SHAKEEL AHMED TUNIO, Muhammad Naeem, ATIQUE AHMED BEHAN, ASMATULLAH KAKA, HUMA RIZWANA, Nasir Rajput, NOOR UN NISA MARI
{"title":"Phenotypic Characterization of Indigenous Kachhi Sheep Breed","authors":"SHAKEEL AHMED TUNIO, Muhammad Naeem, ATIQUE AHMED BEHAN, ASMATULLAH KAKA, HUMA RIZWANA, Nasir Rajput, NOOR UN NISA MARI","doi":"10.57038/usjas.v6i04.5838","DOIUrl":"https://doi.org/10.57038/usjas.v6i04.5838","url":null,"abstract":"For the evaluation of the phenotypic and morphometric characteristics of Kachhi sheep, a total of 300 adult Kachhi sheep (female240 and male 60) were selected from different study areas (Hyderabad, Matiary, Mirpurkahs, Sanghar, Umerkot and Thaparkar) of Sindh province. Among all the study areas majority of male and female Kachhi sheep physically possessed plain white coat with black and brown (tan) color, compact body shape, coarse type wool, convex face profile, roman nose, small pendulous ears, sloppy rump, dark colored hooves, and small cylindrical tail, compact udder. Moreover, majority of male and female sheep were noted with wattles, remarkably few cases of female and male Kachhi sheep were detected with deviated Morphometric characters. Average live body weight and linear body measurements (chest girth, body length, height at withers, facial length, ear length, head width and tail length) of both sexes, at Matiary area were observed comparatively (P<0.05) higher, followed by Hyderabad, Sanghar, Mirpurkhas, Umerkot and Tharparkar vicinities of Sindh. However, statistical (P<0.05) differences were recorded in the morphometric traits between male and female Kachhi sheep among all the selected vicinities of Sindh province.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125351719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
OYERONKE ADENIKE ADEKOLA, AKINWALE DAMI OLABIMISI, GBOLAGADE BENJAMIN ADESIJI, SOLA EMMANUEL KOMOLAFE
{"title":"Plant-Based Treatment of Helminths in Calves","authors":"OYERONKE ADENIKE ADEKOLA, AKINWALE DAMI OLABIMISI, GBOLAGADE BENJAMIN ADESIJI, SOLA EMMANUEL KOMOLAFE","doi":"10.57038/usjas.v6i04.5834","DOIUrl":"https://doi.org/10.57038/usjas.v6i04.5834","url":null,"abstract":"This study examines the indigenous plant practices for the treatment of helminths in cattle among settled agro-pastoralist in Ogun and Oyo State, Nigeria. The study was a pretest-posttest design. Three sets of helminth-infected cattle were each exposed to herbal treatment (Indigenous plant concoction (Group A, n=6), alverticine drug (convectional helminthic drug (Group B, n=6) orally for 1-14 days while the control group (Group C, n=6) were untreated. Faecal egg count was obtained using the Modified Mac Master and saturated salt solution technique and data were analyzed using ANOVA for repeated measures. The significant effect of treatment on FEC count across the four days of treatment (p < 0.05) as the FEC count significantly decline across from day 1 - 4 for both the herbal than the conventional treatment groups while the control group remains chronically infected with the helminths till 12th week. FEC count significantly decline faster for convectional drug group (FEC count cleared on 2nd day) compared to the herbal group (FEC count cleared on 4th day) (p < 0.05). Cows exposed to herbal drug significantly (90.36±4.780) had better weight gain than those exposed to conventional treatment (85.65±7.405) while there was significant weight loss observed in the control group (76.68 ±6.435) at the 12th week. The study concludes that herbal concoctions based traditional knowledge of medicinal plants were efficacious in the treatment of helminths infestation. It is therefore recommended that regular control measure should be practiced and agro-pastoralist needed to be educated in proper usage of anti-helminths drugs and its administration.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"357 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133009384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MAHEEN KHAN, MUHAMMAD FARHANULLAH KHAN, AMJAD SULTAN, GHAZALA YASMEEN
{"title":"Efficacy of Conventional Insecticides Against Maize Stem Borer (Chilo Partellus) and Shoot Fly (Atherigona Soccata)","authors":"MAHEEN KHAN, MUHAMMAD FARHANULLAH KHAN, AMJAD SULTAN, GHAZALA YASMEEN","doi":"10.57038/usjas.v6i03.5212","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5212","url":null,"abstract":"Maize stem borer, Chilo partellus Swinhoe and shoot fly, Atherigona soccata Rondani are considered as major pests of maize (Zea mays, L.). Carbofuran (Carbamate), Endosulfan (Organochlorine), Phorate (Organophosphate), Permethrin (Pyrethroid) and two neonicotinoid insecticides; Imidacloprid and Thiamethoxam were selected to compare their efficacy against Chilo partellus and Atherigona soccata on maize crop under natural infestation during spring, winter and autumn seasons. All insecticides reduced Atherigona soccata and Chilo partellus infestation significantly (p<0.05). Imidacloprid and thiamethoxam were found statistically at par against Atherigona soccata and better than endosulfan. Permethrin and carbofuran were found statistically at par in first week and better than phorate while in second week permethrin and carbofuran were statistically at par but phorate was found better than them.