Analytical Chemistry最新文献_第5页

Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.5c00463

Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang, Yapiao Li, Rong-Bin Song, Zhaohui Li

{"title":"Target-Responsive Regulation of Bacteria-Surface Magnetic Element for Self-Powered Analysis of Aflatoxin B1 in Microbial Fuel Cell","authors":"Yuxin Wang, Jiale Sun, Cui Wang, Lingbo Qu, Lin Zhang, Yapiao Li, Rong-Bin Song, Zhaohui Li","doi":"10.1021/acs.analchem.5c00463","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00463","url":null,"abstract":"The limitation of the sensing mode greatly restricts the detectable species and detection specificity of microbial fuel cell-based self-powered biosensors (MFC-SPBs). Herein, we develop a bacterial quantity change-based sensing mode for MFC-SPBs, in which the Fe3O4@Au content modified on exoelectrogenic bacteria is designed to correlate with analyte concentration for regulating the bacterial numbers absorbed onto the magnetic auxiliary anode. The polydopamine and Au nanoparticles comodified bacteria are attached with complementary DNA for hybridization with aptamer-modified Fe3O4@Au nanospheres. When aflatoxin B1 (AFB1) is used as the model analyte, its appearance can cause the liberation of Fe3O4@Au nanospheres from bacteria due to aptamer recognition. Furthermore, introduced exonuclease I can achieve a recycling amplification effect, intensifying the release of Fe3O4@Au nanospheres. With the decrease in bacteria-surface Fe3O4 content, bacteria that can be adsorbed onto the anode in a magnetic field will be reduced, leading to a decrease in the performance of MFC-SPBs. The results show that the developed MFC-SPBs can quantitatively determine AFB1 with a limit of detection of 5 nM (S/N = 3). Also, the MFC-SPBs show good detection specificity and can assess AFB1 in peanut samples. Considering the good specificity and species diversity of aptamers, we believe that this developed sensing mode will receive wide attention in the field of MFC-SPBs.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"37 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-13 DOI: 10.1021/acs.analchem.4c05449

Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes, Dimitrios Sakellariou

{"title":"Leaching Kinetics of Iron-Rich Alkali-Activated Materials under Sulfuric Acid Attack: An In Situ Method Using Low-Field NMR Relaxometry","authors":"Ziyou Yu, Rodrigo de Oliveira-Silva, Everton Lucas Oliveira, Nana Wen, Yiannis Pontikes, Dimitrios Sakellariou","doi":"10.1021/acs.analchem.4c05449","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05449","url":null,"abstract":"Proton nuclear magnetic resonance (NMR) relaxometry is applied to monitor the temporal changes of Fe3+ ion concentration in an aqueous solution by exploiting the paramagnetic behavior of Fe3+ ions. The nondestructive and noninvasive nature of NMR techniques allows us to observe in situ the leaching behavior of iron-rich alkali-activated materials (AAMs) in sulfuric acid solution. By calibrating the relation between proton relaxation rates and Fe3+ ion concentrations, we can quantitatively measure the real-time release of Fe3+ ions into the acid solution. Traditional methods for estimating the acid resistance of AAMs are ex situ, often resulting in additional error and being time consuming. The proposed in situ NMR method is a novel and efficient complementary technique to traditional methods for studying the kinetics of acid attack on iron-rich AAMs. With the high temporal resolution of NMR measurements, the kinetics of Fe3+ release can be described by a proposed model that considers the sample’s geometry. Two iron-rich AAM samples with different calcium molar ratios are examined. It is found that in the “low Ca content” range (<20 mol %) AAM with higher calcium content has a lower apparent reaction rate constant, indicating greater resistance to sulfuric acid attack.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"13 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143827531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction 利用纳米电喷雾离子化和质子转移电荷还原法测定填充和空 AAV5 粒子的质量

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c01095

Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao, Scott A. McLuckey

{"title":"Mass Determination of Filled and Empty AAV5 Particles Enabled by Nanoelectrospray Ionization and Proton Transfer Charge Reduction","authors":"Nicolas J. Pizzala, Boukar K.S. Faye, Hangtian Song, Li Tao, Scott A. McLuckey","doi":"10.1021/acs.analchem.5c01095","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c01095","url":null,"abstract":"The mass determination of bio-ions that exceed a megadalton poses many challenges. While it is possible to generate gaseous ions from large biocomplexes, including intact viruses, via nanoelectrospray ionization (nESI), generating mass information using conventional ensemble measurements (i.e., from conventional mass spectra) requires the resolution of charge states. As biocomplexes increase in size, overlap of adjacent charge states becomes increasingly problematic. Single ion measurements that enable the simultaneous determination of mass/charge and charge can overcome the charge state overlap problem. However, ensemble measurements are, in principle, much faster. We demonstrate here the mass determination of empty and filled adeno-associated virus particles, serotype 5 (AAV5), both separately and as a mixture using nESI, gas-phase proton transfer ion/ion reactions, and time-of-flight mass analysis. The ion/ion reactions are used to reduce charge states to the point at which they can be resolved, and UniDec, a publicly available deconvolution program, is used to facilitate mass determination. This work demonstrates that mass measurements of binary mixtures of empty and filled AAV5 particles as large as 3.7–4.5 MDa can be enabled via the use of single proton transfer ion/ion reactions to facilitate charge state resolution.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"119 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c00162

Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu, Xiliang Luo

{"title":"Endogenous Enzyme-Activated Spatial Confinement DNA Nanowire with a Tumor Cell-Specific Response for High-Precision Imaging of the Tumor/Normal Cells Boundary","authors":"Lei Wang, Tingting Zhao, Congkai Wang, Xiaohan Xu, Wang Yao, Xiaozhe Pang, Shenghao Xu, Xiliang Luo","doi":"10.1021/acs.analchem.5c00162","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00162","url":null,"abstract":"Developing tumor cell-specific imaging approaches is essential for the clear delineation of tumor margins. However, traditional imaging approaches suffered from low reaction kinetics as well as limited tumor specificity resulting from their “always active” sensing mode, making it difficult to accurately depict tumor boundary. To address these limitations, we developed an endogenous enzyme-activated spatial confinement DNA nanowire probe (E-SCNW) with an enhanced tumor/normal cell discrimination ratio for high precision imaging of the tumor/normal cells boundary. The spatial confinement effect can improve reaction kinetics, and the endogenous enzyme-activation design can confine fluorescence response to the tumor cells region. Additionally, no additional cell delivery carriers were required during the cross of the cell membrane into the intracellular space. It is worth noting that benefiting from the spatial confinement effect and endogenous enzyme-activation design, the detection limit was decreased by nearly 25.6-fold and the tumor/normal cells discrimination ratio was enhanced by nearly 4.46-fold through using E-SCNW, indicating promising prospects in high-precision imaging of the tumor/normal cells boundary.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"20 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continuous, Label-Free Phenotyping of Single Cells Based on Antibody Interaction Profiling in Microfluidic Channels 基于微流控通道中抗体相互作用图谱的连续、无标记单细胞表型分析

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c00385

Thijs Roebroek, Willem Van Roy, Sophie Roth, Laura Chacon Orellana, Zhenxiang Luo, Youssef El Jerrari, Chad Arnett, Kasper Claes, Seungkyu Ha, Karolien Jans, Riet Labie, Ziduo Lin, Martin Obst, Deise Origuella, Van Pham, Frederic Van Bellinghen, Anil Vishnu Girikumar Krishna, Elnaz Vaezzadeh, Wiebe Vanhove, Joost Van Duppen, Guiquan Wang, Siri Willems, Peter Peumans, Murali Jayapala, Tim Stakenborg

{"title":"Continuous, Label-Free Phenotyping of Single Cells Based on Antibody Interaction Profiling in Microfluidic Channels","authors":"Thijs Roebroek, Willem Van Roy, Sophie Roth, Laura Chacon Orellana, Zhenxiang Luo, Youssef El Jerrari, Chad Arnett, Kasper Claes, Seungkyu Ha, Karolien Jans, Riet Labie, Ziduo Lin, Martin Obst, Deise Origuella, Van Pham, Frederic Van Bellinghen, Anil Vishnu Girikumar Krishna, Elnaz Vaezzadeh, Wiebe Vanhove, Joost Van Duppen, Guiquan Wang, Siri Willems, Peter Peumans, Murali Jayapala, Tim Stakenborg","doi":"10.1021/acs.analchem.5c00385","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00385","url":null,"abstract":"Flow cytometry commonly utilizes fluorescence labeling and extensive sample preparation to detect specific cell surface markers, making analysis under native cell conditions impractical. In this work, a label-free flow cytometry technique is presented that spatiotemporally resolves cell-surface interactions in antibody-coated microfluidic channels. Using computational imaging, numerous cells are tracked across a large field of view (12 × 3 mm2) and the resulting motion profiles are used for phenotypic cell characterization. As proof-of-principle, experiments targeting T-cell receptor CD8 are performed directly on cell cultures. Individual T-cells are successfully tracked in 98% cases for flow velocities of 1–3 mm·s–1. In 14 μm high channels coated with only nonspecific antibodies, both CD8-positive SUP-T1 and CD8-negative Jurkat cells exhibit mostly constant velocities. In contrast, using channels functionalized with CD8-specific antibodies, numerous CD8-positive cells but not CD8-negative cells show temporary delays in motion linked to surface interaction. Cell classification based on the observed interactions results in a clear contrast ratio of 23.9 ± 11.6 (mean ± standard deviation) between SUP-T1 and Jurkat cells at 1 mm·s–1. The contrast decreases at higher flow velocities as fewer cells interact due to the increased hydrodynamic lift. Our results affirm our method’s ability to differentiate cells without prior labeling or sample preparation.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"75 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoconfinement Effect and Nanozyme Catalysis Enhance ILu/HOF-14 Electrochemiluminescence for Biosensing

