BERITA BIOLOGIPub Date : 2019-04-22DOI: 10.14203/beritabiologi.v18i1.3107
Syarifa Nurdawati, Z. Fahmi, F. Supriyadi
{"title":"PARAMETER POPULASI IKAN BETOK (Anabas testudineus (BLOCH, 1792)) DI EKOSISTEM PAPARAN BANJIR SUNGAI MUSI, Lubuk Lampam","authors":"Syarifa Nurdawati, Z. Fahmi, F. Supriyadi","doi":"10.14203/beritabiologi.v18i1.3107","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i1.3107","url":null,"abstract":"Anabas testudineus Bloch, 1792 is economically important fish species found in Lubuk Lampam floodplains of Musi River. Fishing activities that take place throughout the year can affect reproduction patterns and recruitment (new additions) of fish. This study aims to evaluate several parameters of betok fish populations that can be used as input for fish resource management policies in floodplains of Musi river. A total of 3271 samples of betok fish caught by fishermen from March to November 2011 were collected to obtain data on fish length and weight. Growth parameters (L∞, K, Z dan M) were calculated using FiSAT II based on the length frequency distribution of fish. The values of L∞ and K were obtained at 204.75 mm and 0.24 / year respectively while the maximum age of fish was estimated to be 4.36 years. The total mortality (Z) and natural mortality (M) of betok fish is 0.99 / year and 0.42 / year, Fishing mortality (0.56/year). The exploitation rate (0.57) was slightly lower than the predicted value (Emax= 0.69) indicating that Anabas testudineus was near fully exploited in the Lubuk Lampam floodplain ecosystem. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"221 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116417010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2019-04-22DOI: 10.14203/BERITABIOLOGI.V18I1.3714
Jarulis Jarulis, Dedy Duryadi Solihin, Ani Mardiastuti, Lilik Budi Prasetyo
{"title":"VARIASI INTERSPESIFIK JULANG (AVES: BUCEROTIDAE) INDONESIA BERDASARKAN GEN CYTOCHROME-B DNA MITOKONDRIA","authors":"Jarulis Jarulis, Dedy Duryadi Solihin, Ani Mardiastuti, Lilik Budi Prasetyo","doi":"10.14203/BERITABIOLOGI.V18I1.3714","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V18I1.3714","url":null,"abstract":"Genetic information of Indonesian hornbills (Julang) is still limited. We sequenced the cytochrome b gene of three hornbill species (Rhyticeros undulatus, R. plicatus, and Aceros cassidix) to explore their genetic variation, distance, and phylogenetic. Nine blood samples for Cyt b from three hornbill species were isolated with Dneasy® Blood and Tissue Kit base on Spin-Column Protocol, Qiagen. SNP, genetic distance, and phylogeny were calculated by MEGA 6.0 software. This study was found variations in the Cyt b gene sequence (849 bp) between three species Indonesian Julang. Rhyticeros undulatus had the most SNP compared to R. plicatus and Aceros cassidix. Substitution of synonyms is more common than substitution of synonyms and transition substitution frequency tends to be much higher than transversion substitution. The three species of Indonesian Julang were markedly separate with an average genetic distance of 5.25%. and separated with the outgroup species at a genetic distance of 9.43%. Cytochrome b sequence genes from these three hornbill species are novel for identifying and classifying hornbills, and could be applied to prevent illegal poaching and conservation management in Indonesia. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125897033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2019-04-22DOI: 10.14203/beritabiologi.v18i1.3233
Siti Yuriyah, D. W. Utami, S. Nurani, A. Nasution, P. Lestari, A. Dadang, S. Suwarno
{"title":"SELEKSI BERBANTUKAN MARKA MOLEKULER UNTUK PEMBENTUKAN CALON VARIETAS PADI TURUNAN ESENSIAL SITU PATENGGANG","authors":"Siti Yuriyah, D. W. Utami, S. Nurani, A. Nasution, P. Lestari, A. Dadang, S. Suwarno","doi":"10.14203/beritabiologi.v18i1.3233","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i1.3233","url":null,"abstract":"The utilization of molecular marker technology for Blast (Pyricularia grisea) resistance in rice breeding could accelerate and improve the precision of selection. This study aimed to identify and to select the BC3F2 progenies from Situ Patenggang and the opted Blast Monogenic Lines based on their resistance to blast disease in green house and field using molecular characterization. A total of 200-300 BC3F2 population strains resulted in 4 crosses between Situ Patenggang varieties and Monogenic Varieties IRBLta2-Re, IRBLkp-k60, IRBLi-F5 and IRBLa-A were used as total genetic material in the study. Blast resistance assay was initially carried