Zemdirbyste-agriculture最新文献_第10页

Candidate-gene expression patterns in diploid and tetraploid Lolium multiflorum spp. multiflorum cultivars under water deficit 水分亏缺条件下二倍体和四倍体何乐花候选基因的表达模式

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.046

O. Akinroluyo, V. Kemešytė, K. Jaškūnė, G. Statkevičiūtė

{"title":"Candidate-gene expression patterns in diploid and tetraploid Lolium multiflorum spp. multiflorum cultivars under water deficit","authors":"O. Akinroluyo, V. Kemešytė, K. Jaškūnė, G. Statkevičiūtė","doi":"10.13080/z-a.2021.108.046","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.046","url":null,"abstract":"Drought is a critical abiotic stress that disrupts redox homeostasis and is detrimental to plant development. To respond to drought, plants have developed complex mechanisms that allow them to perceive and counter abiotic stresses rapidly. Polyploidy gives rise to many gene expression changes allowing plants to adapt to a broader ecological range. The present study was designed to compare the expression of drought-related genes in diploid cultivars and induced tetraploid lines of Westerwolths ryegrass (Lolium multiflorum ssp. multiflorum). Plants were grown for three weeks in the growth chambers and were subjected to 5 days of water deficit. The relative expression of candidate genes was checked at three-time points, and the relative water content (RWC) was also calculated. The results of the experiment showed critical differences in response to water deficit periods between the diploid cultivars and their respective induced tetraploid lines. The induced tetraploid lines showed a significantly higher expression of drought-related genes than their diploid parents and had higher RWC. Also, out of the genes investigated, dehydrin is the most responsive gene to water deficit periods in Westerwolths ryegrass. Understanding the differential expression pattern of genes involved in drought response provides insight into how cytotypes respond to water deficit periods.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"23 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86985581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum 莴苣内生细菌对菌核菌白霉病拮抗能力的测定

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.039

S. Soylu, M. Kara, A. Uysal, Ş. Kurt, E. M. Soylu

{"title":"Determination of antagonistic potential of endophytic bacteria isolated from lettuce against lettuce white mould disease caused by Sclerotinia sclerotiorum","authors":"S. Soylu, M. Kara, A. Uysal, Ş. Kurt, E. M. Soylu","doi":"10.13080/z-a.2021.108.039","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.039","url":null,"abstract":"In this study, biocontrol efficiency of endophytic bacterial isolates obtained from the leaf lettuce (Lactuca sativa L.) healthy plants was investigated against white mould disease agent Sclerotinia sclerotiorum in vitro and in vivo conditions. Antagonistic efficiency of endophytic bacterial isolates was determined to inhibit mycelial growth and sclerotial germination, suppress disease incidence caused by S. sclerotiorum. A total of 48 endophytic bacterial isolates were isolated from different tissues of lettuce healthy plants. Mycelial growth and germination of sclerotia of S. sclerotiorum in vitro inhibited 18 bacterial isolates. Bacillus subtilis and B. amyloliquefaciens isolates were found to be the most efficient ones, which significantly inhibited the mycelial growth by 68.1–83.1%, germination of sclerotia by 82.7–89.6% and suppressed disease incidence by 55.7–75% caused by S. sclerotiorum. In addition, the fungal mycelium close to the inhibition zone in dual culture was denser and darker in colour. B. subtilis and B. amyloliquefaciens isolates caused significant morphological alterations in hyphae such as hyphal shrivelling and perforation close to the inhibition zone in dual culture. Significant suppression in the mycelial growth, sclerotial germination and disease incidence caused by endophytic bacterial isolates indicate that isolates of B. subtilis and B. amyloliquefaciens could be considered as possible biocontrol agents against soil-borne fungal diseases.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"36 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76819721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Botrytis four species are associated with chocolate spot disease of faba bean in Latvia 拉脱维亚蚕豆巧克力斑疹病与4种葡萄孢菌有关

