Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering最新文献

Detection target genes in combating biofilm forms in $textit{Salmonella}$ Typhimurium 14028 检测抗击$textit{Salmonella}$ Typhimurium 14028中生物膜形式的目标基因

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-07-15 DOI: 10.53447/communc.1317798

Sarah Farhad Ni̇had, N. Akçelik, M. Akçelik

{"title":"Detection target genes in combating biofilm forms in $textit{Salmonella}$ Typhimurium 14028","authors":"Sarah Farhad Ni̇had, N. Akçelik, M. Akçelik","doi":"10.53447/communc.1317798","DOIUrl":"https://doi.org/10.53447/communc.1317798","url":null,"abstract":"In this study, the relationship of $textit{hilA, invA, adrA, spiC, otsB}$ and $textit{csgD}$ genes, which are known to play critical roles in the pathogenicity and virulence of $textit{Salmonella}$ strains, with biofilm formation was investigated by examining the changes in the expression levels of these genes during the transition from planktonic form to biofilm form. When the virulence gene expressions between the S. Typhimurium 14028 mutant, which lost its ability to form biofilms due to $textit{csgD}$ gene deletion, and the wild type strain were compared, it was determined that the expression levels of $textit{hilA, invA}$ and $textit{adrA}$ genes increased, whereas the expression levels of $textit{spiC, otsB}$ and $textit{csgD}$ genes decreased. These data indicate that all examined genes play critical activation or inhibition roles in biofilm regulation as well as pathogenicity and virulence. On the other hand, in the mutant strain; The increase in the expression levels of $textit{hilA, invA}$ and $textit{adrA}$ genes shows that inhibitors of the proteins encoded by these genes have the potential to be of practical use in the prevention and control of infections caused by both biofilm-forming and non-biofilm-forming $textit{Salmonella}$ strains.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139359039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The cytotoxic and apoptotic effects of $textit{Abies nordmanniana subsp. bornmülleriana Mattf }$ resin extract on prostate cancer cell cells $textit{Abies nordmanniana subsp.

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-07-06 DOI: 10.53447/communc.1272043

M. B. Batır, Sevinç Batir, Feyzan OZDAL KURT, Sırrı Çam

{"title":"The cytotoxic and apoptotic effects of $textit{Abies nordmanniana subsp. bornmülleriana Mattf }$ resin extract on prostate cancer cell cells","authors":"M. B. Batır, Sevinç Batir, Feyzan OZDAL KURT, Sırrı Çam","doi":"10.53447/communc.1272043","DOIUrl":"https://doi.org/10.53447/communc.1272043","url":null,"abstract":"Cancer has become one of the most critical health issues, with an increasing incidence and mortality in recent years. Meanwhile, many studies are carried out on discovering new compounds which reflects effective results on cancer cells. Therefore, this study aimed to examine the cytotoxic, anti-proliferative and apoptotic effects on cancer cell lines by using the extract obtained from Uludağ Fir ($textit{Abies nordmanniana subsp. bornmülleriana Mattf }$) resin. Uludağ Fir resin extract was obtained by applying the methanol extraction method. In our study, fibroblast cell L-929, human metastatic prostate cancer PC-3 and human metastatic prostate cancer DU-145 cell lines were cultured in RPMI-1640 medium. The effects of the resin extract concentrations on the viability of the cells were determined with the Muse™ Cell Count & Viability test. In addition, apoptosis and cell cycle phases of the cells were determined using the Muse™ cell analyzer. Finally, RT-qPCR analysis was performed to determine the resin extract effect on pro-apoptotic and anti-apoptotic gene expression. The apoptotic effect of Uludağ Fir resin extract on cancer cell lines was significantly higher in contrast to the healthy normal cells. In addition, it was determined that Uludağ Fir extract caused G0/G1 cell cycle arrest in cancer cells. In RT-qPCR analysis, the pro-apoptotic gene expression levels were apparently increased in cancer cells, which was followed by a decrease in the anti-apoptotic gene expression levels. All taken together, these results indicate that Uludağ Fir resin extract exerts two prong effects as it induces a cell cycle arrest and apoptotic pathway activation on human prostate cancer cell lines.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139362179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper(II) bioremoval by thermophile Cyanobacterium aponinum 嗜热菌aponinum对铜(II)的生物去除

