Zoological Science最新文献

Freshwater Colonization by a Scaleless Scale Worm: Pisione mizuchi sp. nov. (Annelida: Sigalionidae), from Sado Island, Japan. 日本佐渡岛无鳞鳞虫的淡水殖民：Pisione mizuchi sp. 11 .（节肢动物：鳞片虫科）。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250042

Satoshi Shimooka, Yoshito Mitsuo, Keiko Kishimoto-Yamada, Akihito Omori, Natsumi Hookabe, Naoto Jimi

引用次数: 0

A New Species of Branchiobdellidan Symbiotic on Freshwater Crabs from Southern Japan, with a Supplemental Re-Description of Cirrodrilus kawamurai. 日本南部淡水蟹类上一新种的共生枝鳃纲，并附川村卷蟹补充重新描述。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250083

Daisuke Uyeno, Hiroaki Tosuji, Akifumi Ohtaka

{"title":"A New Species of Branchiobdellidan Symbiotic on Freshwater Crabs from Southern Japan, with a Supplemental Re-Description of Cirrodrilus kawamurai.","authors":"Daisuke Uyeno, Hiroaki Tosuji, Akifumi Ohtaka","doi":"10.2108/zs250083","DOIUrl":"https://doi.org/10.2108/zs250083","url":null,"abstract":"Cirrodrilus osumi sp. nov. (Clitellata: Branchiobdellida: Branchiobdellidae) is described based on the specimens on the freshwater crab Geothelphusa exigua Suzuki and Tsuda, 1994 (Decapoda: Potamidae) collected from headwater areas in Osumi Peninsula, Kagoshima, southern Japan. Although the new branchiobdellidan resembles the continental East Asian Cirrodrilus kawamurai (Yamaguchi, 1934) in having four membranous large dorsal lobes on the peristomium, it differs by having shorter dorsal lobes and four pairs of small teeth in the jaws. Additionally, Ci. kawamurai is partially redescribed based on Yamaguchi's original slide collection. The Bayesian inference tree using partial mitochondrial cytochrome c oxidase subunit I sequences supported that the new species is genetically more closely related to a congener from the Korean Peninsula than to the endemic congeners from northern Japan. The finding of the new species in this study represents the record of the 12th species of the genus Cirrodrilus Pierantoni, 1905 from Japan, as well as the first record of a branchiobdellidan associated with freshwater crabs in East Asia.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"180-188"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic Relationships of the Scincella modesta Group (Squamata: Scincidae) in East Asia. 东亚麻麻属（鳞目：麻麻科）的系统发育关系。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250010

Yuki Koizumi, Tsutomu Hikida

{"title":"Phylogenetic Relationships of the Scincella modesta Group (Squamata: Scincidae) in East Asia.","authors":"Yuki Koizumi, Tsutomu Hikida","doi":"10.2108/zs250010","DOIUrl":"https://doi.org/10.2108/zs250010","url":null,"abstract":"The taxonomy of East Asian Scincella has been confused. Although the taxonomic status of some East Asian species was once synonymized Chinese S. modesta by Ouboter, this revision is no longer supported on the basis of morphological characteristics. However, molecular studies have not been conducted well, and the phylogenetic relationships of these East Asian species are not well understood. In this study, we tentatively labeled the nine species once synonymized with S. modesta as the \"S. modesta group,\" and used nuclear and mitochondrial DNA data from six of these species to reconstruct phylogenetic relationships. Within the S. modesta group, each species showed distinct lineages and four subclades were recognized in our study: (I) S. vandenburghi in Korea to Tsushima Island, Japan and S. modesta from central China, near the Changjiang River; (II) Scincella species of southern Ryukyus and Taiwan, S. boettgeri, and S. formosensis, etc.; (III) S. modesta from Ningbo (= Ningpo), Zhejiang, and Hong Kong; and (IV) Southwest Chinese Scincella, S. potanini, S. monticola, etc. This phylogenetic analysis revealed that S. modesta contained two distinct lineages at the species level. The estimated divergence dates imply that speciation of the S. modesta group began around the Early Miocene, with subclade IV diverging first, followed by III, and I and II diverged around the Late Miocene. Then, it is considered that this group diversified during the Pliocene. In addition, we propose a taxonomic suggestion for subspecies of S. modesta.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"151-161"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Relaxin-Like Gonad-Stimulating Peptide on Ovaries of the Sea Cucumber Holothuria leucospilota. 松弛素样性腺刺激肽对海参卵巢的影响。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250095

