Bulletin "Veterinary biotechnology"最新文献_第8页

Adjuvantas in construction of mixed vaccines against infectious enterites of calves 犊牛感染性肠炎混合疫苗的佐剂制备

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech35-11

P. Krasochko, Y. P. Yaromchyk, P. Krasochko, V. Krasochko, Y. Shashkova, N. V. Sinitsa, O. Tarasov

{"title":"Adjuvantas in construction of mixed vaccines against infectious enterites of calves","authors":"P. Krasochko, Y. P. Yaromchyk, P. Krasochko, V. Krasochko, Y. Shashkova, N. V. Sinitsa, O. Tarasov","doi":"10.31073/vet_biotech35-11","DOIUrl":"https://doi.org/10.31073/vet_biotech35-11","url":null,"abstract":"Introduction. an etiology of gastrointestinal tract diseases of calves the main role is played by viruses of infectious rhinotracheitis, diarrhea, rotavirus and coronavirus infection, escherichiosis and salmonellosis. The main method of their control of of adjuvants to increase their immunogenicity. A number of adjuvants – mineral (an aluminum hydroxide, potassium alum), oil (an incomplete Freund's adjuvant, эмульсиген, montanida, Marcala), natural polysaccharides is known (bacterial lipopolysaccharides, the activated cellulose) are known. In this regard selection of optimum adjuvant when designing vaccines is a current problem in biotechnology. vaccines against infectious gastroenteritis of calves, was established that all adjuvants had a positive impact on biosynthesis of specific antibodies. At the same time a vaccinated animals antiserum capacity increased by 4-7 log 2 . Conclusions and prospects of further researches. When choosing optimum adjuvant in the designed vaccines against gastroenteritis of an infectious etiology of calves, more pronounced stimulation of a humoral immune response were received when using oil IZA-15 and IZA-25 adjuvant.","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129230146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modern methods of foot-and-mouth disease diagnosis (review) 口蹄疫的现代诊断方法(综述)

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/VET_BIOTECH38-05

Z. Drozhzhe, Ia.N. Dzuba, G. Kyivska

{"title":"Modern methods of foot-and-mouth disease diagnosis (review)","authors":"Z. Drozhzhe, Ia.N. Dzuba, G. Kyivska","doi":"10.31073/VET_BIOTECH38-05","DOIUrl":"https://doi.org/10.31073/VET_BIOTECH38-05","url":null,"abstract":"development, improvement and application of modern methods for the diagnosis of FMD (PCR, ELISA). Results of research and discussion. Generally, a suspected case of FMD can be identified based on observations of clinical signs. However, diagnoses based on clinical symptoms are highly unreliable, because several other diseases share similar symptoms as FMD, which include swine vesicular disease (SVD), vesicular stomatitis and vesicular exanthema. Swine are vulnerable to vesicular stomatitis, SVD, and FMD, whereas cattle are vulnerable to vesicular stomatitis and FMD, all of which could not be distinguished based on clinical symptoms. Conventional techniques such as complement fixation test (CFT), virus isolation test, virus neutralization test (VNT), and ELISA are routinely used to detect FMDV in clinical samples. Advancement in molecular techniques accelerates rapid and accurate diagnoses of FMDV through detection of the viral RNA. In this article, the most recent advancements in reverse transcription-polymerase chain reaction (RT-PCR) and RT-loop-mediated isothermal amplification (RT-LAMP)-based methods are thoroughly reviewed. Lastly, the roles of lateral flow immunochromatographic (LFI) test strips in FMDV diagnosis are also discussed. Conclusions and prospects for further research. Various diagnostic methods ranging from conventional such as virus isolation and competitive- and blocking-antigen ELISA to molecular-based methods such as RT-PCR and RT-LAMP have been developed over the years. Although ELISA-based methods have good diagnostic sensitivity and specificity, molecular detection methods have the advantage of higher analytical sensitivity for the detection of minimal viral RNA.","PeriodicalId":274594,"journal":{"name":"Bulletin \"Veterinary biotechnology\"","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132263825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of ADG disinfectant cumulative and metabolic rate effect in laboratory animals ADG消毒液在实验动物体内累积和代谢率效应的评价

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech36-13

O. Nechyporenko, A. Berezovskiy, O. Shkromada, L. Ulko, S. Nychyk

引用次数: 1

The effect of acute and chronic course of daveniosis on biochemical indicators of hens’ blood serum 鹅肝病急、慢性病程对母鸡血清生化指标的影响

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech35-02

M. Bogach, V. Stoyanova

引用次数: 0

Indices of cellular immunity in case of рassalurosis of rabbits 兔腰尿症的细胞免疫指标

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech35-05

Y. Duda, M. Prus

引用次数: 2

Veterinary-sanitary evaluation of broiler chickens’ meat after adding chelated trace elements to their diet 肉仔鸡饲粮中添加螯合微量元素后肉品质的兽医卫生评价

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/VET_BIOTECH34-19

R. Timoshenko, T. Fotina, S. Nazarenko

引用次数: 1

Development of an express test for the detection of african swine fever virus antigen by the method of magnetic immunofluorescence analysis 磁免疫荧光法检测非洲猪瘟病毒抗原的快速试验的建立

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech42-09

O. Sereda, V. Spyrydonov, O. Tarasov, Y.P. Кrytsia, S. Nychyk

引用次数: 0

Dynamics of peroxidation processes in male rabbits under experimental LPS-induced oxidative stress 实验性lps诱导氧化应激下雄性家兔过氧化过程动力学

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech41-10

V. Koshevoy, S. Naumenko

引用次数: 1

Analysis of the epizootic situation of the swine erysipelas on the territory of Ukraine for 2006–2017 2006-2017年乌克兰境内猪丹毒流行情况分析

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/VET_BIOTECH34-13

N. Pinchuk, A. Golovko, T. Garkavenko

引用次数: 1

Efficiency of the postpartum cow pathology complex prevention schemes 产后奶牛病理复杂预防方案的有效性

Bulletin "Veterinary biotechnology" Pub Date : 1900-01-01 DOI: 10.31073/vet_biotech35-16

R. Sachuk, Ya. S. Stravsky, Yu.V. Goryuk, S. V. Zhigalyuk

引用次数: 0