BERITA BIOLOGI最新文献_第6页

PERKEMBANGAN TEKNOLOGI SEL MAMALIA CHINESE HAMSTER OVARY (CHO) UNTUK PRODUKSI OBAT BERBASIS PROTEIN 哺乳类哺乳类动物的细胞技术改进，用于蛋白质类药物的生产

BERITA BIOLOGI Pub Date : 2019-08-27 DOI: 10.14203/beritabiologi.v18i2.3705

Adi Santoso

{"title":"PERKEMBANGAN TEKNOLOGI SEL MAMALIA CHINESE HAMSTER OVARY (CHO) UNTUK PRODUKSI OBAT BERBASIS PROTEIN","authors":"Adi Santoso","doi":"10.14203/beritabiologi.v18i2.3705","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i2.3705","url":null,"abstract":"Chinese hamsters ovary (CHO) and its derivative such as CHO-DXB11 cells, CHO-K1, CHO-DG44 and CHO-S are mammalian cells that are often used for production of therapeutic protein drugs. The CHO cells often used for protein production have several advantages including 1) host cells that are safe to use in drug production, 2) the level of production of proteins produced can be increased by amplifying genes using methotrexate (MTX), 3) having the capacity to make post-translation modificationsand 4) CHO cells can be adapted to grow in suspension. The high need for protein-based drugs triggers the development of basic knowledge and innovation in production of recombinant proteins. The impressive technological advances in CHO cell technology have made these cells can be used to produce proteins around 10 g/liter in order to meet the market demand. The first protein successfully produced using CHO mammalian cells was the therapeutic Tissue Plasminogen Activator (r-tPA, Activase) protein used for stroke patients. The presence of this drug is quickly followed by several other types of drugs. In this review, history of development of CHO cells, the contribution of CHO cells to basic research, progress of effective line cell screening and development technology are discussed.","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"275 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120881843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

THE MACROFUNGI DIVERSITY AND THEIR POTENTIAL UTILIZATION IN TANGALE NATURE RESERVE GORONTALO PROVINCE 哥伦塔洛省tangale自然保护区大型真菌多样性及其潜在利用

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.3379

D. Arini, M. Christita, J. Kinho

{"title":"THE MACROFUNGI DIVERSITY AND THEIR POTENTIAL UTILIZATION IN TANGALE NATURE RESERVE GORONTALO PROVINCE","authors":"D. Arini, M. Christita, J. Kinho","doi":"10.14203/BERITABIOLOGI.V18I1.3379","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V18I1.3379","url":null,"abstract":"Tangale Nature reserve is a conservation area located in Gorontalo that have the biodiversity typical of Wallacea bioregion including macroscopic fungi. The purpose of this study was to identify the fungal species diversity found in the Tangale Nature reserve as well as its potential use, considering that the information on species diversity of fungi in the Wallacea region is very limited. This research was conducted using the cruise method by identifying macroscopic fungi along the hiking trail of Tangale Nature Reserve. The macroscopic fungi were observed for its morphological characteristic include the colour, diameter, surface of the veil, the shape of the stem, the length and diameter of the stem, the lamella, including ring and pore, type of lamella and the type of volva. The research recorded the substrates and the location where the fungus was found. Data were analyzed descriptively qualitative. The results of the research identified 28 species of macroscopic fungi that included to the division Ascomycota and Basidiomycota. It belongs to 16 families and 8 orders are Pezizales, Agaricales, Auriculariales, Boletales, Cantharellales, Gomphales, Polyporales, dan Russulales. Based on place to grow as much as 57,14% were found growing on decaying wood and 42,86% found growing in the soil/litter. Based on the potential of use, it has identified four species of mushrooms potentially as edible mushroom are Pleurotus ostreatus, Auricularia auricula, Ramaria formosa, and Polyporus arcularius. 11 species of mushrooms potentially as medicinal mushroom are Calvatia craniiformis, Scleroderma citrinum, Lenzites betulina, Microporus flabelliformis, Coriolus versicolor, Microporus xanthopus, and Albatrellus confluens. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125752999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

KONDISI PLANKTON PADA TAMBAK UDANG WINDU (Penaeus monodon FABRICIUS) DENGAN SUBSTRAT BERBEDA WINDU虾场的浮游生物具有不同的子囊

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.3496

E. A. Hendrajat, A. Sahrijanna

{"title":"KONDISI PLANKTON PADA TAMBAK UDANG WINDU (Penaeus monodon FABRICIUS) DENGAN SUBSTRAT BERBEDA","authors":"E. A. Hendrajat, A. Sahrijanna","doi":"10.14203/BERITABIOLOGI.V18I1.3496","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V18I1.3496","url":null,"abstract":"Plankton is one of water microorganisms that plays an important role as natural food for fish and shrimp and as an stability indicator of water or aquatic environment.The purpose of this study was to determine the composition and abundance of plankton in tiger shrimp ponds using concrete ponds without substrate, concrete ponds using sand substrate and ponds. Sampling of plankton and water quality was carried out from March to July 2016 at PunagaTakalar Experimental Pond at 6 ponds consisted of concrete ponds without 2 subplot of sand (pond A) and concrete pond with 2 subplate sand subplate (pond B) respectively measuring 1,000 m2 and a 2-square-meter plot of 2,500–3,000 m2 (pond C). The results showed that plankton abundance in pond A ranged from 37–349 individuals/L, pond B ranged from 35–1,399 individuals/L and pond C ranged from 54–999 individuals/L. The most common phytoplankton genera in this study was Oscillatoria while for zooplankton is of Acartia. The diversity of plankton in ponds A, B and C is included in the community of unstable to moderate biota. In general, the diversity of plankton in pond B is included in the community of moderate biota. The plankton uniformity index in pond A, pond B and pond C is generally close to 1 this indicates that the existence of plankton species in the three ponds is relatively even. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130766845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KAJIAN PERSEBARAN dan POTENSI JATI PASIR (Guettarda speciosa L.) di INDONESIA