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1135560

Seda Şen, Nur KOÇBERBER KILIÇ, G. Dönmez

{"title":"Copper(II) bioremoval by thermophile Cyanobacterium aponinum","authors":"Seda Şen, Nur KOÇBERBER KILIÇ, G. Dönmez","doi":"10.53447/communc.1135560","DOIUrl":"https://doi.org/10.53447/communc.1135560","url":null,"abstract":"In the current study, bioremediation of Cu(II) by thermophile Cyanobacterium aponinum has been studied in BG11 media under different conditions. The optimum pH was 9 due to the maximum Cu(II) bioremoval efficiency as 71% in the medium with12.8 mg/L Cu(II). According to the results obtained from the trials, the highest bioremoval was 76.6% in the medium including 9.7 mg/L Cu(II) for incubation period of 10 days. When the effect of increasing temperature (25-45 °C) and biomass [20% and 40% (v/v)] concentrations on bioremediation by C. aponinum was investigated, the highest heavy metal removal was found 75.8% at 45 °C, 12.8 mg/L Cu(II), and 20% (v/v) biomass concentration. It was 76.3% in the medium with 13.8 mg/L pollutant, 40% (v/v) biomass concentration. The qm (maximum specific Cu(II) removal) was found as 6.1 mg/g at 45 °C in BG11 with 40% (v/v) biomass and 13.8 mg/L Cu(II). It has been concluded that Cu(II) bioremediation by thermophile C. aponinum was firstly investigated at various environmental conditions in this study. The results indicated that the tested cyanobacterium had a great potential to remove heavy metals from the aquatic environments, containing Cu(II).","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114390645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of the macro anatomy of the Hazel Dormouse’s (Muscardinus avellanarius Linnaeus, 1758) (Rodentia: Mammalia) skeletal system in Türkiye 云南榛睡鼠(Muscardinus avellanarius Linnaeus, 1758)(啮齿目:哺乳类)骨骼系统的宏观解剖学评价

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1254635

Esra ENGİN KİLİC, Hatice MUTLU EYİSON, Erkut Ki̇vanc

引用次数: 0

New record of exotic land snail Rumina decollate (Linnaeus, 1758) in Iraq 伊拉克外来蜗牛Rumina decollate新记录(Linnaeus, 1758)

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1202072

Ragda H. AL-QAİSİ, K. Farman

引用次数: 0

The evaluation of the growth performance of a cyanobacterial isolate Phormidium lucidum (Kützing ex Gomont 1892) grown under different environmental conditions and its use as a dietary source for Daphnia magna (Straus 1820) 不同环境条件下生长的蓝细菌分离株紫藻(Phormidium lucidum, k<s:1> tzing ex Gomont 1892)生长性能的评价及其作为大水蚤(Daphnia magna)饲料来源的应用(Straus 1820)

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1241869

K. Karakaş, K. M. Eryalçın, M. Ergönül, T. Atıcı, S. Atasağun

{"title":"The evaluation of the growth performance of a cyanobacterial isolate Phormidium lucidum (Kützing ex Gomont 1892) grown under different environmental conditions and its use as a dietary source for Daphnia magna (Straus 1820)","authors":"K. Karakaş, K. M. Eryalçın, M. Ergönül, T. Atıcı, S. Atasağun","doi":"10.53447/communc.1241869","DOIUrl":"https://doi.org/10.53447/communc.1241869","url":null,"abstract":"This study presents the effect of cyanobacterium isolated from Bolluk Lake (Konya, Türkiye) which is a saline lake on the growth performance of Daphnia magna. Isolated cyanobacteria species were identified as Phormidium lucidum according to its 16S rDNA sequences. The effects of different growth conditions including pH (7.18, 8.15, 9.17 and 10.26), light intensity (1200, 2400, 3600 and 4800 lux), temperature (10, 20, 25 and 30°C) and nitrogen concentrations (0.25, 0.5, 1.0 and 1.5 g/L) on P. lucidum was studied. Effects of each environmental factor on biochemical composition (total protein, total lipid and chlorophyll-a concentration) of P. lucidum were also studied. The optimum growth conditions were found as pH 7.18, ambient temperature 20°C, nitrogen 0.25 g/L and light intensity 3600 lux, after a 2-week incubation period. The effects of various mixtures of the cyanobacteria and Chlorella vulgaris which is a common feed for Daphniids were also evaluated for their effects on the growth rates of D. magna. The best growth rate for D. magna was obtained in the medium containing 100% P. lucidum at the end of the 13ᵗ ͪ day.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"303 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123201249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual dimorphism in the lacertid lizard Apathya cappadocica (Werner, 1902) (Reptilia: Lacertidae) from southeastern Türkiye 来自斯里兰卡东南部的长尾蜥(爬虫纲:长尾蜥科)的两性二态性

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1185771

K. Candan, E. Yıldırım, Y. Kumlutaş, Osman Sinan Güni̇ster, Ç. Ilgaz

{"title":"Sexual dimorphism in the lacertid lizard Apathya cappadocica (Werner, 1902) (Reptilia: Lacertidae) from southeastern Türkiye","authors":"K. Candan, E. Yıldırım, Y. Kumlutaş, Osman Sinan Güni̇ster, Ç. Ilgaz","doi":"10.53447/communc.1185771","DOIUrl":"https://doi.org/10.53447/communc.1185771","url":null,"abstract":"Apathya cappadocica is a medium-sized lizard species included in the family Lacertidae. The species is known from Türkiye, Northern Syria, Iraq, and Western Iran. Sexual dimorphism (SD), which is a phenomenon including phenotypic differences between males and females, has many effects on behaviour, shape and size characteristics. A total of 87 adult lizard specimens collected from south-eastern Anatolia were used in this study. The results of ANOVA showed that all morphometric features exhibited a pattern in which males have larger size than females. According to principal component analysis (PCA), the first three factors explain 81.553% of the total variance. Differences in head size between sexes have been well-documented in lizards and are associated with male-male aggression which results in mating success. This kind of pattern is supported by this study. Additionally, the results showed that males have more femoral pores, which is a signalling mechanism for sexual selection, than females. In conclusion, it can be accepted that the species in the genus Apathya has a similar pattern in accordance with the larger male theory.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121699109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of lead (Pb) on the growth rates of two aquatic macrophyte species; Limnobium laevigatum (Humb & Bonpl. ex Willd) Heine and Egeria densa Planch. grown in different experimental media 铅对两种水生植物生长速率的影响海棠(Humb & Bonpl)海涅和埃格里亚稠密的普兰奇。在不同的实验培养基中生长

