{"title":"Histochemical effects of brodifacoum on rat spleen","authors":"Burcu BAYRAMLI ÖNER, N. Gül","doi":"10.53447/communc.1168968","DOIUrl":"https://doi.org/10.53447/communc.1168968","url":null,"abstract":"In this study, the histochemical effects of Brodifacoum, an anticoagulant used against rodents, on the spleen are examined under a light microscope using CD4 and CD8 histochemical staining methods. A single dose of 0.2 mg Brodifacoum was dissolved in Dimethyl Sulfoxide (DMSO) and was given orally to mature male rats. Spleen samples were collected under ether anesthesia after 24 h, 72 h, 14 days, and 30 days from the rats in the experimental groups and after 14 days from the rats in the control group. In this light microscope study, it was observed that the capsule, white pulp, and red pulp zones in the rat spleen were constructed normally and as their natural structures primary and secondary follicles (germinal center) they were few, and CD4 and CD8 lymphocytes were spherically structured. In the 24 h spleens of the rats, the diameters of germinal centers were expanded and deterioration of the structure of CD4 and CD8 cells was observed. Related to the increase in time (72 h and 14 days) it was determined that primary follicles increased in number and the diameters of germinal centers expanded. In addition to this, after30 days, the rate of CD4:CD8 of the brodifacoum applied rat spleens were approximately the rate of the control group, and the improvement of the structures of the cells was reported as an effect of regeneration. As a result of this study, it was found that Brodifacoum caused immunohistochemical abnormalities in the rat spleen, affected the morphological structure of CD4 and CD8 T lymphocytes and created an immune response in rats. It is thought that the obtained results will be a source for the studies on Brodifacoum.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127260288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Çubuk stream pollution and environmental effects","authors":"T. Atıcı, Didem BALOĞLU ONGAR","doi":"10.53447/communc.1171792","DOIUrl":"https://doi.org/10.53447/communc.1171792","url":null,"abstract":"The water quality parameters of Çubuk Stream were examined in terms of physical and chemical aspects and the pollution level of Çubuk Stream was determined by comparing these parameters with the Surface Water Quality Regulation (SWQR). For this purpose; samples were taken from three different stations between August 2012 and April 2013, the water samples were compared with each other and with previous studies, the water quality of Çubuk Stream was determined according to the surface water quality regulation. In our study, the biological oxygen requirement of Çubuk Stream according to SWQR (BOD5: 22.5mg/l) IV. class, ammonium nitrogen element (NH4–N:2.34 mg/l) IV. class, nitrite nitrogen element (NO2–N: 0.035 mg/l) II. class, nitrate nitrogen element (NO3 –N:0.95 mg/l) Class I, according to phosphate value (PO4:0.075 mg/l) Class I and trace elements (Pb, Cd, Cr, Ni, Mn) values Class I. water quality. According to the ratio of NH4-N/PO4 (2.34/0.075=31.2 mg/l), the element that limits eutrophication in Çubuk Stream is P. Compared to previous studies, after the wastewater treatment plants established in the region, higher quality water is provided, especially in terms of NO3 and PO4. It has been determined that the nitrogen and phosphorus ratios are high because the region, which is dense in terms of organic matter, is close to industrial establishments.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117006894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rediscovery of an ancient herbarium for Turkish botanical history: Bolu Girls Village Institute Herbarium","authors":"Nursel Ikinci","doi":"10.53447/communc.1143523","DOIUrl":"https://doi.org/10.53447/communc.1143523","url":null,"abstract":"Bolu Girls’ Village Institute Herbarium (Bolu Kız Köy Enstitüsü Herbaryumu) was founded in 1953 by Muhittin Fehmi Özgen. It is one of the earliest herbaria of the Turkish Republic. The herbarium was part of the natural history collections of the school which also included insects, minerals, and fossils. The herbarium initially contained around 2500 specimens within 72 cardboard boxes. Additionally, specimens from this herbarium were sent to İstanbul University Department of Biology Herbarium (ISTF) and around 600 specimens to the herbarium of Royal Botanic Garden Edinburgh (E). In 2006, we received 567 plant specimens in five boxes. The plants were mainly collected from Bolu and neighboring provinces between 1953 and 1958. The herbarium collection contains one bryophyte specimen and three seedless vascular plants. The remaining taxa belong to gymnosperms and angiosperms. The herbarium labels were prepared both in Latin and Turkish. Majority of the specimens have identification up to family and genus level and a small number of them up to species level. This herbarium remained largely unknown to Turkish botanists. Therefore, we think it is valuable to provide detailed information about the history and the content of the herbarium. The information given here not only will contribute to our knowledge about the natural history collections of Turkey but also to the education history of the Turkish Republic.