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.038

B. Bankina, F. Stoddard, J. Kaneps, E. Brauna-Morževska, G. Bimšteine, Ingrīda Neusa-Luca, A. Roga, D. Fridmanis

{"title":"Botrytis four species are associated with chocolate spot disease of faba bean in Latvia","authors":"B. Bankina, F. Stoddard, J. Kaneps, E. Brauna-Morževska, G. Bimšteine, Ingrīda Neusa-Luca, A. Roga, D. Fridmanis","doi":"10.13080/z-a.2021.108.038","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.038","url":null,"abstract":"Faba bean (Vicia faba L.) is gaining importance as a crop in northern Europe. In this region, the most important disease of faba bean is chocolate spot disease, attributed to the pathogen Botrytis fabae. However, other Botrytis species have been found to contribute to the disease. Hence, it was decided to isolate fungi from faba bean plants showing symptoms of chocolate spot disease in Latvia, identify the Botrytis species using the DNA sequences of three definitive genes, evaluate the morphological diversity of the isolates in vitro and, finally, to determine the pathogenicity of the isolates in a detached-leaf test. In addition to B. fabae, B. cinerea, B. pseudocinerea and B. fabiopsis were all identified. Phylogenetic analysis of the DNA sequences put all the obtained 44 isolates unequivocally into clusters with known examples of each species. Every species showed wide diversity in its in vitro colour, texture and growing pattern of mycelium, production of sclerotia and pigmentation of the growing medium with much overlap between species showing that this method is not adequate for species discrimination. B. fabae produced the largest lesions on infected leaves, followed closely by B. pseudocinerea and B. cinerea, while B. fabiopsis produced much smaller lesions. The results show that chocolate spot disease of faba bean is attributable to Botrytis four species in northern Europe. This knowledge needs to be considered when controlling the disease by genetic or agronomic means.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"28 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75203446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Interaction of host factors in response to yeast K2 toxin stress – attractiveness for plant protection 寄主因子对酵母K2毒素胁迫响应的相互作用——植物保护的吸引力

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.040

I. Vepštaitė-Monstavičė, Juliana Lukša, E. Servienė

{"title":"Interaction of host factors in response to yeast K2 toxin stress – attractiveness for plant protection","authors":"I. Vepštaitė-Monstavičė, Juliana Lukša, E. Servienė","doi":"10.13080/z-a.2021.108.040","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.040","url":null,"abstract":"Killer toxin-producing yeasts are important in agriculture, as they may be used for the biological control of field and post-harvest bacterial and fungal diseases of plants. The foundation for the development and application of killer yeast as plant protection agents is understanding the mechanisms underlying killer toxin-conditioned biocontrol activity and the tolerance to toxin-driven stress. This study aimed to determine the interactions between genetic effectors of cell wall integrity (CWI) and high osmolarity glycerol (HOG) pathways under the stress conditions caused by Saccharomyces cerevisiae dsRNA-originated K2 toxin. Genetic interaction studies have used different measures of fitness including the relative growth rate and toxin susceptibility. During the research, 12 double deletion mutants were created by applying homologous recombination approach, and their growth parameters and response to toxin action were analysed. Most double gene deletion strains demonstrated insignificant (less than 30%) changes in growth rate compared to single gene mutants. Only elimination of HOG1 gene in strains Δspt3 and Δspt8 resulted in significant increase of the growth rate reaching about 0.6 h-1. K2 toxin sensitivity increased in most of the double mutants, independent of the involvement of gene products into the same or different signalling pathways thus pointing to weak interactions of gene products. HOG1 mutation alters the phenotype (from resistant to sensitive) of mutants Δrlm1, Δlrg1 and Δslm4 only and are epistatic to these CWI pathway effectors. In addition to the functional analysis, network of proteins involved in K2 toxin response was generated uncovering HOG and CWI players interconnected or acting through mediators. This study deepens insight into the K2 toxin response-modulated genetic interactions and provides data important for practical application of killer yeasts.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"26 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81791912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and functional prediction of long non-coding RNAs responsive to heat stress in heading type Chinese cabbage 结球型大白菜热胁迫响应长链非编码rna的鉴定及功能预测

