中华预防医学杂志最新文献

{"title":"[Production of recombinant protein of Tyr p 32 from <i>Tyrophagus putrescentiae</i> and identifying its immunoreactivity].","authors":"D M Zhou, Y N Ren, Y F Liao, Y Zhou, Y B Cui","doi":"10.3760/cma.j.cn112150-20231225-00485","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20231225-00485","url":null,"abstract":"<p><p>The present study was aimed to produce the recombinant protein of <i>Tyrophagus putrescentiae</i> allergen component 32 (Tyr p 32) and to identify its immunoreactivity. The cDNA encoding Tyr p 32 was amplified from total RNA of <i>T. putrescentiae</i> and inserted into pET-28a (+) vector. The constructed plasmid pET-28a (+)-Tyr p 32 was transformed into BL21 (DE3) receptor cells. After being induced with IPTG, the recombinant protein was purified with Ni column, and then identified on SDS-PAGE and Western blotting. Serum of children with allergic asthma and(or) rhinitis was collected, IgE-ELISA and IgE-Western blotting were used to detect the binding rate of rTyr p 32 to human <i>T.putrescentiae-</i>positive serum. After human bronchial epithelial cells BEAS-2B being cultured with rTyr p 32, the expression levels of IL-6 and IL-8 cytokines was detected by ELISA and qRT-PCR, t-test was used for pairwise comparison between groups. The results showed that the cDNA length of Tyr p 32 was 885 bp. The sequence identity between Tyr p 32 and Der p 32, Der f 32 was 70.21% and 68.03%, respectively. According to SDS-PAGE and Western blotting, the molecular weight of rTyr p 32 was about 35 000 Da, which was consistent with the theoretical value. IgE-ELISA results showed that the positive rate of rTyr p 32 was 41.38% (12/29) against <i>T. putrescentiae</i>-positive serum. When BEAS-2B cells were cultured with rTyr p 32, the expression of IL-6 and IL-8 increased in the cell supernatant in a dose-dependent manner (<i>t</i>=-29.10,<i>P</i>=0.001 2;<i>t</i>=-33.69,<i>P</i>=0.000 9), which also significantly increased the mRNA expression levels of IL-6 and IL-8 (<i>t</i>=-9.15,<i>P</i>=0.011 7;<i>t</i>=-17.16,<i>P</i>=0.003 4). In conclusion, the recombinant protein rTyr p 32 was successfully prepared, which provides raw materials for component diagnosis and specific immunotherapy of allergic diseases.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1933-1939"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical features and prognosis of children with multiple Takayasu's arteritis complicated with carotid artery occlusion].","authors":"B P He, J M Lai","doi":"10.3760/cma.j.cn112150-20240602-00444","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240602-00444","url":null,"abstract":"<p><p>This study aimed to analyze the clinical characteristics and prognosis of Takayasu's arteritis (TA) with carotid artery occlusion in children. This study collected clinical data and follow-up information on the first diagnosis and treatment of c-TA combined with carotid artery occlusion in pediatric patients at the Children's Hospital affiliated with the Capital Institute of Pediatrics and Inner Mongolia Medical University Affiliated Hospital from 2013 to 2023. This study included four female patients with a mean age of (13.25±1.71) years old. The time from onset to diagnosis was (8.14±11.19) months. Their main clinical symptoms and signs were dizziness, headache, chest discomfort, aphasia, dyskinesia, impaired mobility of the limbs, hypertension, asymmetry of blood pressure in the limbs, vascular murmurs or tremors, weakened radial artery pulsation, and decreased muscle strength. All four children showed elevated levels of C-reactive protein and erythrocyte sedimentation rate. The medical imaging results showed that among the four patients, three had bilateral carotid artery occlusion, and one had unilateral carotid artery occlusion. There were three cases of cerebrovascular involvement, accompanied by arterial involvement in multiple parts of the body, mainly involving bilateral subclavian arteries, cephalic trunk arteries, bilateral renal arteries, bilateral iliac arteries, and middle cerebral arteries. Four children were effectively treated with glucocorticoids and immunosuppressants. These children were followed up for a period of (42.25±52.54) months, which showed that the original affected artery still had varying degrees of occlusion, with no progression from the pre-treatment period and no new arterial involvement. The condition of three children with pre-existing physical and language disorders gradually improved. In summary, children with c-TA combined with carotid artery occlusion can cause involvement of multiple blood vessels. Their clinical manifestations are mostly systemic symptoms and ischemia of corresponding supply organs caused by vascular lesions, which are prone to be combined with cerebral infarction, and their occluded blood vessels are difficult to recover.