Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering最新文献

{"title":"ACCUMULATION OF CR6+, PB2+ AND CD2+ AND ULTRAVIOLET RADIATION ALTER METHYLATION AND GENOMIC DNA STATUS IN RAMALINA FARINACEAE","authors":"R. Hamutoğlu, D. Cansaran Duman, M. Derici, Sümer Aras, Alican Aslan","doi":"10.53447/COMMUNC.915250","DOIUrl":"https://doi.org/10.53447/COMMUNC.915250","url":null,"abstract":"In this study was aimed to determine the genotoxic effect of Ramalina farinacea lichen species against stress sources at the molecular level. After applying three different heavy metals (Pb2+, Cd2+, and Cr6+) to the R. farinacea, the extent to which the lichen sample absorbed these metals was determined by Flame Atomic Absorption Spectroscopy. RAPD and MSAP-AFLP assays were also used to determine the status of DNA damage. The heavy metal analysis showed that R. farinacea had high levels of Pb2+, Cd2+, and Cr6+ content. According to the results obtained from molecular analyses, band changes were observed against seven primers heavy metal stresses and three primers against UV stress. An increase in Genomic Template Stability (GTS) was determined during the time in R. farinacea treated with all heavy metal concentrations. The effect of UV radiations in R. farinacea revealed the highest polymorphism and the lowest GTS rate depending on the dose. Among all methylation combinations, Type II was found to show altered in R. farinacea in response to Pb2+, Cd2+, and Cr6+ contents and UV radiations. R. farinacea can be used at the molecular level as a biomarker of suitable genotoxic effect. This is the first study to reveal DNA damage against stress sources using a sample of R. farinacea lichen species.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121747774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTS OF LIGHT AND SALINITY ON THE GERMINATION OF CLOSELY RELATED THREE SALSOLA TAXA","authors":"İ. Çinar, G. Tug","doi":"10.53447/communc.1034430","DOIUrl":"https://doi.org/10.53447/communc.1034430","url":null,"abstract":"Three closely related Salsola taxa (Salsola boissieri Botsch. subsp. serpentinicola (Freitag & Özhatay) Freitag & Uotila, Salsola boissieri Botsch. subsp. boissieri, Salsola turcica Yıldırımlı (halophytic ecotype), Salsola turcica Yıldırımlı (gypsicole ecotype)) from different edaphic conditions were studied according to changing light and salinity conditions. Seeds of target taxa were collected in 2017 and their weights were determined. The perianth segments were removed before the experimental trials and all the trials were conducted at 9°C/22°C which is the mean night and day temperatures of germination season. For the determination of the influence of light, one set of seeds for distilled water trial were kept at complete darkness. Different NaCl concentrations (distilled water, 100, 200, 300 mM NaCl) were used to evaluate the effects of salinity on germination. Viability of the seeds were determined by Triphenyl Tetrazolium Chloride (TTC) test which was applied to the seeds that did not germinate during the trials. As a result, it was found out that light stimulates germination of the taxa and Salsola seeds showed better germination ratio at light. The most tolerant taxa against salinity are the halophytic and gypsicole ecotypes of S. turcica, and the most susceptible one is S. boissieri subsp. serpentinicola. Both of the species show reduced germination ratios with increasing salinity. Salinity tolerance of S. boissieri subsp. serpentinicola and S. boissieri subsp. boissieri are very low, according to the Decreasing Germination Percentage (DGP) values. Although they show different germination response against increasing salinity, there is not any statistically meaningful difference between these three taxa according to germination percentages at different salinities, germination rates, last germination ratios and seed viabilities (F=1.818 p>0.05) (One Way ANOVA, SPSS 25).","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116900699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HIGHLIGHTING THE MELISSOPALYNOLOGICAL AND PHYSICOCHEMICAL CHARACTERISTICS OF AYDER-RİZE (TURKEY)","authors":"O. Çelemli̇, Aslı Özkök, Ç. Özenirler, Nazlı Mayda, Golshan Zare, K. Sorkun","doi":"10.53447/communc.906788","DOIUrl":"https://doi.org/10.53447/communc.906788","url":null,"abstract":"The aim of this research is characterizing the honey produced in Ayder/Rize-Turkey. In this context 