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.047

S. Eom, H. Lee, S. Wi, Sung Kyeom Kim, T. Hyun

{"title":"Identification and functional prediction of long non-coding RNAs responsive to heat stress in heading type Chinese cabbage","authors":"S. Eom, H. Lee, S. Wi, Sung Kyeom Kim, T. Hyun","doi":"10.13080/z-a.2021.108.047","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.047","url":null,"abstract":"Global warming is a devastating force that considerably hinders the growth, quality and yield of heading type Chinese cabbage (Brassica rapa L. ssp. pekinensis). Recent research suggests that long non-coding ribonucleic acids (lncRNAs) play a role in response to abiotic and biotic stresses. In this study, a total of 278 lncRNAs belonging to intergenic, intronic, sense and natural antisense lncRNAs in the heading type Chinese cabbage using RNA-sequencing (RNA-seq) data were identified. Based on the analysis of the differentially expressed lncRNAs, 93 out of 278 lncRNAs were identified as heat-responsive lncRNAs. In total, 65 heat-responsive lncRNAs were predicted as putative targets and target mimics of B. rapa microRNAs (miRNAs). In addition, it was found that some identified lncRNAs play important role in response to heat stress via lncRNA-messenger RNA (mRNA) (lncRNA.Brassica_094-DnaJ protein and lncRNA.Brassica_181-REF4-related 1) co-expression, whereas some lncRNA-miRNA (lncRNA-Brassica_116-bra-miR164a and lncRNA-Brassica_205-bra-miR159) interactions are required for modulation of miRNA action. Taken together, these results provide the starting point for a detailed investigation of the physiological function of the lncRNA-dependent network in heading type Chinese cabbage.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"45 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73506373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Maize response to soil properties improved with beneficial microbes, humic acid and farmyard manure application 施用有益微生物、腐植酸和农家肥改善玉米对土壤性状的响应

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.041

I. Hussain, Ahmad Masood Khan, H. Akbar, Z. Hussain

{"title":"Maize response to soil properties improved with beneficial microbes, humic acid and farmyard manure application","authors":"I. Hussain, Ahmad Masood Khan, H. Akbar, Z. Hussain","doi":"10.13080/z-a.2021.108.041","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.041","url":null,"abstract":"To avoid environmental pollution, the useful and safe disposal of farmyard manure (FYM) was assessed during a two-year (2017 and 2018) field experiment using beneficial microbes (BM): 25 and 50 L ha-1, humic acid (HA): 3, 6 and 9 kg ha-1, and FYM: 10, 15 and 20 Mg ha-1 compared with a control (without BM, HA or FYM). Doubling the amount of BM from 25 to 50 L ha-1 delayed phenological events of maize (Zea mays L.) by one day, decreased soil total nitrogen (Ntot) content (12.6%) but improved soil bulk density (BD) (1.25 Mg m -3), electrical conductivity (EC) (1.90 dS m-1) and mineral N (Nmin) content (33.89 mg kg -1 soil). Increasing HA application from 3 to 9 kg ha-1, physiological maturity of maize delayed by 2 days but increased soil EC (0.11 dS m-1), BD (1.63%), Nmin (12.94%) and decreased soil Ntot (34.42%) content. With the FYM level increasing from 10 to 20 Mg ha -1, the phenological events were delayed, but soil properties, i.e., pH (−0.3 unit), EC (6.66%), BD (−5.83%), Nmin (28.14%) and Ntot (30.16%) content, were improved. Soil properties like EC and Ntot and Nmin contents showed significant positive correlation with maize leaf area, grain N content and grain yield. It was concluded that with addition of either 50 L ha-1 BM or 6–9 kg ha-1 HA, FYM mineralization increased, growth periods prolonged and soil fertility indices improved. Thus, for improving the Nmin availability and prolonged maize phenological duration for maximizing productivity, 20 Mg ha-1 FYM should be applied in combination with 50 L ha-1 BM and/or 6 kg ha-1 HA.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"6 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79582182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiency of alfalfa seed priming and rhizobial-based liquid inoculants in an Eutric Cambisol 紫花苜蓿种子和根瘤菌基液体接种剂在营养性Cambisol中的效果