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"2033-2037"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Epidemiological characteristics and influencing factors of public health emergency events of varicella in the Beijing-Tianjin-Hebei region, 2006-2021].","authors":"X Y Liu, M M Wang, M Y You, P H Wang, T Q Wang, X M Chen, C D Xu, X D Li, L Wang, Y H Hu, D P Yin","doi":"10.3760/cma.j.cn112150-20240301-00171","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240301-00171","url":null,"abstract":"<p><p>To explore the epidemiological characteristics of public health emergency events (PHEE) of varicella in the Beijing-Tianjin-Hebei region and analyze its related influencing factors. Excel was used to organize the varicella data in the Beijing-Tianjin-Hebei region from 2006 to 2021, reported by the management information system of PHEE, to describe the epidemiological characteristics of varicella events. Spatial autocorrelation and spatial scanning methods were used to test and determine its spatial clusters. Geographic detectors were used to analyze the impact of socio-economic factors. From 2006 to 2021, there were 644 reported varicella events in the Beijing-Tianjin-Hebei region, with a total of 18 052 cases and an incidence rate of 2.78%. The number, duration and response time <i>M</i> (<i>Q</i>₁, <i>Q</i>₃) of each reported event were 22 (15, 35) cases, 19 (7, 34) days and 7 (4, 17) days, respectively. Hebei Province had a shorter response time and duration of events compared to Beijing and Tianjin. The most reported varicella events were in 2006 and 2007, with 112 and 106 events, respectively. By 2014, the number of events had decreased yearly, and there was a small peak from 2017 to 2019 between 2014 and 2021. From 2006 to 2021, the PHEE of varicella showed a seasonal bimodal distribution from March to June and from October to January of the following year, with peaks in May and December. There was a total of 500 reported varicella events in primary schools, including 218 events in rural primary schools (34%), 142 events in county and town primary schools (22%) and 140 events in urban primary schools (22%). The distribution of varicella events showed a positive spatial autocorrelation and strong spatial clustering, with <i>Moran's I</i> of 0.31. The Class 1 clustering area was centered in Kuancheng Manchu Autonomous County, Chengde City, with a radius of 207 km and included 58 districts (<i>LLR</i>=3 550.23, <i>RR</i>=3.78). The most explanatory factor among socio-economic factors was the proportion of the population aged 0-24 years old (<i>q</i>=0.22), and the interaction effect between each factor was stronger than the independent effect. Overall, there are differences in the level of handling varicella events across Beijing, Tianjin and Hebei. The main occurrence of varicella events is in primary schools, especially in rural areas. Varicella events exhibit spatial clustering. Population structure-related factors have a strong impact on the risk of the incidence of varicella events.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1999-2004"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Analysis of the distribution characteristics of allergen sIgE detection in patients with respiratory and skin mucosal diseases in a hospital in Shanghai City from 2022 to 2023].","authors":"B B Xuan, M Y Tan, H X Sun, J J Chen, L D Zhou, H H Zhang, J M Yao, Y J Wang, J P Lin, H M Sheng","doi":"10.3760/cma.j.cn112150-20240325-00247","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240325-00247","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Analyzing the distribution characteristics of allergen sIgE in the serum of