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.3595

Inggit Puji Astuti, Ratna Susandarini

引用次数: 0

PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE) 青瓷病原Lecanicillium sp. PTN01对螺旋体armigera (HUBNER)(鳞翅目:野草科)

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.3378

S. Ginting, T. Santoso, Yayi Munara, R. Anwar, L. Sudirman

{"title":"PATOGENISITAS CENDAWAN Lecanicillium sp. PTN01 TERHADAP PENGGEREK TONGKOL JAGUNG Helicoverpa armigera (HUBNER) (LEPIDOPTERA: NOCTUIDAE)","authors":"S. Ginting, T. Santoso, Yayi Munara, R. Anwar, L. Sudirman","doi":"10.14203/BERITABIOLOGI.V18I1.3378","DOIUrl":"https://doi.org/10.14203/BERITABIOLOGI.V18I1.3378","url":null,"abstract":"Helicoverpa armigera is one of the most important agricultural pests because it has a high reproductive rate and resulted in economic losses. One of the H. armigera control techniques that is in accord with IPM principle is the utilizing of entomopathogenic fungus. The aim of this research was to test the pathogenicity of Lecanicillium (Lecanicillium sp. PTN01) against H. armigera. Pathogenicity test was conducted by conidia application of Lecanicillium sp. PTN01 on both the larvae and eggs at density of 105, 106, 107 conidia/ml. The control group was only treated with steril water. Polymerase chain reaction (PCR) was performed for molecular identification Lecanicillium sp. PTN01. The results showed that Lecanicillium sp. PTN01 able to inhibit egg hatching 13.75%, and cause mortality of first instar larval survival 98.75%. The result of virulence test to the highest larval mortality at 107 conidia/ml density was 41,25%, with the values of LT25, 50, 75 were 3.95, 7.12, 12.82 (days) and LC25, 50, 75 were 4.6 x 105, 1.7 x 106, 4.6 x 109 (conidia/ml). The DNA sequence analysis of ITS 1 and ITS 4 primers showed that Lecanicillium sp. PTN01 was similar to fungus species L. kalimantanense strain BTCC F23 with 94% homology. ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114065387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STRUKTUR TULANG DAN OTOT SIRIP KAUDAL KOMPLEKS Andamia heteroptera Bleeker (IKAN AMFIBI)

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.2208

G. Susanto

引用次数: 0

HUBUNGAN SEBARAN MAMALIA KECIL DENGAN KONDISI LINGKUNGAN DI HULU DAS CITANDUY, JAWA BARAT 一种小型哺乳动物与西爪哇省CITANDUY上游环境条件的关系

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.3376

Maharadatunkamsi Maharadatunkamsi

引用次数: 0

PENGARUH SUGARCANE STREAK MOSAIC VIRUS TERHADAP ANATOMI DAN KADAR KLOROFIL DAUN BEBERAPA AKSESI TEBU (Sacharrum officinarum)

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.2288

R. Hamida, C. Suhara

引用次数: 1

ESTIMASI HERITABILITAS DAN RESPONS SELEKSI IKAN NILA HITAM (Oreochromis niloticus) DI TAMBAK 黑罗非鱼(学名Oreochromis niloticus)在渔场的估计性和反应反应

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/beritabiologi.v18i1.3540

Adam Robisalmi

{"title":"ESTIMASI HERITABILITAS DAN RESPONS SELEKSI IKAN NILA HITAM (Oreochromis niloticus) DI TAMBAK","authors":"Adam Robisalmi","doi":"10.14203/beritabiologi.v18i1.3540","DOIUrl":"https://doi.org/10.14203/beritabiologi.v18i1.3540","url":null,"abstract":"Genetic improvement in the establishment of a rapid growth strain of Nile tilapia might be achieved through selective breeding program This research was aimed to estimate heritability and response selection of nile tilapia in brackishwater pond. Breeding activity was carried out in fresh water in hapa 2x1x1 m. Males and females were breeding in pairs with a ratio of 1: 1. The number of established families was 19 families of 50 broodstock pairs. Harvesting of larvae was done on day 14. Larvae were collected and then kept in net 2 x 2 x1 m with stocking density 125 fish/m2 for 60 days. The next stage was sexing by grouping the male and female populations in each family. Grow out was carried out in net cage (3 x 5 x1.5 m ) that were placed in brackiswater pond at salinity 25–30 g/L with density 10 fish/m2. Selection of broodstock in the male and female populations was done on weight of 250–300 g. Cut off selection was determined on weight from 316 to 382g (male) and 221–315 g (female). The results of this study showed that the estimation value for heritability of male and female were 0.42 ± 0.22 and 0.42 ± 0.23 and the estimation value for response selection were 10.66% (male) and 10.92% (female). ","PeriodicalId":280065,"journal":{"name":"BERITA BIOLOGI","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130566906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PENINGKATAN SINTASAN LARVA IKAN KERAPU SUNU (Plectropomus leopardus) MELALUI MANAJEMEN PEMELIHARAAN YANG SESUAI 通过适当的维护管理增加石斑鱼幼虫期

BERITA BIOLOGI Pub Date : 2019-04-22 DOI: 10.14203/BERITABIOLOGI.V18I1.2951

Daniar Kusumawati, Y. Asih, Ketut Maha Seti

引用次数: 3