Journal of Fundamental and Applied Pharmaceutical Science最新文献_第2页

Hematological Parameters in Subchronic Toxicity Test of Black Garlic Ethanol Extract in Rats 黑蒜乙醇提取物对大鼠亚慢性毒性试验血液学参数的影响

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-02-22 DOI: 10.18196/jfaps.v2i2.13755

Ellen Stephanie Rumaseuw, Y. Iskandar, E. Halimah

{"title":"Hematological Parameters in Subchronic Toxicity Test of Black Garlic Ethanol Extract in Rats","authors":"Ellen Stephanie Rumaseuw, Y. Iskandar, E. Halimah","doi":"10.18196/jfaps.v2i2.13755","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.13755","url":null,"abstract":"The community has used black garlic since ancient times for hypercholesterolemic. Until now, people still consume both raw and cooked black garlic. Black garlic is included in processed garlic products. People on the Asian continent have used black garlic for the past 10 years. This study aims to determine the sub-chronic toxic effect of black garlic ethanol extract on male and female white rats using hematological parameters consisting of hematocrit, hemoglobin, erythrocytes, leukocytes, platelets, MCV, MCH, and MCHC. This research method was a completely randomized design with the administration of ethanolic extract of black garlic to rats in 5 treatment groups, including a negative control group, a dose group of 1000 mg/kg BW, a dose group of 2000 mg/kg BW, a negative control satellite group and a satellite group with a dose of 2000 mg/kg BW. Data were analyzed statistically using one-way ANOVA with a 95% confidence level and SPSS version 20. The study results of black garlic ethanol extract showed no significant effect or were at normal levels on hematological parameters. Therefore, it can be concluded that the ethanolic extract of black garlic is safe for human use as a treatment for hypercholesterolemia.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"214 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124222119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison Between The Suspension and Capsule Preparation from Waste of Avocado Seeds As Antidiarrhea in Induced Mouse 牛油果废籽悬浮液与胶囊制剂对小鼠止泻作用的比较

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-02-21 DOI: 10.18196/jfaps.v2i2.13504

Muharni Saputri, N. Zebua, Fivi Nur Asih, Ghera Fakhira Putri

{"title":"Comparison Between The Suspension and Capsule Preparation from Waste of Avocado Seeds As Antidiarrhea in Induced Mouse","authors":"Muharni Saputri, N. Zebua, Fivi Nur Asih, Ghera Fakhira Putri","doi":"10.18196/jfaps.v2i2.13504","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.13504","url":null,"abstract":"Diarrhea is one type of disease with the most sufferers every year. Thus, it is considered an endemic disease in Indonesia and the potential disease of Extraordinary Events accompanied by mortality. One of the plants that can be used as a traditional medicine for diarrhea is the avocado seed, as it contains tannins, alkaloids, flavonoids, steroids and glycosides, which act as antidiarrheals. This study aims to determine the antidiarrheal effect of avocado seed extract suspension on mice induced by Oleum ricini and the optimum concentration of avocado seed extract suspension and capsules with an antidiarrheal effect in white male mice induced by Oleum ricini. The dosage forms chosen were suspension and capsules. This study used an experimental method with a test sample of the avocado seed. Avocado seed Simplicia was extracted by the percolation method, then an oral suspension and capsule formulation were made from the avocado seed methanol extract. It was evaluated and tested for its effectiveness with mice to cure diarrhea. Normal data were analyzed by One Way ANOVA and Post Hoc Tuckey Method. The results of this study showed that the administration of a suspension of avocado seed methanol extract at a dose of 800 mg/KgBB had the most optimum effect as an antidiarrheal against white male mice with a stool weight of 0.39 grams and a duration of diarrhea for 74 minutes. Furthermore, the administration of avocado seed extract capsules at a dose of 75 mg/KgBB had the most optimum effect as antidiarrheal against white male mice with Loperamide as a positive control. Therefore, it can be concluded that all suspension formulations and capsules of avocado seed methanol extract met the requirements for preparation evaluation. Suspension and avocado seed methanol extract capsules can cure diarrhea in white male mice.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123962218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of Phytochemical Secondary Metabolites of Muntingia Calabura: a Potential as Hepatoprotector 绿野蒙花植物化学次生代谢物的筛选:一种潜在的护肝药物