patients with respiratory and skin mucosal diseases in Shanghai City, and to provide epidemiological characteristics and diagnostic basis for the prevention and treatment of allergic respiratory and dermo-mucous diseases in Shanghai City. Adopting cross-sectional research, a total of 3 822 patients who received treatment in Tongren Hospital, Shanghai Jiao Tong University School of Medicine from July 2022 to July 2023 due to respiratory diseases or skin and dermo-mucous symptoms were included. Among them, there were 1 456 males and 2 366 females, with an age range of 1-97 years old. The median age (interquartile range) was 33 (27, 44) years old. The sIgE was detected by using immunoblotting. Statistical analysis was conducted using SPSS 22.0 software, and the comparison of count data (rates) between groups was conducted using &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt; test. The results showed that a total of 3 377 (88.4%) cases among 3 822 patients were at least one allergen sIgE positive, and 72.9% (2 788/3 822) of them were multiple allergies sIgE positive. The top five allergen sIgE positive rates were Dermatophagoides pteronyssinus (37.9%, 1 447/3 822), Dermatophagoides farinae (32.1%, 1 225/3 822), milk (31.7%, 1 211/3 822), fungi (28.3%, 1 080/3 822), and Blomia tropicdis (23.8%, 909/3 822), with only milk was a kind of food allergen. The highest positive rates within the respiratory system disease group or dermo-mucous disease group were also these five allergens, without any difference in disease categories. The positive rates of cat dandruff, Humulusscandens, and juniper/birch in the respiratory system disease group were significantly higher than those in the skin and mucous membrane disease group, while the positive rates of shrimp/crab were relatively low (11.3% &lt;i&gt;vs&lt;/i&gt; 14.9%, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=9.616,&lt;i&gt;P&lt;/i&gt;=0.002). Whether in the respiratory system disease group or the dermo-mucous disease group, the positive rates of Dermatophagoides pteronyssinus in male patients were significantly higher than those of females(42.6% &lt;i&gt;vs&lt;/i&gt; 35.7%,41.0% &lt;i&gt;vs&lt;/i&gt; 34.4%), with statistical significance (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=12.515, &lt;i&gt;P&lt;/i&gt;&lt;0.001; &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=5.143, &lt;i&gt;P&lt;/i&gt;=0.023), And the three allergens, Dermatophagoides farinae, cat dander, and egg white allergens are also characterized by this feature.In addition, the positive rates of milk(33.8% &lt;i&gt;vs&lt;/i&gt; 30.1%, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=3.911, &lt;i&gt;P&lt;/i&gt;=0.048), shrimp/crab(13.2% &lt;i&gt;vs&lt;/i&gt; 10.0%, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=6.423, &lt;i&gt;P&lt;/i&gt;=0.014) in the respiratory system disease group were higher in males than in females, while in the dermo-mucous disease group, dog dander(20.5% &lt;i&gt;vs&lt;/i&gt; 14.6%, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=6.726, &lt;i&gt;P&lt;/i&gt;=0.010) and peanuts/soybeans(10.5% &lt;i&gt;vs&lt;/i&gt; 6.9%, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;&lt;/i&gt;=4.698, &lt;i&gt;P&lt;/i&gt;=0.030) showed this phenomenon. In both the respiratory system disease group and the dermo-mucous disease group, there ","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1902-1911"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expert consensus on the passive immunization prevention of infant respiratory syncytial virus infection in China].","authors":"","doi":"10.3760/cma.j.cn112150-20240826-00684","DOIUrl":"10.3760/cma.j.cn112150-20240826-00684","url":null,"abstract":"<p><p>Respiratory syncytial virus (RSV) infection, which has the very heavy disease burdens, is the most common viral pathogen that seriously threatens the health of infants and young children. In order to better guide and standardize the clinical diagnosis, treatment, monitoring and immunization prevention and control of RSV infection in China, the Chinese Preventive Medicine Association jointly organized multidisciplinary experts to form a special working group, drawing on the implementation experience of foreign passive immunization prevention of RSV, integrating the existing evidence and the latest progress as a problem oriented, and putting forward the expert consensus on the passive immunization prevention of infant RSV infection in China in the future. The aim is to answer some professional questions about RSV infection and its immunoprophylaxis, especially passive immunoprophylaxis, in order to provide reference for the practice of immunoprophylaxis for professionals in medical institutions, disease prevention and control institutions and community health service centers at all levels.