Journal of Fundamental and Applied Pharmaceutical Science最新文献

Antioxidant Activity and Vitamin C Concentration Analysis of Gandaria (Bouae macrophylla Griff) Ethanol Extract Using Spectrophotometry UV Vis 紫外可见分光光度法分析甘草叶乙醇提取物的抗氧化活性及维生素C浓度

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2023-03-25 DOI: 10.18196/jfaps.v3i2.15992

Eva Kholifah, Dewi Nurazizah, Fajrin Noviyanto

{"title":"Antioxidant Activity and Vitamin C Concentration Analysis of Gandaria (Bouae macrophylla Griff) Ethanol Extract Using Spectrophotometry UV Vis","authors":"Eva Kholifah, Dewi Nurazizah, Fajrin Noviyanto","doi":"10.18196/jfaps.v3i2.15992","DOIUrl":"https://doi.org/10.18196/jfaps.v3i2.15992","url":null,"abstract":"Antioxidants are chemical compounds that donate one or more electrons to free radicals to inhibit free radical reactions. One of the potential antioxidant sources is gandaria fruit (Bouea Macrophylla Griff). This research aims to determine the antioxidant activity and vitamin C content of the gandaria fruit extract. Gandaria fruit was macerated with ethanol as a solvent. Furthermore, the rotated extract was carried out for the phytochemical screening. The results of the phytochemical screening of the gandaria fruit extract showed a positive of flavonoids, terpenoids, tannins, saponins, and phenolics. The gandaria fruit extract was tested for antioxidants and quantitative analysis of vitamin C levels to obtain IC50 values and vitamin C levels using spectrophotometry UV-Vis at a wavelength of 517nm with vitamin C as a positive control. The results of spectrophotometric measurements revealed that the gandaria fruit extract had an IC50 value of 5.72 g/ml, and vitamin C had an IC50 value of 2.260 g/ml, indicating that the gandaria fruit extract and vitamin C had very strong antioxidants. The value of vitamin C levels was 0.526 mg.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114468011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease 拟对症治疗镰状细胞病的Drepanoalpha®乙醇提取物、天竺葵和辣木植物药物的体外生物活性研究

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2023-03-25 DOI: 10.18196/jfaps.v3i2.16000

Benjamin Gbolo Zoawe, A. Nachtergael, D. Tshibangu, N. Misengabu, Victoire Nsabatien, P. Memvanga, D. Tshilanda, J. Ngbolua, P. Mpiana, P. Duez

{"title":"In Vitro Biological Activities of Drepanoalpha® Ethanolic Extract, A Justicia Secunda and Moringa Oleifera-Based Phytomedicine Proposed for The Symptomatic Treatment of Sickle Cell Disease","authors":"Benjamin Gbolo Zoawe, A. Nachtergael, D. Tshibangu, N. Misengabu, Victoire Nsabatien, P. Memvanga, D. Tshilanda, J. Ngbolua, P. Mpiana, P. Duez","doi":"10.18196/jfaps.v3i2.16000","DOIUrl":"https://doi.org/10.18196/jfaps.v3i2.16000","url":null,"abstract":"Sickle cell disease (SCD) is an autosomal recessive blood disorder characterized by red blood cells that assume an abnormal, rigid sickle shape under low-oxygen conditions. These sickle-shaped erythrocytes tend to lyse, aggregate, and obstruct small blood vessels, leading to major complications. The present study aims to investigate properties that may underlie the activity of Drepanoalphaâ, an antisickling herbal formulation developed in the Democratic Republic of Congo (DRC) for the prevention and symptomatic treatment of sickle cell disease crises. The Drepanoalpha® Ethanolic Extract (DEE) is a dry extract (drug-extract ratio, DER, 100/11) prepared from ethanol (96 %, v/v) percolation of a 1:1 mixture of 2 food plants, Justicia secunda Vahl and Moringa oleifera Lam. Sickling was classically measured by light microscopy on diluted washed erythrocytes obtained from homozygote patients; erythrocytes were treated with 2 % Na2S2O5 in the presence of DEE (suspension in 9 ‰ NaCl), 9 ‰ NaCl (negative control) or disodium cromoglycate (DSCG, positive control). For all tested conditions, the sickle hemoglobin polymerization, the Fe2+/Fe3+ ratio, and the median corpuscular fragility were measured by spectrophotometry. The DEE reversed sickling by 89.1 %, comparable to DSCG (87.7 %; 60.3 µg/mL), inhibiting sickle cell hemoglobin polymerization of 77.8 % and 74.4 %, respectively. The Fe2+/Fe3+ ratio was improved by 18.0 % for DEE and 15.9 % for DSCG. The median corpuscular fragility values were 0.602, 0.714, and 0.732 for NaCl 9 ‰, DSCG, and DEE, respectively. The measured in vitro parameters validate an effective antisickling effect of DEE and confirm the value of this improved traditional herbal formulation for the management of SCD.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129123856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phytochemical Analysis and Antioxidant Potential of Ethylacetate Extract of Tamarindus Indica (Tamarind) Leaves by Frap Assay Frap法分析罗望子叶乙酸乙酯提取物的植物化学及抗氧化活性