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c00794

Qianqian Cai, Yuehui Wang, Guifen Jie, Hongkun Li

{"title":"Nanoconfinement Effect and Nanozyme Catalysis Enhance ILu/HOF-14 Electrochemiluminescence for Biosensing","authors":"Qianqian Cai, Yuehui Wang, Guifen Jie, Hongkun Li","doi":"10.1021/acs.analchem.5c00794","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00794","url":null,"abstract":"A low collision frequency and an insufficient number of free radicals are the main problems leading to the low electroluminescence (ECL) efficiency of luminol and its derivatives. In order to solve the above issues, this work used nanoconfinement combined with nanozyme catalysis to significantly enhance the ECL efficiency. We assembled isoluminol (ILu) into the hydrogen-bonded organic framework HOF-14 and prepared a novel ECL emitter ILu/HOF-14 for the first time. Surprisingly, compared with the ILu/H2O2 system, the ECL signal of ILu/HOF-14/H2O2 was increased by 33 times. This was because the porous structure of HOF-14 effectively limited the movement of free radicals and increased their collision frequency. Therefore, the reaction rate between free radicals was significantly improved to achieve an ECL signal amplification. To further increase the number of free radicals, we introduced hybrid nanozyme Zn SAC@CuO2 NPs with superior peroxidase (POD)-like activity. It could effectively catalyze the coreactant H2O2 to produce a large amount of ROS (OH• and O2•–), accelerating the reaction rate of ILu with ROS and further improving the ECL signal. Based on the above research, a novel dual-mode biosensing and imaging platform was constructed to detect microcystin-LR (MC-LR). We used the nonspecific trans-cleavage activity of the CRISPR-Cas12a system to enhance the dynamic continuity and signal amplification capability of this biosensing platform, further improving the detection sensitivity and broadening the avenues of molecular diagnostic strategies.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"108 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glycosidase-Enabled Locus-Specific Detection of 8-Oxo-7,8-dihydroguanine in Genomes under Oxidative Stress

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-12 DOI: 10.1021/acs.analchem.5c00632

Tong-Tong Ji, Min Wang, Xia Guo, Fang-Yin Gang, Shan Zhang, Jun Xiong, Yao-Hua Gu, Neng-Bin Xie, Bi-Feng Yuan

{"title":"Glycosidase-Enabled Locus-Specific Detection of 8-Oxo-7,8-dihydroguanine in Genomes under Oxidative Stress","authors":"Tong-Tong Ji, Min Wang, Xia Guo, Fang-Yin Gang, Shan Zhang, Jun Xiong, Yao-Hua Gu, Neng-Bin Xie, Bi-Feng Yuan","doi":"10.1021/acs.analchem.5c00632","DOIUrl":"https://doi.org/10.1021/acs.analchem.5c00632","url":null,"abstract":"Oxidative DNA damage is closely linked to the onset of various age-related diseases. A significant oxidation product, 8-oxo-7,8-dihydroguanine (8OG), has been considered an important epigenetic-like marker in regulating gene expression. Accurately quantifying the locus-specific 8OG levels is crucial for understanding its functional roles in disease induction and gene regulation. In this study, we developed a glycosylase cleavage-mediated extension stalling (GCES) method for the locus-specific detection and quantification of 8OG in genomic DNA. This method utilizes the 8OG-DNA glycosylase Pab-AGOG, which induces single-strand breaks in DNA containing 8OG. The resulting cleavage is then assessed and quantified by using quantitative real-time PCR (qPCR). We successfully applied this strategy to evaluate locus-specific 8OG in synthesized DNA and genomic DNA from HEK293T, HeLa, and HepG2 cell lines under oxidative stress or triclosan treatment. The results demonstrate that the GCES method is accurate and suitable for the site-specific quantification of 8OG in both synthesized and genomic DNA from biological samples. We observed an increased level of 8OG in genomic DNA from biological samples treated with H2O2 or triclosan, indicating that both agents can elevate 8OG level in DNA. Overall, the GCES method provides a valuable, straightforward, and cost-effective tool for the quantitative detection of 8OG in DNA at base resolution, which facilitates the investigation of its functional roles as an epigenetic-like or DNA damage marker.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"5 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143822799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RapidMass: A Graphical Data Processing Application for Automated Species Authentication in High-Throughput Mass Spectrometry