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123737149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"COI gene analyses of the Daghestan pine vole (Microtus daghestanicus Shidlovsky, 1919) population from northeastern Turkey","authors":"D. Çetintürk","doi":"10.53447/communc.1133489","DOIUrl":"https://doi.org/10.53447/communc.1133489","url":null,"abstract":"Daghestan pine vole (Microtus daghestanicus Shidlovsky, 1919) is spread in Caucasia, Turkey and Northwestern Iran and distribution of this species is limited to Northeastern Anatolia in Turkey. Few molecular studies on M. daghestanicus have been performed so far, and it was analysed in this study with the mitochondrial cytochrome oxidase subunit I (COI) region and compared with other Terricola species (Microtus subterraneus and Microtus majori) and other Microtus species found in its distribution area (Microtus arvalis and Microtus mystacinus). For this purpose, mean genetic distance values and fixation index values were calculated. Also, Bayesian Inference tree and Median-joining network were constructed. The acquired results showed that M. daghestanicus was clearly separated in the Pleistocene Period and was closer to M subterraneus than M. majori in the subgenus Terricola.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117120866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Peg-stabilized collagen-chitosan matrices: physicochemical and in vitro biological assessment of hydrogels","authors":"Serap Sezer, Y. M. Elçin","doi":"10.53447/communc.1147162","DOIUrl":"https://doi.org/10.53447/communc.1147162","url":null,"abstract":"In this study, some of the physicochemical and in vitro biological properties of hydrogel matrices formed under different crosslinking conditions, consisting of the structural protein collagen and chitosan polysaccharide were investigated. The chemical compositions of the developed matrices were verified, their surface morphologies were examined by SEM and AFM, and their light transmittance status was determined. Next, the in vitro cytocompatibility of the hydrogels was demonstrated based on one week of interaction with mesenchymal stem cells. As a result, highly transparent and cytocompatible hydrogel matrices with mechanical stability in aqueous conditions were obtained.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128286955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First records for benthic macroinvertebrate fauna in Güzeldere Waterfall, Turkey","authors":"N. Arslan, Deniz Mercan","doi":"10.53447/communc.1194182","DOIUrl":"https://doi.org/10.53447/communc.1194182","url":null,"abstract":"In this paper, the benthic macroinvertebrate fauna of the Güzeldere Stream and Waterfall were investigated. Samples were collected in October 2020 at 3 stations. A total of 41 taxa including Gastropoda, Oligochaeta (9 species), Ephemeroptera (7 species), Odonata, Coleoptera, Chironomidae (15 species), Simuliidae, Blephariceridae (2 species), Plecoptera (3 species), and Trichoptera (1 species) were identified in the area. The dominant taxon was Chironomidae at all three stations followed by Oligochaeta and Ephemeroptera, respectively. The second station had the highest individual numbers (195) and the highest species diversity (32). All of the identified taxa were the first records for the study area because there have been no studies conducted for the determination of the Güzeldere Stream and Waterfall.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129272823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An investigation of Rabies virus existence on rodents by using new PCR primer pairs","authors":"Tugce Gunkan, Mehmet Ali Öktem, Ferhat Matur","doi":"10.53447/communc.1062833","DOIUrl":"https://doi.org/10.53447/communc.1062833","url":null,"abstract":"Zoonotic diseases are the infections to be carried between human and other vertebrates. Rhabdoviruses belong to a virus family which could infect a wide range of host organism. It is important to find new molecular diagnostic tools and primers for the identification of the viruses to be able to make the molecular identification process faster and reveal new forms of the virus. Rodents are the primary mammals group that can uncontrollably go in and out from quarantine regions. Therefore, in this study, 242 Apodemus spp. (wood mouse) and Myodes glareolus (bank vole) specimens collected from 16 localities in province Zonguldak, Çaycuma district were used to scan brain tissues to determine RABV using hemi-nested PCR. Also, to determine RABV a new primer pairs were designed using already published sequences. According to results, eight specimens were showed positive bands for RABV. Those eight sequences blasted. But the sequences did not match according to the Blast result. The designed primer pairs provide positive bands on electrophoresis for positive control so that the primer pairs are new and can be used for following studies. With this study it was also tested whether rodents are the potential carriers for RABV since they are primarily prey source for carnivores, and domestic animals.