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2023-02-16 DOI: 10.18196/jfaps.v3i2.16708

{"title":"Phytochemical Analysis and Antioxidant Potential of Ethylacetate Extract of Tamarindus Indica (Tamarind) Leaves by Frap Assay","authors":"","doi":"10.18196/jfaps.v3i2.16708","DOIUrl":"https://doi.org/10.18196/jfaps.v3i2.16708","url":null,"abstract":"Oxidative stress is characterized by an imbalance in the generation of free radicals and their subsequent elimination by endogenous antioxidants. It is a characteristic of several diseases, especially during the progression stage, which can lead to fatal effects. This study aims to investigate the phytochemical components and antioxidant capability of Tamarindus indica and assess its capability as a candidate for managing diseases associated with oxidative stress. The gravimetric method detected and quantified phytochemicals, while the reducing power assay determined the antioxidant potential. Saponins, steroids, and flavonoids were detected in 6.83 ±0.44, 4.30 ±0.60, and 10.17% ±0.60, respectively, without alkaloids, glycosides, and terpenoids. The antioxidant test showed a concentration-dependent increase in absorbance of both the extract and standard (Ascorbic acid). However, Ascorbic acid had higher absorbance. At 100% concentration, the sample had an absorbance of 0.388 ±0.022, which was lower than the absorbance of Ascorbic acid (0.411 ±0.009) at 40% concentration. It can be concluded that Tamarind leaves could be utilized to manage diseases associated with oxidative stress, evidenced by their antioxidant potential credited to the phytochemical content of the leaves. However, there is a need for further studies to ascertain the exact compounds and their modes of action.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130362769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Total Flavonoid Content of Lemongrass Leaf (Cymbogoncitratus (DC.) Stapf) Extract and Antioxidant Activity with Frap 柠檬香茅叶总黄酮含量测定葡萄叶提取物及其抗氧化活性研究

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-31 DOI: 10.18196/jfaps.v3i1.15556

Ahda Maulida Ulufan Nurinnafi’a, Kusumaningtyas Siwi Artini, D. Permatasari

{"title":"Total Flavonoid Content of Lemongrass Leaf (Cymbogoncitratus (DC.) Stapf) Extract and Antioxidant Activity with Frap","authors":"Ahda Maulida Ulufan Nurinnafi’a, Kusumaningtyas Siwi Artini, D. Permatasari","doi":"10.18196/jfaps.v3i1.15556","DOIUrl":"https://doi.org/10.18196/jfaps.v3i1.15556","url":null,"abstract":"Lemongrass (Cymbogoncitratus (DC.) Stapf) leaves contain alkaloids, saponins, tannins, anthraquinones, steroids, phenols and flavonoids. Flavonoids act as antioxidants as they can reduce free radicals. This study aims to determine the total flavonoid content, antioxidant activity, and IC50 value of lemongrass leaf extract (Cymbogoncitratus (DC.) Stapf). Extracts were made by maceration using 96% ethanol as solvent. Testing of total flavonoid content with the AlCl3 method using UV-Vis spectrophotometry was carried out three times. The antioxidant activity test used the FRAP (ferric reducing antioxidant power) method on extracts containing 100, 200, 300, 400, and 500 ppm. The test results showed that the leaf extract of citronella (Cymbogoncitratus (DC.) Stapf) had a total flavonoid content of 22,60 mg QE/g extract. Furthermore, there was antioxidant activity in the leaf extract of lemongrass (Cymbogoncitratus (DC.) Stapf indicated by the formation of a blue color purplish when reacted with FRAP solution, and IC50 extract value was 71,59 ppm and included in the category of strong antioxidants.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"107 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115560052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Aqueous Leaf Extract of Chromolaena odorata Attenuates Methotrexate-Induced Hepatotoxicity in Wistar Rats 桔梗叶水提物减轻甲氨蝶呤所致Wistar大鼠肝毒性