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122115962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Managing Ducks for Better Production in Ahmed Ecofarm, Keshurbari, under the District of Dinajpur, Bangladesh","authors":"ASHRAFUL KABIR","doi":"10.57038/usjas.v6i03.5204","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5204","url":null,"abstract":"Duck farming is not going equal to chicken farming in Bangladesh. Due to some superstitions, many people do not consume ducks’ meat and egg. In this regard, this article will provide those positive pieces of information about successful duck farming to consume its meat and eggs. Being a riverine country, Bangladesh is suitable for rearing ducks, and natural feed is available. Khaki Campbell (laying breed) and Beijing (table breed) were kept as two study birds for qualitative analyses through the questionnaire method. The result suggested that 2-months aged per duck consumed 70 g feed and 6-months adult duck 140 g daily with the cost of 3.22 and 6.44 taka. The semi-intensive farm provided 336 kg of regular feed for 4000 ducks (3200 Khaki Campbell, 800 Beijing duck). 1000 square feet brooding room maintained 30-32oC temperature and 100 watts (60 pieces) bulbs and 200 watts (30 pieces). For egg-laying, only sand was used (100%). After sexing the day-old ducklings, 30% found male ducks during the rearing period. The owner provided snails and hyacinth occasionally with the conventional feed. Ahmed Eco- farm was established only for egg purposes, so out of 3200 Khaki Campbell, 400 ducks laid eggs daily (50-60%) when they reached 150 days with a bodyweight of 1.5 kg. Viral paralysis, kidney disease, and flu were the diseases, and vaccines, antibiotics, and overall hygiene helped control that situation. Ahmed Ecofarm in Dinajpur district is in profit by supplying eggs in the local markets. This study period was from January 2020 to April 2022.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117155321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MUHAMMAD ABDUL MANAN, MARYAM SHERAZI, AMBER NAEEM, LAIBA SHAFIQUE
{"title":"Study on the Immunity Interventions A Deterrent Effect Against Pathogens","authors":"MUHAMMAD ABDUL MANAN, MARYAM SHERAZI, AMBER NAEEM, LAIBA SHAFIQUE","doi":"10.57038/usjas.v6i03.5202","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5202","url":null,"abstract":"The year 2020 is exceptional year in human history and normal activities have almost stopped. That indicates human life will face greater consequences of Pandemics (COVID-19) in future. Few decades back man was not highly dependent on science and technology as today and has become ease oriented that pushing us toward non-healthy lifestyle. Economic challenges, social pressures, and health issues are some of things that might negatively affect our immune system. The cytokine storm was decreased by a large dosage of vitamin C, which also lowered chances of ICU admission and moderate or mild lymphopenia recovery in people who slept well by enhancing immunity mechanisms. Weak immune system is a big threat and life can be miserable if we failed to improve, revive and replenish our immune system. Healthy and active life style can increase immunity which will give us hope to fight with these types of catastrophic conditions.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117190795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ariana Ali, Israr Maqbool, Muhammad Ashraf, TASLEEM KAUSAR, FAISAL JAMEE, MALJA IMAN YOUSAF, Saima Talib, Alia Hussain
{"title":"Effects of Feed Additives Garlic and Onion on Growth of Mrigal (Cirrhinus Mirgala) Fingerlings","authors":"Ariana Ali, Israr Maqbool, Muhammad Ashraf, TASLEEM KAUSAR, FAISAL JAMEE, MALJA IMAN YOUSAF, Saima Talib, Alia Hussain","doi":"10.57038/usjas.v6i03.5205","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5205","url":null,"abstract":"The purpose of this study was to see how dietary garlic and onion powder effected on Cirrhinus mrigala. Feed additives such as garlic and onion are used. Cirrhinus mrigala was fed garlic and onion supplementary meals containing 2.5 percent of their body weight for a total of 60 days. The initial body weight and length of Cirrhinus mrigala fingerlings were respectively 18.48±0.139g and 10.17±0.123cm. When garlic and onion were fed together, the effect of garlic and onion on Cirrhinus mrigala growth performance was examined. Final weight was 29.37±0.071 and final length was 13.2±0.022cm, indicating that feed consumption was enhanced based on growth performance. The current findings showed that dietary garlic or onion powder, given alone or in combination, might increase mrigal (Cirrhinus mrigala) survival, growth, and feed consumption. Based on the findings, it can be concluded that feeding a food supplement (onion and garlic) to fish, either alone or in combination, enhanced their growth performance.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126315036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