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 ","pages":"1853-1865"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142393841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Changes and correlation between serum β-amyloid 1-42 and total bilirubin in patients with Alzheimer's disease].","authors":"X Y Wang, H Deng, W Li, Y F Zhou, S J Pan","doi":"10.3760/cma.j.cn112150-20240813-00652","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240813-00652","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the levels of β-amyloid 1-42 (Aβ1-42) and total bilirubin (TBIL) in serum of patients with Alzheimer 's disease (AD) and the relationship between them. &lt;b&gt;Methods:&lt;/b&gt; A case-control study was conducted to select 73 patients with AD who were hospitalized in Beijing Huilongguan Hospital from November 2023 to February 2024 as AD group, and 70 healthy controls (HC) were selected as HC group. The basic information of all subjects and the clinical information of AD patients were collected, and the levels of Aβ1-42 and TBIL were detected and compared between the two groups. The effects of Aβ1-42 and TBIL on AD were analyzed by binary logistic regression. Correlation analysis was used to analyze the relationship between TBIL and Aβ1-42 in AD group and HC group. According to the level of Aβ1-42 in AD patients, they were divided into Aβ1-42 elevated group and Aβ1-42 normal group. The differences of clinical data and TBIL levels between the two groups were compared. According to the quartile of TBIL in AD patients, they were divided into Q1 group, Q2 group, Q3 group and Q4 group. The correlation between TBIL and the risk of Aβ1-42 elevation was analyzed by binary logistic regression. The receiver operating characteristic (ROC) curve was drawn to analyze the ability of TBIL level to predict the increase of Aβ1-42 in AD patients. &lt;b&gt;Results:&lt;/b&gt; There was no significant difference in gender, marital status, education level, smoking and drinking between AD group and HC group (&lt;i&gt;P&gt;&lt;/i&gt;0.05), while the levels of Aβ1-42 and TBIL and age in AD group were significantly higher than those in HC group [101.10(71.20, 128.60) pg/ml/22.40(10.00, 39.60) pg/ml&lt;i&gt;, Z&lt;/i&gt;=-8.714, &lt;i&gt;P&lt;/i&gt;&lt;0.001;(11.00±3.22/8.07±3.00) μmol/L&lt;i&gt;, t&lt;/i&gt;=5.621, &lt;i&gt;P&lt;/i&gt;&lt;0.001;(77.14±8.20/68.30±10.27) years, &lt;i&gt;t&lt;/i&gt;=5.672, &lt;i&gt;P&lt;/i&gt;&lt;0.001]. For AD patients, the TBIL level in the Aβ1-42 elevated group was lower than that in the Aβ1-42 normal group [(10.05±2.94/11.66±3.28) μmol/L&lt;i&gt;, t&lt;/i&gt;=-2.148, &lt;i&gt;P&lt;/i&gt;=0.035], while there was no significant difference in other demographic and clinical data between the two groups (&lt;i&gt;P&lt;/i&gt;&gt;0.05). Binary logistic regression analysis showed that higher levels of Aβ1-42 (&lt;i&gt;OR&lt;/i&gt;=1.021, 95%&lt;i&gt;CI&lt;/i&gt;：1.010-1.032) and TBIL (&lt;i&gt;OR&lt;/i&gt;=1.505, 95%&lt;i&gt;CI&lt;/i&gt;：1.249-1.814), older age (&lt;i&gt;OR&lt;/i&gt;=1.083, 95%&lt;i&gt;CI&lt;/i&gt;：1.020-1.150) and female (&lt;i&gt;OR&lt;/i&gt;=4.348, 95%&lt;i&gt;CI&lt;/i&gt;：1.253-15.094) were risk factors for AD. Correlation analysis showed that TBIL was negatively correlated with Aβ1-42 in AD patients (&lt;i&gt;r=&lt;/i&gt;-0.322, &lt;i&gt;P&lt;/i&gt;=0.006). After adjusting the relevant covariates, binary logistic regression showed that compared with AD patients in Q1 group, the risk of Aβ1-42 elevation in AD patients in Q4 group was lower (&lt;i&gt;OR&lt;/i&gt;=0.052, 95%&lt;i&gt;CI&lt;/i&gt;：0.005-0.535, &lt;i&gt;P&lt;/i&gt;&lt;0.05), and TBIL was negatively correlated with the risk of Aβ1-42 elevation (&lt;i&gt;P&lt;/i&gt; trend&lt;0.05). ROC curve showed