Masatoshi Mita, Hidekazu Katayama, Paul A Wright, Scott F Cummins, Sumio Udagawa, Masato Kiyomoto

{"title":"Effect of Relaxin-Like Gonad-Stimulating Peptide on Ovaries of the Sea Cucumber Holothuria leucospilota.","authors":"Masatoshi Mita, Hidekazu Katayama, Paul A Wright, Scott F Cummins, Sumio Udagawa, Masato Kiyomoto","doi":"10.2108/zs250095","DOIUrl":"https://doi.org/10.2108/zs250095","url":null,"abstract":"Relaxin-like gonad-stimulating peptide (RGP), identified in starfish as a gonadotropin-like active peptide, has been shown to have a similar effect in sea cucumbers. Chemically synthesized RGP of the black sea cucumber, Holothuria leucospilota, (Hle-RGP) induced oocyte maturation and ex vivo spawning in ovarian fragments of the same species in the reproductive season. Therefore, this study established ovarian fragments of H. leucospilota as a bioassay model to determine the RGP concentration (EC50) required for 50% ex vivo spawning. The EC50 for Hle-RGP was approximately 7 nM. However, Hle-RGP could not induce germinal vesicle breakdown (GVBD) in defolliculated immature oocytes isolated from ovaries, suggesting that follicle cells are essential for Hle-RGP-directed oocyte maturation. When the ovaries of H. leucospilota were incubated with Hle-RGP for 6 h at 18°C, activity of a maturation-inducing hormone (MIH) was found in the media. Because approximately 50 follicle cells surround an oocyte, it is probable that follicle cells stimulated by Hle-RGP produce the MIH. In contrast, neither 1-methyladenine (1-MeAde) nor RGP of the starfish Patiria pectinifera (Ppe-RGP) had any effect on oocyte maturation in H. leucospilota. These results strongly suggest that an MIH is involved in the process of oocyte maturation in sea cucumbers, similar to starfish, but the MIH in sea cucumbers is not 1-MeAde.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"172-179"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Diversity in a Mite, Coleolaelaps longisetatus (Mesostigmata: Laelapidae), Associated with Polyphylla Beetles (Coleoptera: Scarabaeidae) in Japan. 日本多叶甲虫（鞘翅目：金龟子科）一种螨的遗传多样性。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250038

Yuito Obae, Shigenori Karasawa

{"title":"Genetic Diversity in a Mite, Coleolaelaps longisetatus (Mesostigmata: Laelapidae), Associated with Polyphylla Beetles (Coleoptera: Scarabaeidae) in Japan.","authors":"Yuito Obae, Shigenori Karasawa","doi":"10.2108/zs250038","DOIUrl":"https://doi.org/10.2108/zs250038","url":null,"abstract":"Symbioses between mites and beetles are ubiquitous, but the relationships among mites and leaf-feeding, free-living beetle species have not been well studied. To clarify the relationships between a phoretic mite, Coleolaelaps longisetatus, and leaf-feeding, free-living Polyphylla beetle species in Japan, we determined phoretic rates of mites and clarified the patterns of genetic diversification of the mite in relation to the host beetle species. Totals of 252 P. albolineata, 31 P. laticollis, and 44 P. schoenfeldti were collected from 55, 14, and 10 sites, respectively. Coleolaelaps longisetatus was found on 224 P. albolineata (89%), 28 P. laticollis (90%), and 42 P. schoenfeldti (95%). No geographic pattern was observed in phoretic rates of mites. Phylogenetic analyses indicated that the C. longisetatus collected from each host beetle species formed monophyletic groups, suggesting that the diversification process of the host beetles affected the divergence of the phoretic mites. Haplotype network analyses showed that there was a roughly geographic pattern in genetic diversity within the mite clades, which may reflect the dispersal abilities of the host beetles.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"129-136"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levinaespira georgesnyderi gen. et sp. nov., a New Deep-Sea Snail (Gastropoda: Neomphalidae) from Eastern Pacific Methane Seeps. 东太平洋甲烷渗漏区深海新螺（腹足纲：新螺科）。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250078