20 honey samples were collected from Ayder in 2018. The melissopalynological and physicochemical analysis of the honey samples were done by this research. According to the melissopalynological analysis 40 taxa belong to the 22 plant families were identified as botanical sources of the investigated honey samples. Nine of the investigated samples (sample no 1-9) were evaluated as monofloral and the others as multifloral honeys. As a result of melissopalynological analysis, while pollen belong to the Castanea sativa taxon were found in dominant ratios in some of the investigated samples, the pollen belongs to the Castanea sativa, Trifolium repens, Lotus corniculatus, Coronilla orientalis taxa were found as secondary in some other honey samples. Total pollen number in 10 gram honey (TPN 10) values of the samples were found between 7 732 and 167 147 by melissopalynological analysis. The first step of the physicochemical analsyis was the moisture analysis and the values found between 15,8% and 18,8% (mean value:7,01±0,98 %). The fructose/glucose analysis were done by High Performance Liquid Chromatography (HPLC) and the values for fructose found as: 26,43-35,57g/100g (mean: 31,39±2,41 g/100g), glucose values as; 20,11-30,58 g/100g (mean: 26,83±2,16 g/100g). Also fructose/glucose ratio was found as 1,03-1,34 (mean: 1,17±0,10). Hydroxymethylfurfural (HMF) and total phenolic acids analysis were done also by HPLC. The results for HMF analysis is between 0,7- 11,31 ppm (mean: 3,8±2,6 ppm ), for total phenolic acids the value are found between 88,92±0,04 mgGAE/kg - 196,17±0,10 mgGAE/kg (mean: 121,98±0,1 mgGAE/kg). As a step of chemical analysis; the volatile compounds of the honey samples were determined by Gas Chromatography and Mass Spectrometry (GC-MS). The compounds belong to the aldehydes, aliphatic acids and esters, alcohols, hydrocarbons, carboxylic acids and their esters, ketones, terpenes, fatty acids and their esters groups were found as a result of GC-MS analysis. The compounds belong to the carboxylic acids and their esters, fatty acids and their esters identified in higher ratios compare to the other compounds. Since, there is little detailed published information about the quality and properties of Ayder-Rize honey, the current study aims to characterize the honey belong to the this region.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123893157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IN SILICO PROOFS FOR PHLORIDZIN, NARINGENIN, AND CINNAMIC ACID AS ALPHA-AMYLASE ACTIVATORS, WHICH IS IMPORTANT IN INDUSTRIAL MICROBIOLOGY OR BIOCHEMICAL ENGINEERING","authors":"Ergin Murat Altuner","doi":"10.53447/communc.934706","DOIUrl":"https://doi.org/10.53447/communc.934706","url":null,"abstract":"Enzymes are commonly defined as biological catalysts, regulating particular biochemical reactions. α-Amylase (EC 3.2.1.1) is one of the industrially important enzymes, which are extensively used in starch hydrolyzing processes, such as brewing, fermentation, detergent production, food processing, etc. This enzyme breaks down α-1,4 glycosidic bonds in amylose or amylopectin. The end products from amylose are maltotriose and maltose. Maltose, glucose, and limit dextrin are formed from amylopectin. There are many studies in the literature regarding the α-amylase inhibitors, which have the potentials of being used in diabetes and obesity. However, there is a very limited number of studies in the literature about the activation of this enzyme, which could be harmful to such diseases. This study aims to support the activation activity of phloridzin, naringenin, and cinnamic acid for α-amylase, which was previously proved experimentally, with some in silico tests.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130274397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NEW RARE MACROPHYTE RECORD FROM SW ASIA AND ECOLOGICAL CONDITIONS AT ITS HABITAT","authors":"A. Yaprak, Selçuk Tuğrul Körüklü, G. Tug","doi":"10.53447/communc.1023578","DOIUrl":"https://doi.org/10.53447/communc.1023578","url":null,"abstract":"Aldrovanda vesiculosa L. is a cosmopolitan but very rare carnivorous aquatic angiosperm species from the family Droseraceae. The species was widespread and more common few decades ago but unfortunately it is being extinct in so many former distribution areas due to aquatic pollution and eutrophication. During the field trip to Karakuyu Lake we recognized a remarkable free-floating plant, after detailed investigation we identified the specimens as Aldrovanda vesiculosa which is a new record from Turkey and also SW Asia. In this publication, we provided detailed description of the species based on Turkish specimens, its habitat description, water physiochemical attributes, co-occurring macrophyte species list and botanical illustration of the species.