that the area under the curve (AUC) of TBIL in predicti","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1987-1993"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Current situation and demand for specialized development of allergy nursing in eighty-eight hospitals in Guangdong Province].","authors":"C Dai, J Su, Y L Xu, Q P Chen, X X Lin, Y N Xu, X L Liu, S Chen, Y X Xu, A Z Yan, Y Y Hu, L Wang, F F Yan, Q L Zhou","doi":"10.3760/cma.j.cn112150-20240125-00084","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240125-00084","url":null,"abstract":"&lt;p&gt;&lt;p&gt;To investigate the current situation and demand for specialized development of allergy nursing in 88 hospitals in Guangdong Province, as well as to provide evidence for the management of allergy nursing. From August to December 2023, a cross-sectional study was conducted to analyze the core managers of allergy nursing-related work in eighty-eight hospitals in Guangdong Province supported by the Allergy Nursing Branch of the Guangdong Nurses Association and the Nursing Group of the Allergy Branch of the Guangdong Medical Association. An online survey was conducted using the Delphi method to compile a questionnaire on the current status and demand for specialized development of allergy nursing in Guangdong Province. Chi-square tests and Fisher's exact probability method were used to analyze the differences in the current status of specialty technology applications and the development and demand of specialized nursing between hospitals with or without allergy specialty departments. The results showed that among the eighty-eight hospitals in the nineteen prefecture-level cities surveyed in Guangdong Province, 17 hospitals (19%) had established allergy specialty departments, all of which were top-tier tertiary hospitals. The establishment duration [&lt;i&gt;M&lt;/i&gt; (&lt;i&gt;Q&lt;/i&gt;&lt;sub&gt;1&lt;/sub&gt;, &lt;i&gt;Q&lt;/i&gt;&lt;sub&gt;3&lt;/sub&gt;)] was 3 (1, 10) years. The implementation rates of skin prick test (94%, 16/17), in-vitro examination (82%, 14/17), nasal endoscopy (82%, 14/17), subcutaneous specific immunotherapy (82%, 14/17), multidisciplinary consultation (65%, 11/17), fractional exhaled nitric oxide detection (47%, 8/17), skin patch test (41%, 7/17), and autologous serum skin test (35%, 6/17) in hospitals with allergy department were higher than those in hospitals without allergy department [59%, 42/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;7.462, &lt;i&gt;P=&lt;/i&gt;0.006)], [54%, 38/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;4.717, &lt;i&gt;P&lt;/i&gt;=0.030)], [51%, 36/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;5.599, &lt;i&gt;P&lt;/i&gt;=0.018)], [34%, 24/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;13.177, &lt;i&gt;P&lt;/i&gt;&lt;0.001)], [24%, 17/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;10.505, &lt;i&gt;P&lt;/i&gt;=0.001)], [24%, 17/71 (&lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;5.540, &lt;i&gt;P&lt;/i&gt;=0.019)], [11%, 8/71 (Fisher's exact test, &lt;i&gt;P&lt;/i&gt;=0.008)], [11%, 8/71 (Fisher's exact test, &lt;i&gt;P&lt;/i&gt;=0.025)]. In the development plan of allergy nursing in the next two years, hospitals that had set up allergy departments had a higher proportion in adding nurses (82%, 14/17, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;13.177, &lt;i&gt;P&lt;/i&gt;&lt;0.001), formulating specialized nurse training plans (71%, 12/17, &lt;i&gt;χ&lt;sup&gt;2&lt;/sup&gt;=&lt;/i&gt;3.980, &lt;i&gt;P&lt;/i&gt;=0.046), formulating group standards or clinical guidelines (41%, 7/17, Fisher's exact test, &lt;i&gt;P&lt;/i&gt;=0.046), and formulating assessment standards (53%, 9/17, Fisher's exact test, &lt;i&gt;P&lt;/i&gt;=0.002). The demand for all aspects of allergy specialty nursing knowledge and skills accounted for more than 50%, and it was not related to whether the hospital set up an allergy department. In conclusion, the specialized development of allergy nursing in ","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1926-1932"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A clinical analysis on the distribution characteristics of dermatophagoides pteronyssinus allergen components among children with allergic rhinitis and