Charlotte A Seid, Sonja Huč, Yasunori Kano, Anders Warén, Greg W Rouse

{"title":"Levinaespira georgesnyderi gen. et sp. nov., a New Deep-Sea Snail (Gastropoda: Neomphalidae) from Eastern Pacific Methane Seeps.","authors":"Charlotte A Seid, Sonja Huč, Yasunori Kano, Anders Warén, Greg W Rouse","doi":"10.2108/zs250078","DOIUrl":"https://doi.org/10.2108/zs250078","url":null,"abstract":"Deep-sea methane seeps host diverse and abundant animal communities, including an extensive undescribed diversity of small cryptic invertebrates. We describe a new genus and species of neomphalid snail, Levinaespira georgesnyderi gen. et sp. nov., from the Mound 12 methane seep at 1000 m depth on the Pacific margin of Costa Rica, and we report a closely related singleton specimen from the Hydrate Ridge seep off Oregon, USA, at ca. 600 m depth. Molecular phylogenies based on the mitochondrial cytochrome c oxidase subunit I (COI) gene and the complete mitochondrial genome support the placement of the new genus within Neomphalidae (Gastropoda: Neomphalida), a group originally described from hydrothermal vents. Levinaespira georgesnyderi gen. et sp. nov. is separated from species in other described genera by at least 15.2% COI distance. It is morphologically distinctive in having lateral projections on the anterior foot, a lobe-shaped right cephalic lappet, and bilaterally symmetric cephalic tentacles without obvious reproductive modifications. This species represents the first genetically confirmed record of Neomphalidae at methane seeps and the shallowest known occurrence of this group at any environment. The new genus is named in honor of Professor Emerita Lisa Levin and the new species is named in memory of Collection Manager H. George Snyder (1931-1990) for their contributions to deep-sea invertebrate biology.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"189-201"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential Expression of Na⁺/K⁺-ATPase α -Subunit Isoforms in Functionally Distinct Types of Ionocytes in the Gills of Mozambique Tilapia. 莫三比克罗非鱼鳃中Na+/K+- atp酶α -亚基亚型在不同功能类型离子细胞中的差异表达。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250088

Hiroshi Miyanishi, Shotaro Maeda, Ritsuko Ohtani-Kaneko, Toyoji Kaneko

{"title":"Differential Expression of Na+/K+-ATPase α -Subunit Isoforms in Functionally Distinct Types of Ionocytes in the Gills of Mozambique Tilapia.","authors":"Hiroshi Miyanishi, Shotaro Maeda, Ritsuko Ohtani-Kaneko, Toyoji Kaneko","doi":"10.2108/zs250088","DOIUrl":"https://doi.org/10.2108/zs250088","url":null,"abstract":"Na+/K+-ATPase (Nka) is essential for maintaining body fluid homeostasis in teleost osmoregulatory epithelia. In the present study, we identified six Nka α-subunit isoforms, including those previously reported in Mozambique tilapia. Among the Nka α-subunit isoforms, nkaa1a, nkaa1b, nkaa1c, and nkaa3b were highly expressed in the gills. In the gills of freshwater (FW)-acclimated fish, the expression levels of nkaa1c, were the highest, and those of nkaa1a and nkaa1b were moderately expressed, whereas in the gills of seawater (SW)-acclimated fish, nkaa1b expression was the highest, followed by nkaa3b. We also investigated the expressions of the four Nka α-subunit isoforms in the four types of ionocytes by means of triple fluorescence staining with whole-mount in situ hybridization and immunocytochemistry. The Nka α-subunit isoforms showed differential expression in functionally distinct types of ionocytes in Mozambique tilapia. Only nkaa1c was expressed in type I ionocytes, which are considered to be in the immature or resting phase. Nkaa1a is the major isoform in type II ionocytes that absorb Na+ and Cl- through apically-located Na+/Cl- cotransporter 2 (Ncc2) in FW. Type III ionocytes, which absorb Na+ in exchange for H+ through apical Na+/H+ exchanger 3 (Nhe3) in FW, mainly expressed nkaa1b, with moderate expression of nkaa1a and nkaa1c. Type IV ionocytes that secrete Na+ and Cl- in SW expressed mainly nkaa1b and, secondarily nkaa3b. It should be noted that nkaa1b is the major Nka α-subunit isoform in both type III and type IV ionocytes, which are substantially the same cell type with functional plasticity.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"162-171"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temporal Changes in Infection by Myxobolus nagaraensis in Rhinogobius (Gobiidae) in the Nagara River Basin, Central Japan, from the 1960s to the 2020s. 20世纪60年代至20世纪20年代日本中部长原河流域长垣粘虫侵染虾蛄的时间变化