BUSHRA RASHEED, MUHAMMAD IMRAN BIRMANI, ROMANA ARSHAD
{"title":"Biodiversity and Distribution of Fish Fauna in Dera Ghazi Khan Canal","authors":"BUSHRA RASHEED, MUHAMMAD IMRAN BIRMANI, ROMANA ARSHAD","doi":"10.57038/usjas.v6i03.5209","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5209","url":null,"abstract":"The DG Khan canal is a vast canal starting from Taunsa Barrage and flowing through different areas of the DG Khan District. In some places, its water is restricted and has many fish species. The present study was intended to examine the fish fauna of DG Khan district from the Taunsa dam at DG Canal to the boundary line of DG Khan District with Rajanpur District. During the study, 16 species including Gudusia chapra (Hamilton, 1822), Notopterus notopterus (Pallas, 1769), Labeo gonius (Hamilton, 1822), Labeo rohita (Hamilton, 1822), Cirrhinus mrigala (Bloch, 1795) , Catla catla (Hamilton, 1822), Puntius ticto (Hamilton, 1822), Puntius sophore (Hamilton, 1822), Securicula gora (Hamilton, 1822), Cyprinus carpio (Linnaeus, 1758), Mystus cavasius (Hamilton, 1822), Mystus bleekeri (Day, 1877), Wallago attu (Day, 1878), Channa marulia (Hamilton, 1822), Oreochromis niloticus (Linnaeus, 1758), Mastacembelus armatus (Lacepède, 1800), were recorded in the DG canal of DG khan district. Taunsa barrage was also explored in which fishes Chitala chitala (Hamilton, 1822), Catla catla (Hamilton, 1822), Labeo gonius (Hamilton, 1822), Labeo rohita (Hamilton, 1822), Cirrhinus mrigala (Bloch, 1795), Labeo calbasu, Labeo bogga (Hamilton, 1822), Labeo dyocheilus (McClelland, 1839), Cirrhinus reba (Hamilton, 1822), Labeo boggut (Sykes, 1839), Hypophthalmichthys molitrix (Valenciennes, 1844), Ctenopharyngodon Idella (Valenciennes, 1844), Cyprinus carpio (Linnaeus, 1758), Oreochromis mossambicus (Peters, 1852), Oreochromis aureus (Steindachner, 1864), Oreochromis niloticus (Linnaeus, 1758), Channa punctata (Bloch, 1793), Channa striata (Bloch, 1793), Channa marulius (Hamilton, 1822), Macrognathus pancalus (Hamilton, 1822), Mastacembelus armatus (Lacepède, 1800), Ompok bimaculatus (Bloch, 1794) , Wallago attu (Day, 1878), Sperata seenghala (Sykes, 1839), Eutropiicthys vacha (Hamilton, 1822), Bagarius bagarius (Hamilton, 1822), Rita rita (Hamilton, 1822), Goanolisa manminus and Rita macracanthus (Ng, 2004) were recorded.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132668166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
IRFAN BABOO, MUHAMMAD JANSHAIR, KHALID JAVED IQBAL, Muhammad Shahbaz, AHSAN SHAFIQ1, HAFIZ MUHAMMAD SAJAWAL
{"title":"Behavioural Responses and Feeding Preference of Hemiechinus Collaris ( Eulipotyphla: Erinaceidae)","authors":"IRFAN BABOO, MUHAMMAD JANSHAIR, KHALID JAVED IQBAL, Muhammad Shahbaz, AHSAN SHAFIQ1, HAFIZ MUHAMMAD SAJAWAL","doi":"10.57038/usjas.v6i03.5210","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5210","url":null,"abstract":"The behavioral aspects of Hedgehog (Hemiechinus collaris) kept in Nocturnal Captive, Nocturnal free range, diurnal captive, and diurnal free-range conditions were recorded and compared with each other. The behavior aspects such as Jumping, aggressiveness, digging, stress, rolling up, immovability, walking, standing, drinking, lying and feeding were observed. Non-significant differences were observed in jumping and feeding behavior of all four types of conditions. Similarly, aggressiveness, digging, stress, roll up, immovability, walking, standing, drinking, and lying behaviors show significant differences in Nocturnal Captive, Nocturnal free range, diurnal captive and diurnal free-range condition. Different feeds such as natural feed, bakery waste, meat waste, and tea stall waste were offered to H. collaris which were arranged in four groups (G-I, G-II, G-III and G-IV). Natural feed consumed constantly by animals of all groups and non-significantly difference observed in percentages of consumed feed. The maximum bakery waste feed consumed by animals of Group-I significantly differed from the other three groups G-II, G-III and G-IV, while percentage of consumed meat waste feed recorded highest in G-I which significantly differed from other groups. The fourth type of feed was tea stall waste which was consumed highest by H. collaris which was kept in G-IV and also shows significant difference with animals of G-I, G-II and G-III.