Identification and functional prediction of long non-coding RNAs responsive to heat stress in heading type Chinese cabbage

IF 0.8 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI:10.13080/z-a.2021.108.047

S. Eom, H. Lee, S. Wi, Sung Kyeom Kim, T. Hyun

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引用次数: 2

Abstract

Global warming is a devastating force that considerably hinders the growth, quality and yield of heading type Chinese cabbage (Brassica rapa L. ssp. pekinensis). Recent research suggests that long non-coding ribonucleic acids (lncRNAs) play a role in response to abiotic and biotic stresses. In this study, a total of 278 lncRNAs belonging to intergenic, intronic, sense and natural antisense lncRNAs in the heading type Chinese cabbage using RNA-sequencing (RNA-seq) data were identified. Based on the analysis of the differentially expressed lncRNAs, 93 out of 278 lncRNAs were identified as heat-responsive lncRNAs. In total, 65 heat-responsive lncRNAs were predicted as putative targets and target mimics of B. rapa microRNAs (miRNAs). In addition, it was found that some identified lncRNAs play important role in response to heat stress via lncRNA-messenger RNA (mRNA) (lncRNA.Brassica_094-DnaJ protein and lncRNA.Brassica_181-REF4-related 1) co-expression, whereas some lncRNA-miRNA (lncRNA-Brassica_116-bra-miR164a and lncRNA-Brassica_205-bra-miR159) interactions are required for modulation of miRNA action. Taken together, these results provide the starting point for a detailed investigation of the physiological function of the lncRNA-dependent network in heading type Chinese cabbage.

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结球型大白菜热胁迫响应长链非编码rna的鉴定及功能预测

全球变暖是严重影响抽穗型大白菜生长、品质和产量的破坏性因素。学报)。最近的研究表明，长链非编码核糖核酸(lncRNAs)在应对非生物和生物胁迫中发挥着重要作用。本研究利用RNA-seq (RNA-seq)数据，从结球型大白菜中共鉴定出278个基因间、内含子、正义和天然反义lncRNAs。通过对差异表达lncrna的分析，278个lncrna中有93个被鉴定为热响应性lncrna。总共有65种热响应lncrna被预测为rapa microRNAs (miRNAs)的推测靶标和靶标模拟物。此外，还发现一些已鉴定的lncRNA通过lncRNA-信使RNA (mRNA) (lncRNA)在热应激响应中发挥重要作用。Brassica_094-DnaJ蛋白和lncRNA。brassica_181 - ref4相关的1)共表达，而一些lncRNA-miRNA (lncRNA-Brassica_116-bra-miR164a和lncRNA-Brassica_205-bra-miR159)相互作用需要调节miRNA的作用。综上所述，这些结果为详细研究结穗型大白菜lncrna依赖网络的生理功能提供了起点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zemdirbyste-agriculture AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

1.80

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Zemdirbyste-Agriculture is a quarterly scientific journal which covers a wide range of topics in the field of agricultural sciences, agronomy. It publishes articles of original research findings in the English language in the field of agronomy (soil and crop management, crop production, plant protection, plant breeding and genetics, biotechnology, plant nutrition, agrochemistry, soil science, microbiology etc.) and related areas. Articles are peer-reviewed. Review, debating papers as well as those of a methodological nature will also be considered.