, hen house production, feed conversion ratio, and parameters of blood profile. The results showed a significant difference between the treatments and the control group in terms of hatch performance, clear eggs, exploding eggs, hatchability eggs, fertile eggs, salable chicks, and hatching of fertile eggs. However, no significant effects on fertility, culling of chicks, and embryonic mortality in the treatment groups were indicated. In conclusion, Nutricell HyC® with a dose of 400 g/ton in feed has indicated the best result in breeding farm and hatchery performance of broiler breeders in the laying period.\u0000Keywords: Blood profile, Broiler breeder, Calcidiol (25(OH)D3), Nutricell HyC®, Performance, Vitamin C\u0000","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129147529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Sex and Rearing Season on Body Weight Gain and Growth Curve Parameters of Local Chickens in Niger","authors":"","doi":"10.36380/jwpr.2023.15","DOIUrl":"https://doi.org/10.36380/jwpr.2023.15","url":null,"abstract":"Local chicken breeding is widespread in Niger, a country with harsh environmental conditions. This study aimed to investigate the effects of sex, temperature, and hygrometry variations on the body weight gain and growth curve of local Nigerien chickens. Two groups of local chickens were followed from hatching to 20 weeks of age. The first and second groups consisted of 96 and 124 chickens, respectively. Three seasons were identified based on continuously recording ambient temperature and humidity over a year. The dry and warm seasons (February, March, April, and May), the wet and warm seasons (June, July, August, and September), and the dry and cold seasons (October, November, December, and January). The average hatch weight was about 24 g, and monthly body weight gains ranged from 100 to 360 g. Asymptotic weights were 2214.02 ± 69.94 g and 1776.93 ± 63.57 g for roosters and 1380.25 ± 25.96 g and 1433.08 ± 71.24 g for hens. The sexual maturity rates indicate that hens are more precocious than roosters. Sex and season had significant impacts on the growth performance of the chickens. In conclusion, the results of the present study indicated that the optimal time to raise local chickens in rural Niger is from June to January, and males are better candidates for meat production.\u0000Keywords: Growth curve, Hygrometry, Local chicken, Temperature, Weight gain","PeriodicalId":290171,"journal":{"name":"Journal of World's Poultry Research","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127674922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}