Total Flavonoid Content of Lemongrass Leaf (Cymbogoncitratus (DC.) Stapf) Extract and Antioxidant Activity with Frap

Journal of Fundamental and Applied Pharmaceutical Science Pub Date : 2022-08-31 DOI:10.18196/jfaps.v3i1.15556

Ahda Maulida Ulufan Nurinnafi’a, Kusumaningtyas Siwi Artini, D. Permatasari

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引用次数: 1

Abstract

Lemongrass (Cymbogoncitratus (DC.) Stapf) leaves contain alkaloids, saponins, tannins, anthraquinones, steroids, phenols and flavonoids. Flavonoids act as antioxidants as they can reduce free radicals. This study aims to determine the total flavonoid content, antioxidant activity, and IC50 value of lemongrass leaf extract (Cymbogoncitratus (DC.) Stapf). Extracts were made by maceration using 96% ethanol as solvent. Testing of total flavonoid content with the AlCl3 method using UV-Vis spectrophotometry was carried out three times. The antioxidant activity test used the FRAP (ferric reducing antioxidant power) method on extracts containing 100, 200, 300, 400, and 500 ppm. The test results showed that the leaf extract of citronella (Cymbogoncitratus (DC.) Stapf) had a total flavonoid content of 22,60 mg QE/g extract. Furthermore, there was antioxidant activity in the leaf extract of lemongrass (Cymbogoncitratus (DC.) Stapf indicated by the formation of a blue color purplish when reacted with FRAP solution, and IC50 extract value was 71,59 ppm and included in the category of strong antioxidants.

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柠檬香茅叶总黄酮含量测定葡萄叶提取物及其抗氧化活性研究

柠檬草(Cymbogoncitratus, DC.)葡萄叶含有生物碱、皂苷、单宁、蒽醌、类固醇、酚类和类黄酮。类黄酮可以作为抗氧化剂，因为它们可以减少自由基。本研究旨在测定香茅叶提取物(Cymbogoncitratus, DC.)的总黄酮含量、抗氧化活性及IC50值。Stapf)。以96%乙醇为溶剂浸渍制得提取物。用紫外-可见分光光度法测定三次总黄酮含量。抗氧化活性测试采用FRAP(铁还原抗氧化力)法对含有100,200,300,400和500ppm的提取物进行测试。实验结果表明，香茅叶提取物(Cymbogoncitratus, DC;Stapf)的总黄酮含量为22,60 mg QE/g提取物。柠檬草(Cymbogoncitratus, DC.)叶提取物具有抗氧化活性。Stapf与FRAP溶液反应后呈蓝色略带紫色，萃取物IC50值为71,59 ppm，属于强抗氧化剂范畴。

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Journal of Fundamental and Applied Pharmaceutical Science

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