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-02-07 DOI: 10.18196/jfaps.v2i2.12364

E. D. Pratiwi, Niluh Puspita Dewi

{"title":"Screening of Phytochemical Secondary Metabolites of Muntingia Calabura: a Potential as Hepatoprotector","authors":"E. D. Pratiwi, Niluh Puspita Dewi","doi":"10.18196/jfaps.v2i2.12364","DOIUrl":"https://doi.org/10.18196/jfaps.v2i2.12364","url":null,"abstract":"Muntingia calabura is one of the plants employed to produce herbal-based treatments. Muntingia calabura leaves are traditionally used as an alternative medicine due to their secondary metabolites. The maceration method extracted Muntingia calabura leaves using 96% ethanol solvent for 3 x 24 hours. The fractionation process was carried out using a separating funnel method with different polarities, such as n-hexane, ethyl acetate, and ethanol-water. Thin-layer chromatography (TLC) was used to confirm the phytochemical screening. TLC conditions under UV light 254 and 366 nm using solvents, such as chloroform: methanol (alkaloids), butanol: acetic acid: water (flavonoids), chloroform:methanol: water (saponins), and chloroform: methanol (phenolic). The phytochemical screening results of extracts and Muntingia calabura fractions contained secondary metabolites, such as alkaloids, flavonoids, tannins, saponins, and phenolics. TLC results showed that n-hexane fraction contained flavonoid and saponin compounds; ethyl acetate fraction contained alkaloids, flavonoids, saponins and phenolic compounds; and ethanol-water fraction contained alkaloids, flavonoids, saponins, and phenolics. Muntingia calabura leaves indicated the potential as herbal medicine by containing secondary metabolites.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121484966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Analgesic Activities of Ethanolic and Methanolic Extract of Ganitri Leaves (Elaeocarpus ganitrus Roxb): In vivo study 藤叶乙醇和甲醇提取物的镇痛作用:体内研究

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2021-08-30 DOI: 10.18196/jfaps.v2i1.12159

Naelaz Zukhruf Wakhidatul Kiromah, Chondrosuro Miyarso, Yayu Krisdiyanti

{"title":"Analgesic Activities of Ethanolic and Methanolic Extract of Ganitri Leaves (Elaeocarpus ganitrus Roxb): In vivo study","authors":"Naelaz Zukhruf Wakhidatul Kiromah, Chondrosuro Miyarso, Yayu Krisdiyanti","doi":"10.18196/jfaps.v2i1.12159","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.12159","url":null,"abstract":"Pain is a feeling of discomfort caused by intense or destructive stimuli, which can affect your daily routine if left untreated. Pain can be treated with an analgesic. One of the plants that are considered to have analgesic effects is the leaves of the ganitri. This research aims to determine ethanol and methanol extracts' impact on decreasing analgesic activity and percent protection. The study began with collecting and processing the leaves of the ganitri into the ethanol and methanol extracts using the maceration method. The research was continued with in vivo analgesic activity testing of 24 mice induced by pain using 1% acetic acid. The induced mice were divided into eight treatment groups, where the mice in the first group served as a negative control group. In that group, they were given CMC at a dose of 0.5%. The second group was positive control, given mefenamic acid at a dose of 500 mg/kg BW. In contrast, the third until eighth groups were given ethanol and methanolic extracts of the ganitri leaves with consecutive doses of 100 mg/kg BW, 200 mg/kg BW dan 400 mg/kg BW. Parameters measuring the effectiveness of the extracts used in this study included the amount of stretching, the percentage of analgesic power, and analgesic effectiveness. The results showed that the ethanolic and methanolic extract had the highest percentage of analgesic power at 400 mg/Kg BW amounted to 91,3% and 88,3%. Furthermore, based on the statistical analysis results using ANOVA, it was found that the ethanol and methanolic extracts of the leaves of the ganitri dosage of 400 mg/Kg BW had analgesic activity close to 500 mg/kg BW of mefenamic acid.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128883987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antimicrobial Test of 1-(2.5-Dihydroxi Phenyl)-(3-Pyridine-2-Il) -Propanone Compound in Enterococcus Faecalis and Escherichia Coli Bacteria Using a Well Diffusion Method 孔扩散法测定1-(2.5-二氢苯基)-(3-吡啶-2- il)-丙烷复合物对粪肠球菌和大肠杆菌的抑菌作用