asthma in a hospital of pediatric in Shenzhen City from 2021 to 2024].","authors":"S J Zhuang, T T Fan, X Y Ruan, R L Lai, W J Yan, C Y Liu, Z W Lu, M F Huang, F H Yang, Y M Bao","doi":"10.3760/cma.j.cn112150-20240708-00545","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240708-00545","url":null,"abstract":"&lt;p&gt;&lt;p&gt;&lt;b&gt;Objective:&lt;/b&gt; To investigate the distribution characteristics and analyze the clinical significance of dermatophagoides pteronyssinus allergen components in children with allergic rhinitis and asthma in Shenzhen. &lt;b&gt;Methods:&lt;/b&gt; This study was a cross-sectional study. The clinical data of children with allergic rhinitis and asthma induced by dust mites admitted to the allergy clinic of Shenzhen Children's Hospital from 2021 to 2024 were collected and the serum sIgE levels of dermatophagoides pteronyssinus, dermatophagoides farinae (Der p, Der f) and dermatophagoides pteronyssinus components (Der p 1, Der p 2, Der p 10, Der p 23) were detected by magnetic bead chemiluminescence method. The correlation between dermatophagoides pteronyssinus allergen components and clinical data of children was analyzed. According to the diagnosis, the children were divided into allergic rhinitis (AR) group and AR with asthma (ARAS) group. According to the age, the children were divided into preschool age (5 years ≤age&lt;7 years), school age (7 years ≤age&lt;10 years) and adolescence (10 years ≤age≤15 years). The expression differences of dermatophagoides pteronyssinus components among AR group and ARAS group and different age groups were compared. &lt;b&gt;Results:&lt;/b&gt; A total of 314 children with allergic rhinitis and asthma caused by dust mites were included in the study, of whom 112 were male and 202 were female. There were 188 cases of AR and 126 cases of ARAS, aged 5-15 years, with a median age of 7.54 years and an average age of (8.02±2.24) years. BMI was 13.89-31.76 kg/m&lt;sup&gt;2&lt;/sup&gt;,the median BMI was 15.87 kg/m² and average BMI was (16.55±3.05) kg/m². There was not statistically significant difference in gender, age, BMI, blood eosinophils, blood basophils, FeNO, FVC and FEV1 between the AR group and the ARAS group (&lt;i&gt;P&lt;/i&gt;&gt;0.05). There was significant difference in FEV1/FVC and small airway function indexes MMEF, MEF75%, MEF50% and MEF25% between the AR group and the ARAS group (&lt;i&gt;P&lt;/i&gt;&lt;0.05). In the 314 children, the dermatophagoides pteronyssinus allergen components sensitization rates were in the order of Der p 1 (97.1%), Der p 2 (89.8%), Der p 23 (55.1%), Der p 10 (8.6%), and the difference in the positive rate was statistically significant (χ&lt;sup&gt;2&lt;/sup&gt;=658.31, &lt;i&gt;P&lt;/i&gt;&lt;0.001). There was not significant difference in Der p 1, Der p 2 and Der p 10 among children of different ages (&lt;i&gt;P&lt;/i&gt;&gt;0.05). There was significant difference in Der p 23 among children of different ages (χ&lt;sup&gt;2&lt;/sup&gt;=7.29, &lt;i&gt;P&lt;/i&gt;=0.03). A correlation analysis showed that Der p, Der f, Der p 1 and Der p 2 had a high positive correlation (&lt;i&gt;P&lt;/i&gt;&lt;0.001). Eosinophils are positively correlated with Der p, Der f, Der p 1, Der p 2, Der p 10 and Der p 23 (&lt;i&gt;P&lt;/i&gt;&lt;0.001). FeNO is positively correlated with Der p, Der f, and Der p 23 (&lt;i&gt;P&lt;/i&gt;&lt;0.05). Small airway function indicators MMEF, MEF50% and MEF25% are negatively correlated with Der p, Der f and Der p 1 (&lt;i&gt;P&lt;/i&gt;&lt;0.05). The sI","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1885-1893"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A preliminary study on the short-term effectiveness and safety of sublingual immunotherapy-spray for patients with respiratory allergy].","authors":"X Y Dai, H D Lou, X Y Wang, S Chen, J Zhang, H B Ding, J Li, L Cheng","doi":"10.3760/cma.j.cn112150-20240921-00761","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240921-00761","url":null,"abstract":"<p><p>To investigate the short-term effectiveness and safety of sublingual allergen immunotherapy with allergen sprays (SLIT-sprays) in Chinese patients with allergic rhinitis (AR) with or without asthma using real-world data. The retrospective cohort study included 100 patients who received