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125337904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Contributions to the flora of Beyağaç (Denizli)","authors":"M. Çiçek, Okan Çon","doi":"10.53447/communc.1128722","DOIUrl":"https://doi.org/10.53447/communc.1128722","url":null,"abstract":"In this study, in order to determine the flora of Beyağaç (Denizli), 767 plant samples were collected and observed from 74 different localities by the field studies carried out in Beyağaç and its close surroundings in the years 2017 and 2018. The results of examination of the collected plant samples and evaluation of the observed plants, it was determined 362 species and totally 363 plant taxa, belonging to 63 families and 228 genera. Of 363 vascular plant taxa, 3 taxa belong to Pteridophyta division, 360 taxa to Magnoliophyta division. Of the Magnoliophyta division, 4 taxa belong to Gymnospermae subdivision and 356 taxa to Angiospermae subdivision. Of the Angiospermae subdivision, 312 taxa belong to Dicotyledonae classis and 44 taxa to Monocotyledonae classis. The phytogeographical spectrum of the flora of Beyağaç (Denizli) as follows: Mediterranean elements 115 taxa (31.6%), Irano-Turanian elements 13 taxa (3.6%), Euro-Siberian elements 20 taxa (5.5%) and multi-regional or unknown origin 216 taxa (59.3%). The largest ten families in flora of Beyağaç (Denizli) as follows: Fabaceae 43 taxa (11.8%), Asteraceae 42 taxa (11.5%), Lamiaceae 28 taxa (7.7%), Caryophyllaceae 27 taxa (% 7.4), Brassicaceae 24 taxa (6.6%), Poaceae 16 taxa (4.4%), Boraginaceae 14 taxa (3.8%), Apiaceae 13 taxa (3.6%), Asparagaceae 11 taxa (3.0%), and Plantaginaceae 10 taxa (2.7%). The largest ten families constitute 64% of the flora of Beyağaç (Denizli). The largest ten genera in the flora of Beyağaç (Denizli) as follows: Alyssum L. 8 taxa (2.2%), Silene L. 7 taxa (1.9%), Medicago L. 6 taxa (1.6%), Salvia L. 6 taxa (1.6%), Cerastium L. 5 taxa (1.4%), Euphorbia L. 5 taxa (1.4%), Muscari Mill. 5 taxa (1.4%), Teucrium L. 5 taxa (1.4%), Veronica L. 5 taxa (1.4%), and Vicia L. 5 taxa (1.4%). The number of endemic taxa in the flora of Beyağaç (Denizli) are 39 (10.7%). Their threat categories were given according to “Red Data Book of Turkish Plants”. It is found 21 taxa in LC category, 4 taxa in NT category, 5 taxa in VU category, 6 taxa in EN category and 2 taxa in CR category.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121626826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Determination of chemical content of Lemna minor L. by GC-MS and investigation of antioxidant activity","authors":"Sema YİYİT DOĞAN, S. Atasağun, M. Ergönül","doi":"10.53447/communc.1122558","DOIUrl":"https://doi.org/10.53447/communc.1122558","url":null,"abstract":"Lemna minor L. has been traditionally used for a long time for its analgesic, antipyretic, vitamin C supplement, astringent, antipruritic effects. Although there are many heavy metal removals using L. minor, unfortunately, biological activity studies are very limited. In this study, the chemical content and total phenol content, DPPH removal, metal chelation (Fe2+) and β-Carotene-lycopene methods of the L. minor macrophyte we obtained from Turkey were determined by GC-MS. The results of the study showed that our plant contains 25 different essential oils and has a high phenol content. In addition, 72% DPPH removal of L.minor was determined when it had 71% iron chelating ability. As a result of our study, it has been revealed that the L. minor we use is an effective antioxidant. It is thought that its usability in the fields of food and medicine can be investigated with further studies.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131493648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant activities of Turkish extra virgin olive oils","authors":"Sakou Seydou, Gül Koca, Okan Onar, Ö. Yildirim","doi":"10.53447/communc.1069705","DOIUrl":"https://doi.org/10.53447/communc.1069705","url":null,"abstract":"Extra virgin olive oil is the highest grade of virgin olive oil derived by cold mechanical extraction without the use of solvents or refining methods. These olive oils are known for their composition in phenolic compounds that have antioxidant properties. This study aims to determine the total phenolic and flavonoid compounds and antioxidant activities of four Turkish extra virgin olive oil samples: Kilis yağlık, İzmir sofralık, Ayvalık, and Tavşan yüreği. The highest sample concentration used for the experiments was 4 mg/ml while 1 mg/mL was used for ABTS radical scavenging assay. The lowest total phenolic and flavonoid content was observed in Tavşan yüreği sample. All extra virgin olive oil samples showed scavenging activity against DPPH and ABTS free radicals. Extra virgin olive oil samples with high phenolic and flavonoid content presented more effective radical scavenging activity with low IC50 values. This study provides information about the phenolic content and antioxidant activities of four important Turkish olive oil samples.","PeriodicalId":249015,"journal":{"name":"Communications Faculty of Science University of Ankara Series C Biology Geological Engineering and Geophysical Engineering","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129131229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}