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2021-02-26 DOI: 10.18196/JFAPS.V1I2.10983

A. Wibowo, Rivaldy Rifai Hatala, Awalludin M Edang

{"title":"Antimicrobial Test of 1-(2.5-Dihydroxi Phenyl)-(3-Pyridine-2-Il) -Propanone Compound in Enterococcus Faecalis and Escherichia Coli Bacteria Using a Well Diffusion Method","authors":"A. Wibowo, Rivaldy Rifai Hatala, Awalludin M Edang","doi":"10.18196/JFAPS.V1I2.10983","DOIUrl":"https://doi.org/10.18196/JFAPS.V1I2.10983","url":null,"abstract":"1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound is a compound synthesized by reacting the pyridine-2-carbaldehyde and 2.5-dihydroxyacetophenone compound without solvent with K2CO3 (Potassium Carbonate) catalyst in the microwave. The 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound is a chalcone derivative compound substituted by two hydroxy groups on ring A and has 2-pyridyl groups on ring B. Chalcone is a secondary metabolite compound from the flavonoid group, which has several activities as anti-platelet, anti-bacterial, immunomodulator, anti-hyperglycemic, and anti-inflammatory. This study aims to determine the antibacterial effect of 1-(2.5-dihydroxifenil)-(3-pyridine-2-il)-propenone compound against Enterococcus faecalis and Escherichia coli bacteria. This study used TLC (Thin Layer Chromatography) and Melting Point Test to analyze the purity of 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound. Meanwhile, the test for antibacterial activity used a well diffusion method. Concentration variation for 1-(2.5-dihydroxifenyl)-(3-pyridine-2-il)-propenone compound as antibacterial in Escherichia coli were 0.25 mg/100 μl, 0.5 mg/200 μl, and 0.75 mg/300 μl. Meanwhile, the concentration variation for Enterococcus faecalis bacteria was 5%, 2.5%, 1.25% and was replicated three times. The results of the compound purity test using the melting point test and Thin Layer Chromatography (TCL) showed that the 1-(2.5-dihydroxy phenyl)-(3-pyridine-2-il)-propenone compound was pure. The results of the antibacterial activity test for 1-(2.5-dihydroxiphenyl)-(3-pyridine-2-il)-propenone compound showed no zone of inhibition at each test concentration. In conclusion, the 1-(2.5-dihydroxifenyl)-(3-pyridine-2-il)-propenone compound did not have an antibacterial effect on Enterococcus faecalis and Escherichia coli bacteria.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"281 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131508067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clove Oil (Syzygium aromaticum) Edible Film Formulation and Antibacterial Activity Test against Streptococcus mutans 丁香油(Syzygium aromaticum)食膜配方及对变形链球菌的抑菌活性试验

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 1900-01-01 DOI: 10.18196/jfaps.v2i1.11640