out in a greenhouse and further selection was conducted in the endemic blast area, Sukabumi. The selected Molecular marker was STS (Sequence Taq Sites) marker for foreground selection and 384-SNPs (Single Nucleotide Polymorphism) chip for background selection. The number of lines from each cross was selected by greenhouse assay i.e: 25 lines derived from Situ Patenggang/IRBLta and Situ Patenggang/IRBLkp, 21 plants for crossing Situ Patenggang/IRBLi, and 22 plants for Situ Patenggang/IRBLa-A. The results of field experiment revealed that blast resistance response of the selected lines was varied from 0 to 5. Lines of foreground were successfully selected using STS markers specified for Pii, Pita, Pikp and Pia genes. As for the background selection by SNPs markers, some lines carried the recurrent parent genetic background, Situ Patenggang. Overall, 20 resistance lines that harbored the target genes and Situ Patenggang background were obtained. Further observation was entailed to these twenty selected lines in order to attain promising lines candidate for blast resistance. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132094407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/beritabiologi.v17i3.2922
E. Maftu’ah, A. Susilawati
{"title":"BIOLEACHING UNTUK MENINGKATKAN PRODUKTIVITAS LAHAN SULFAT MASAM AKTUAL UNTUK TANAMAN PADI","authors":"E. Maftu’ah, A. Susilawati","doi":"10.14203/beritabiologi.v17i3.2922","DOIUrl":"https://doi.org/10.14203/beritabiologi.v17i3.2922","url":null,"abstract":"Technology of microbial utilization to accelerate the oxidation of pyrite followed by leaching (bioleaching) has the potential to resolve problems in land management of actual acid sulphate soil. The research aims to obtain a bioleaching technology package that can improve the productivity of the actual acid sulfate soil. The experiment was conducted on tidal swamp land that has soil type of actual acid sulfate at Wana Raya sub-district, district Batola, South Kalimantan, in July - November, 2014. The study was designed by using strip plot with three replications. The treatment consisted of two factors, namely application of an oxidizing microbial pyrite and leaching amount (intensity). The main plot consisted of (P0) without leacing (naturally), (P1) six times leaching , (P2) eight times leaching, and (P3) 12 times leaching. Subplot consisted of (M0) without oxidizing microbial pyrite, (M1) with oxidizing microbial plant used in this study is Inpara 3 of rice variety. Observations were made on soil pH, oxidizing bacteria pyrite, growth of rice plants (plant height and number of tillers) and rice yield. The results showed that the main problem of actual acid sulfate land studied is a high soil acidity (pH of 3.44). Influ-ence of leaching was greater than microbial application on crop yields. Application of the pyrite oxidizing bacterial and leaching eight times gave the best influence on the growth and yield of rice plants.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130822484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/BERITABIOLOGI.V17I3.3366
W. Wardani
{"title":"KAJIAN POTENSI PRODUKSI AKAR ADVENTIF PAKIS POHON Cyathea contaminans (CYATHEACEAE) DI JAWA BARAT DAN SUMATERA UTARA","authors":"W. Wardani","doi":"10.14203/BERITABIOLOGI.V17I3.3366","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V17I3.3366","url":null,"abstract":"Study on production potential of adventitious root of the scaly tree fern Cyathea contaminans was conducted in six locations in West Java and North Sumatra using 20 m x 50 m sampling plot. Data recorded in the study consisted of population density, individual heights and diameter, volume of adventitious roots and environmental factors. The highest population density was found in a population that consisted of saplings with the height of less than 2 m tall. While population with adult plants has the density of 26–36 individual/100 m2. The wighest average volume of adventitious root per individuals was found in a population that dominated by adult stands and lies on a gentle slope at 108,760.70 cm3/individuals, while the lowest was in a population that consisted of saplings. The result statistical analysis showed that production of adventitious root were significantly different in different habitat. Moreover, there was positive correlation between trunk diameter and individual height with the volume of adventitious root, and between the diameter with individual height.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124044542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/BERITABIOLOGI.V17I3.3434