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.043

A. Buntić, O. Stajković-Srbinović, M. Knežević, N. Rasulić, V. Ugrenović, D. Kuzmanović, D. Delic

{"title":"Efficiency of alfalfa seed priming and rhizobial-based liquid inoculants in an Eutric Cambisol","authors":"A. Buntić, O. Stajković-Srbinović, M. Knežević, N. Rasulić, V. Ugrenović, D. Kuzmanović, D. Delic","doi":"10.13080/z-a.2021.108.043","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.043","url":null,"abstract":"In the last few decades, peat-based bioinoculants have been proven as successful in alfalfa (Medicago sativa L.) seeds inoculation. However, the use of liquid inoculants or pre-inoculated seeds will greatly facilitate the sowing of alfalfa as a small seed legume. Therefore, this study evaluated the effect of four liquid formulations on the survival of Sinorhizobium (Ensifer) meliloti strain 218 during long-term storage. Sodium-alginate (SA) and polyvinylpyrrolidone (PVP) did not affect the growth of S. meliloti strain 218 and showed a significant positive effect on its survival in liquid bioinoculants up to 240 days of storage. The effect of seeds pre-inoculation on the efficiency of nitrogen (N) fixation in alfalfa plants in test tube and a two-year field experiment was measured. The field experiment was conducted in 2018 and 2019 in an Eutric Cambisol. The dry weight of alfalfa shoots and total N content significantly increased in pre-inoculated seeds with all media, compared to the control treatments without pre-inoculation and adding mineral N. In both years of cultivation, the highest total shoot dry weight was achieved by seeds pre-inoculation with SA and PVP 3 months before sowing. Furthermore, in these treatments, shoot dry weight significantly increased compared to the treatment with mineral N in the 2nd (2019) year. The results of the research showed that rhizobial bioinoculants with SA and PVP could be used as liquid bioinoculants as well as for successful pre-inoculation of alfalfa seeds.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"19 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75565286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The presence of pathogenic bacteria Pseudomonas syringae in cereals in Lithuania 立陶宛谷物中丁香假单胞菌的存在

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.037

J. Kelpšienė, D. Šneideris, D. Burokienė, S. Supronienė

{"title":"The presence of pathogenic bacteria Pseudomonas syringae in cereals in Lithuania","authors":"J. Kelpšienė, D. Šneideris, D. Burokienė, S. Supronienė","doi":"10.13080/z-a.2021.108.037","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.037","url":null,"abstract":"Pseudomonas syringae pathovars cause bacterial diseases of cereals in nearly all temperate and subtropical cerealgrowing regions. As being of low importance compared to fungal diseases, P. syringae has not been studied in cereal crops in Lithuania. However, leaf blights and glume discolorations, uncharacteristic of fungal diseases, were found relatively frequently in the fields. Therefore, the study aimed to identify the occurrence of P. syringae in cereals grown in Lithuania. In this study, cereal crops were tested for the presence of plant pathogen P. syringae. In total, 452 symptomatic plant samples of winter wheat, winter triticale, spring wheat and spring barley were collected in 2013–2015. Symptoms of leaf blight and basal glume blotch on grain were more frequently detected in spring and winter wheat crops than in winter triticale and spring barley. Symptomatic leaves and grain yielded 113 P. syringae strains (51 from leaves and 62 from grain), but only 13 strains (10 from wheat, 2 from triticale and 1 from barley) were pathogenic to host plants when tested by spray method (SM). These strains were isolated from grain (10 strains) and leaves (3 strains) of all sampled plant species originating from different districts of the country. Based on 16S rRNA gene sequence analysis, leaf injection method (LIM) and origin of pathogenic strain, it can be assumed that P. syringae pv. atrofaciens is the dominat causal agent of bacterial diseases of cereals, particularly basal glume","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"65 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84010546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sugar beet fertilisation for sustainable yield under climate change conditions 气候变化条件下甜菜可持续产量的施肥