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-31 DOI: 10.18196/jfaps.v3i1.15652

B. Ikponmwosa, Usunomena Usunobun

{"title":"Aqueous Leaf Extract of Chromolaena odorata Attenuates Methotrexate-Induced Hepatotoxicity in Wistar Rats","authors":"B. Ikponmwosa, Usunomena Usunobun","doi":"10.18196/jfaps.v3i1.15652","DOIUrl":"https://doi.org/10.18196/jfaps.v3i1.15652","url":null,"abstract":"Methotrexate (MTX) usage, despite its toxicity in body organs, has increased steadily over the years due to its broad applicability for treating different ailments, including various forms of cancer. Certain plant species have been shown to possess therapeutic properties by offering a protective effect against drug side effects. Thus, the current study was carried out to evaluate the potential of aqueous Chromolaena odorata leaf extract (AEOC) to attenuate the effect of MTX-induced hepatotoxicity in Wistar rats. The study divided thirty (30) male Wistar rats into five groups consisting of six each: Group I (control), Group II (AEOC at 250 mg/kg BW), Group III (MTX at 7 mg/kg BW), Group IV (AEOC at 250 mg/kg BW + MTX at 7 mg/kg BW), and Group V (Vitamin C (100 mg/kg BW) + MTX at 7 mg/kg BW). Chromolaena odorata and Vitamin C was administered for ten consecutive days, while MTX was administered on day 8 for three consecutive days. Rats were sacrificed 24hrs after the last administration. Serum collected was used for the determination of Aspartate Aminotransferase (AST), Alanine transaminase (ALT), Albumin (ALB), Total Bilirubin (TB), and Total protein (TP), while liver tissue was used for assessment of Superoxide Dismutase (SOD), Malondialdehyde (MDA) and Catalase (CAT) as well as histopathological analysis. The result showed a significant increase in the level of SOD, CAT and a significant reduction in MDA in Chromolaena odorata or Vitamin C treated groups compared with MTX. Furthermore, Chromolaena odorata or Vitamin C significantly reduced liver function enzymes and Total Bilirubin levels while increasing synthetic molecules compared to the MTX group. Chromolaena odorata attenuated the toxic effect of MTX, which was corroborated by histopathological analysis. In conclusion, Chromolaena odorata attenuated MTX-induced hepatotoxicity by enhancing antioxidant status; thus, scavenging free radicals and reducing oxidative stress.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"101 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133888508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Evaluation of Body Scrub Using Flour-Based from Fruits of Indramayu Variety of Cengkir Mango (Mangifera indica L) 以英德拉玛尤芒果(Mangifera indica L)果实为原料制备磨砂膏的配方及评价

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-31 DOI: 10.18196/jfaps.v3i1.15569

Dadan Hidayattullah, Ismanurrahman Hadi, Ade Irawan

{"title":"Formulation and Evaluation of Body Scrub Using Flour-Based from Fruits of Indramayu Variety of Cengkir Mango (Mangifera indica L)","authors":"Dadan Hidayattullah, Ismanurrahman Hadi, Ade Irawan","doi":"10.18196/jfaps.v3i1.15569","DOIUrl":"https://doi.org/10.18196/jfaps.v3i1.15569","url":null,"abstract":"Cengkir Mango (Mangifera indica L) is a variety of mango known in the northern part of West Java and widely used as food, beverage, and natural-based pharmaceutical product. This fruit is potentially used as body scrub ingredients due to its richness in polyphenols contain. This study aims to formulate natural-based body scrub using the Cengkir Mango as an active ingredient. The formulation was divided into 3 groups with different concentrations of mango fruit flour, respectively 35, 46, and 50 grams (F1, F2, and F3). The product evaluation included homogeneity, stability, pH, and hedonic test. The organoleptic of body scrub indicated mango scent and light brownish-darker colors, responding to an increase of concentration for each formulation. The homogeneity test showed that the body scrub had no granulation of mango flour. The evaluation of pH showed the product had pH respectively at 7.5, 7.5, and 7.0 (F1, F2, and F3). These results indicated that the body scrub had good physical properties. Investigation of body scrub stability was used to evaluate the fragrance, color, and consistency of the formulation in storage for 3 weeks at room temperature. The results showed no change in color and consistency but a loss of mango fragrance after 3 weeks. The hedonic test indicated that most participants favored F1 (35 grams of mango flour). Based on these results, the flour of Cengkir mango had good performance as an active ingredient in a natural-based body scrub.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121932934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant effect of Abelmoschus Esculentus against acetaminophen-induced nephrotoxicity: an experimental study 对乙酰氨基酚肾毒性的实验研究