Purwaningsih Purwaningsih, Tika D. Atikah
{"title":"DIVERSITAS FLORISTIK DAN STRUKTUR VEGETASI DI HUTAN GUNUNG PAYUNG, TAMAN NASIONAL UJUNG KULON","authors":"Purwaningsih Purwaningsih, Tika D. Atikah","doi":"10.14203/BERITABIOLOGI.V17I3.3434","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V17I3.3434","url":null,"abstract":"A floristic diversity and vegetation forest structure was carried out on the Mount Payung, Ujung Kulon National Park. The objective was to floristic composition and structure of the forest located at the peninsula of Ujung Kulon, Banten. The study was conducted using the quadrat method by establishing plots of 5000 m2 each at two locations Pasir Ipis and Gunung Keneng, thus the total area sampled was 1 ha. They were Plot Pasir Ipis and Plot Gunung Keneng. A total of 1898 trees comprising 105 species of 74 genera and 43 families were recorded. The number of species at Pasir Ipis higher (90 species) than plot Gn Keneng (61 species). Based on calculation the important value indexes (IVI), there were some dominan species with IVI >10 at Pasir Ipis Neesia altissima (IV=21,55), Barringtonia racemosa (17,89), Payena acuminata (13,35) and Neonauclea lanceolata (12,37). The dominated species at Plot Gn Kendeng were Pseuduvaria reticulata (IV= 34,30), Dillenia excelsa (27,62), Lagerstroemia speciosa (20,89), Popowia pisocarpa (17,57). The structure of forest could be inferred from the diameter and height of trees. The diameter measurements showed that 75,57 % of trees in plot Pasir Ipis and 80,51 % in plot Gunung Kendeng consist of small individuals with diameters between 10-20 cm. Trees with large diameters of >100 cm occurred in two plots among them Barringtonia racemosa, Polyalthia lateriflora, Popowia pisocarpa, Artocarpus elasticus. The presence of large numbers of small trees and lesser numbers of trees with large diameters in a forest stand indicated that the stand was regenerating after heavy disturbance. The presence of the majority of trees with height of < 20 m (99 %) further confirmed the forest’s dynamic status.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126574041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ISOLASI DAN IDENTIFIKASI KAPANG ENDOFIT ASAL AKAR TANAMAN KUNYIT (Curcuma longa) SEBAGAI ANTIMALARIA","authors":"Eris Septiana, Fauzy Rachman, Sylvia J.R. Lekatompessy, Harmastini Sukiman, Partomuan Simanjuntak","doi":"10.14203/BERITABIOLOGI.V17I3.3408","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V17I3.3408","url":null,"abstract":"Malaria is still the leading cause of death worldwide with nearly half the world's population at risk. Parasitic resistance to existing antimalarial drugs in the market makes the search for a source of new drugs from nature is very important. Therefore, the aims of this study are to determine in vitro antimalarial activity of endophytic fungi extract from turmeric root and to identify the selected isolate molecularly. Heme polymerization inhibition method was used as in vitro antimalarial assay. The selected isolate was thrn identified using ITS1, ITS2, and 5.8S sequences of rDNA. The result of this study obtained 16 isolates of endophytic fungi from root of turmeric plant with isolate code were of K.Cl.Sb.A1 - K.Cl.Sb.A16. All of the ethyl acetate extracts of isolated endophytic fungi have heme polymerization inhibition activity. K.Cl.Sb.A11 was the most active isolate on heme polymerization inhibition test with 94,31% at concentration of test material at 8 mg/mL and IC50 value at 1.84 mg/mL. Molecular analysis showed that K.Cl.Sb.A11 isolate was Penicillium sp. and potentially developed as an antimalarial drug.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131928193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/BERITABIOLOGI.V17I3.3305
H. S. Suwoyo, S. R. H. Mulyaningrum, R. Syah
{"title":"PERTUMBUHAN, SINTASAN DAN PRODUKSI IKAN NILA MERAH (Oreochromis niloticus) YANG DIBERI KOMBINASI PAKAN KOMERSIL DAN AMPAS TAHU HASIL FERMENTASI","authors":"H. S. Suwoyo, S. R. H. Mulyaningrum, R. Syah","doi":"10.14203/BERITABIOLOGI.V17I3.3305","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V17I3.3305","url":null,"abstract":"The study aimed to evaluate the combination between commercial feed and fermented tofu waste feeding for growth, survival rate and production of red Tilapia Oreochromis niloticus in pond. The study was conducted in Experimental Pond Installation of Research Institute for Coastal Aquaculture, Maros, South Sulawesi, using eight ponds with 2,500 m2 in size. Red Tilapia fingerlings (±8 cm in length) were reared for 112 days with 1 individu/m2 of stocking density. The