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131229827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ALTERATIONS OF ALDOSE REDUCTASE ACTIVITY BY INDOLE-3-CARBOXALDEHYDE DERIVATIVES","authors":"Orçun Erdem Kurşun, S. Süzen, Ö. Yildirim","doi":"10.53447/communc.798355","DOIUrl":"https://doi.org/10.53447/communc.798355","url":null,"abstract":"In diabetic conditions, aldose reductase (AR, EC 1.1.1.21) activity is significantly increased in lens, kidney and nerve tissues. Mainly in ocular and neural tissues increased levels of sorbitol is associated with diabetic complications such as retinopathy, nephropathy, neuropahty, cataract formation and also tissue damage via increased reactive oxygen speices. Recently, there are many studies that show the relationship between this enzyme family and cancer. Aldose reductase is important for the pathway and in turn has been a potential target for drug design. Great number of aldose reductase inhibitors (ARIs) are used for prevention or delay of these diabetic complications and cancer. However, effective ARIs which has benefits in diabetic complications are still under investigation. In this study aldose reductase was partially purified from bovine lens and the inhibitory effects of 16 different indol-3-carboxyaldehydederivatives on aldose reductase enzyme activities were examined by kinetic assays. These results suggested that N'-[(5-bromo-1H-indol-3-yl)methylidene]pyridine-4-carbohydrazide showed that the highest inhibitory activity on AR.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115788610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BIOCHEMICAL AND PATHOLOGICAL EFFECTS ON THE MALE RAT HEPATIC TISSUE AFTER EXPOSURE TO 900MHZ ELECTROMAGNETIC FIELD DURING ADOLESCENT PERIOD","authors":"Ayşe İkinci Keleş, H. S. Erol, T. Sapmaz, T. Mercantepe, G. Keleş, Burcu BİTERGE SÜT, E. Odacı, M. Halici, S. Polat","doi":"10.53447/communc.764890","DOIUrl":"https://doi.org/10.53447/communc.764890","url":null,"abstract":"The aim of this study was to employ biochemical analysis and histopathological techniques to investigate the effects of the application of a 900-megahertz electromagnetic field during adolescence on the rat liver. 24 male Sprague Dawley rats aged 21 days were randomly divided into electromagnetic field, sham and control groups. No procedure was performed on the control group rats. Electromagnetic field group rats were exposed to a 900-megahertz electromagnetic field in an electromagnetic field application cage (1 hours/day, every day for 25 days). The sham group rats were placed in the same electromagnetic field cage without exposure to electromagnetic field. At the end of the experiments, the livers were removed. The livers were used for histopathological evaluation (light, electron and immunofluorescence microscopy) and biochemical analyses. Biochemical analyses revealed increased lipid peroxidation and glutathione values while catalase and superoxide dismutase values were decreased in electromagnetic field group. Electron microscopy evaluations identified necrotic hepatocytes with numerous cytoplasmic and mitochondrial vacuoles in electromagnetic field group sections. We also observed an enlarged endoplasmic reticulum and loss of mitochondrial matrix, in addition to the presence of vacuoles. Also, loss of inner and outer mitochondrial membrane integrity was observed. Light microscopy evaluations revealed sinusoidal capillary dilatation and degeneration in hepatocytes with cytoplasmic swelling, vacuolar degeneration and pyknotic nuclei in electromagnetic field group sections. Deterioration in the integrity of the collagen fibers was also present around the vena centralis. Additionally, electromagnetic field had no effect on body weight. Therefore, we conclude that continuous 900-megahertz electromagnetic field treatment may cause changes in oxidative stress biomarkers and the morphology of the adolescent rat liver.