SLIT-sprays in the ENT departments in Hainan Shulan (Boao) Hospital and Boao Super Hospital between October 2023 and August 2024. A questionnaire survey was conducted to collect clinical data on the effectiveness and safety of SLIT-sprays, examining the types and incidence of adverse events (AEs) during treatment, treatments after the occurrence of AEs, and changes in Visual Analog Scale (VAS) scores before and after SLIT-sprays. Self-reports from 100 patients were collected. The results showed that the average treatment duration for the 100 patients was (90.7±58.9) days, median 78.5 days. Using changes in VAS scores as the effectiveness assessment, the average VAS score increased by 4.2 (95%<i>CI:</i> 4.06-4.34). The incidence of AEs during the SLIT-sprays was 17.0% (17/100), all of which were mild to moderate local reactions, with no serious AEs reported. There were no significant differences in AE incidence among patients with different diseases (AR or AR with asthma and asthma alone) (χ<i>²</i>=1.831,<i>P</i>>0.05), different age group (χ<i>²</i>=1.477,<i>P</i>>0.05), different types of allergen extracts (χ<i>²</i>=1.613,<i>P</i>>0.05), or the number of allergen extracts used (patients using one or two allergen extracts) (Fisher's exact test,<i>P</i>>0.05). In conclusion, Chinese patients showed good safety and tolerability to SLIT-sprays, with all AEs being mild to moderate local reactions and no serious or systemic AEs occurring. Patients reported positive subjective evaluations of the early treatment effects.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1921-1925"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142878216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of iron deficiency anemia and black stain on the microbial community of dental plaque in young children with early childhood caries].","authors":"S Bian, R Han, H Xiao, J Yue, S T Wang, H Z Lin, L Ma","doi":"10.3760/cma.j.cn112150-20240628-00522","DOIUrl":"https://doi.org/10.3760/cma.j.cn112150-20240628-00522","url":null,"abstract":"<p><p><b>Objective:</b> To analyze the effect of Iron deficiency anemia (IDA) and Black stain (BS) on the microbial community of dental plaque. <b>Methods:</b> A total of 136 preschool children aged 3 to 6 years old from 12 kindergartens in Shinan District and Shibei District of Qingdao City were investigated by using a cluster sampling method from April to May 2019. They were divided into two groups based on oral examination: the early childhood caries (ECC) group and the caries-free (CF) group. According to whether they had IDA and BS, they were further divided into four groups: the IDA with caries (IDA-ECC) group, the non-IDA with caries (NIDA-ECC) group, the BS without caries (BS-CF) group and the non-BS without caries (NBS-CF) group. The gingival plaque of the study subjects was collected. The 16S rRNA gene was sequenced by using Illumina MiSeq high-throughput sequencing technology. The composition, community structure and different bacteria genera of the microbial communities between the groups were compared and analyzed. Potential biomarkers within each group were further identified by linear discriminant analysis of effect size (LEfSe). <b>Results:</b> The 136 children were aged (5.11±0.87) years old, with 80 boys (58.82%). There were statistically significant differences in the microbial composition, structure and function of oral plaque between the ECC and CF groups (<i>P</i><0.05). There were statistically significant differences in the microbial richness and diversity of oral plaque between the IDA-ECC and NIDA-ECC groups (<i>P</i><0.05). There was no significant difference in microbial diversity index between the BS-CF and NBS-CF groups (<i>P</i>>0.05). The LEfSe analysis results showed that 41, 31 and 9 taxa with different relative abundance were identified between the ECC and CF groups, IDA-ECC and NIDA-ECC groups, and BS-CF and NBS-CF groups, respectively. <b>Conclusion:</b> IDA and BS have an effect on the microecological diversity and microbial community function of oral plaque in young children with early childhood caries.</p>","PeriodicalId":24033,"journal":{"name":"中华预防医学杂志","volume":"58 12","pages":"1976-1986"},"PeriodicalIF":0.0,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}