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250060

Yasunori Koya, Satone Matsuoka, Gen Ito

{"title":"Temporal Changes in Infection by Myxobolus nagaraensis in Rhinogobius (Gobiidae) in the Nagara River Basin, Central Japan, from the 1960s to the 2020s.","authors":"Yasunori Koya, Satone Matsuoka, Gen Ito","doi":"10.2108/zs250060","DOIUrl":"https://doi.org/10.2108/zs250060","url":null,"abstract":"Myxobolus nagaraensis, a parasitic cnidarian, was first isolated from a freshwater goby of the genus Rhinogobius collected from the Nagara River system in central Japan and was described as a new species in 2007. This study investigated the changes in parasitism of Rhinogobius species by M. nagaraensis in the Nagara River Basin. Rhinogobius specimens, collected between 1967 and 2020 and stored at the Lake Biwa Museum and Gifu Prefectural Museum, were visually examined for the presence or absence of swelling of the abdomen or caudal peduncle specific to M. nagaraensis infestation. No swelling was identified among the 45,018 specimens collected from the main stream; however, one R. flumineus specimen collected from the Itaya River in 2011 exhibited a swelling among 1274 specimens collected from the tributaries. The myxospores extracted from the abdominal swelling were identified as M. nagaraensis through microscopic observation. Interviews with 26 individuals yielded four sightings of parasitized specimens, all of which occurred after 2007. Thus, there have been almost no cases of Rhinogobius species parasitism in the main stream from 2007 to the present, suggesting that parasitism may have predominantly spread to tributaries after 2007. Reasons for the changes in parasitism rates over time were discussed, including the possibility that parasitism rates in the main stream may have been underestimated due to biases caused by the sampling methods.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"146-150"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency of Scleractinian-Specific eDNA Metabarcoding Using Specimens from Okinawa Churaumi Aquarium, Japan. 日本冲绳Churaumi水族馆标本核核虫特异性eDNA元条形码的效率研究。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs240100

Haruhi Narisoko, Fumihiko Nagata, Kanako Hisata, Manabu Fujie, Yuki Yoshioka, Masanori Nonaka, Noriyuki Satoh, Koki Nishitsuji