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-11 DOI: 10.1021/acs.analchem.4c05062

Chunxiang Liu, Qian Meng, Yun Li, Hanze Wang, Huanya Yang, Huiting Ou, Saiyi Ye, Changliang Yao, Qirui Bi, Wenlong Wei, Jianqing Zhang, Dean Guo

{"title":"RapidMass: A Graphical Data Processing Application for Automated Species Authentication in High-Throughput Mass Spectrometry","authors":"Chunxiang Liu, Qian Meng, Yun Li, Hanze Wang, Huanya Yang, Huiting Ou, Saiyi Ye, Changliang Yao, Qirui Bi, Wenlong Wei, Jianqing Zhang, Dean Guo","doi":"10.1021/acs.analchem.4c05062","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c05062","url":null,"abstract":"High-throughput mass spectrometry (HT-MS) facilitates rapid, large-scale data acquisition, providing a fast and efficient solution for various analytical challenges. However, the increasing volume of data generated by MS requires automation and easy-to-use processing tools. Currently, there is no freely available software that is compatible with most instruments for species authentication or classification. Here, we introduce RapidMass, a cutting-edge software platform designed to automate the handling, evaluation, presentation, and management of HT-MS data for species identification. Key features include a streamlined workflow for processing spectra from various instruments (e.g., DI-MS, ASAP-MS, DART-MS), three specialized algorithms for scoring unknown samples, peak annotation review, visualization of MS1 and MS2 spectra, and an expandable personal database for customized data management. Performance validation conducted on nine data sets covering diverse sample compositions, instrument types, suppliers, resolutions, and MS acquisition modes, demonstrated RapidMass’s excellent performance, with processing times of 12 to 20 s per sample and authentication accuracies ranging from 97% to 100% for easily confused plants. With its user-friendly interface, RapidMass empowers users to create and manage personalized databases, presenting significant prospects for broad applications across various fields.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"26 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Novel Proteus-Specific, Piezoelectric Sensor Based on Newly Screened Targets

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-11 DOI: 10.1021/acs.analchem.4c07004

Hui Yu, Jinxia Ma, Yusheng Liao, Jiali Ren, Fengjiao He

引用次数: 0

Early-Stage Lung Cancer Diagnosis by a Point-of-Care Electrochemical Aptamer-Based Sensor of NAP2 in Human Serum

IF 7.4 1区化学

Analytical Chemistry Pub Date : 2025-04-11 DOI: 10.1021/acs.analchem.4c06815

Baixun He, Jie Fu, Chong Chen, Wenxin Zhang, Shuang Li, Cherie S. Tan, Dong Ming

{"title":"Early-Stage Lung Cancer Diagnosis by a Point-of-Care Electrochemical Aptamer-Based Sensor of NAP2 in Human Serum","authors":"Baixun He, Jie Fu, Chong Chen, Wenxin Zhang, Shuang Li, Cherie S. Tan, Dong Ming","doi":"10.1021/acs.analchem.4c06815","DOIUrl":"https://doi.org/10.1021/acs.analchem.4c06815","url":null,"abstract":"The target neutrophil-activating peptide-2, NAP2, is a potential biomarker for early lung cancer diagnosis, whereas there are currently no precise techniques for differentiating NAP2 from its precursors. To overcome this difficulty, we created an electrochemical aptamer-based sensor (E-AB) consisting of the 33-mer aptamer domain, a 2-bp three-junction region, and two conductive signal reporter stems. Whereas E-AB-AT and E-AB-RAN sensors with two (AT)6 or N12 stems, respectively, were unable to distinguish between platelet basic protein (PBP) (94 aa) and NAP2 (70 aa). However, in contrast, the E-AB-GC sensor with two (GC)6 stems could selectively detect NAP2 but hardly recorded PBP. Here, we developed an E-AB-GC point-of-care test (POCT) technique to detect NAP2 away from its precursors in 10 μL of human serum and provide concentration data in 5 min. Interestingly, serum NAP2 levels in human samples, as determined by the E-AB-GC sensor, were roughly 30–50% lower than those obtained by ELISA. Results also showed that E-AB-GC analysis of serum NAP2 in patients in stages I through IV revealed statistical significance and an excellent guiding function in the early diagnosis of lung cancer, particularly for patients in stage I cancer (p = 0.0054, area under the curve, 0.95). Importantly, this E-AB-GC POCT platform has shown potential as an on-site quick diagnostic tool, which can also be used to detect other lung cancer markers. Our research on the impacts of stem sequencing on sensing capabilities might assist in the future development of E-AB biomarker sensors.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"42 1","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143819848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0