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1225993

F. Di̇kmen, Vahide Cansu Seymenoğlu, M. Ergönül

{"title":"The effect of lead (Pb) on the growth rates of two aquatic macrophyte species; Limnobium laevigatum (Humb & Bonpl. ex Willd) Heine and Egeria densa Planch. grown in different experimental media","authors":"F. Di̇kmen, Vahide Cansu Seymenoğlu, M. Ergönül","doi":"10.53447/communc.1225993","DOIUrl":"https://doi.org/10.53447/communc.1225993","url":null,"abstract":"Anthropogenic causes contribute to toxic pollutants in aquatic environments and heavy metal pollution. As a heavy metal, Lead (Pb), is one of the most common causes of pollution in water. Heavy metals must be removed from the aquatic environment because they adversely affect health and all living things in each environment. In this study we aimed to determine the effects of lead (Pb) exposure on the growth rates and biomass of two aquatic macrophyte species, E. densa and L. laevigatum. Plants grown in in two different experimental media. For this purpose, both plants were exposed to 3 different concentrations of lead (1 ppm, 5 ppm, 15 ppm). Samples were measured on the 1st, 4th and 7th days, and the first and last weights of the plants were compared. Bioexperiments were run in triplicate. Positive values were observed in the growth rates of both plants, except for the negative growth rates observed on the 1st day at 1 ppm and 5 ppm lead concentrations in the pond water environment. Both plants showed positive growth in 25% Hoagland medium at all concentrations and days, except for the 1 ppm lead concentration, being observed for E. densa. As a result of our study, lead exposure did not significantly alter the growth rates of E. densa and L. laevigatum in the experimental media used for short-term (up tp 7 days) durations.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117312710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential 海洋假单胞菌测定铅(II)生物传感器电位的研究

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2023-06-03 DOI: 10.53447/communc.1184290

C. Bostanci, H. Nazır, G. Dönmez

{"title":"An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential","authors":"C. Bostanci, H. Nazır, G. Dönmez","doi":"10.53447/communc.1184290","DOIUrl":"https://doi.org/10.53447/communc.1184290","url":null,"abstract":"Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode (SPE) carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the Pb(II) solution for electrochemical analysis. After the exposure time, cyclic voltammetry (CV) and differential pulse voltammetry (DPV) analyses were carried out. The study shows that the sensor was found in a linear range between 1x10−8 M and 8x10−8 M, with the lowest detection limit 10−9 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mV/s, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"88 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128001698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of melatonin on rat soleus muscle treated with carbon tetrachloride 褪黑素对四氯化碳处理大鼠比目鱼肌的影响

Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering Pub Date : 2022-12-30 DOI: 10.53447/communc.1178452

Duygu Fevziye Vural, Hatice MUTLU EYİSON, D. Özerkan, S. Cebesoy

{"title":"The effect of melatonin on rat soleus muscle treated with carbon tetrachloride","authors":"Duygu Fevziye Vural, Hatice MUTLU EYİSON, D. Özerkan, S. Cebesoy","doi":"10.53447/communc.1178452","DOIUrl":"https://doi.org/10.53447/communc.1178452","url":null,"abstract":"Antioxidants are known to restrains various tissue damage caused by the administration of carbon tetrachloride (CCl4). This study examined whether melatonin (MEL), a molecule known to have antioxidant properties, has a protective effect on the rat soleus muscle, where toxic damage is caused by the application of CCl4. In the study, eighteen albino-type male Wistar rats were used and divided into three groups as Control Group (group 1), CCl4 group (group 2) and CCl4 + MEL group (group 3). End of the 12 weeks, blood samples were taken as intracardiac from the rats under ketamine/rompun anesthesia, and the soleus muscles of the rats were removed. Tissue samples were subjected to routine preparation procedures for light microscopy. Sections 5 µm thick taken and stained with hematoxylin-eosin (HE) for histopathological examinations and Masson’s Trichrome stain for fibrosis formations. In conclusion, the CCl4 group displayed muscular hypertrophy, fiber orientation dysfunction and atrophy in some areas. In addition, fibrosis was spotted around the venous and nerve plexuses. In contrast to the CCl4 group, the melatonin group displayed no fibrosis and maintained tissue integrity. Therefore, when comparing CCl4+MEL and CCl4 groups, it was observed that melatonin had a stabilizing or even curative effect on the injuries.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"461 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127532923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0