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"139 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123632988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Emergence of the ABC Transporter Antibiotic Resistant Gene in Pathogenic Fungi Using In Silico Analysis","authors":"FATIMA ISMAIL, EQAZA JAVED, IQRA AHMAD","doi":"10.57038/usjas.v6i03.5211","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5211","url":null,"abstract":"Adenosine triphosphate binding cassette proteins (ATP-ABC) transporters belong to a broad superfamily of proteins in all living organisms that have essential physiological functions. They are necessary for transporting a wide range of substrates across lipid membranes. ABC transport proteins share two conserved domain architectures: the multiple transmembrane helices (TMD) nucleotide binding protein domain (NBD). Fungi have evolved active transport pathways through endogenous and exogenous toxicants. Drug efflux from ABC transporters lowers the concentration of intracellular drugs, in many pathogens and cancer cells; therefore, efflux is subjected to extensive studies in pathogens. ABC transporter gene of aspergillus versicolor as reference and evaluated metadata of ABC transporter gene in comparison from dermatophyte and non-dermatophyte fungi using fungi DB and NCBI databases. ABC transporters gene was studied using gene homology via phylogenetic analysis. Study evaluated ABC transporters sub families, number of transcripts, exon, intron numbers sizes and position using gene sequence analysis by NCBI. The evolutionary history of ABC gene analyzed by using 12 proteins of both dermatophytes. Study also explored the single nucleotide polymorphism (SNPs) homology in dermatophytes using the NCBI SNP tool. ABC protein examined five sub-families which include ABC-B, ABC-C, ABC-D, ABC-F. Phylogenetic studies on ABC transporter genes showed its emergence in three major groups of dermatophytes and non- dermatophyte. Exon number of ABC transporter gene varies from 2 to 18 and one transcript number in dermatophytes and non-dermatophytes. The evolutionary history of ABC-B was analyzed by using 12 proteins of both dermatophytes and non-dermatophytes. ABC genes in dermatophytes were found in five subfamilies ABC-B, ABC-C, ABC-D, ABC-F and ABC-G. However, ABC transporter gene family survey suggested that ABC- C family is a highly emerged sub family in dermatophytes and non-dermatophytes. Study revealed that ABC protein has been found in five sub-families of ABC transporter gene in dermatophytes. It has one transcript number in dermatophytes and non-dermatophytes. Whereas, the exon numbers are varied from 2 to 18. Moreover, ABC transporter gene phylogenetic family survey suggested that ABC- C family is highly emerged in both fungal types.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131146979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABDUL SAMAD MEMON, ASMATULLAH KAKA, ABDULLAH CHANNO, MUHAMMAD BAKHSH, QUDRATULLAH KALWAR, ABDULLAH SETHAR
{"title":"Effect of Soybean Based Extenders on Milk and Tris Egg Yolk in Tharparkar Bull","authors":"ABDUL SAMAD MEMON, ASMATULLAH KAKA, ABDULLAH CHANNO, MUHAMMAD BAKHSH, QUDRATULLAH KALWAR, ABDULLAH SETHAR","doi":"10.57038/usjas.v6i03.5208","DOIUrl":"https://doi.org/10.57038/usjas.v6i03.5208","url":null,"abstract":"About 40 (n=10) ejaculates were taken from four Tharparkar Bulls (A, B, C, D) having age of 4-5 years, after the collection, ejaculates were examined for initial evaluation i.e. volume, color, pH, motility, wave motion, viability, morphology, membrane integrity and sperm concentration. Ejaculate having ≥ 70% motility, morphology, membrane integrity and viability were processed and extended into Tris based extender (Control) and Soyabean extender with 3, 6, 9, 12, 15ml concentration of soybean milk. frozen thawed samples were evaluated for motility, morphology, viability and membrane integrity. samples diluted in tris extender resulted in48.50±1.2 motility, 65.00±1.5 morphology, 67.25±3.4 viability and 63.75±2.9 membrane integrity. However, In Soyabean based extender at the quantity of9 ml frozen samples improved semen quality parameters 57.75±1.1 motility, 73.00±3.4 morphology, 72.25±0.3 viability and 70.25±1.3 membrane integrity. After lab-based evaluation, samples diluted with Tris and optimized concentration of 9 ml of Soyabean based extender were inseminated into 20 cows (n=10 each group). Pregnancy was followed after 60 days of AI (Artificial Insemination) Semen diluted with Soya bean-based extender resulted in improved conception rate up to 60%. It was concluded that the soyabean based extender improved the cooled and frozen thawed semen quality of Tharparkar bull semen.","PeriodicalId":265691,"journal":{"name":"University of Sindh Journal of Animal Sciences (USJAS)","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122095756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}