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133318002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CHARACTERIZATION OF ITS1 SECONDARY STRUCTURE IN TEN SPECIES OF COLUTEOCARPEAE (BRASSICACEAE) AND ITS TAXONOMICAL UTILITY","authors":"Kurtulus Özgisi","doi":"10.53447/communc.820287","DOIUrl":"https://doi.org/10.53447/communc.820287","url":null,"abstract":"Utility of the internal transcribed spacers (ITSs) of ribosomal RNA sequences to infer phylogenetic relationships among organisms have been proven. Although ITS1 and ITS2 are highly variable in sequence, they have conserved structures that have a key function in the processing of rRNA gene transcripts. Determining of such a conserved motif can help to identify relationships between organisms. Since ITS2 has much more conserved secondary structure, structural properties of ITS1 are generally neglected by researchers. In this study, ITS1 secondary structures of ten representative species, which were once assigned under different genera, of tribe Coluteocarpeae were determined. Also taxonomical utility of ITS1 secondary structure was also tested. Analyses indicate that there are four different types (4-, 6-, 7- and 8 hairpin) of secondary structures. On the other hand, each sequences have a conserved region that is common among land plants. Since previous studies reveals other species, that belong different tribes or lineages of Brassicaceae show similar ITS1 secondary structure, it is not a useful delimitation tool for investigated species in terms of taxonomy.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"115 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121360259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TAXONOMIC CONTRIBUTIONS TO THE GENUS DIANTHUS (CARYOPHYLLACEAE) FROM TURKEY","authors":"E. Hamzaoğlu","doi":"10.53447/communc.882759","DOIUrl":"https://doi.org/10.53447/communc.882759","url":null,"abstract":"According to the related literature and some taxonomic websites, it was proven that Dianthus liboschitzianus and D. erinaceus (var. erinaceus) are the valid names, which are accepted as a synonym. It was determined that Dianthus erinaceus var. alpinus not D. erinaceus (var. erinaceus) is a taxon, which is a synonym under Dianthus webbianus. Furthermore, the lectotypes were determined for Dianthus erinaceus and D. masmenaeus var. glabrescens, which was typed by being based on more than one specimen.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"2019 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125861109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"THE NIN-LIKE PROTEIN (NLP) FAMILY IN COMMON BEAN: GENOME-WIDE IDENTIFICATION, EVOLUTION AND EXPRESSION ANALYSIS","authors":"I. Büyük, Aybüke Okay, T. Aksoy, Sümer Aras","doi":"10.53447/communc.869501","DOIUrl":"https://doi.org/10.53447/communc.869501","url":null,"abstract":"One of the plant-specific transcription factor families that play an important role in responses to nitrogen deficiency is NODULE INCEPTION-like (NIN-like) proteins (NLPs). However, the properties and evolutionary relationships of NIN genes in P. vulgaris, which enable nodule formation naturally, have not been studied yet. 12 Pvul-NIN genes have been identified in this study and the approximate positions of these genes have been determined. At the same time, several biochemical and physicochemical properties of NIN-like proteins have been elucidated. Comparisons between both monocot and dicot, but also nodule binding and non-nodule binding species were considered when investigating the evolutionary relationships of NIN genes. 16 duplication events (14 segmental and 2 tandem) have been shown to play a role in the expansion of the NIN gene family in P. vulgaris. In addition, comparative expression analysis of NIN genes was performed by processing publicly available RNAseq data and different levels of Pvul-NIN gene expression under both salt and drought stress were detected, suggesting the roles of Pvul-NIN gene for abiotic stress response. Expression levels of NIN genes have also been investigated in different plant tissues and have been shown to be intensely expressed in nodules and root tissues. This is the first study on the in-silico detection and characterization of Pvul-NIN genes to examine gene expression levels in common bean. The results could therefore provide the basis for future studies of functional characterization of Pvul-NIN genes.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131835340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}