W. Ningsih, Afdhil Arel

{"title":"Clove Oil (Syzygium aromaticum) Edible Film Formulation and Antibacterial Activity Test against Streptococcus mutans","authors":"W. Ningsih, Afdhil Arel","doi":"10.18196/jfaps.v2i1.11640","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.11640","url":null,"abstract":"Clove oil contains eugenol as an antibacterial. Meanwhile, products containing clove oil have been widely used as toothpaste and mouthwash. In this study, clove oil was formulated in the form of edible film because it is practical, easy to use, and could be used without water like other oral hygiene preparations. The edible film is a thin layer film made of consumable materials used as a carrier of antibacterial compounds. Clove oil edible film was then formulated with clove oil concentrations of 1%, 1.5%, and 2% and determined for its antimicrobial activity against Streptococcus mutans. Clove oil edible film preparations were evaluated under their physical properties, including friability, drying shrinkage, pH, thickness, and swelling ability. Antibacterial activity testing of clove oil edible film was conducted, employing the blood agar diffusion method against Streptococcus mutans. The physical evaluation of the clove oil edible film showed almost the same physical properties as the comparison (GF). Clove oil edible film test results revealed the greatest inhibition at F1 of 18.6 mm ± 0.577, F2 of 22.3 mm ± 2.081, and F3 of 25.3 mm ± 1.527. According to David and Stout, the inhibition activity of bacteria on F3 was categorized as a very strong group inhibition response. In addition, ANOVA test analysis results uncovered that the concentration of clove oil affected the inhibition of the Streptococcus mutans bacteria with a significance value of 0.000 (p 0.05). Also, Duncan's test exhibited that each concentration of clove oil had a significant difference in the inhibition of Streptococcus mutans bacteria.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"75 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124175485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hair Tonic Formulation of Black Tea Extract (Camellia sinensis) as Hair Growth 红茶提取物(茶树)对头发生长的护发配方

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 1900-01-01 DOI: 10.18196/jfaps.v2i1.12451

Nurul Arfiyanti Yusuf, Besse Hardianti, R. Rahma

{"title":"Hair Tonic Formulation of Black Tea Extract (Camellia sinensis) as Hair Growth","authors":"Nurul Arfiyanti Yusuf, Besse Hardianti, R. Rahma","doi":"10.18196/jfaps.v2i1.12451","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.12451","url":null,"abstract":"Black tea contains flavonoid and its derivate which is a mechanism of actions to stimulate and increase cell proliferation of hair dermal papilla cell to suppress the production of tumor necrosis factor alpha (TNF alpha) that trigger baldness. This study aimed to determine the stable hair tonic formula and to investigate the effectiveness of black tea extract hair tonic in rabbits (oryctolagus cuniculus) as animal testing. There were 3 different concentrations used in this study namely formula 1 (1.25 % of black tea extract), formula 2 (2.5 % of black tea extract) and formula 3 (5 % of black tea extract). The physical stability evaluation was conducted using some tests such as organoleptic, pH, density determination and viscosity test. The result of this evaluation showed that all the three formulas were stable, its effectiveness as hair restorer then was tested and the result of this test indicated that black tea extract has activity as a hair fertilizer and the optimum extract was shown by the formula 3 with black tea extract concentration of 5% with the hair growth in the 18th day i.e., 9.62 ± 0.365 mm.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131309362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Formulation and Optimization of Furosemide Snedds With Variation Concentration of Tween 80 and PEG 400 随吐温80和PEG 400浓度变化的速尿注射剂的配方及优化

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 1900-01-01 DOI: 10.18196/jfaps.v2i1.12180