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-06 DOI: 10.18196/jfaps.v3i1.15284

M. Bekdas, Arzu Meyri Yoldas, Ayşegül Danış, S. Duzcu, M. Alışık, A. Çetinkaya, Hüseyin Kocabey, Idris Turel, M. Dilek, Gokce Kaya Dincel

{"title":"Antioxidant effect of Abelmoschus Esculentus against acetaminophen-induced nephrotoxicity: an experimental study","authors":"M. Bekdas, Arzu Meyri Yoldas, Ayşegül Danış, S. Duzcu, M. Alışık, A. Çetinkaya, Hüseyin Kocabey, Idris Turel, M. Dilek, Gokce Kaya Dincel","doi":"10.18196/jfaps.v3i1.15284","DOIUrl":"https://doi.org/10.18196/jfaps.v3i1.15284","url":null,"abstract":"Acetaminophen(APAP) intoxication is an important cause of nephrotoxicity and hepatotoxicity. N-acetylcysteine(NAC) is used in the treatment, but it has some serious side effects. Abelmoschus esculentus(AE) has various benefits as well as antioxidant effects. This study aims to investigate the effect of AE in APAP-induced acute nephrotoxicity. Forty male Wistar rats were divided into five equal groups: Control, AE, APAP, APAP+AE, and APAP+AE+NAC. Significant changes were observed in serum levels of malondialdehyde (MDA), superoxide dismutase (SOD), Neutrophil Gelatinase-Associated Lipocalin(NGAL) and Kidney Injury Molecule-1(KIM-1) after induction with APAP. NGAL and KIM-1 in the AE group remained low compared to those receiving APAP (p=0.022 and p0.001, respectively). When the APAP group was compared with the AE and AE+NAC groups, it was found that even the administration of AE alone significantly decreased NGAL and KIM-1(p=0.036 vs.p=0.029 and p0.001 vs. p0.001, respectively), these results were attributed to the effects of AE on reducing MDA and increasing SOD. Histopathological studies also confirmed these results. These results demonstrated that AE had protective and therapeutic effects on APAP-induced nephrotoxicity. This benefit of AE is due to its antioxidant effect. In addition, AE may also increase the regenerative capacity of the kidney, which APAP reduces.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127440343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobic Activity of IPPU Padang (Ammannia octandra L.f.) Leaves Ethanol Extract against Skin Pathogenic Microbials IPPU巴东(Ammannia octanandra L.f)的抗菌活性叶片乙醇提取物对皮肤病原菌的抑制作用

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-01 DOI: 10.18196/jfaps.v2i2.13505

Muharni Saputri, V. Mierza, N. Putri

{"title":"Antimicrobic Activity of IPPU Padang (Ammannia octandra L.f.) Leaves Ethanol Extract against Skin Pathogenic Microbials","authors":"Muharni Saputri, V. Mierza, N. Putri","doi":"10.18196/jfaps.v2i2.13505","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.13505","url":null,"abstract":"Ippu Padang plant (Ammannia octandra L.f.) belongs to the family of Lythraceae, a hardy plant that can grow to a height of 50 cm. According to previous research, Ippu Padang leaves contain glycosides, alkaloids, flavonoids and tannins. The presence of alkaloid compounds, flavonoids and tannins is predicted to have potential as an antifungal agent against fungi and antibacterial agent. This research used an experimental method. The steps included collecting plant material, plant identification, processing plants into Simplicia powder, phytochemical screening of Simplicia powder, extracting Simplicia by maceration method using ethanol solvent, antifungal and antibacterial activity test using agar diffusion method and determination of inhibition diameter of leaf ethanol extract. The results of phytochemical screening showed that ippu Padang leaves contained secondary metabolites, namely alkaloids, flavonoids, glycosides, anthraquinone glycosides, tannins, saponins and steroids. The results also revealed that the concentration with the largest inhibitory diameter was 400 mg/ml, namely 12.4 mm against the Candida albicans, 17.46 mm against the Dermacoccus nishinomiyaensis bacteria, 18.53 mm against the Micrococcus luteus bacteria, 19.38 mm against the Pseudomonas aeruginosa bacteria, and 17.71 mm against Staphylococcus epidermidis bacteria. It was concluded that the ethanol extract of ippu Padang leaves could inhibit the growth of the Candida albicans, the bacteria Dermacoccus nishinomiyaensis, Micrococcus luteus, Pseudomonas aeruginosa and Staphylococcus epidermidis.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126008956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant Activity Evaluation from Tomatoes’ N-Hexane, Ethyl Asetate, and Water Fraction with DPPH 用DPPH评价番茄正己烷、乙酸乙酯和水馏分的抗氧化活性