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.045

V. Ivanina, R. Shapovalenko, O. Strilets, S. Senchuk

{"title":"Sugar beet fertilisation for sustainable yield under climate change conditions","authors":"V. Ivanina, R. Shapovalenko, O. Strilets, S. Senchuk","doi":"10.13080/z-a.2021.108.045","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.045","url":null,"abstract":"The aim of this study was to determine how different fertiliser systems affect sugar beet (Beta vulgaris L.) productivity, water-use efficiency (WUE) index, nutrient uptake and balance and establish the efficient fertilisation of the plant. A randomized experimental design with four replications as factorial arrangement with four treatments: (1) without fertilisers (control), (2) mineral fertilisation (MF), (3) alternative organic-mineral fertilisation (OMF) and (4) organic-mineral fertilisation supplemented with boron (B) (OMF+B), was used. The results showed that sugar beet root yield, gross and white sugar yield were significantly affected (P < 0.05) by all fertilisation treatments. The highest average of the aforementioned parameters was obtained in OMF+B treatment: 63.5, 10.73 and 8.86 t ha-1, respectively, that included combined application of the mineral fertilisers and winter wheat straw plus B twice foliar applied. OMF+B had a more pronounced effect on sugar beet productivity in the year of hot and moderately humid growing season (2018) than in dry (2017) and moderate (2019) years. OMF+B resulted in a positive nutrient balance in the soil and the highest WUE index (44.7 kg ha-1 mm-1), provided efficient use of water and the sustainability of sugar beet cultivation under climate change conditions, while mineral fertilisation led to nutrient imbalance and low stability. The accumulation of sugar in the roots mainly depended on the dry weather in September. The driest September of 2019 contributed to the highest sugar content in the roots – 18.2–18.5%, while moderately and too humid weather in September 2017 and 2018 caused a significant decrease (P < 0.05) in sugar content to 17.0–17.5% and 14.9–15.2%, respectively.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"181 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75708861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In vitro propagation of Passiflora edulis through internodal segments as affected by medium composition 培养基成分对西番莲节段离体繁殖的影响

IF 0.9 4区农林科学

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI: 10.13080/z-a.2021.108.048

N. Burbulis, A. Blinstrubienė, Aistis Petruskevicius

{"title":"In vitro propagation of Passiflora edulis through internodal segments as affected by medium composition","authors":"N. Burbulis, A. Blinstrubienė, Aistis Petruskevicius","doi":"10.13080/z-a.2021.108.048","DOIUrl":"https://doi.org/10.13080/z-a.2021.108.048","url":null,"abstract":"The aim of the experiment was to evaluate the effect of the basal medium and the type and concentration of cytokinin on direct regeneration from internodal segments of passion flower (Passiflora edulis Sims). Internodal segment explants were cultured on the Murashige and Skoog (MS) and woody plant medium (WPM) without growth regulators or supplemented with 0.5–3.0 mg L-1 of 6-benzylaminopurine (BAP), thidiazuron (TDZ) or zeatin (ZEA). The cultivation of the internodal segment explants on media supplemented with cytokinins resulted in direct organogenesis without a callus phase. The shoot formation frequency and shoot number per explant were strongly influenced by the type and concentration of cytokinin. MS medium supplemented with BAP resulted in a shoot formation frequency that was higher in comparison with TDZ but significantly lower as compared to 0.5–2.5 mg L-1 ZEA. On the other hand, on WPM supplemented with BAP the shoot formation frequency was significantly lower in comparison with that for analogous ZEA and TDZ concentrations. The shoot formation frequency and shoot number per explant were strongly influenced by interactions of the basal medium and type and concentration of cytokinin. The MS medium supplemented with 2.0 mg L-1 ZEA resulted in the highest shoot formation frequency, while the highest shoot number per explant was obtained on WPM supplemented with 1.5 mg L-1 ZEA. The highest shoot formation frequency (98.1%) with the highest number of shoots per explant (9.53) was observed on the WPM supplemented with 1.5 mg L-1 ZEA. The formed shoots were rooted on MS medium containing 1⁄2 macro and micro salts supplemented with 2.0 mg L-1 IBA (indole-3-butyric acid); about 92% of them survived and grew normally with true-to-type morphology.","PeriodicalId":23946,"journal":{"name":"Zemdirbyste-agriculture","volume":"1 1","pages":""},"PeriodicalIF":0.9,"publicationDate":"2021-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80786687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0