Sesilia Putri Nandita, I. Kuncahyo, Reslely Harjanti

{"title":"Formulation and Optimization of Furosemide Snedds With Variation Concentration of Tween 80 and PEG 400","authors":"Sesilia Putri Nandita, I. Kuncahyo, Reslely Harjanti","doi":"10.18196/jfaps.v2i1.12180","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.12180","url":null,"abstract":"Furosemide is a potent diuretic drug that has low bioavailability. Furosemide can be formulated into nanoemulsion preparations using the SNEDDS method to increase its bioavailability as SNEDDS can form stable nanoemulsions with droplet sizes 200 nm. This study aims to identify the optimum formula for variations in the concentration of surfactant Tween 80 and cosurfactant PEG 400 based on the characterization tests of emulsification time, percent transmittance, and drug loading. The independent variables used in this study were Tween 80 and PEG 400. Seven furosemide SNEDDS formulas from the Simplex Lattice Design (SLD) method were tested for characterization in the form of emulsification time, percent transmittance, and drug loading. The characterization results were optimized using Simplex Lattice Design. The optimum formula was re-characterized, including emulsification time, percent transmittance, drug loading, particle size, zeta potential, and in vitro dissolution. The results were then compared with theoretical values and analyzed using the One-Sample T-test method. Optimization results showed Tween of 61.4922% and PEG 400 of 18.5078% with the characterization of emulsification time 15.25 seconds, percentage transmittance 94.20%, drug loading 50 100.2 ppm, particle size 12.18 nm. Furthermore, the zeta potential was -17.6 mV, and the in vitro dissolution rate reached 106.71% within 15 minutes.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"110 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130643562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Qualitative and Quantitative Analysis of Dexamethasone in Rheumatic Pain Herbal Medicine Using Thin-Layer Chromatography (TLC) – Densitometry 用薄层色谱-密度法定性定量分析风湿镇痛中草药中地塞米松的含量

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 1900-01-01 DOI: 10.18196/jfaps.v2i1.12450

D. Permatasari, Novita Kurniasri, Muladi Putra Mahardika

{"title":"Qualitative and Quantitative Analysis of Dexamethasone in Rheumatic Pain Herbal Medicine Using Thin-Layer Chromatography (TLC) – Densitometry","authors":"D. Permatasari, Novita Kurniasri, Muladi Putra Mahardika","doi":"10.18196/jfaps.v2i1.12450","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.12450","url":null,"abstract":"Chemical medicine is chemical substances that is used as the main ingredient of chemical drugs. This compound is usually added to herbal medicine preparation to strengthen the indication of the traditional medicine.Chemical medicine was prohibited from being added to traditional medicinal preparations. But in reality, a lot of medicinal herbs could have contained medicinal chemicals on the market sale. This purpose of this research was to know the chemical contamination of the dexamethasone also the concentration contained in the rheumatic pain herbal medicine. This research was used three kind of rheumatic pain herbal medicine sample to identify dexamethasone, the sample code is S1, S2, and S3. The analysis of qualitative method are organoleptic test, FTIR characteristic test, TLC evaluation. The analysis of quantitative were purposed to know the dexamethasone concentration contained on the rheumatic pain herbal medicine using TLC-Densitometric method. The result show that the three sample of rheumathic pain herbal medicine were contaminated by dexamethasone chemical medicine. Based on organoleptic test, the results show color, smell, and taste. Characterization of the dexamethasone using FTIR were to know functional groups of dexamethasone contained in the rheumatic pain herbal medicine sample, the functional groups of the sample S1, S2, and S3 were compared to dexamethasone standard. To identify of TLC method, obtained Rf value of dexamethasone standard and the sample, visualizing a stain color purple-fluorescence on the UV 254 nm. The analysis of quantitative dexamethasone concentration using TLC-Densitometric showed the presence of dexamethasone in the sample for S1, S2 and S3. The concetration of dexamethasone obtained of sample S1, S2, S3 were 1014.64 µg/g ; 131.15 µg/g ; 135.54 µg/g respectively.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114669329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis 异三角蜂蜂胶非极性部分次生代谢物鉴定及抗菌活性研究

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 1900-01-01 DOI: 10.18196/jfaps.v2i1.12406

A. Aziz, Veggy Nadya Yuliawan, P. M. Kustiawan

{"title":"Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis","authors":"A. Aziz, Veggy Nadya Yuliawan, P. M. Kustiawan","doi":"10.18196/jfaps.v2i1.12406","DOIUrl":"https://doi.org/10.18196/jfaps.v2i1.12406","url":null,"abstract":"Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2 mm ± 1.5) at 200µg/mL concentration.","PeriodicalId":270723,"journal":{"name":"Journal of Fundamental and Applied Pharmaceutical Science","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133819364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0