feeding treatments were of 100% commercial feed (A), 75% commercial feed + 25% fermented tofu waste (B), 50% commercial feed + 50% fermented tofu waste (C), and 25% commercial feed + 75% fermented tofu waste (D). Feeding frequency was given twice a day using concentration 10% of biomass weight in the first month, and decreased by 3% of biomass weight in the fourth month. Experimental was designed by a completely randomized designed consisted of four treatments and two replications for each treatment. Observed variables were growth, survival rate, feed conversion ratio, production, and water quality. Present study indicated that different levels of combination of commercial feed and fermented tofu waste had significant effect on growth (P<0.05), but did not have significant effect on survival rate, production, and feed conversion ratio (P>0.05). Feeding by combination of 75% commercial feed + 25% fermented tofu waste could be utilized optimally by Tilapia. During experiment, pond water quality was in conducive condition to support the growth and survival rate of red Tilapia.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"416 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116680186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/BERITABIOLOGI.V17I3.3333
Rizka Rahmaida, M. Amelia
{"title":"PENGARUH KOLABORASI TERHADAP KUALITAS PUBLIKASI PENELITIAN KEANEKARAGAMAN HAYATI INDONESIA BERDASARKAN BASIS DATA SCOPUS (1990-2012)","authors":"Rizka Rahmaida, M. Amelia","doi":"10.14203/BERITABIOLOGI.V17I3.3333","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V17I3.3333","url":null,"abstract":"Collaboration becomes trend in scientific publication for last decade. Many previous studies showed that collaboration had impact on quality of publication in terms of citation impact. This study aimed to investigate the influence of different pattern of collaboration on the citation impact of publications on biodiversity research from Indonesian researcher. A one thousand six hundred and ninty nine (1,699) articles were published by researchers affiliated with institution located in Indonesia from 1990-2012 based on Scopus database. Different pattern of collaboration were investigated in different level. Based on the result, only 4.4% of those publications were single author publications, 11.4% of those were intra-institution publications, and 17.4% of those were domestic collaboration. Using linear regression analysis, the result of the study showed that there was a significant positive correlation between the number of authors and the number of citations in international publication on biodiversity research from Indonesian researcher. In addition, publications with a higher number of institutions have received higher number of citations. Publication with a higher number of foreign collaborating countries also received more citations.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125858001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BERITA BIOLOGIPub Date : 2018-12-31DOI: 10.14203/beritabiologi.v17i3.2933
Koesrini Koesrini
{"title":"ADAPTASI DAN KERAGAAN HASIL PADI VARIETAS INPARA DI LAHAN RAWA","authors":"Koesrini Koesrini","doi":"10.14203/beritabiologi.v17i3.2933","DOIUrl":"https://doi.org/10.14203/beritabiologi.v17i3.2933","url":null,"abstract":"Inpara is a rice variety rice released by the Indonesian Agency for Agricultural Research and Development (IAARD) which is adaptive to swamp land. The research objective was to evaluate the adaptability and yield performanceof Inpara varieties in swamp lands. The field experiment was conducted in tidal swamp lands, in Barito Kuala District and in swampy lands, Hulu Sungai Selatan District of South Kalimantan, during dry season of 2014. The research was arranged in a randomized complete design with three replications. The treatment was 7 Inpara varieties, i.e. Inpara 1, 2, 3, 4, 5, 6, 7 and comparison varieties, i.e Margasari in tidal swamp lands and Ciherang in swampy lands. The result showed that there was a variation adaptation between Inpara varieties in tidal swamp land and swampy lands. Inpara 2 and Inpara 3 varieties showed a good adaptation in tidal swamp lands, had the yield of 4.04 t/ha which an increasing yield was 35.9% higher than Margasari variety. Inpara 1 and Inpara 6 varieties presented a good adaptation in swampy lands that produced 2.118 t/ha and 2.275 t/ha and 1.9% and 9.5%, respectively higher than Ciherang variety. Variety of Inpara is not adaptive in swamp lands.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124890581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}