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-03-07 DOI: 10.18196/jfaps.v2i2.13023

R. Dewi, D. Permatasari, Tatiana Siska Wardani, Muladi Putra Mahardika

{"title":"Antioxidant Activity Evaluation from Tomatoes’ N-Hexane, Ethyl Asetate, and Water Fraction with DPPH","authors":"R. Dewi, D. Permatasari, Tatiana Siska Wardani, Muladi Putra Mahardika","doi":"10.18196/jfaps.v2i2.13023","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.13023","url":null,"abstract":"Antioxidants are compounds that can stabilize free radicals in the body. Free radicals are highly reactive molecules as they have unpaired electrons to interact with body cell molecules. Tomatoes contain flavonoids, saponins, solanine tannins, folic acid, malic acid, citric acid, protein, fat, vitamins, minerals, and histamine, which can be used as antioxidants. This study aims to evaluate the antioxidant activity of n-hexane, ethyl acetate, water fraction, and ethanol extracts of Tomatoes and to determine the greatest antioxidant activity between n-hexane, ethyl acetate, water and vitamin C. Tomatoes (Lycopersicum esculentum Mill.) was extracted using the maceration method with ethanol followed by fractionation using n-hexane and ethyl acetate solvents. The test of antioxidant activity to DPPH radical was conducted on n-hexane, ethyl acetate, water, and vitamin C. The antioxidant activity results, expressed by IC50 value to the n-hexane, ethyl acetate, water fraction of Tomatoes fruit, were 4.4603 ppm; 4.0868 ppm; and 4.0527 ppm, respectively. Thus, the greatest antioxidant activity was the water fraction.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"98 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115516730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bunium incrassatum Bois. Batt. Trab. (Talghouda) in the improvement of thyroid tissue damages in female rats 加香布。棉絮。Trab。(Talghouda)在改善雌性大鼠甲状腺组织损伤中的作用

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-03-04 DOI: 10.18196/jfaps.v2i2.13905

M. Aiouaz, A. Bitam

{"title":"Bunium incrassatum Bois. Batt. Trab. (Talghouda) in the improvement of thyroid tissue damages in female rats","authors":"M. Aiouaz, A. Bitam","doi":"10.18196/jfaps.v2i2.13905","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.13905","url":null,"abstract":"This study aims to determine the nutritional and therapeutic effects of dried Bunium incrassatum Bois.Batt.Trab. Tubers powder on rats with hyper and hypothyroidism. Thirty female Wistar rats were divided into 6 groups. G1 (C) received a normal diet. G2 (CT) received a normal diet with dried Talghouda tubers powder dissolved in water. G3 (Hpo) was treated with Carbimazole® (5 mg/day) for 6 weeks. G4 (HpoT) was treated with Carbimazole® (5 mg/day) for 6 weeks and Talghouda for 7 weeks after induced hypothyroidism. G5 (Hpy) was treated with Levothyroxine® (600µg/Kg body weight/day) for 6 weeks. G6 (HpyT) had hyperthyroidism induced by Levothyroxine® (600µg/Kg body weight/day) for 6 weeks and then treated with Talghouda for 7 weeks. A dose of Talghouda was chosen at 1.03g/Kg body weight/day, and all treatments were given by gavage. Phytochemical analysis was carried out for Talghouda, and the thyroid glands of each group were then recovered for histological study. Talghouda treatment showed a highly significant (p 0.01) increase in body weight in groups G2, G4 and G6 with gland repair and reactivation of thyroid follicles in hyper and hypothyroid rats compared to G1, G3 and G5. The powder of dried Talghouda tubers, used as traditional medicine in Algeria, improved the damages caused by hyper and hypothyroidism.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"abs/2202.03866 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124060026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1