{"title":"Efficiency of Scleractinian-Specific eDNA Metabarcoding Using Specimens from Okinawa Churaumi Aquarium, Japan.","authors":"Haruhi Narisoko, Fumihiko Nagata, Kanako Hisata, Manabu Fujie, Yuki Yoshioka, Masanori Nonaka, Noriyuki Satoh, Koki Nishitsuji","doi":"10.2108/zs240100","DOIUrl":"https://doi.org/10.2108/zs240100","url":null,"abstract":"Scleractinian-specific eDNA metabarcoding (eDNA-M), based on amplification of mitochondrial 12S rDNA, has become an effective tool for comprehensive monitoring of zooxanthellate scleractinians at the generic level, but several questions remain about its efficiency. Taking advantage of coral culturing tanks at Okinawa Churaumi Aquarium, Japan, we examined the robustness of this metabarcoding technique. Our eDNA-M system can theoretically identify 53 scleractinian genera. Without knowing genera and numbers of colonies in a large, indoor exhibition tank, eDNA-M identified 29 genera in tank effluent and one genus that requires further bioinformatic improvement for detection. Later, aquarium staff informed us that the tank contains 37 genera. Colony numbers in the tank were also provided. Tank population data revealed that mitochondrial 12S rDNA of the seven missing genera had not been sequenced yet. Those seven taxa were sequenced thereafter, with the result that at the end, 36 of the 37 genera were identified. Even three genera with only one colony in the tank were detected. Surveys of outdoor culture tanks yielded comparable results, with additional genera that may have been detected due to contamination in incurrent natural seawater. We conclude that this scleractinian-specific eDNA metabarcoding is robust for coral monitoring, although further mitochondrial 12S rDNA sequences are needed for additional scleractinian genera.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"137-145"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morphology and Phylogeny of Notospermus psittacinus comb. nov. (Nemertea: Pilidiophora: Lineidae) from Mindanao, Philippines. 标题鹦鹉螺的形态与系统发育。11 .菲律宾棉兰老岛产（新目：栉蝇科：栉蝇科）。

IF 1 4区生物学

Zoological Science Pub Date : 2026-04-01 DOI: 10.2108/zs250101

Jamael Abato, Archie Along, Nonillon Aspe, Caryl Kaye Awa, Nick Anthony Burias, Laurence Calagui, Shirlamaine Irina Calagui, Meljan Demetillo, April Joy Jovita, Julius Anthony Leones, Allexc Mae Niez, Romell Seronay, Hiroshi Kajihara

{"title":"Morphology and Phylogeny of Notospermus psittacinus comb. nov. (Nemertea: Pilidiophora: Lineidae) from Mindanao, Philippines.","authors":"Jamael Abato, Archie Along, Nonillon Aspe, Caryl Kaye Awa, Nick Anthony Burias, Laurence Calagui, Shirlamaine Irina Calagui, Meljan Demetillo, April Joy Jovita, Julius Anthony Leones, Allexc Mae Niez, Romell Seronay, Hiroshi Kajihara","doi":"10.2108/zs250101","DOIUrl":"https://doi.org/10.2108/zs250101","url":null,"abstract":"This study provides a redescription of the lineid heteronemertean Notospermus psittacinus (Bürger, 1890) comb. nov. based on specimens collected from Mindanao, Philippines. The species was previously assigned to the genus Lineus Sowerby, 1805, and has not been reported since its original description from Ambon, Indonesia. No DNA barcode sequences based on reliably identified material from the type locality are currently available. Notospermus psittacinus comb. nov. differs from the other six named species of the genus in body coloration, but can be distinguished from N. annulatus (Grube, 1840) only by molecular and biogeographic evidence. Despite the observed morphological variations in our specimens, genetic distance analyses indicated that all represent a single species (cytochrome c oxidase subunit I [COI] p-distance = 0%-2.12% [n = 7], 16S ribosomal RNA [16S] p-distance = 0%-1.57% [n = 6]). Genetic divergence between N. annulatus and N. psittacinus comb. nov. was 11.39%-12.18% for the 16S gene and 5.79% for the histone H3 (H3) gene, supporting their status as distinct species. A multigene phylogenetic analysis based on 16S, 18S ribosomal RNA, 28S ribosomal RNA, COI, and H3 sequences showed that all N. psittacinus comb. nov. specimens formed a well-supported clade within the genus Notospermus Huschke, 1830. Although intra-generic relationships remain poorly resolved, this result corroborates the generic reassignment of the species from Lineus to Notospermus.","PeriodicalId":24040,"journal":{"name":"Zoological Science","volume":"43 2","pages":"202-211"},"PeriodicalIF":1.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147821594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0